Status
Please wait ...
Gene information
General information
TC009010 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8813097..8816434
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC009010
    iBeetle screen iB_01456

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009010 RA
      MLGRFGSLV PLVTQNVQQ QRGMATLKS ISIRLKSVK 
NIQKITQSM KMVSAAKYS RAERELKQT RPIGSGATQ 
FYEKAEISA PEDLPQKLL IAMTSDRGL CGAVHTSVA 
RLIRNELNN DQQNLKVVC VGDKSRLIL QRMYGKNII 
MACNEIGRL PPTFTDASK LADAILNSQ YAFGSGQIV 
FNRFKSVVS YSTQTLPVF SLAAVKTAA KLPVYDSLD 
DEVIQSYLE FSLASLLYY AMKEGACSE QSSRMTAMD 
NASKNAGEM IDKLTLTFN RTRQAVITR ELIEIISGA 
SAL
    CDS sequence
    • >TC009010 RA
      atgttgggt cgtttcggt agtttggtg cctttagtc 
acccaaaat gtgcaacaa caaaggggt atggccacc 
cttaagtcc atttcgatt cgcctcaag tccgtcaaa 
aacatccaa aagatcacc caatcaatg aagatggtg 
tcggccgct aaatactcc cgtgccgaa cgcgagctg 
aaacaaacg cgccccatc ggaagtgga gccacccag 
ttttacgag aaggccgaa atttcggcc cctgaggac 
cttccccag aagctttta atcgcaatg acttcagac 
agaggtctt tgtggagct gtccacacc agtgttgct 
cgtcttatt cgcaatgag ttgaataat gaccaacaa 
aatttgaaa gttgtgtgc gtcggagac aagtcgcga 
cttatcttg caacgcatg tatggcaaa aacattatc 
atggcatgc aacgaaatc gggcgtttg cccccaact 
ttcaccgat gcctcaaaa ttggccgat gctatcctc 
aactcccag tatgcgttt gggtcggga caaatcgtc 
tttaatcgg ttcaagtcg gtcgtgtcc tacagcacc 
cagaccttg cccgtcttc agcttggcc gcagttaag 
accgctgcc aagctcccg gtctacgac tccctcgac 
gatgaagtg atccagtcc tacttggaa ttctctctg 
gcttctctt ctgtattac gccatgaaa gagggcgcc 
tgcagcgag cagtcatcc cggatgacg gccatggac 
aacgccagc aaaaacgcc ggcgaaatg atcgacaaa 
ctcacccta acgttcaac cgtaccaga caggccgta 
atcaccaga gaactgatc gaaatcatc tctggtgct 
tcggccctt tag
    mRNA sequence
    • >TC009010 RA
      tatatctat cgttttgtt ggtgtaaga acttgaata 
tttattttt gtaaggcta aaaatttat aggactcca 
atcacaaat aaattgtag cagctccct ctactttta 
catgtcact ttttacgtc accaatttt ttataaact 
ttattgtca ggtgacata agagctatt attgcaaat 
ctccaaata gttcatttt ttagccact tcttgcagt 
ttctgaccc aggcgaagt cgcaagatc caatcatgt 
tgggtcgtt tcggtagtt tggtgcctt tagtcaccc 
aaaatgtgc aacaacaaa ggggtatgg ccaccctta 
agtccattt cgattcgcc tcaagtccg tcaaaaaca 
tccaaaaga tcacccaat caatgaaga tggtgtcgg 
ccgctaaat actcccgtg ccgaacgcg agctgaaac 
aaacgcgcc ccatcggaa gtggagcca cccagtttt 
acgagaagg ccgaaattt cggcccctg aggaccttc 
cccagaagc ttttaatcg caatgactt cagacagag 
gtctttgtg gagctgtcc acaccagtg ttgctcgtc 
ttattcgca atgagttga ataatgacc aacaaaatt 
tgaaagttg tgtgcgtcg gagacaagt cgcgactta 
tcttgcaac gcatgtatg gcaaaaaca ttatcatgg 
catgcaacg aaatcgggc gtttgcccc caactttca 
ccgatgcct caaaattgg ccgatgcta tcctcaact 
cccagtatg cgtttgggt cgggacaaa tcgtcttta 
atcggttca agtcggtcg tgtcctaca gcacccaga 
ccttgcccg tcttcagct tggccgcag ttaagaccg 
ctgccaagc tcccggtct acgactccc tcgacgatg 
aagtgatcc agtcctact tggaattct ctctggctt 
ctcttctgt attacgcca tgaaagagg gcgcctgca 
gcgagcagt catcccgga tgacggcca tggacaacg 
ccagcaaaa acgccggcg aaatgatcg acaaactca 
ccctaacgt tcaaccgta ccagacagg ccgtaatca 
ccagagaac tgatcgaaa tcatctctg gtgcttcgg 
ccctttagg taaataccc cgtaaaaac ttgatagta 
taatgctgt atgtaaggg tcgaacaga ataaaaacc 
acgtaagcc caaagtcac tggatcc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY