Status
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Gene information
General information
TC008992 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9103034..9107711
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008992
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01452

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    yolk potentially ectopicallypresent - Number of animals showing the phenotype: 4

    egg chamber mostlyshape irregular - Number of animals showing the phenotype: 4
    egg chamber mostlyposition irregular - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (19 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    Fertility
    egg production: number no/almost no eggs -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008992 RA
      MAEKEEFER AVDVVTEQE QEYAQKALN EFKKNNYAG 
      ALQFINKLD NRTNDFKVA HNKALVEYC KSDFRKNET 
      FQKNLTSIC NQFKIRLDK LDDVDQCIV HYNQAILLY 
      HQQQYTNAI YIMDRVYKF IEPMDDALA KQVSLFAIE 
      LQLCVRQSD KALSLINYL ENQLINGTP NIKLLDKTV 
      KEKDKKAPQ PLDPAMEEF KKKLIKYKI RCYLMNHNL 
      EIANKEISN LLKDKQNFQ ALFLAANLE YLKGNLIES 
      QKLLAAVPP DYLVYNDSG ESSTLMFYN NMGVIHHAM 
      GKPNLACHY FQLALKEDI ALTNNAKKD KGMDDKPLY 
      ALGGSKYHE LMYNLGVSL LHAKRPVQA FDCLIIAVR 
      RYHRNARLW MRLAECCIM ATKESNEVD FDIQKRQKE 
      IIVNVIGSQ DKQKVILTT NVSKDKKYS TESQSYAVP 
      VPSLEFASL CLRNASTLL PSDTVSSPI PLFLIPGGI 
      PPVPSCSPS PAPSSPLSP ESVMSLRNS ILAASAYVA 
      LCLGDYILA LEHAENLLQ QPRLSGAHK LLGHLYCAE 
      ALVLLDKIS EALEHLNPE NVKHINLEL PKGDENEEK 
      RIKTSPPLK WFPHNLPTA IATMQYNFA VAKTIRGQL 
      EDAIGLLKQ IWQMSSKCK VPGHIIMLL LYIELKLGR 
      PEPARKLVK QYLFQNRRK HVEIHHSLL RCS
    CDS sequence
    • >TC008992 RA
      atggctgag aaagaagag tttgaacga gctgtggac 
      gttgtaaca gagcaggag caggaatat gcacaaaag 
      gctttgaat gagtttaaa aaaaataat tacgccggt 
      gcgctccaa tttatcaat aaactggac aatagaact 
      aacgatttt aaagtggcc cacaataaa gctcttgtg 
      gaatattgc aaaagcgat tttaggaag aacgaaaca 
      tttcaaaag aatttaacc tcaatttgc aatcaattt 
      aagattcgt ctcgataaa ttagatgat gtggaccaa 
      tgcattgtt cattataat caggccatt ctgttatat 
      catcaacaa cagtacact aatgcaatt tacataatg 
      gatcgagtg tacaaattt attgaacca atggatgac 
      gcattagcc aaacaagtt agtcttttt gccattgag 
      ttacagcta tgtgtgcgt cagtctgat aaagcgctc 
      agtttaata aattatctg gaaaatcaa ttaatcaac 
      gggacgcca aacatcaaa ttgttggac aaaaccgtt 
      aaagagaaa gacaagaaa gcgccacag cccctagac 
      ccagcaatg gaagagttt aagaaaaaa ctaattaaa 
      tacaaaatc cgttgttac ctaatgaac cacaatctc 
      gaaatcgca aacaaagaa atcagcaat ttgctaaaa 
      gacaaacag aatttccaa gccttgttc ctggcagct 
      aatttagaa tacctcaaa ggaaacttg atagagtcc 
      cagaaactt cttgcggct gtaccaccc gattatctg 
      gtctacaac gacagcggc gaatcttca actttgatg 
      ttttacaac aatatgggt gtgattcat cacgcaatg 
      ggcaaaccc aacttggct tgccactac tttcagtta 
      gccctcaag gaggatata gctttaaca aacaatgca 
      aaaaaagac aaaggcatg gatgacaaa cccttgtat 
      gcactgggt ggctcaaaa taccacgaa ttgatgtat 
      aatttggga gtttctctt cttcatgcc aagaggccg 
      gttcaggcg tttgattgc ttgataatt gcagtgcgg 
      aggtaccac agaaacgcg aggttgtgg atgcgctta 
      gctgagtgt tgtattatg gccactaaa gagtcgaac 
      gaagttgac tttgatatt caaaagaga caaaaagag 
      ataatagtt aacgtcatt ggctcccaa gacaaacag 
      aaagttatc cttacgaca aacgtatca aaagacaaa 
      aaatacagt acggaaagt cagtcatac gccgttcct 
      gtcccctct ctcgaattt gcatcattg tgtttacgt 
      aacgcttcc actttactc ccttccgat acagtatcg 
      tcccctatt ccccttttt ttgatcccc gggggaatc 
      ccccctgtc ccatcttgt agtccaagc cccgcaccc 
      tcttcaccc ctatcaccc gaaagtgta atgagcctc 
      cgtaacagt attttggca gcaagtgcc tacgtagcc 
      ctctgtcta ggcgactat attttagca ctagaacat 
      gcggaaaac cttctgcaa caaccgaga ttatcgggt 
      gcccacaaa cttcttggt cacttgtac tgtgctgag 
      gcgctagtt ttgttagac aaaatttca gaagccttg 
      gaacattta aaccccgaa aatgtcaaa catattaat 
      ttagaattg ccaaaaggg gacgaaaat gaagagaaa 
      cggattaag acatcgccg cctctaaaa tggtttccg 
      cataatctg cccactgcg attgcaacg atgcaatat 
      aatttcgcg gtggctaag acaattaga ggccaactg 
      gaagacgcc atagggctt ttgaaacaa atctggcaa 
      atgagcagc aaatgtaaa gtgccagga catataatt 
      atgctactt ttgtacatt gaacttaaa ttaggacga 
      ccggaacca gccaggaaa ttagttaag cagtactta 
      ttccaaaat aggcgaaaa cacgtggaa atacatcac 
      tcgctcctt cgttgctcg taa
    mRNA sequence
    • >TC008992 RA
      gtgtttgca aaaaatcca ataaataca atggctgag 
      aaagaagag tttgaacga gctgtggac gttgtaaca 
      gagcaggag caggaatat gcacaaaag gctttgaat 
      gagtttaaa aaaaataat tacgccggt gcgctccaa 
      tttatcaat aaactggac aatagaact aacgatttt 
      aaagtggcc cacaataaa gctcttgtg gaatattgc 
      aaaagcgat tttaggaag aacgaaaca tttcaaaag 
      aatttaacc tcaatttgc aatcaattt aagattcgt 
      ctcgataaa ttagatgat gtggaccaa tgcattgtt 
      cattataat caggccatt ctgttatat catcaacaa 
      cagtacact aatgcaatt tacataatg gatcgagtg 
      tacaaattt attgaacca atggatgac gcattagcc 
      aaacaagtt agtcttttt gccattgag ttacagcta 
      tgtgtgcgt cagtctgat aaagcgctc agtttaata 
      aattatctg gaaaatcaa ttaatcaac gggacgcca 
      aacatcaaa ttgttggac aaaaccgtt aaagagaaa 
      gacaagaaa gcgccacag cccctagac ccagcaatg 
      gaagagttt aagaaaaaa ctaattaaa tacaaaatc 
      cgttgttac ctaatgaac cacaatctc gaaatcgca 
      aacaaagaa atcagcaat ttgctaaaa gacaaacag 
      aatttccaa gccttgttc ctggcagct aatttagaa 
      tacctcaaa ggaaacttg atagagtcc cagaaactt 
      cttgcggct gtaccaccc gattatctg gtctacaac 
      gacagcggc gaatcttca actttgatg ttttacaac 
      aatatgggt gtgattcat cacgcaatg ggcaaaccc 
      aacttggct tgccactac tttcagtta gccctcaag 
      gaggatata gctttaaca aacaatgca aaaaaagac 
      aaaggcatg gatgacaaa cccttgtat gcactgggt 
      ggctcaaaa taccacgaa ttgatgtat aatttggga 
      gtttctctt cttcatgcc aagaggccg gttcaggcg 
      tttgattgc ttgataatt gcagtgcgg aggtaccac 
      agaaacgcg aggttgtgg atgcgctta gctgagtgt 
      tgtattatg gccactaaa gagtcgaac gaagttgac 
      tttgatatt caaaagaga caaaaagag ataatagtt 
      aacgtcatt ggctcccaa gacaaacag aaagttatc 
      cttacgaca aacgtatca aaagacaaa aaatacagt 
      acggaaagt cagtcatac gccgttcct gtcccctct 
      ctcgaattt gcatcattg tgtttacgt aacgcttcc 
      actttactc ccttccgat acagtatcg tcccctatt 
      ccccttttt ttgatcccc gggggaatc ccccctgtc 
      ccatcttgt agtccaagc cccgcaccc tcttcaccc 
      ctatcaccc gaaagtgta atgagcctc cgtaacagt 
      attttggca gcaagtgcc tacgtagcc ctctgtcta 
      ggcgactat attttagca ctagaacat gcggaaaac 
      cttctgcaa caaccgaga ttatcgggt gcccacaaa 
      cttcttggt cacttgtac tgtgctgag gcgctagtt 
      ttgttagac aaaatttca gaagccttg gaacattta 
      aaccccgaa aatgtcaaa catattaat ttagaattg 
      ccaaaaggg gacgaaaat gaagagaaa cggattaag 
      acatcgccg cctctaaaa tggtttccg cataatctg 
      cccactgcg attgcaacg atgcaatat aatttcgcg 
      gtggctaag acaattaga ggccaactg gaagacgcc 
      atagggctt ttgaaacaa atctggcaa atgagcagc 
      aaatgtaaa gtgccagga catataatt atgctactt 
      ttgtacatt gaacttaaa ttaggacga ccggaacca 
      gccaggaaa ttagttaag cagtactta ttccaaaat 
      aggcgaaaa cacgtggaa atacatcac tcgctcctt 
      cgttgctcg taagtaatg tgctggaaa actgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY