General information | |||
TC008961 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 12728723..12731319 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC008961 |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
|
---|---|---|
GO ID | * {{ blurIdResult }} |
|
Evidence | {{ blurEvidenceResult }} |
|
Reference | * {{ blurReferenceResult }} |
|
Quotation | ||
Name | ||
Publish name | ||
{{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01446 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
egg chamber shape rounded - Number of animals showing the phenotype: 3
mature egg not present - Number of animals showing the phenotype: 3
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
- >TC008961 RA
MDHSRTIIH IDLDCFYAQ VEMNKNPSL RTVPLGIQQ KNIVVTSNY TAREFGIKK CMLIPEAKK LCPSLVLVN GEDLHDYRQ TSYKVTNLL QKYTPLVER LGLDENYLD VTTLINEKL NQKSDLKLV GNVFGDTSD KCECGCTER LIIATTVAQ EIRDVIKSE LNLTSCAGI AHNKLLAKI AGARHKPNQ QTVVFPNSA LELILSLNA PNDIPGIGR ALFEQLKCI GVETIEELQ NVDLKRLET VLGQDKAKL VHELSLGID KSGVKPTGK PQSIGLEDS CRSISVETE VKIKLEQLL NRLLLLVEE DGRVPKTIK LTVRKFNKD NKMSHRETR QCNVNPSIF SSSQPNKEA KIMTTIMHL FNRIIDVKK PYHITLLGL SFTKFQEPP KNTLASFLR KDLEVQSVL SIENQNGVE DAESPVPST SKLAPTEGE SPTPSPKKL KLTNLVSKK RCFEDYYED CESPSKLKV ADLCLDSVS CPPDADKEV FKELPKEVQ KELWEDYKR RRASEMNFC SSQVKKTKP NTLLNYFIR NN
- >TC008961 RA
atggatcat tcccgtact ataattcac atcgatttg gactgtttt tacgcgcaa gtggaaatg aacaaaaac cccagcctc cgaacggtc ccgctagga atacagcag aaaaacatt gtcgtcact agcaattac acagcccgt gaatttggc ataaaaaag tgcatgtta atccccgag gcgaaaaaa ctgtgccct agcctcgtc ctagtcaac ggcgaggat ttacacgac tatcgccag acctcgtac aaagtaacc aatctgttg caaaagtac acccctctg gtggaacgc ctaggtctt gatgagaat tatctggac gtgacgacg ctaataaac gagaaattg aaccaaaaa agtgatctc aagcttgtg ggaaatgtg tttggagac acaagtgat aagtgtgag tgtggctgc acggagcgc ctgatcatt gccacaaca gtggctcag gaaatccgc gacgttatt aaatcggaa ctcaacctc acgtcctgt gcaggaatc gcccataac aaactttta gcaaaaatt gcaggagcg cgccacaaa ccaaaccaa caaactgtt gttttccca aatagtgcg ttagaattg attcttagc ttaaacgca ccgaacgat atcccggga attggcaga gcgttgttt gaacaatta aaatgcata ggggttgaa acaattgag gagctacaa aatgttgat ctcaagaga cttgaaacc gttttaggg caagataaa gctaagtta gtccatgag ttaagtttg ggtatcgat aaaagtggg gtcaagccg acggggaag ccccaaagc attggttta gaagacagt tgtaggtca atttctgta gaaaccgaa gtgaaaata aaattagaa cagttgtta aaccgtctc ttgcttctg gtggaagag gacgggcgt gtccccaaa accataaaa ctaacagtc cggaagttc aacaaagac aacaagatg agccaccga gagacgcgc caatgcaac gtcaacccc tcgattttc tcctcaagc caaccaaac aaagaagcc aaaatcatg accacgatc atgcacctt ttcaaccga ataatcgac gtcaaaaaa ccctaccac atcacatta ctcggcctt tctttcacg aagttccag gagcccccg aaaaacacg ctcgcttcg ttcctgcgc aaggacctt gaagtccag tcagtgctc agtatagag aaccagaac ggtgtcgaa gacgccgaa agtcccgtc ccttcaacc tcgaagttg gcaccaacg gaaggggaa tcacccaca ccctcaccg aaaaaacta aagttgaca aatctcgtg tcgaagaag cgctgtttt gaggactat tacgaagac tgcgagagt ccctcaaag ttgaaggtt gcggatttg tgcttggat tcggtgtcg tgcccccct gatgccgac aaggaggtg ttcaaggag ctgccgaag gaggtgcaa aaggagctg tgggaggat tacaagagg aggagggcc agtgagatg aatttttgc agcagtcaa gtgaagaaa accaagccc aacacgttg cttaattat tttatccgg aataactga
- >TC008961 RA
aaatcagta gaaataaac ataacctca ctaaagttg caagagttc ttttatcag tatttaaaa acaatgtct tttgagatg gatcattcc cgtactata attcacatc gatttggac tgtttttac gcgcaagtg gaaatgaac aaaaacccc agcctccga acggtcccg ctaggaata cagcagaaa aacattgtc gtcactagc aattacaca gcccgtgaa tttggcata aaaaagtgc atgttaatc cccgaggcg aaaaaactg tgccctagc ctcgtccta gtcaacggc gaggattta cacgactat cgccagacc tcgtacaaa gtaaccaat ctgttgcaa aagtacacc cctctggtg gaacgccta ggtcttgat gagaattat ctggacgtg acgacgcta ataaacgag aaattgaac caaaaaagt gatctcaag cttgtggga aatgtgttt ggagacaca agtgataag tgtgagtgt ggctgcacg gagcgcctg atcattgcc acaacagtg gctcaggaa atccgcgac gttattaaa tcggaactc aacctcacg tcctgtgca ggaatcgcc cataacaaa cttttagca aaaattgca ggagcgcgc cacaaacca aaccaacaa actgttgtt ttcccaaat agtgcgtta gaattgatt cttagctta aacgcaccg aacgatatc ccgggaatt ggcagagcg ttgtttgaa caattaaaa tgcataggg gttgaaaca attgaggag ctacaaaat gttgatctc aagagactt gaaaccgtt ttagggcaa gataaagct aagttagtc catgagtta agtttgggt atcgataaa agtggggtc aagccgacg gggaagccc caaagcatt ggtttagaa gacagttgt aggtcaatt tctgtagaa accgaagtg aaaataaaa ttagaacag ttgttaaac cgtctcttg cttctggtg gaagaggac gggcgtgtc cccaaaacc ataaaacta acagtccgg aagttcaac aaagacaac aagatgagc caccgagag acgcgccaa tgcaacgtc aacccctcg attttctcc tcaagccaa ccaaacaaa gaagccaaa atcatgacc acgatcatg caccttttc aaccgaata atcgacgtc aaaaaaccc taccacatc acattactc ggcctttct ttcacgaag ttccaggag cccccgaaa aacacgctc gcttcgttc ctgcgcaag gaccttgaa gtccagtca gtgctcagt atagagaac cagaacggt gtcgaagac gccgaaagt cccgtccct tcaacctcg aagttggca ccaacggaa ggggaatca cccacaccc tcaccgaaa aaactaaag ttgacaaat ctcgtgtcg aagaagcgc tgttttgag gactattac gaagactgc gagagtccc tcaaagttg aaggttgcg gatttgtgc ttggattcg gtgtcgtgc ccccctgat gccgacaag gaggtgttc aaggagctg ccgaaggag gtgcaaaag gagctgtgg gaggattac aagaggagg agggccagt gagatgaat ttttgcagc agtcaagtg aagaaaacc aagcccaac acgttgctt aattatttt atccggaat aactgatgg ttttgtaga tttttttca ttaaatatg ttttaataa aaattggtt ttttcttcc acccagtca ttcctaaat tccagattg tatattttc gcgtggtgg taggatgtt aaaaataca caattttta aaattatct gtattttat tattataaa aaattcagt tttataata agtcatcaa taaaaaata atcttcaac agatttata ttggtttaa aataatccg taatccgct tgttgtttt tcaattgcc tgattgttt tcgagtcgt aaattgaag ccttttggt tgaaacgtg agtaaatgc cagcgcctg tttgcttat cagttttgt atctgatga cttcacctt gaggcttta tgtctgacg tttcaagaa agtcaagaa cactaatta catcgtcaa ggtcggtgt agtctttgg tgtaaatta ttacagcat gaaggggtt cagaatctg cagatgttg aaacttttg cgtcccaaa gatctccag tttttacag cttccttta ttttagtcc atatcccat ttacatctg cttttgata aaaacaaca tccatttgc ccactttgc agtaaccat tcacatttt ctaaactat atttgttgt tttttgccc acagccagg gggcttcac cttttgtga cgtctgtcg gattcagag tgcaatcat tctttattt cctttgatg ctacatcac atttaaaga caaattttg cgtccatat tttttctaa acctaaaaa actcgttca aaaattttt atttacttg gaggtctta cgtttgcgt gctatttta tgggtattt tactggtac tactgtcag taataccac cacttctaa agtagtgtc acaactttg cccatgcac ttgtgtatt attggttgc atcattaaa ttttcgaaa aacac
