Status
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Gene information
General information
TC008961 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 12728723..12731319
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008961
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01446

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 1
    egg chamber shape rounded - Number of animals showing the phenotype: 3

    mature egg not present - Number of animals showing the phenotype: 3
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008961 RA
      MDHSRTIIH IDLDCFYAQ VEMNKNPSL RTVPLGIQQ 
      KNIVVTSNY TAREFGIKK CMLIPEAKK LCPSLVLVN 
      GEDLHDYRQ TSYKVTNLL QKYTPLVER LGLDENYLD 
      VTTLINEKL NQKSDLKLV GNVFGDTSD KCECGCTER 
      LIIATTVAQ EIRDVIKSE LNLTSCAGI AHNKLLAKI 
      AGARHKPNQ QTVVFPNSA LELILSLNA PNDIPGIGR 
      ALFEQLKCI GVETIEELQ NVDLKRLET VLGQDKAKL 
      VHELSLGID KSGVKPTGK PQSIGLEDS CRSISVETE 
      VKIKLEQLL NRLLLLVEE DGRVPKTIK LTVRKFNKD 
      NKMSHRETR QCNVNPSIF SSSQPNKEA KIMTTIMHL 
      FNRIIDVKK PYHITLLGL SFTKFQEPP KNTLASFLR 
      KDLEVQSVL SIENQNGVE DAESPVPST SKLAPTEGE 
      SPTPSPKKL KLTNLVSKK RCFEDYYED CESPSKLKV 
      ADLCLDSVS CPPDADKEV FKELPKEVQ KELWEDYKR 
      RRASEMNFC SSQVKKTKP NTLLNYFIR NN
    CDS sequence
    • >TC008961 RA
      atggatcat tcccgtact ataattcac atcgatttg 
      gactgtttt tacgcgcaa gtggaaatg aacaaaaac 
      cccagcctc cgaacggtc ccgctagga atacagcag 
      aaaaacatt gtcgtcact agcaattac acagcccgt 
      gaatttggc ataaaaaag tgcatgtta atccccgag 
      gcgaaaaaa ctgtgccct agcctcgtc ctagtcaac 
      ggcgaggat ttacacgac tatcgccag acctcgtac 
      aaagtaacc aatctgttg caaaagtac acccctctg 
      gtggaacgc ctaggtctt gatgagaat tatctggac 
      gtgacgacg ctaataaac gagaaattg aaccaaaaa 
      agtgatctc aagcttgtg ggaaatgtg tttggagac 
      acaagtgat aagtgtgag tgtggctgc acggagcgc 
      ctgatcatt gccacaaca gtggctcag gaaatccgc 
      gacgttatt aaatcggaa ctcaacctc acgtcctgt 
      gcaggaatc gcccataac aaactttta gcaaaaatt 
      gcaggagcg cgccacaaa ccaaaccaa caaactgtt 
      gttttccca aatagtgcg ttagaattg attcttagc 
      ttaaacgca ccgaacgat atcccggga attggcaga 
      gcgttgttt gaacaatta aaatgcata ggggttgaa 
      acaattgag gagctacaa aatgttgat ctcaagaga 
      cttgaaacc gttttaggg caagataaa gctaagtta 
      gtccatgag ttaagtttg ggtatcgat aaaagtggg 
      gtcaagccg acggggaag ccccaaagc attggttta 
      gaagacagt tgtaggtca atttctgta gaaaccgaa 
      gtgaaaata aaattagaa cagttgtta aaccgtctc 
      ttgcttctg gtggaagag gacgggcgt gtccccaaa 
      accataaaa ctaacagtc cggaagttc aacaaagac 
      aacaagatg agccaccga gagacgcgc caatgcaac 
      gtcaacccc tcgattttc tcctcaagc caaccaaac 
      aaagaagcc aaaatcatg accacgatc atgcacctt 
      ttcaaccga ataatcgac gtcaaaaaa ccctaccac 
      atcacatta ctcggcctt tctttcacg aagttccag 
      gagcccccg aaaaacacg ctcgcttcg ttcctgcgc 
      aaggacctt gaagtccag tcagtgctc agtatagag 
      aaccagaac ggtgtcgaa gacgccgaa agtcccgtc 
      ccttcaacc tcgaagttg gcaccaacg gaaggggaa 
      tcacccaca ccctcaccg aaaaaacta aagttgaca 
      aatctcgtg tcgaagaag cgctgtttt gaggactat 
      tacgaagac tgcgagagt ccctcaaag ttgaaggtt 
      gcggatttg tgcttggat tcggtgtcg tgcccccct 
      gatgccgac aaggaggtg ttcaaggag ctgccgaag 
      gaggtgcaa aaggagctg tgggaggat tacaagagg 
      aggagggcc agtgagatg aatttttgc agcagtcaa 
      gtgaagaaa accaagccc aacacgttg cttaattat 
      tttatccgg aataactga
    mRNA sequence
    • >TC008961 RA
      aaatcagta gaaataaac ataacctca ctaaagttg 
      caagagttc ttttatcag tatttaaaa acaatgtct 
      tttgagatg gatcattcc cgtactata attcacatc 
      gatttggac tgtttttac gcgcaagtg gaaatgaac 
      aaaaacccc agcctccga acggtcccg ctaggaata 
      cagcagaaa aacattgtc gtcactagc aattacaca 
      gcccgtgaa tttggcata aaaaagtgc atgttaatc 
      cccgaggcg aaaaaactg tgccctagc ctcgtccta 
      gtcaacggc gaggattta cacgactat cgccagacc 
      tcgtacaaa gtaaccaat ctgttgcaa aagtacacc 
      cctctggtg gaacgccta ggtcttgat gagaattat 
      ctggacgtg acgacgcta ataaacgag aaattgaac 
      caaaaaagt gatctcaag cttgtggga aatgtgttt 
      ggagacaca agtgataag tgtgagtgt ggctgcacg 
      gagcgcctg atcattgcc acaacagtg gctcaggaa 
      atccgcgac gttattaaa tcggaactc aacctcacg 
      tcctgtgca ggaatcgcc cataacaaa cttttagca 
      aaaattgca ggagcgcgc cacaaacca aaccaacaa 
      actgttgtt ttcccaaat agtgcgtta gaattgatt 
      cttagctta aacgcaccg aacgatatc ccgggaatt 
      ggcagagcg ttgtttgaa caattaaaa tgcataggg 
      gttgaaaca attgaggag ctacaaaat gttgatctc 
      aagagactt gaaaccgtt ttagggcaa gataaagct 
      aagttagtc catgagtta agtttgggt atcgataaa 
      agtggggtc aagccgacg gggaagccc caaagcatt 
      ggtttagaa gacagttgt aggtcaatt tctgtagaa 
      accgaagtg aaaataaaa ttagaacag ttgttaaac 
      cgtctcttg cttctggtg gaagaggac gggcgtgtc 
      cccaaaacc ataaaacta acagtccgg aagttcaac 
      aaagacaac aagatgagc caccgagag acgcgccaa 
      tgcaacgtc aacccctcg attttctcc tcaagccaa 
      ccaaacaaa gaagccaaa atcatgacc acgatcatg 
      caccttttc aaccgaata atcgacgtc aaaaaaccc 
      taccacatc acattactc ggcctttct ttcacgaag 
      ttccaggag cccccgaaa aacacgctc gcttcgttc 
      ctgcgcaag gaccttgaa gtccagtca gtgctcagt 
      atagagaac cagaacggt gtcgaagac gccgaaagt 
      cccgtccct tcaacctcg aagttggca ccaacggaa 
      ggggaatca cccacaccc tcaccgaaa aaactaaag 
      ttgacaaat ctcgtgtcg aagaagcgc tgttttgag 
      gactattac gaagactgc gagagtccc tcaaagttg 
      aaggttgcg gatttgtgc ttggattcg gtgtcgtgc 
      ccccctgat gccgacaag gaggtgttc aaggagctg 
      ccgaaggag gtgcaaaag gagctgtgg gaggattac 
      aagaggagg agggccagt gagatgaat ttttgcagc 
      agtcaagtg aagaaaacc aagcccaac acgttgctt 
      aattatttt atccggaat aactgatgg ttttgtaga 
      tttttttca ttaaatatg ttttaataa aaattggtt 
      ttttcttcc acccagtca ttcctaaat tccagattg 
      tatattttc gcgtggtgg taggatgtt aaaaataca 
      caattttta aaattatct gtattttat tattataaa 
      aaattcagt tttataata agtcatcaa taaaaaata 
      atcttcaac agatttata ttggtttaa aataatccg 
      taatccgct tgttgtttt tcaattgcc tgattgttt 
      tcgagtcgt aaattgaag ccttttggt tgaaacgtg 
      agtaaatgc cagcgcctg tttgcttat cagttttgt 
      atctgatga cttcacctt gaggcttta tgtctgacg 
      tttcaagaa agtcaagaa cactaatta catcgtcaa 
      ggtcggtgt agtctttgg tgtaaatta ttacagcat 
      gaaggggtt cagaatctg cagatgttg aaacttttg 
      cgtcccaaa gatctccag tttttacag cttccttta 
      ttttagtcc atatcccat ttacatctg cttttgata 
      aaaacaaca tccatttgc ccactttgc agtaaccat 
      tcacatttt ctaaactat atttgttgt tttttgccc 
      acagccagg gggcttcac cttttgtga cgtctgtcg 
      gattcagag tgcaatcat tctttattt cctttgatg 
      ctacatcac atttaaaga caaattttg cgtccatat 
      tttttctaa acctaaaaa actcgttca aaaattttt 
      atttacttg gaggtctta cgtttgcgt gctatttta 
      tgggtattt tactggtac tactgtcag taataccac 
      cacttctaa agtagtgtc acaactttg cccatgcac 
      ttgtgtatt attggttgc atcattaaa ttttcgaaa 
      aacac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY