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Gene information
General information
TC008973 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid49: 6162510..6166344
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008973
    iBeetle screen iB_01450
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    egg chamber mostlynot present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008973 RA
      MQRIFLIEI SDGTDGNPR SNSKPVLGP LETSHTMEI 
      AQLLLSLLH SWGLDNDLD RVCQVKLGL LRPMVPISF 
      GLLSKANQM SLFLPTWNS AILVDDESI PEDLSFGDE 
      KMSNMYPEL VKREHLTRI FTSHTHWEL STTLTSNHL 
      LAIIALSHT LMSMSNATF VPEQERNRK LHRQSTRVN 
      WSNTDEEHE EMYTQQQAQ IKQGWSLLA TLHCVLLPD 
      KVVEAGAKN FKRPQVEMM AKRWQHHCV EVREAAQTL 
      LLAELSRMG PKGRKALVD AWSQYLPLY TQTETINQQ 
      AVTSPNMNH VNSGQMPHS PDTAEEEFE EEEEEQIRK 
      PSSLSELKR KQSTAVILL GVIGAEFGQ DITGVDNNK 
      RRASEDRRK SSVVEGFGQ GNNNLARLT AMALSHLLL 
      APPSPKLPA HIPLRRAAI DLIGRGFTV WAPFLDISK 
      VLLGLLELC SDADRLVPS MTYGLPLTP QADSCRTAR 
      HALTLIATA RPAAFITTM AKEVARYNA MQQNAQTLN 
      INMNNNVLH KAKPEILRG VELLIDKMQ NEMSDLLVE 
      LMDIILHCL DPSQLKNKG LQDVFPAVC RFNQVSHCP 
      ATRRIAVGS NAGTLTLYE LRQGKCTTI NAHSSPVTA 
      AAFSPDGKF LVSYACGEN KLCFWQTST GMFGLGQSQ 
      TRCVKSYST APIADVARL NPMRLARLI WINNRTVTL 
      MLADGSETR FNV
    CDS sequence
    • >TC008973 RA
      atgcaaagg attttttta attgaaatt tcagacggc 
      acggacgga aacccgcgc tctaacagt aaaccggtg 
      ttgggcccc cttgagaca agccatacg atggagatc 
      gcccagctt ctcctgtct ctcctccac tcgtggggg 
      ctggacaac gacctagac agggtctgc caagtgaaa 
      ttaggttta ttaagaccc atggtgcct atttcattc 
      ggtcttttg tccaaagcg aatcaaatg tccttgttt 
      cttccgact tggaacagc gctattcta gtcgacgat 
      gaaagcatc cccgaagac ctgtcgttc ggggatgag 
      aaaatgagc aatatgtac ccggagtta gtcaagagg 
      gaacatttg acgagaatt ttcacttct cacacacat 
      tgggagttg agcacaacc cttactagc aatcatctt 
      ttggcgatt attgccctc agtcatact ttaatgtcg 
      atgagcaac gctacgttc gttccggaa caggaaagg 
      aaccggaag ttgcatcga cagtcaact cgagttaac 
      tggagcaat acagacgag gaacatgag gaaatgtac 
      acccaacaa caagcacaa atcaaacaa ggctggtcc 
      ttgttggcc actcttcat tgtgttctt ctaccagat 
      aaggtggta gaggcaggg gcgaagaac ttcaagcgg 
      ccacaggtc gaaatgatg gccaagagg tggcagcac 
      cactgcgtc gaagtgagg gaggccgcc cagacgctg 
      cttcttgcg gaactctcg aggatgggc ccgaagggc 
      cgtaaggcc ctagtggac gcctggtcg caatatttg 
      cccctgtac acgcaaacg gaaacaata aatcaacaa 
      gcggtcact tcgcccaat atgaaccac gtcaattcg 
      ggccaaatg ccacattcg ccggacaca gcggaggaa 
      gagttcgag gaggaggaa gaggaacag atccgcaag 
      ccgtcgagt ttgtccgaa ttgaagcga aaacagtcg 
      actgcagtt attttactg ggggtcata ggggccgag 
      ttcggtcag gacataacc ggtgtcgat aataacaaa 
      agacgagcg agtgaggat cggagaaaa agttcagtt 
      gttgagggt ttcggtcaa ggcaataat aatttagca 
      agactgact gcgatggcg ctgtcacat ttgttacta 
      gcgccgcct tcgcctaaa ctgcctgca cacattccg 
      ctaaggagg gcagctatt gatttaatt gggagaggg 
      tttacagtg tgggctcca tttttagat attagtaaa 
      gttttatta ggtttatta gaattgtgt tcggatgct 
      gatcggtta gtgccgagt atgacgtac ggtttgcct 
      ttgacgcct caagccgat tcgtgtcga acggcaagg 
      catgctctc actctcatt gcaacagca agacctgct 
      gcttttatt acgacaatg gccaaagaa gtcgctcga 
      tacaatgca atgcaacaa aatgcccaa actctaaac 
      ataaacatg aataacaac gtgttacac aaagccaag 
      ccggaaatt ttacgaggt gttgaacta ttaattgat 
      aagatgcaa aatgaaatg agtgatctc ttagttgaa 
      ttgatggat ataatcttg cactgtctc gatcctagt 
      caattgaaa aataagggc ctacaggac gtctttcct 
      gccgtttgt cggttcaac caggtttcg cattgtcct 
      gcaaccaga agaatcgcc gtcggttcg aacgcgggc 
      actttgacc ctgtacgag ctgagacag ggcaaatgc 
      acgacaatc aacgcccac tcgtcgccg gtgacggcg 
      gcggccttc agccccgac ggcaaattc ctagttagc 
      tacgcttgc ggcgagaac aagctttgc ttctggcaa 
      acgagcaca ggcatgttc ggcttgggc cagtcgcag 
      acgcgctgc gtcaagtcg tacagcacg gcccccatc 
      gccgacgtc gcgcgcctg aatcccatg aggctggca 
      agactgatc tggataaac aacaggacg gtgacgctg 
      atgctggcc gacggttcc gagacacga ttcaatgta 
      taa
    mRNA sequence
    • >TC008973 RA
      tttgggcaa ggtcaccac cgtacttaa atgttaata 
      tacgtgtta tgcaaagga tttttttaa ttgaaattt 
      cagacggca cggacggaa acccgcgct ctaacagta 
      aaccggtgt tgggccccc ttgagacaa gccatacga 
      tggagatcg cccagcttc tcctgtctc tcctccact 
      cgtgggggc tggacaacg acctagaca gggtctgcc 
      aagtgaaat taggtttat taagaccca tggtgccta 
      tttcattcg gtcttttgt ccaaagcga atcaaatgt 
      ccttgtttc ttccgactt ggaacagcg ctattctag 
      tcgacgatg aaagcatcc ccgaagacc tgtcgttcg 
      gggatgaga aaatgagca atatgtacc cggagttag 
      tcaagaggg aacatttga cgagaattt tcacttctc 
      acacacatt gggagttga gcacaaccc ttactagca 
      atcatcttt tggcgatta ttgccctca gtcatactt 
      taatgtcga tgagcaacg ctacgttcg ttccggaac 
      aggaaagga accggaagt tgcatcgac agtcaactc 
      gagttaact ggagcaata cagacgagg aacatgagg 
      aaatgtaca cccaacaac aagcacaaa tcaaacaag 
      gctggtcct tgttggcca ctcttcatt gtgttcttc 
      taccagata aggtggtag aggcagggg cgaagaact 
      tcaagcggc cacaggtcg aaatgatgg ccaagaggt 
      ggcagcacc actgcgtcg aagtgaggg aggccgccc 
      agacgctgc ttcttgcgg aactctcga ggatgggcc 
      cgaagggcc gtaaggccc tagtggacg cctggtcgc 
      aatatttgc ccctgtaca cgcaaacgg aaacaataa 
      atcaacaag cggtcactt cgcccaata tgaaccacg 
      tcaattcgg gccaaatgc cacattcgc cggacacag 
      cggaggaag agttcgagg aggaggaag aggaacaga 
      tccgcaagc cgtcgagtt tgtccgaat tgaagcgaa 
      aacagtcga ctgcagtta ttttactgg gggtcatag 
      gggccgagt tcggtcagg acataaccg gtgtcgata 
      ataacaaaa gacgagcga gtgaggatc ggagaaaaa 
      gttcagttg ttgagggtt tcggtcaag gcaataata 
      atttagcaa gactgactg cgatggcgc tgtcacatt 
      tgttactag cgccgcctt cgcctaaac tgcctgcac 
      acattccgc taaggaggg cagctattg atttaattg 
      ggagagggt ttacagtgt gggctccat ttttagata 
      ttagtaaag ttttattag gtttattag aattgtgtt 
      cggatgctg atcggttag tgccgagta tgacgtacg 
      gtttgcctt tgacgcctc aagccgatt cgtgtcgaa 
      cggcaaggc atgctctca ctctcattg caacagcaa 
      gacctgctg cttttatta cgacaatgg ccaaagaag 
      tcgctcgat acaatgcaa tgcaacaaa atgcccaaa 
      ctctaaaca taaacatga ataacaacg tgttacaca 
      aagccaagc cggaaattt tacgaggtg ttgaactat 
      taattgata agatgcaaa atgaaatga gtgatctct 
      tagttgaat tgatggata taatcttgc actgtctcg 
      atcctagtc aattgaaaa ataagggcc tacaggacg 
      tctttcctg ccgtttgtc ggttcaacc aggtttcgc 
      attgtcctg caaccagaa gaatcgccg tcggttcga 
      acgcgggca ctttgaccc tgtacgagc tgagacagg 
      gcaaatgca cgacaatca acgcccact cgtcgccgg 
      tgacggcgg cggccttca gccccgacg gcaaattcc 
      tagttagct acgcttgcg gcgagaaca agctttgct 
      tctggcaaa cgagcacag gcatgttcg gcttgggcc 
      agtcgcaga cgcgctgcg tcaagtcgt acagcacgg 
      cccccatcg ccgacgtcg cgcgcctga atcccatga 
      ggctggcaa gactgatct ggataaaca acaggacgg 
      tgacgctga tgctggccg acggttccg agacacgat 
      tcaatgtat aacaggcat gtaagaaaa ccgtggcct 
      ttcgactcg ttgttgttg ttctttcac gtctcttct 
      tatcctctc gtatgttag gatgtaagc catcattat 
      tcaattgca cggtaaatc cgattcctt acatatatg 
      tataaaaaa ttgatgagt tggttttgt gtcacaaga 
      caataattt cgattttgg ttttgttgt cgtcggttc 
      tcacagcca tgaaccgac ggctcgtgt attatatga 
      tagccttaa gatattcaa atgagtgtg cctcagcat 
      acagtctag ttaaaatca caaagtaag tattaatcg 
      atgtgttta tttacaccg tttagtatt ggaggattt 
      tgatggggg tagtggaca aaagaatca tcgacgtta 
      tccaataat tgttacatt tctgatgac attagtaca 
      attgagtcg cttcaatat gatatagct tgttgtatt 
      tattataaa tatgaagag gaagctcta tggagatat 
      aaagcagtc atacttttt gtaacgttt tatttatta 
      gtatatttc gcactttat ataattaaa aaagaatag 
      tgatatata ttatataga ggtaatttt attgtctat 
      gatgtacga tgttcataa actgtacat aaatgttat 
      ttattgtta ctttcagtc atttgagtt acaagtcaa 
      cggttaaac catctttaa tgtttactg gattttcga 
      aaaatttat ttatgccga cgttagata ttgttaata 
      attatgttg ttgagtttg ttgaaataa attttctat 
      caaagttgg tgctcagta atttgggaa ttcgtcaac 
      gacttgtag atgtcttga ggcctattt acggttttc 
      ctttcgtta aataacaat tccaaaaat gtgatctca 
      acaataact ttcgtttac atcactgtc tttaaatgt 
      aatgtcgtt gaacaattt atttttaag tatttgaac 
      tatttgatg tgcgtgtat tgtgaaaaa atacgttcc 
      ctatccagt tggcaccga aacttactg catttacgc 
      acccgcaca ctacgagag attacgata cgaatgcta 
      gtcagttag agaaagtga attgtaaat gtagaactt 
      ttcggtgtt tgtgttatg tatatatta taacataca 
      ttattagca atattctaa gtgttctgt gtacatgtg 
      tgtacatgg taattagtc aaaatatat tatctaatg 
      ataa