Status
Please wait ...
Gene information
General information
TC009023 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid49: 1940728..1942167
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009023
    iBeetle screen iB_01459
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 6 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009023 RA
      MSLRLANLT TIRLLQDNL PPLLVPQLR ERHTKHWDP 
      KWKSLRRQK VMKVDLPNF REKPEDLTE EQVRAKLKE 
      HGILPPRPW VEKPFFISS TGGIFEPYV PPEGDGKIS 
      PIHMQGAKQ KLQFLEKKS KTMMAIRKI RMFEEDFDT 
      TAFCKEAEN IYVKMHELM AKKDWDNIT DYVTERAYP 
      EVIHNVENK TIHWKLIKN IELPRVVHA RCTDVITKE 
      NVFGQTTVR FHTQQMLAV YDRFGRLMH GSEIIAKDV 
      LEYIVFEKH LANVYGKWR IQGKIIPDW LPPKEPSAT 
      TYLKPAEET PPALPAEVP PPEEKEISK ESNP
    CDS sequence
    • >TC009023 RA
      atgtcttta cgccttgcc aatttgacc acaattcgc 
      ctgcttcag gacaacttg cccccctta ctagttccc 
      cagcttcgt gaaagacac accaaacac tgggacccc 
      aaatggaaa agcctccgg cgtcaaaag gtcatgaag 
      gtagactta ccgaatttt cgcgaaaag ccggaagac 
      ttaactgag gaacaagtg cgtgctaag ttgaaagag 
      catgggatt ttgccccca cgcccgtgg gttgaaaag 
      ccctttttc ataagctcg actggtggt attttcgag 
      ccatacgta ccccctgag ggtgacggg aaaatctcc 
      cccattcac atgcagggg gctaaacag aagttgcag 
      tttttggaa aagaaatca aaaacgatg atggcgatt 
      agaaaaatt cgcatgttt gaggaggat ttcgatact 
      acggcgttt tgtaaagaa gctgagaat atttacgtg 
      aaaatgcac gagttaatg gcaaaaaaa gattgggat 
      aacatcacg gactatgtc actgaacgg gcgtatccg 
      gaggttatt cataatgtg gaaaataaa actattcac 
      tggaagttg attaaaaac atcgagttg ccaagggtt 
      gtacacgcg aggtgcact gatgtaata acgaaagaa 
      aacgttttt ggccaaact acagtcagg tttcacaca 
      caacagatg ttggcagtg tatgaccga tttggccgt 
      ttgatgcat gggagcgaa ataatcgca aaggacgtt 
      ttagaatat atagttttc gaaaaacat ttggccaat 
      gtttatgga aaatggcgc attcaagga aaaatcatc 
      ccggattgg ctcccgcct aaagaaccc tccgcgacc 
      acttatctg aagccggct gaggaaaca cccccagcc 
      ctaccagcc gaagtacca ccacccgaa gaaaaggag 
      atctccaaa gaaagtaat ccttaa
    mRNA sequence
    • >TC009023 RA
      atgttggca aaagatttg gtaattgtc agttacaga 
      attctaggt tagaatatt tgtgaacaa atcttgaaa 
      tagaaagct taattccca aaaccgata cagcaatgt 
      ctttacgcc ttgccaatt tgaccacaa ttcgcctgc 
      ttcaggaca acttgcccc ccttactag ttccccagc 
      ttcgtgaaa gacacacca aacactggg accccaaat 
      ggaaaagcc tccggcgtc aaaaggtca tgaaggtag 
      acttaccga attttcgcg aaaagccgg aagacttaa 
      ctgaggaac aagtgcgtg ctaagttga aagagcatg 
      ggattttgc ccccacgcc cgtgggttg aaaagccct 
      ttttcataa gctcgactg gtggtattt tcgagccat 
      acgtacccc ctgagggtg acgggaaaa tctccccca 
      ttcacatgc agggggcta aacagaagt tgcagtttt 
      tggaaaaga aatcaaaaa cgatgatgg cgattagaa 
      aaattcgca tgtttgagg aggatttcg atactacgg 
      cgttttgta aagaagctg agaatattt acgtgaaaa 
      tgcacgagt taatggcaa aaaaagatt gggataaca 
      tcacggact atgtcactg aacgggcgt atccggagg 
      ttattcata atgtggaaa ataaaacta ttcactgga 
      agttgatta aaaacatcg agttgccaa gggttgtac 
      acgcgaggt gcactgatg taataacga aagaaaacg 
      tttttggcc aaactacag tcaggtttc acacacaac 
      agatgttgg cagtgtatg accgatttg gccgtttga 
      tgcatggga gcgaaataa tcgcaaagg acgttttag 
      aatatatag ttttcgaaa aacatttgg ccaatgttt 
      atggaaaat ggcgcattc aaggaaaaa tcatcccgg 
      attggctcc cgcctaaag aaccctccg cgaccactt 
      atctgaagc cggctgagg aaacacccc cagccctac 
      cagccgaag taccaccac ccgaagaaa aggagatct 
      ccaaagaaa gtaatcctt aaaaacata caaaaaata 
      tatatttta aattataat atacaattt tccaataa