Status
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Gene information
General information
TC033742
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008947,TC008938
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 12386573..12484214
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033742
    iBeetle screen iB_01441

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033742 RA
      MSVVKRVQQ WATFARIWH VYDAAWQDP 
      FQSAQLIKK YLMGLYKPI YHPMNDCGD 
      HVIVINTNE IAMPEEEWK KRAYFHHTG 
      YPGGASWTL AWELHSKDP KLILKKAVY 
      KSMAGNLQR RHTMERLHL FADENVPAD 
      LMKNVSNQI RQLRPMPKR LDHYTEEEV 
      KSFPKLIDY PKDYILK
    • >TC033742 RB
      MTLQGVKRK VVTVSDFRF TGRLLGKGN FARVEEAVH 
      NVLNVKVAV KIIDIREIK EKYVVKNLH REARIMSTL 
      NHPCIASLF QTMQLADNV YYLVTELVS GGDLCSFII 
      GQRYGKLEE RPARVYARQ FASALAHMH SYGIVHRDL 
      KMENVMLNS SQTQIKIVD FGLSNVWTS ENPLRTHCG 
      SPEYAAPEL FIAGREYGP EVDLWSLGI IFYGMVVGQ 
      LPFVGGRKN QVSSQERRK RLVAQINKG LGATQRQAL 
      APLSPEFRT MLNKLLAPE PVKRITTKE LITHPWITE 
      KGRKCVQMH PFKELDNDT RAQMIRKLG ELFQVDATT 
      VTTELFKNP YGRLGGINN ILYYKHINE QFNPELIAT 
      TIQNSAPET VPVKPTTKP KSASLAKSQ HTLYNQCIV 
      LLNKVSKQQ TEARSKTAN PALTLTKNI AKLSRSKAQ 
      SAKPTYSKT NCVVINRKT REDAAKTPN SSNVREAPD 
      GRPEAASCH HGDDTSRHK TDKTQQQWA TFARIWHVY 
      DAAWQDPFQ SAQLIKKYL MGLYKPIYH PMNDCGDHV 
      IVINTNEIA MPEEEWKKR AYFHHTGYP GGASWTLAW 
      ELHSKDPKL ILKKAVYKS MAGNLQRRH TMERLHLFA 
      DENVPADLM KNVSNQIRQ LRPMPKRLD HYTEEEVKS 
      FPKLIDYPK DYILK
    • >TC033742 RC
      MTLQGVKRK VVTVSDFRF TGRLLGKGN FARVEEAVH 
      NVLNVKVAV KIIDIREIK EKYVVKNLH REARIMSTL 
      NHPCIASLF QTMQLADNV YYLVTELVS GGDLCSFII 
      GQRYGKLEE RPARVYARQ FASALAHMH SYGIVHRDL 
      KMENVMLNS SQTQIKIVD FGLSNVWTS ENPLRTHCG 
      SPEYAAPEL FIAGREYGP EVDLWSLGI IFYGMVVGQ 
      LPFVGGRKN QVSSQERRK RLVAQINKG LGATQRQAL 
      APLSPEFRT MLNKLLAPE PVKRITTKE LITHPWITE 
      KGRKCVQMH PFKELDNDT RAQMIRKLG ELFQVDATT 
      VTTELFKNP YGRLGGINN ILYYKHINE QFNPELIAT 
      TIQNSAPET VPVKPTTKP KSASLAKSQ HTLYNQCIV 
      LLNKVSKQQ TEARSKTAN PALTLTKNI AKLSRSKAQ 
      SAKPTYSKT NCVVINRKT REDAAKTPN SSNVREAPD 
      GRPEAASCH HGDDTSRHK TDKTQQCVV SNATDVQVK 
      QPRISTPPD MHKSSRNGR RDTLKKRTL QCCFAKAAV 
      QGSSS
    CDS sequence
    • >TC033742 RA
      atgtctgtg gtaaaaagg gttcagcaa tgggccacc 
      tttgcaagg atatggcat gtgtacgat gccgcttgg 
      caggaccca tttcagtcc gcgcaactg ataaaaaag 
      tatttaatg ggcttatat aaaccaatt taccaccca 
      atgaatgat tgtggtgac catgtaatt gttataaac 
      actaatgag atcgcgatg cctgaagaa gagtggaag 
      aaacgggct tattttcac cacacgggt tatcctggg 
      ggtgcgtct tggacccta gcttgggaa ttacacagc 
      aaggacccg aaattaatt ctcaaaaaa gcagtttat 
      aaaagcatg gccggcaat ttgcagaga cggcacaca 
      atggagagg ctgcatctt tttgctgat gaaaatgta 
      ccggcagat ttgatgaaa aacgtttct aatcaaata 
      agacaactt agaccaatg ccaaagaga ctagaccat 
      tatactgaa gaagaagtt aaatcattt ccaaaactt 
      atcgattat ccgaaagat tacattctg aaataa
    • >TC033742 RB
      atgacttta caaggtgtc aagcgaaaa gttgtcacc 
      gtgagcgac ttccggttt acgggacgc ctgctggga 
      aagggaaac tttgcccga gtcgaagaa gccgtccac 
      aacgtcctc aatgtcaag gtggcggtg aagataatc 
      gatatcaga gaaatcaag gaaaagtac gtcgtaaaa 
      aacttgcac agagaagcc cgcataatg tcgacattg 
      aatcacccc tgtatagcg tctcttttc cagaccatg 
      cagttggct gataacgtg tactatctg gttaccgag 
      cttgtttcc ggaggtgat ctttgttct ttcatcatt 
      ggccaacgt tatgggaag ttggaagaa cgcccagca 
      agagtttat gcaagacaa ttcgcttct gctttggct 
      catatgcac agttatgga atcgtccac agagacctc 
      aagatggag aacgtgatg ctgaactcg tcgcagacg 
      cagataaaa atcgtggat ttcggttta agcaacgtc 
      tggaccagc gaaaatcct ctgcgaacc cactgcggg 
      tccccggag tacgccgct ccggagctt ttcattgcc 
      gggagagag tacggaccg gaagtcgac ctctggagt 
      ctggggatc attttctac ggcatggtc gtggggcag 
      ctgcccttt gtcgggggg cgaaaaaat caagtgtcg 
      tcgcaggaa cgcagaaag cgactcgtg gcgcaaatc 
      aacaagggc ctgggggcc acgcaacgc caagctctg 
      gccccccta tcgcccgaa ttccgcacg atgttaaac 
      aaactgcta gcacctgaa ccggtgaaa aggatcacc 
      acgaaggag ctcatcaca catccgtgg atcacggaa 
      aagggccgg aaatgcgtg caaatgcac ccgtttaaa 
      gagctggat aacgacacc cgagcccaa atgattaga 
      aagttgggc gagctgttc caagtggac gcgaccacg 
      gttacgacg gaattgttc aaaaacccg tacgggaga 
      cttggcgga ataaacaac attttgtac tacaaacac 
      ataaacgaa caatttaat cccgaactt atcgcaact 
      acaatacaa aattcagcg ccggaaaca gttcctgtt 
      aaacctaca acgaaacca aaatctgcc tcattagca 
      aaatcgcag cataccctc tacaaccaa tgcattgtt 
      ttattaaat aaagtttca aaacaacaa acggaagcc 
      agatcaaaa actgcaaac cctgcacta acgctcaca 
      aaaaacatc gcgaaactt tcgcgatcg aaagctcaa 
      tcagcaaaa ccgacttac tcaaaaaca aattgcgtc 
      gtaatcaac cgaaagact agagaagat gctgctaaa 
      acgccaaat tcgagcaac gtccgcgaa gccccagat 
      gggcgaccc gaagcggcg tcctgccac cacggggac 
      gacacgtcc cgccacaaa accgataaa acgcagcag 
      caatgggcc acctttgca aggatatgg catgtgtac 
      gatgccgct tggcaggac ccatttcag tccgcgcaa 
      ctgataaaa aagtattta atgggctta tataaacca 
      atttaccac ccaatgaat gattgtggt gaccatgta 
      attgttata aacactaat gagatcgcg atgcctgaa 
      gaagagtgg aagaaacgg gcttatttt caccacacg 
      ggttatcct gggggtgcg tcttggacc ctagcttgg 
      gaattacac agcaaggac ccgaaatta attctcaaa 
      aaagcagtt tataaaagc atggccggc aatttgcag 
      agacggcac acaatggag aggctgcat ctttttgct 
      gatgaaaat gtaccggca gatttgatg aaaaacgtt 
      tctaatcaa ataagacaa cttagacca atgccaaag 
      agactagac cattatact gaagaagaa gttaaatca 
      tttccaaaa cttatcgat tatccgaaa gattacatt 
      ctgaaataa
    • >TC033742 RC
      atgacttta caaggtgtc aagcgaaaa gttgtcacc 
      gtgagcgac ttccggttt acgggacgc ctgctggga 
      aagggaaac tttgcccga gtcgaagaa gccgtccac 
      aacgtcctc aatgtcaag gtggcggtg aagataatc 
      gatatcaga gaaatcaag gaaaagtac gtcgtaaaa 
      aacttgcac agagaagcc cgcataatg tcgacattg 
      aatcacccc tgtatagcg tctcttttc cagaccatg 
      cagttggct gataacgtg tactatctg gttaccgag 
      cttgtttcc ggaggtgat ctttgttct ttcatcatt 
      ggccaacgt tatgggaag ttggaagaa cgcccagca 
      agagtttat gcaagacaa ttcgcttct gctttggct 
      catatgcac agttatgga atcgtccac agagacctc 
      aagatggag aacgtgatg ctgaactcg tcgcagacg 
      cagataaaa atcgtggat ttcggttta agcaacgtc 
      tggaccagc gaaaatcct ctgcgaacc cactgcggg 
      tccccggag tacgccgct ccggagctt ttcattgcc 
      gggagagag tacggaccg gaagtcgac ctctggagt 
      ctggggatc attttctac ggcatggtc gtggggcag 
      ctgcccttt gtcgggggg cgaaaaaat caagtgtcg 
      tcgcaggaa cgcagaaag cgactcgtg gcgcaaatc 
      aacaagggc ctgggggcc acgcaacgc caagctctg 
      gccccccta tcgcccgaa ttccgcacg atgttaaac 
      aaactgcta gcacctgaa ccggtgaaa aggatcacc 
      acgaaggag ctcatcaca catccgtgg atcacggaa 
      aagggccgg aaatgcgtg caaatgcac ccgtttaaa 
      gagctggat aacgacacc cgagcccaa atgattaga 
      aagttgggc gagctgttc caagtggac gcgaccacg 
      gttacgacg gaattgttc aaaaacccg tacgggaga 
      cttggcgga ataaacaac attttgtac tacaaacac 
      ataaacgaa caatttaat cccgaactt atcgcaact 
      acaatacaa aattcagcg ccggaaaca gttcctgtt 
      aaacctaca acgaaacca aaatctgcc tcattagca 
      aaatcgcag cataccctc tacaaccaa tgcattgtt 
      ttattaaat aaagtttca aaacaacaa acggaagcc 
      agatcaaaa actgcaaac cctgcacta acgctcaca 
      aaaaacatc gcgaaactt tcgcgatcg aaagctcaa 
      tcagcaaaa ccgacttac tcaaaaaca aattgcgtc 
      gtaatcaac cgaaagact agagaagat gctgctaaa 
      acgccaaat tcgagcaac gtccgcgaa gccccagat 
      gggcgaccc gaagcggcg tcctgccac cacggggac 
      gacacgtcc cgccacaaa accgataaa acgcagcag 
      tgtgtcgtc agcaatgcc accgatgtg caagtcaag 
      caaccgaga atttcaacg ccgccagat atgcataaa 
      tccagcagg aatgggaga cgcgatacc ctcaaaaag 
      agaactctc cagtgttgc tttgcaaaa gcagcggtc 
      caaggatcc tctagctaa
    mRNA sequence
    • >TC033742 RA
      agctttttt actcagaaa ttatttagc tagttttta 
      caggtctag aacttgcaa aattatcaa aaacaatca 
      agttttaac aactaaaaa tacaatttt ttgtttcgg 
      cttcgtgat tattagacc gcacgctgg atcttacat 
      aacaaatgt aaggttatg aaaactcaa cctcactaa 
      aaagtgtca caaaaaagt aacaataat aacaagcat 
      gtctgtggt aaaaagggt tcagcaatg ggccacctt 
      tgcaaggat atggcatgt gtacgatgc cgcttggca 
      ggacccatt tcagtccgc gcaactgat aaaaaagta 
      tttaatggg cttatataa accaattta ccacccaat 
      gaatgattg tggtgacca tgtaattgt tataaacac 
      taatgagat cgcgatgcc tgaagaaga gtggaagaa 
      acgggctta ttttcacca cacgggtta tcctggggg 
      tgcgtcttg gaccctagc ttgggaatt acacagcaa 
      ggacccgaa attaattct caaaaaagc agtttataa 
      aagcatggc cggcaattt gcagagacg gcacacaat 
      ggagaggct gcatctttt tgctgatga aaatgtacc 
      ggcagattt gatgaaaaa cgtttctaa tcaaataag 
      acaacttag accaatgcc aaagagact agaccatta 
      tactgaaga agaagttaa atcatttcc aaaacttat 
      cgattatcc gaaagatta cattctgaa ataattttt 
      tgttaagaa aatagtttt caaatataa aatagtcac 
      atta
    • >TC033742 RB
      ataccactt tgaaaatgt acaattagt tcaatgagt 
      taattaaaa tttatgttt gttatattt ctaacgtca 
      ttagcaatt cgacttttc tgttcggtg ttgaaatgc 
      ctttgttct cggtttcgg tttcacgat agtgccgta 
      ttaacatga gtttcgcat tagtcgtga ggtatgtgt 
      aaacaagtg taaatgtca tgactttac aaggtgtca 
      agcgaaaag ttgtcaccg tgagcgact tccggttta 
      cgggacgcc tgctgggaa agggaaact ttgcccgag 
      tcgaagaag ccgtccaca acgtcctca atgtcaagg 
      tggcggtga agataatcg atatcagag aaatcaagg 
      aaaagtacg tcgtaaaaa acttgcaca gagaagccc 
      gcataatgt cgacattga atcacccct gtatagcgt 
      ctcttttcc agaccatgc agttggctg ataacgtgt 
      actatctgg ttaccgagc ttgtttccg gaggtgatc 
      tttgttctt tcatcattg gccaacgtt atgggaagt 
      tggaagaac gcccagcaa gagtttatg caagacaat 
      tcgcttctg ctttggctc atatgcaca gttatggaa 
      tcgtccaca gagacctca agatggaga acgtgatgc 
      tgaactcgt cgcagacgc agataaaaa tcgtggatt 
      tcggtttaa gcaacgtct ggaccagcg aaaatcctc 
      tgcgaaccc actgcgggt ccccggagt acgccgctc 
      cggagcttt tcattgccg ggagagagt acggaccgg 
      aagtcgacc tctggagtc tggggatca ttttctacg 
      gcatggtcg tggggcagc tgccctttg tcggggggc 
      gaaaaaatc aagtgtcgt cgcaggaac gcagaaagc 
      gactcgtgg cgcaaatca acaagggcc tgggggcca 
      cgcaacgcc aagctctgg cccccctat cgcccgaat 
      tccgcacga tgttaaaca aactgctag cacctgaac 
      cggtgaaaa ggatcacca cgaaggagc tcatcacac 
      atccgtgga tcacggaaa agggccgga aatgcgtgc 
      aaatgcacc cgtttaaag agctggata acgacaccc 
      gagcccaaa tgattagaa agttgggcg agctgttcc 
      aagtggacg cgaccacgg ttacgacgg aattgttca 
      aaaacccgt acgggagac ttggcggaa taaacaaca 
      ttttgtact acaaacaca taaacgaac aatttaatc 
      ccgaactta tcgcaacta caatacaaa attcagcgc 
      cggaaacag ttcctgtta aacctacaa cgaaaccaa 
      aatctgcct cattagcaa aatcgcagc ataccctct 
      acaaccaat gcattgttt tattaaata aagtttcaa 
      aacaacaaa cggaagcca gatcaaaaa ctgcaaacc 
      ctgcactaa cgctcacaa aaaacatcg cgaaacttt 
      cgcgatcga aagctcaat cagcaaaac cgacttact 
      caaaaacaa attgcgtcg taatcaacc gaaagacta 
      gagaagatg ctgctaaaa cgccaaatt cgagcaacg 
      tccgcgaag ccccagatg ggcgacccg aagcggcgt 
      cctgccacc acggggacg acacgtccc gccacaaaa 
      ccgataaaa cgcagcagc aatgggcca cctttgcaa 
      ggatatggc atgtgtacg atgccgctt ggcaggacc 
      catttcagt ccgcgcaac tgataaaaa agtatttaa 
      tgggcttat ataaaccaa tttaccacc caatgaatg 
      attgtggtg accatgtaa ttgttataa acactaatg 
      agatcgcga tgcctgaag aagagtgga agaaacggg 
      cttattttc accacacgg gttatcctg ggggtgcgt 
      cttggaccc tagcttggg aattacaca gcaaggacc 
      cgaaattaa ttctcaaaa aagcagttt ataaaagca 
      tggccggca atttgcaga gacggcaca caatggaga 
      ggctgcatc tttttgctg atgaaaatg taccggcag 
      atttgatga aaaacgttt ctaatcaaa taagacaac 
      ttagaccaa tgccaaaga gactagacc attatactg 
      aagaagaag ttaaatcat ttccaaaac ttatcgatt 
      atccgaaag attacattc tgaaataat tttttgtta 
      agaaaatag ttttcaaat ataaaatag tcacatta
    • >TC033742 RC
      ataccactt tgaaaatgt acaattagt tcaatgagt 
      taattaaaa tttatgttt gttatattt ctaacgtca 
      ttagcaatt cgacttttc tgttcggtg ttgaaatgc 
      ctttgttct cggtttcgg tttcacgat agtgccgta 
      ttaacatga gtttcgcat tagtcgtga ggtatgtgt 
      aaacaagtg taaatgtca tgactttac aaggtgtca 
      agcgaaaag ttgtcaccg tgagcgact tccggttta 
      cgggacgcc tgctgggaa agggaaact ttgcccgag 
      tcgaagaag ccgtccaca acgtcctca atgtcaagg 
      tggcggtga agataatcg atatcagag aaatcaagg 
      aaaagtacg tcgtaaaaa acttgcaca gagaagccc 
      gcataatgt cgacattga atcacccct gtatagcgt 
      ctcttttcc agaccatgc agttggctg ataacgtgt 
      actatctgg ttaccgagc ttgtttccg gaggtgatc 
      tttgttctt tcatcattg gccaacgtt atgggaagt 
      tggaagaac gcccagcaa gagtttatg caagacaat 
      tcgcttctg ctttggctc atatgcaca gttatggaa 
      tcgtccaca gagacctca agatggaga acgtgatgc 
      tgaactcgt cgcagacgc agataaaaa tcgtggatt 
      tcggtttaa gcaacgtct ggaccagcg aaaatcctc 
      tgcgaaccc actgcgggt ccccggagt acgccgctc 
      cggagcttt tcattgccg ggagagagt acggaccgg 
      aagtcgacc tctggagtc tggggatca ttttctacg 
      gcatggtcg tggggcagc tgccctttg tcggggggc 
      gaaaaaatc aagtgtcgt cgcaggaac gcagaaagc 
      gactcgtgg cgcaaatca acaagggcc tgggggcca 
      cgcaacgcc aagctctgg cccccctat cgcccgaat 
      tccgcacga tgttaaaca aactgctag cacctgaac 
      cggtgaaaa ggatcacca cgaaggagc tcatcacac 
      atccgtgga tcacggaaa agggccgga aatgcgtgc 
      aaatgcacc cgtttaaag agctggata acgacaccc 
      gagcccaaa tgattagaa agttgggcg agctgttcc 
      aagtggacg cgaccacgg ttacgacgg aattgttca 
      aaaacccgt acgggagac ttggcggaa taaacaaca 
      ttttgtact acaaacaca taaacgaac aatttaatc 
      ccgaactta tcgcaacta caatacaaa attcagcgc 
      cggaaacag ttcctgtta aacctacaa cgaaaccaa 
      aatctgcct cattagcaa aatcgcagc ataccctct 
      acaaccaat gcattgttt tattaaata aagtttcaa 
      aacaacaaa cggaagcca gatcaaaaa ctgcaaacc 
      ctgcactaa cgctcacaa aaaacatcg cgaaacttt 
      cgcgatcga aagctcaat cagcaaaac cgacttact 
      caaaaacaa attgcgtcg taatcaacc gaaagacta 
      gagaagatg ctgctaaaa cgccaaatt cgagcaacg 
      tccgcgaag ccccagatg ggcgacccg aagcggcgt 
      cctgccacc acggggacg acacgtccc gccacaaaa 
      ccgataaaa cgcagcagt gtgtcgtca gcaatgcca 
      ccgatgtgc aagtcaagc aaccgagaa tttcaacgc 
      cgccagata tgcataaat ccagcagga atgggagac 
      gcgataccc tcaaaaaga gaactctcc agtgttgct 
      ttgcaaaag cagcggtcc aaggatcct ctagctaac 
      gagccgatc gcgcgatcg atcgctgga tacttaata 
      aaaaatgtt ccagattat atgtttaac gtaaagtgg 
      aggttttgt ttggttgtg tttgtttaa tctttattt 
      cagtcacaa acgatgcgg aaataatac acagtcgcg 
      tctaagtgt aggaattgt aggcgaaag ttggtttcc 
      tacagtgtg aaaaaattg ataaataaa gtaataatt 
      taata
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