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Gene information
General information
TC008954 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 12616453..12626380
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008954
    iBeetle screen iB_01443

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008954 RA
      MNLKKRILL LVVALIFIA SLCVNSKQP PLPVPTEVE 
      GWARNVSRN VRDYISETA QAHIMPKNF CEEKGLLLV 
      FIHSKFDKF DARRAIRET WGQKRDNVT FYFLLGEDK 
      NSHHEVQLK LRDESQRFN DIVQERFVD SYNNLTLKS 
      ITMLKLFHL HCSDSYKYL LKIDDDVYL NIASALKEL 
      TNRSITTNV LLGHIYNVT NAIRNPASK WFVPYELYP 
      DEKYPPYLC GAAYIMSAD VAVKLYRVA LETPIFYIE 
      DVYITGMCA KKANVTLEN SGGFNCYYA KKYVCLYKQ 
      YFVYHYFEP RDIKKAHEL VAGKKCPLT GSWQEWFYS 
      LLY
    • >TC008954 RB
      MNLKKRILL LVVALIFIA SLCVNSKQP PLPVPTEVE 
      GWARNVSRN VRDYISETA QAHIMPKNF CEEKGLLLV 
      FIHSKFDKF DARRAIRET WGQKRDNVT FYFLLGEDK 
      NSHHEVQLK LRDESQRFN DIVQERFVD SYNNLTLKS 
      ITMLKLFHL HCSDSYKYL LKIDDDVYL NIASALKEL 
      TNRSITTNV LLGHIYNVT NAIRNPASK WFVPYELYP 
      DEKYPPYLC GAAYIMSAD VAVKLYRVA LETPIFYIE 
      DVYITGMCA KKANVTLEN SGGFNCYYA KKYVCLYKQ 
      YFVYHYFEP RDIKKAHEL VAGKKCPLT GSWQAILEE 
      ERALYNDII QERFIDSYN NLTLKSTFM LKVVNRYCK 
      NSFKYLMKA DDDVFVNLP RVLHMLSNR KTHENVILG 
      RLRRGWPIR DTYSKWYVP YEWYPEQEY PANVCGASY 
      IMSFDVARK LYDCALSTP LVHMEDIFL TGICGEKMN 
      VLRENNYMF TCNNRHFHF CYYKNYFTL HYYSAIDMV 
      NAWEMLHNH FCDYYLKFD VRKPWAFLF NFLF
    • >TC008954 RC
      MSIRRRFYI LCICFAIST GLSLLVYGY FFAGSSDLD 
      INMSRNMND YINQRLPPI IRPKKMCSE KKFLLVIVS 
      SRPKDVDLR KAIRETWGQ KHNNVTFYF IFGQSKKKA 
      KKYQAILEE ERALYNDII QERFIDSYN NLTLKSTFM 
      LKVVNRYCK NSFKYLMKA DDDVFVNLP RVLHMLSNR 
      KTHENVILG RLRRGWPIR DTYSKWYVP YEWYPEQEY 
      PANVCGASY IMSFDVARK LYDCALSTP LVHMEDIFL 
      TGICGEKMN VLRENNYMF TCNNRHFHF CYYKNYFTL 
      HYYSAIDMV NAWEMLHNH FCDYYLKFD VRKPWAFLF 
      NFLF
    CDS sequence
    • >TC008954 RA
      atgaactta aagaaaaga atactgctt ttggtcgtc 
      gctctgata ttcattgct tcgctctgt gttaacagc 
      aaacaaccg ccgctccca gtcccaaca gaggtggag 
      ggttgggcc cgaaatgtt tcgcggaat gtcagggac 
      tacatcagc gaaacggcg caagcacac attatgcca 
      aaaaatttc tgtgaggaa aaggggctc ctcctagtg 
      ttcatccac tcgaagttc gataagttc gatgcccgg 
      agggccata cgggagacg tggggccaa aaacgtgac 
      aatgtaacg ttttatttt ttgcttggg gaagacaaa 
      aatagtcac catgaagta caactcaag ttgagagac 
      gagagccag agatttaat gatatagtt caggaacgc 
      ttcgtagat tcgtacaat aacttaact ctaaagtct 
      ataaccatg ttgaagttg tttcacctg cattgttca 
      gattcttac aaatattta ttaaaaatc gacgacgat 
      gtgtatttg aacatcgcc agcgcactc aaagaactc 
      acgaaccgc agtattacc acgaacgtg ttgctgggc 
      cacatttac aacgtcacg aatgcgatt cggaaccca 
      gccagcaaa tggtttgtg ccttatgaa ctgtacccg 
      gacgagaaa tacccgccg tatttgtgc ggggcggcg 
      tacattatg tcggccgat gtcgcagtg aagctgtac 
      cgtgtggct ttggaaacg ccgattttc tacatcgag 
      gatgtttac ataacggga atgtgtgcg aagaaagcc 
      aatgtgaca ttggaaaac agtggcggt tttaattgc 
      tattacgcg aaaaaatac gtatgcctt tataaacaa 
      tacttcgtt tatcattat ttcgaacct agggacatt 
      aaaaaggcg catgagctc gtggctggc aagaagtgc 
      cctttgacc ggatcgtgg caagaatgg ttctactct 
      cttttatat tga
    • >TC008954 RB
      atgaactta aagaaaaga atactgctt ttggtcgtc 
      gctctgata ttcattgct tcgctctgt gttaacagc 
      aaacaaccg ccgctccca gtcccaaca gaggtggag 
      ggttgggcc cgaaatgtt tcgcggaat gtcagggac 
      tacatcagc gaaacggcg caagcacac attatgcca 
      aaaaatttc tgtgaggaa aaggggctc ctcctagtg 
      ttcatccac tcgaagttc gataagttc gatgcccgg 
      agggccata cgggagacg tggggccaa aaacgtgac 
      aatgtaacg ttttatttt ttgcttggg gaagacaaa 
      aatagtcac catgaagta caactcaag ttgagagac 
      gagagccag agatttaat gatatagtt caggaacgc 
      ttcgtagat tcgtacaat aacttaact ctaaagtct 
      ataaccatg ttgaagttg tttcacctg cattgttca 
      gattcttac aaatattta ttaaaaatc gacgacgat 
      gtgtatttg aacatcgcc agcgcactc aaagaactc 
      acgaaccgc agtattacc acgaacgtg ttgctgggc 
      cacatttac aacgtcacg aatgcgatt cggaaccca 
      gccagcaaa tggtttgtg ccttatgaa ctgtacccg 
      gacgagaaa tacccgccg tatttgtgc ggggcggcg 
      tacattatg tcggccgat gtcgcagtg aagctgtac 
      cgtgtggct ttggaaacg ccgattttc tacatcgag 
      gatgtttac ataacggga atgtgtgcg aagaaagcc 
      aatgtgaca ttggaaaac agtggcggt tttaattgc 
      tattacgcg aaaaaatac gtatgcctt tataaacaa 
      tacttcgtt tatcattat ttcgaacct agggacatt 
      aaaaaggcg catgagctc gtggctggc aagaagtgc 
      cctttgacc ggatcgtgg caagctata ctcgaggaa 
      gaacgagcc ctttataac gacatcatc caagaacgc 
      ttcatcgac tcttacaac aatctaaca ctaaaatcc 
      acctttatg ttgaaagtc gtcaatcgc tattgcaaa 
      aattcgttc aaatatttg atgaaagcc gatgacgac 
      gtttttgtg aacctcccg cgcgttttg cacatgctt 
      tcaaaccgg aagacgcat gagaacgtg attttgggg 
      aggctgagg cgtggctgg cccatcaga gacacctac 
      agcaaatgg tatgtgccc tacgagtgg taccctgaa 
      caggaatac ccagccaat gtctgtggc gctagctac 
      ataatgtcg ttcgatgtg gccagaaaa ctgtacgat 
      tgtgccctg agcacgcct ttggtgcac atggaggac 
      atttttttg actggaatt tgcggcgaa aaaatgaac 
      gttttgcga gaaaataac tacatgttc acgtgcaat 
      aataggcac ttccatttc tgctattac aagaattat 
      tttactctg cactattac agtgcgata gacatggtg 
      aatgcgtgg gaaatgttg cacaaccac ttttgcgat 
      tattatttg aagtttgat gttagaaag ccctgggca 
      ttccttttt aatttcttg ttctaa
    • >TC008954 RC
      atgagtata agacggagg ttttacatt ttgtgcatt 
      tgtttcgct atttctaca ggacttagt ttgctggtc 
      tatggctac ttcttcgct ggttcgtct gatttagac 
      ataaacatg tcacgaaac atgaacgat tacataaat 
      cagagactc ccaccgatt attcgcccc aaaaaaatg 
      tgttccgaa aaaaagttc ctgctagta attgtgtca 
      tcgcgaccc aaagatgtg gatcttcgc aaagctatc 
      agagaaacg tggggacaa aaacataac aacgtcacg 
      ttttatttc atttttggc cagtctaag aaaaaggcg 
      aaaaagtac caagctata ctcgaggaa gaacgagcc 
      ctttataac gacatcatc caagaacgc ttcatcgac 
      tcttacaac aatctaaca ctaaaatcc acctttatg 
      ttgaaagtc gtcaatcgc tattgcaaa aattcgttc 
      aaatatttg atgaaagcc gatgacgac gtttttgtg 
      aacctcccg cgcgttttg cacatgctt tcaaaccgg 
      aagacgcat gagaacgtg attttgggg aggctgagg 
      cgtggctgg cccatcaga gacacctac agcaaatgg 
      tatgtgccc tacgagtgg taccctgaa caggaatac 
      ccagccaat gtctgtggc gctagctac ataatgtcg 
      ttcgatgtg gccagaaaa ctgtacgat tgtgccctg 
      agcacgcct ttggtgcac atggaggac atttttttg 
      actggaatt tgcggcgaa aaaatgaac gttttgcga 
      gaaaataac tacatgttc acgtgcaat aataggcac 
      ttccatttc tgctattac aagaattat tttactctg 
      cactattac agtgcgata gacatggtg aatgcgtgg 
      gaaatgttg cacaaccac ttttgcgat tattatttg 
      aagtttgat gttagaaag ccctgggca ttccttttt 
      aatttcttg ttctaa
    mRNA sequence
    • >TC008954 RA
      aaagccaga atgattagg gagcacaat gattctatt 
      tatagttta cgagcaata aaagtgtgt tttatattt 
      ggcgcccct agtcacgcc actaccaac ccaacaaac 
      ctgcactca ccactagtt gacccacgt tttaaaagt 
      tgtttgggt gctttctgt tgattgtgt acttaaaaa 
      ccgcttatc acaactcaa ccgcaatct ttaatcgtg 
      ataacgcac cgatatcat cttcattac cacaaagac 
      tctcccgga ggtgataac aagcatcga gtggcttcc 
      tgtcactgc cacccatat cagatttta ggtgtttta 
      tggggccga taacgattt ccgagtagg attggaatg 
      gtcggaaac acttgatgc tgagtttgc tctccgctc 
      agtctttcc ccgtaatcg gcatgccct tgcctcagt 
      catcagata ttttatctc tttaactac agataacag 
      tactgcact taagtgtag catgtgttg attgagcga 
      taggcgcga atcgatggt gaacgcgac aactcttcc 
      atcggttca acttcagtg gaataaatg aacttaaag 
      aaaagaata ctgcttttg gtcgtcgct ctgatattc 
      attgcttcg ctctgtgtt aacagcaaa caaccgccg 
      ctcccagtc ccaacagag gtggagggt tgggcccga 
      aatgtttcg cggaatgtc agggactac atcagcgaa 
      acggcgcaa gcacacatt atgccaaaa aatttctgt 
      gaggaaaag gggctcctc ctagtgttc atccactcg 
      aagttcgat aagttcgat gcccggagg gccatacgg 
      gagacgtgg ggccaaaaa cgtgacaat gtaacgttt 
      tattttttg cttggggaa gacaaaaat agtcaccat 
      gaagtacaa ctcaagttg agagacgag agccagaga 
      tttaatgat atagttcag gaacgcttc gtagattcg 
      tacaataac ttaactcta aagtctata accatgttg 
      aagttgttt cacctgcat tgttcagat tcttacaaa 
      tatttatta aaaatcgac gacgatgtg tatttgaac 
      atcgccagc gcactcaaa gaactcacg aaccgcagt 
      attaccacg aacgtgttg ctgggccac atttacaac 
      gtcacgaat gcgattcgg aacccagcc agcaaatgg 
      tttgtgcct tatgaactg tacccggac gagaaatac 
      ccgccgtat ttgtgcggg gcggcgtac attatgtcg 
      gccgatgtc gcagtgaag ctgtaccgt gtggctttg 
      gaaacgccg attttctac atcgaggat gtttacata 
      acgggaatg tgtgcgaag aaagccaat gtgacattg 
      gaaaacagt ggcggtttt aattgctat tacgcgaaa 
      aaatacgta tgcctttat aaacaatac ttcgtttat 
      cattatttc gaacctagg gacattaaa aaggcgcat 
      gagctcgtg gctggcaag aagtgccct ttgaccgga 
      tcgtggcaa gaatggttc tactctctt ttatattga 
      accaagatg atctgagaa ggtacttaa atatgtgat 
      ttagagtat ttttttaat ctttttgtt tattcgttt 
      caagaataa aagattttt gcgtat
    • >TC008954 RB
      aaagccaga atgattagg gagcacaat gattctatt 
      tatagttta cgagcaata aaagtgtgt tttatattt 
      ggcgcccct agtcacgcc actaccaac ccaacaaac 
      ctgcactca ccactagtt gacccacgt tttaaaagt 
      tgtttgggt gctttctgt tgattgtgt acttaaaaa 
      ccgcttatc acaactcaa ccgcaatct ttaatcgtg 
      ataacgcac cgatatcat cttcattac cacaaagac 
      tctcccgga ggtgataac aagcatcga gtggcttcc 
      tgtcactgc cacccatat cagatttta ggtgtttta 
      tggggccga taacgattt ccgagtagg attggaatg 
      gtcggaaac acttgatgc tgagtttgc tctccgctc 
      agtctttcc ccgtaatcg gcatgccct tgcctcagt 
      catcagata ttttatctc tttaactac agataacag 
      tactgcact taagtgtag catgtgttg attgagcga 
      taggcgcga atcgatggt gaacgcgac aactcttcc 
      atcggttca acttcagtg gaataaatg aacttaaag 
      aaaagaata ctgcttttg gtcgtcgct ctgatattc 
      attgcttcg ctctgtgtt aacagcaaa caaccgccg 
      ctcccagtc ccaacagag gtggagggt tgggcccga 
      aatgtttcg cggaatgtc agggactac atcagcgaa 
      acggcgcaa gcacacatt atgccaaaa aatttctgt 
      gaggaaaag gggctcctc ctagtgttc atccactcg 
      aagttcgat aagttcgat gcccggagg gccatacgg 
      gagacgtgg ggccaaaaa cgtgacaat gtaacgttt 
      tattttttg cttggggaa gacaaaaat agtcaccat 
      gaagtacaa ctcaagttg agagacgag agccagaga 
      tttaatgat atagttcag gaacgcttc gtagattcg 
      tacaataac ttaactcta aagtctata accatgttg 
      aagttgttt cacctgcat tgttcagat tcttacaaa 
      tatttatta aaaatcgac gacgatgtg tatttgaac 
      atcgccagc gcactcaaa gaactcacg aaccgcagt 
      attaccacg aacgtgttg ctgggccac atttacaac 
      gtcacgaat gcgattcgg aacccagcc agcaaatgg 
      tttgtgcct tatgaactg tacccggac gagaaatac 
      ccgccgtat ttgtgcggg gcggcgtac attatgtcg 
      gccgatgtc gcagtgaag ctgtaccgt gtggctttg 
      gaaacgccg attttctac atcgaggat gtttacata 
      acgggaatg tgtgcgaag aaagccaat gtgacattg 
      gaaaacagt ggcggtttt aattgctat tacgcgaaa 
      aaatacgta tgcctttat aaacaatac ttcgtttat 
      cattatttc gaacctagg gacattaaa aaggcgcat 
      gagctcgtg gctggcaag aagtgccct ttgaccgga 
      tcgtggcaa gctatactc gaggaagaa cgagccctt 
      tataacgac atcatccaa gaacgcttc atcgactct 
      tacaacaat ctaacacta aaatccacc tttatgttg 
      aaagtcgtc aatcgctat tgcaaaaat tcgttcaaa 
      tatttgatg aaagccgat gacgacgtt tttgtgaac 
      ctcccgcgc gttttgcac atgctttca aaccggaag 
      acgcatgag aacgtgatt ttggggagg ctgaggcgt 
      ggctggccc atcagagac acctacagc aaatggtat 
      gtgccctac gagtggtac cctgaacag gaataccca 
      gccaatgtc tgtggcgct agctacata atgtcgttc 
      gatgtggcc agaaaactg tacgattgt gccctgagc 
      acgcctttg gtgcacatg gaggacatt tttttgact 
      ggaatttgc ggcgaaaaa atgaacgtt ttgcgagaa 
      aataactac atgttcacg tgcaataat aggcacttc 
      catttctgc tattacaag aattatttt actctgcac 
      tattacagt gcgatagac atggtgaat gcgtgggaa 
      atgttgcac aaccacttt tgcgattat tatttgaag 
      tttgatgtt agaaagccc tgggcattc ctttttaat 
      ttcttgttc taattttgg agcgtggtt gtaattttt 
      taaacacac ttcaaataa aaaa
    • >TC008954 RC
      caggtaaga aagcaatga ttctcattt accgcaaat 
      gagcccaaa aaggaattt gctttaagt cttgtttta 
      acaactgtt tcacttttc acatattta cattgtaga 
      agtaataaa ttaaccttg tgtttttta agtaaaatt 
      cgttccaaa ttgaagttg tgaaaacct gcgatgagt 
      ataagacgg aggttttac attttgtgc atttgtttc 
      gctatttct acaggactt agtttgctg gtctatggc 
      tacttcttc gctggttcg tctgattta gacataaac 
      atgtcacga aacatgaac gattacata aatcagaga 
      ctcccaccg attattcgc cccaaaaaa atgtgttcc 
      gaaaaaaag ttcctgcta gtaattgtg tcatcgcga 
      cccaaagat gtggatctt cgcaaagct atcagagaa 
      acgtgggga caaaaacat aacaacgtc acgttttat 
      ttcattttt ggccagtct aagaaaaag gcgaaaaag 
      taccaagct atactcgag gaagaacga gccctttat 
      aacgacatc atccaagaa cgcttcatc gactcttac 
      aacaatcta acactaaaa tccaccttt atgttgaaa 
      gtcgtcaat cgctattgc aaaaattcg ttcaaatat 
      ttgatgaaa gccgatgac gacgttttt gtgaacctc 
      ccgcgcgtt ttgcacatg ctttcaaac cggaagacg 
      catgagaac gtgattttg gggaggctg aggcgtggc 
      tggcccatc agagacacc tacagcaaa tggtatgtg 
      ccctacgag tggtaccct gaacaggaa tacccagcc 
      aatgtctgt ggcgctagc tacataatg tcgttcgat 
      gtggccaga aaactgtac gattgtgcc ctgagcacg 
      cctttggtg cacatggag gacattttt ttgactgga 
      atttgcggc gaaaaaatg aacgttttg cgagaaaat 
      aactacatg ttcacgtgc aataatagg cacttccat 
      ttctgctat tacaagaat tattttact ctgcactat 
      tacagtgcg atagacatg gtgaatgcg tgggaaatg 
      ttgcacaac cacttttgc gattattat ttgaagttt 
      gatgttaga aagccctgg gcattcctt tttaatttc 
      ttgttctaa ttttggagc gtggttgta attttttaa 
      acacacttc aaataaaaa a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY