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Gene information
General information
TC008957 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid49: 6036346..6039463
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008957
    iBeetle screen iB_01445
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008957 RA
      MAIFEQQTV KKVVLDDTE PLSCVIENW RNVNGHTEF 
      VIKVQRGPF SDKTWRVYK RYNDFYKLH AYLQTSGIS 
      LQLPPKKLI GNMDPEFIT ERQQGLQKY LNSVLMNPI 
      LVSSLPARS FVDPANYCQ PFGELALQH VSLALRGEV 
      GWEVVGPLE EMGWRLRKH YYHIKCKSQ PKDDLIGGW 
      TDYGPDKHL DDKDMHALF KSLNQIQHP FIHSIELCL 
      CTDTGGLVV RTNHKNGSL RDLLCGAKP RQSFLKKYG 
      NPKGHKPLE VSQVALYGR QILEALKFL MDKGLPYGH 
      LHTGNVIIE NDRVKLLDI ENGVLGVPS FYRPYFMQH 
      RKINTLQAI DVYCFGHTL YEMTFGAPL HESVADNIP 
      SCPSLLKSV LESILSSEA CKSGLPTIE SLLNHRFFS 
      SVAITLLPD DKAHLKIPN STKEHLKLA VAQMEERLK 
      EEQKMVRSQ KRLVKVQEM MSSEEEKKK QRHKLKQEQ 
      RQLAREQMR QKSKSEKDD VDRPDSVNS STATSIGTA 
      TPPSMTGSS VPSPPPPPP PPAPPSMSN GNGAPPLPE 
      LTKDRSALL GAICSFNKT SLRKTKVN
    CDS sequence
    • >TC008957 RA
      atggctata tttgagcaa cagacagtc aagaaagtt 
      gtactagac gatacagag cctctcagc tgtgttatc 
      gagaactgg agaaacgtc aatggacat acagaattt 
      gttataaaa gtgcaaagg gggcccttt tcagacaag 
      acctggcgt gtgtataag cgatacaat gatttctac 
      aagttgcac gcttacttg caaacctcg gggatttcc 
      ctccagttg ccccccaag aagctcatt gggaatatg 
      gacccggaa tttatcacc gagcggcaa caaggatta 
      cagaaatat ttaaacagc gtactgatg aatccaatt 
      ctagtctca tccctccca gcaagatct ttcgtagat 
      cctgccaac tattgtcaa ccttttggc gaactggcc 
      ttgcagcac gtttcactg gcgttgcgc ggggaagtg 
      ggttgggaa gttgtgggg ccgttggag gaaatgggc 
      tggagactc agaaagcac tactaccat ataaaatgt 
      aaatctcaa ccgaaagac gatttaata gggggctgg 
      accgactac ggtcctgat aaacacctc gatgacaag 
      gacatgcat gcgttattt aaaagtttg aatcagatt 
      cagcaccct ttcatacat tccattgaa ctgtgccta 
      tgcactgat actgggggc cttgttgtg aggaccaac 
      cacaagaac ggttctctg agagatttg ctatgcggg 
      gctaaaccg agacagtcg tttttaaaa aagtacggc 
      aatccgaaa ggacacaaa cctctggaa gttagtcaa 
      gtggcgctc tatggcagg cagattcta gaagcgctc 
      aagttttta atggataaa ggactacca tatggacat 
      ttgcacaca ggaaatgtg attatagaa aacgataga 
      gtcaaacta ttggacata gaaaacggt gttttgggg 
      gttccttcc ttttaccgc ccttatttt atgcaacac 
      cgcaaaata aatactctg caagcgatt gatgtctat 
      tgctttggg cacacgttg tatgagatg acttttggg 
      gccccactt cacgaaagt gtggccgat aacattccg 
      tcatgcccc tcgcttttg aaatccgta ttagaatcg 
      attttatcc tcagaggcg tgcaaatca ggactgccc 
      acaatcgaa tctctcctt aatcataga tttttttcg 
      tcagttgcg ataacgcta ctgccggac gataaggcg 
      catttaaaa ataccaaac tcgacgaag gaacacttg 
      aaactggcg gtcgcccaa atggaggaa cgattaaag 
      gaagaacag aagatggtc aggagtcag aagcgacta 
      gttaaagtg caggaaatg atgagttct gaggaagaa 
      aagaagaag cagagacac aagttgaag caagagcag 
      cgacagctt gctagagaa cagatgagg caaaagagc 
      aaatcggag aaagatgat gttgataga cctgatagt 
      gtgaacagc agtacagcg acttccatc gggacggca 
      acgccgccc tcgatgacc ggctctagt gttccgtcg 
      cccccaccg ccgccgccg cccccggca ccgccgtcg 
      atgtctaac gggaacggg gcgccccct cttccagaa 
      cttacaaag gatcggtct gctctattg ggcgcaatt 
      tgtagcttt aataaaacg tcgttgcgt aagaccaaa 
      gttaactaa
    mRNA sequence
    • >TC008957 RA
      actagttgg tagagtgtt acaaatgaa ggaatatat 
      cttcatttg agtgtcttt gagacttgg caacttggc 
      gcgatgact gacatttgg caaggttac gaagatgtt 
      tgacgttta aggaggcgt aatcgagta aatctcaaa 
      atctaatgg atttgcaac gagaaagtg agcaaatgt 
      acccatttc aatggctat atttgagca acagacagt 
      caagaaagt tgtactaga cgatacaga gcctctcag 
      ctgtgttat cgagaactg gagaaacgt caatggaca 
      tacagaatt tgttataaa agtgcaaag ggggccctt 
      ttcagacaa gacctggcg tgtgtataa gcgatacaa 
      tgatttcta caagttgca cgcttactt gcaaacctc 
      ggggatttc cctccagtt gccccccaa gaagctcat 
      tgggaatat ggacccgga atttatcac cgagcggca 
      acaaggatt acagaaata tttaaacag cgtactgat 
      gaatccaat tctagtctc atccctccc agcaagatc 
      tttcgtaga tcctgccaa ctattgtca accttttgg 
      cgaactggc cttgcagca cgtttcact ggcgttgcg 
      cggggaagt gggttggga agttgtggg gccgttgga 
      ggaaatggg ctggagact cagaaagca ctactacca 
      tataaaatg taaatctca accgaaaga cgatttaat 
      agggggctg gaccgacta cggtcctga taaacacct 
      cgatgacaa ggacatgca tgcgttatt taaaagttt 
      gaatcagat tcagcaccc tttcataca ttccattga 
      actgtgcct atgcactga tactggggg ccttgttgt 
      gaggaccaa ccacaagaa cggttctct gagagattt 
      gctatgcgg ggctaaacc gagacagtc gtttttaaa 
      aaagtacgg caatccgaa aggacacaa acctctgga 
      agttagtca agtggcgct ctatggcag gcagattct 
      agaagcgct caagttttt aatggataa aggactacc 
      atatggaca tttgcacac aggaaatgt gattataga 
      aaacgatag agtcaaact attggacat agaaaacgg 
      tgttttggg ggttccttc cttttaccg cccttattt 
      tatgcaaca ccgcaaaat aaatactct gcaagcgat 
      tgatgtcta ttgctttgg gcacacgtt gtatgagat 
      gacttttgg ggccccact tcacgaaag tgtggccga 
      taacattcc gtcatgccc ctcgctttt gaaatccgt 
      attagaatc gattttatc ctcagaggc gtgcaaatc 
      aggactgcc cacaatcga atctctcct taatcatag 
      atttttttc gtcagttgc gataacgct actgccgga 
      cgataaggc gcatttaaa aataccaaa ctcgacgaa 
      ggaacactt gaaactggc ggtcgccca aatggagga 
      acgattaaa ggaagaaca gaagatggt caggagtca 
      gaagcgact agttaaagt gcaggaaat gatgagttc 
      tgaggaaga aaagaagaa gcagagaca caagttgaa 
      gcaagagca gcgacagct tgctagaga acagatgag 
      gcaaaagag caaatcgga gaaagatga tgttgatag 
      acctgatag tgtgaacag cagtacagc gacttccat 
      cgggacggc aacgccgcc ctcgatgac cggctctag 
      tgttccgtc gcccccacc gccgccgcc gcccccggc 
      accgccgtc gatgtctaa cgggaacgg ggcgccccc 
      tcttccaga acttacaaa ggatcggtc tgctctatt 
      gggcgcaat ttgtagctt taataaaac gtcgttgcg 
      taagaccaa agttaacta aagttgtct ccaatatgt 
      tcgaattaa ttgatgttt tataatgtg atacgtaag 
      ctgtttgtt attttttag ggaagtcag gtaaactta 
      gtttgttgg agtaattag gtcaagaaa gttgaatgt 
      atttatttt tccaaatgc accaatatt attcataga 
      taaaaaggg tccacacac tgtataaaa tagcatagt 
      tttgtgttt ctccatgtt atgtatgtg ccttcccta 
      aattatttt tgtgtttcg ttgaaagag gaactgatt 
      gtgccaagg taatttgta tgacttcgt gtaaatgat 
      ggcatttaa tccattagc ccgataatt atgtaattg 
      ttaactagt caagtgttc ttgagttga atgtacagg 
      ttaagagga atttgcccc tttctataa ctactcaat 
      gtaacataa taggtaata aacgatagg attacgagc 
      tagtttttc attgaaaca ccgccttac accttaaac 
      aatttattt aacataaaa tataaacat taaactgga 
      ctaagcaat ttacaatgg atttaatca cgggatttg 
      tagttcttg atgtttacc aaaacccaa gctgggaca 
      atcatcata gacccggtg atgacaagc ccgtgaacc 
      accttctcg gcgaacgag attctgact ctagggggg 
      cccgaaata gcagacatt tgccgagtg gcttgcctc 
      acttgaggc gcaactctg accaaagta ccagtgaac 
      att