Status
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Gene information
General information
TC008986 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9228885..9230707
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008986
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01451

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008986 RA
      MKTDNIAPG DVIWSNNDL LTDETCLPV IGNCQISSI 
      LSNLDSGDK NEVPTNVRA DNNDLKSSH ENNYVILKE 
      NSDKSDSVK KQSFKNGNN FENMLYFVC NLCPFLCTK 
      NSKITEHLE NAHKNKTVR KLPHLKCPA CPNVFFHKM 
      SLRSHLIHD HGVGNSDIN LIIQAVVYG ANKAKSSDS 
      KKVPFKIES RENSDGASP KSIDLSKVE TPTVKTENI 
      FNKHIISKL IGAKKTQTC VVPMCKVTL QSADNMNYH 
      ISCHKDSGF ACLVCSEFF MSWKQLTSH LWRLHKIDM 
      ELYSCDKCS YKSVSLAKL NNIHKLIHG DVRAYVCNV 
      CKKAFKNSK QLGNHKITH KKEFERLKF TCEICSKSF 
      SDKRQLKIH MNVVHEKIK PFLCNYCGY KGSSRSSLK 
      MHIRQHTGE KPFTCDSCS YATSDHNSL RRHKLRHTG 
      QKPYKCSYC SYACIQSST YKVHIKTKH PGQEKDLMF 
      TCQECQFRS VNKEMYVAH MITVHDQKP QS
    CDS sequence
    • >TC008986 RA
      atgaagact gataacatt gctccaggg gacgttatt 
      tggagcaat aacgacttg ttaaccgac gaaacatgt 
      ctccctgta atcggtaat tgccaaatc tcttcaatc 
      ctctcaaac ctcgactcc ggagacaag aacgaagtt 
      ccaacaaac gtgcgtgcc gacaataac gatttaaaa 
      tcaagccac gaaaacaat tacgtcata ctcaaagag 
      aacagtgat aagtccgat tcagtaaaa aaacaaagc 
      tttaagaat ggaaacaac tttgaaaac atgctgtac 
      ttcgtctgc aatttatgc ccgttcctg tgcacaaaa 
      aactcgaaa atcaccgaa cacctcgaa aacgcacac 
      aaaaacaaa acggttcgg aaactcccc cacttaaaa 
      tgcccagcg tgccctaac gtcttcttc cacaaaatg 
      tctctaagg tcccacttg atccacgac catggcgtc 
      ggcaactcc gacataaat ttgataatc caagcggtg 
      gtttatggc gcaaataaa gcaaaaagt tccgatagt 
      aagaaagta ccgtttaaa attgagtct cgagaaaac 
      agcgacggg gcttccccc aaaagcatc gacctatcg 
      aaagttgaa acaccaacg gtcaaaacc gaaaatatt 
      ttcaacaaa catatcatc agtaagtta attggggcg 
      aagaaaacc cagacctgc gtggtgcca atgtgcaaa 
      gtcactttg caaagtgca gacaatatg aactaccac 
      ataagttgc cacaaggac tcgggcttt gcgtgcctg 
      gtctgcagc gagtttttc atgtcctgg aagcagctc 
      acgagtcac ttgtggcga ctgcacaaa atcgacatg 
      gagctctac tcgtgcgat aagtgcagc tacaagtcg 
      gtcagtttg gccaaactg aacaacatc cacaagctg 
      attcacggc gacgtgcgg gcctacgtc tgcaacgtg 
      tgcaagaag gccttcaag aacagcaaa cagctcgga 
      aaccacaaa atcacccac aagaaggaa ttcgagcgt 
      ttgaagttc acctgcgag atctgctcg aagagcttt 
      tcggataaa agacagctc aaaatccac atgaacgtg 
      gtgcatgaa aaaatcaag ccgtttttg tgcaactac 
      tgcgggtac aagggctcg tcgcgcagc tctctgaaa 
      atgcacatc aggcaacac acgggggaa aaaccgttc 
      acttgtgat tcctgctcc tatgccacc tccgaccac 
      aactcgctc aggaggcac aagctgaga cacacgggc 
      caaaagccg tacaagtgt tcgtactgt tcctacgct 
      tgcatccag agtagtact tacaaagtg cacatcaag 
      accaaacat ccggggcag gagaaagac ctgatgttc 
      acgtgccag gagtgccag tttcggtcc gttaataaa 
      gaaatgtac gttgctcat atgatcacg gttcacgat 
      caaaaacca caaagttaa
    mRNA sequence
    • >TC008986 RA
      agaaatcaa ccaatcacg accacgcat ggttgacat 
      attttgaca agtgacatt tgtaggtta tcaactcgg 
      gtcattgtt gtctcggct acaaaaact cttccaaac 
      cagataaaa atatgtgta aatagttta aaaaagtga 
      ttgaatcac ttcaaacga acatgtcaa cgaaacggc 
      ccatttagt taatttttc ctttattta tttgcgaac 
      ggtgtaaat aggtcacaa tgaagactg ataacattg 
      ctccagggg acgttattt ggagcaata acgacttgt 
      taaccgacg aaacatgtc tccctgtaa tcggtaatt 
      gccaaatct cttcaatcc tctcaaacc tcgactccg 
      gagacaaga acgaagttc caacaaacg tgcgtgccg 
      acaataacg atttaaaat caagccacg aaaacaatt 
      acgtcatac tcaaagaga acagtgata agtccgatt 
      cagtaaaaa aacaaagct ttaagaatg gaaacaact 
      ttgaaaaca tgctgtact tcgtctgca atttatgcc 
      cgttcctgt gcacaaaaa actcgaaaa tcaccgaac 
      acctcgaaa acgcacaca aaaacaaaa cggttcgga 
      aactccccc acttaaaat gcccagcgt gccctaacg 
      tcttcttcc acaaaatgt ctctaaggt cccacttga 
      tccacgacc atggcgtcg gcaactccg acataaatt 
      tgataatcc aagcggtgg tttatggcg caaataaag 
      caaaaagtt ccgatagta agaaagtac cgtttaaaa 
      ttgagtctc gagaaaaca gcgacgggg cttccccca 
      aaagcatcg acctatcga aagttgaaa caccaacgg 
      tcaaaaccg aaaatattt tcaacaaac atatcatca 
      gtaagttaa ttggggcga agaaaaccc agacctgcg 
      tggtgccaa tgtgcaaag tcactttgc aaagtgcag 
      acaatatga actaccaca taagttgcc acaaggact 
      cgggctttg cgtgcctgg tctgcagcg agtttttca 
      tgtcctgga agcagctca cgagtcact tgtggcgac 
      tgcacaaaa tcgacatgg agctctact cgtgcgata 
      agtgcagct acaagtcgg tcagtttgg ccaaactga 
      acaacatcc acaagctga ttcacggcg acgtgcggg 
      cctacgtct gcaacgtgt gcaagaagg ccttcaaga 
      acagcaaac agctcggaa accacaaaa tcacccaca 
      agaaggaat tcgagcgtt tgaagttca cctgcgaga 
      tctgctcga agagctttt cggataaaa gacagctca 
      aaatccaca tgaacgtgg tgcatgaaa aaatcaagc 
      cgtttttgt gcaactact gcgggtaca agggctcgt 
      cgcgcagct ctctgaaaa tgcacatca ggcaacaca 
      cgggggaaa aaccgttca cttgtgatt cctgctcct 
      atgccacct ccgaccaca actcgctca ggaggcaca 
      agctgagac acacgggcc aaaagccgt acaagtgtt 
      cgtactgtt cctacgctt gcatccaga gtagtactt 
      acaaagtgc acatcaaga ccaaacatc cggggcagg 
      agaaagacc tgatgttca cgtgccagg agtgccagt 
      ttcggtccg ttaataaag aaatgtacg ttgctcata 
      tgatcacgg ttcacgatc aaaaaccac aaagttaaa 
      atagatttt atttatcta attaactta ataattttc 
      ccgaacgta a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY