Status
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Gene information
General information
TC008936 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 12268506..12278080
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008936
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01440

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands colour not present - Number of animals showing the phenotype: 2 (13 dpi)
    Days post injection



    abdominal odoriferous glands complete not present - Number of animals showing the phenotype: 2 (13 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    Pupal morphology
    head bent - Number of animals showing the phenotype: 6

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008936 RA
      MVPQQNMDS SRSETPISS AVPKFGSQL IDKNSATPY 
      SDATQTKKN NPNHIKRPM NAFMVWSQI ERRKICEVQ 
      PDMHNAEIS KKLGRRWKE LKDDERQPF IEEAERLRQ 
      LHQREYPDY KYRPRKKTT KPAPKSATS SKKSSKKSP 
      KVQKNDNNN NSSNNSNAI VKERLYARR SALPESTSS 
      KLKERLDLS PVMTPLPLP PVSILAKVP SSPSCDIPD 
      SPESATIYD DSLAPLHHE AEDRLAHGS TATLDDLDR 
      IDDFNLLQP MEIDLQELA DIEIMDSGS NGSGSHLDF 
      DCPRDIPIF SSLGGSPWS DGVFNC
    CDS sequence
    • >TC008936 RA
      atggtgccc caacaaaac atggattca tccaggtca 
      gaaacaccg attagtagt gctgtgccc aaattcggc 
      tcacaacta attgacaaa aattcagca acaccctac 
      tctgacgct actcagact aaaaagaac aatcccaac 
      cacatcaaa agacccatg aacgcgttt atggtgtgg 
      tctcagatc gagcgtaga aaaatttgc gaagtgcag 
      ccagacatg cacaatgct gaaatttca aagaagctc 
      ggccgccgc tggaaggag ttgaaagat gacgaacgc 
      cagcctttc atcgaggag gccgaaagg ctccgacag 
      ctccaccag agagagtat ccagattac aagtaccgt 
      cctaggaaa aagacgacg aaaccggcc cccaaatcg 
      gcgacttcc tccaagaaa tcgtccaaa aagtctcca 
      aaagttcaa aagaacgat aacaataac aacagtagt 
      aataacagc aatgcaatc gtgaaagag agattgtac 
      gccagacgc tccgctttg cccgaatcg acgagttcg 
      aagttgaaa gagaggctg gatttatcg ccggtgatg 
      acgcccctt ccactgcca cctgtgagc attttggca 
      aaggtgcca tctagtcct tcttgcgat atcccggat 
      tccccggaa agcgccacg atctacgac gattcgctg 
      gcgccgctg caccacgaa gccgaagac cggctcgcg 
      cacggttcc actgccact ctggacgac ctggacagg 
      atcgacgac ttcaacttg ctgcagccg atggagatt 
      gatctgcag gagctggcg gacatcgag atcatggac 
      agcggctcg aacgggtcc ggctcgcac ctggacttt 
      gactgcccc agagacatc cccatcttc tcgagtctg 
      gggggctcg ccctggagc gacggcgtg ttcaactgt 
      tga
    mRNA sequence
    • >TC008936 RA
      tgtatttga tagtctctt taaatggag attaaacag 
      caccccaga ttgtcagta tcgccacga ctgtaaaga 
      gtagtaggt gttttaatt ttggtaaaa gtgagttcg 
      agaaaaatt gcaaaaagt tcaatcgct cagttttga 
      gacttttcg aactgttga tggtttttg tgcaatctt 
      ctagtcacc cgaaagact acatatttt ttttcgagt 
      tctgaagtt tggggagaa gtttaggaa aaacgcctg 
      cgatttttt acgtaaaac tcgaaattt gtggatttt 
      gttttggag ttcatcagc tggatatcg tcaacaaaa 
      cgcatgctc tccccccac tcctgcggt agaggggat 
      aaacagctg agaatcaga tacacactg acaacatca 
      agttcagct tgtcagtcg cgctgttgt tgtgttact 
      gtcggcaac aaatatgct aagtatagt cgagtgtgc 
      acacacttg agaacttag caagatttt aatgcaaag 
      ttagttgac tgtcgttcc gcccggtag tgtgaagtg 
      aatgtgtac ttagttact aaattggat atggtgccc 
      caacaaaac atggattca tccaggtca gaaacaccg 
      attagtagt gctgtgccc aaattcggc tcacaacta 
      attgacaaa aattcagca acaccctac tctgacgct 
      actcagact aaaaagaac aatcccaac cacatcaaa 
      agacccatg aacgcgttt atggtgtgg tctcagatc 
      gagcgtaga aaaatttgc gaagtgcag ccagacatg 
      cacaatgct gaaatttca aagaagctc ggccgccgc 
      tggaaggag ttgaaagat gacgaacgc cagcctttc 
      atcgaggag gccgaaagg ctccgacag ctccaccag 
      agagagtat ccagattac aagtaccgt cctaggaaa 
      aagacgacg aaaccggcc cccaaatcg gcgacttcc 
      tccaagaaa tcgtccaaa aagtctcca aaagttcaa 
      aagaacgat aacaataac aacagtagt aataacagc 
      aatgcaatc gtgaaagag agattgtac gccagacgc 
      tccgctttg cccgaatcg acgagttcg aagttgaaa 
      gagaggctg gatttatcg ccggtgatg acgcccctt 
      ccactgcca cctgtgagc attttggca aaggtgcca 
      tctagtcct tcttgcgat atcccggat tccccggaa 
      agcgccacg atctacgac gattcgctg gcgccgctg 
      caccacgaa gccgaagac cggctcgcg cacggttcc 
      actgccact ctggacgac ctggacagg atcgacgac 
      ttcaacttg ctgcagccg atggagatt gatctgcag 
      gagctggcg gacatcgag atcatggac agcggctcg 
      aacgggtcc ggctcgcac ctggacttt gactgcccc 
      agagacatc cccatcttc tcgagtctg gggggctcg 
      ccctggagc gacggcgtg ttcaactgt tgaaagagg 
      agactataa gactgtgtt aatccaatt cgtgtgttg 
      gcgcataca atcattgtt atcctcctc tctcggatg 
      gagacggtc cagaggcgg tttcaattg ttctaccag 
      ctcaagttg tcgagccgg ccaggtcga tgatgtttt 
      ataatttta ttatttaac cccgataaa tgatctcat 
      cttgtgttt attttttat atttatcat atagtagcg 
      cgtcagtgt ttcgttcat tcggttcat catcatacg 
      tattcccgg attcaacca gttgttagt gcctagttc 
      agtatgata tgtcggaca taacccggg tcgcccgtc 
      attagtttc gatacttgt aaaatcccg ccggccgcc 
      attttgtac attgttggt ggcagcggg acacaaaat 
      caggctgtg attcatctg tttgatgac caatattta 
      gttgtcaaa agtcagtgt agtttttcc gcctccagc 
      tttgctaat tagttcggg cgcatttag cgacgacgg 
      catacagct gattgtttc gttactgcc aactgcgaa 
      cgcaacgtt tggttttac tcaccttgt atcttatct 
      catcgtttt attcgtcga agaggcgca tctccgcgg 
      ctctgcctt catttttaa taaaaatca tattgtatt 
      gctttctga ttcgttcgt ccagaattg tacttaatg 
      gtcctaaaa tcttgtaca gctgtaagg ttatttaag 
      tgagcactg tgggtttgt tagtggtag gagttgata 
      ttaacagta agatatcca aataggcta tagggttgt 
      ttgtgctgc tgctgctgc tgtcaacga ctcgggcat 
      tcggtttta ctttatctc gtccatcaa actctgttg 
      cacaaaata ttgtttctc tcgttgcct cttgttcca 
      gatgtcaat gctacgagc ttggtttac ctatacata 
      cttatacgc ttaaaactt gtttccgtt caaatctta 
      aagtcaatg cacaaactt tgtaatgag attaaaata 
      gtatgaaaa tttttttca aaaataata gagtagcga 
      gcgacatcg atattcttc tataataac atgatattg 
      aggcacgac caacgctct acgtgtgct ttcaatgta 
      ctagattaa actttacga taatattta aagttagat 
      cggactgtc cgtattata agagttttc gaattttaa 
      aattttatg aggcctggt acagtatga aaatgtaga 
      atataacat attcggtgt atttttgta cccaatgtt 
      tgttcagcg atgcaagtg cagagttgg ctgagacat 
      gtcgctgac tgacttaga ttgtaggtt tttatgttg 
      agctgtact ggttatgag ctgcgagtc gataataac 
      ttctaattt ttaagttca ttgccgttc tttggttcg 
      actgtcttc aggaactct gctctgctc ttgtatttc 
      gcgacttgt atataacgt gtatagatt tgctcatgt 
      aaaaattgt ttttgttga aacgtactg tttatgcaa 
      tcattttaa ataattgca ataactatc gctgcaagt 
      cttgaacgg catcctact gtggactca atactttca 
      acgttcttt gcatatgat gtcacagtg cggcgtcga 
      accagaccc tattgtaaa aaacgttac tttttgtgg 
      cattgttga ccctttttg ttctggtaa acgattgag 
      agattacaa atttagcaa tatttttgc atattatct 
      tgatgtatc ataaaaatc cctgcattt ttgtagacc 
      catgtatta tattcatat tactatatt aaagttatt 
      atatagtgt gtaaatttt gtattgtag ttgctgtta 
      aatatatta attatatgc ccaataaaa gattttttc 
      aatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY