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Gene information
General information
TC008927 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 12036826..12039028
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008927
    iBeetle screen iB_01438

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008927 RA
      MASIDYSYF ISPHSSRRR PALTRELTK RAYGAKDTI 
      SLAEGMPNE VTFPFEKMQ ISMKDGSTL TIQGQQLNA 
      ALQYIPTQG YPPLLKTLK EFTQKVHQP PNWQDREIL 
      ITNGSQDGI SKSIELCVQ EGHPVLVQD PLYTGAEIV 
      LKPFKAELI PVEQDQFGM SAQKLLQVL EDRKAKYTE 
      EGGGCKMPK IMYINPTGS NPTGTTMSL ERRKEIYEI 
      CCKYNILIL EDDAYCFMH YLEENPPSF LSLDTEGRV 
      LRFDSLSKV LSSGLRVGW ITGPKQLIS TIELHIQSS 
      YLHSSTLSQ VIVENLFQA WGFDKFMDH LSFIRKYYK 
      ARRDLTIDS MERHLKGLC EWNVPTGGM FVWIKVRGV 
      SDVYDMLMS RGLKKGITF VPGHAFMAD PTKACSYIR 
      ASYSKANPK QIEKAMKLL AELIREEHL LLRRKLDNC 
      T
    CDS sequence
    • >TC008927 RA
      atggcaagc atcgactac agctatttt atctcccct 
      cactcctcc agaagaaga ccggctctc accagagaa 
      ttaaccaag cgcgcttat ggagccaaa gacacaatc 
      tctttggcc gaaggcatg cccaacgag gtgacattc 
      cctttcgag aaaatgcaa atttcgatg aaagacggc 
      tcaaccctt accatccaa ggacaacag ctgaacgcc 
      gccttacag tacatcccg acccagggc taccctcca 
      ctcctcaaa acgctgaaa gagttcacg caaaaagtc 
      catcagccg ccgaattgg caggaccgc gaaatattg 
      attacaaat gggtcccaa gatggaatc agcaagtcc 
      attgagctc tgcgtccag gaaggtcac ccggtgctg 
      gttcaagac cctctctat accggcgcg gagattgtc 
      ttgaagcca ttcaaggct gaactgatc ccagttgag 
      caagatcag ttcggaatg agcgcccaa aagctgcta 
      caagtgctc gaggaccgg aaagcgaag tatacggaa 
      gagggcgga gggtgtaaa atgcccaag atcatgtac 
      atcaacccc acggggtcg aatccaacg gggacaaca 
      atgtcgtta gaaaggagg aaggagatt tacgaaatc 
      tgttgcaaa tacaacatt ttaattctg gaggacgac 
      gcttattgc ttcatgcac tatttggag gagaacccg 
      cccagcttc ctctctctg gataccgag ggccgtgtg 
      ctccgcttc gattcctta tcaaaagta ttaagttct 
      ggtctgcga gtgggatgg atcactgga ccaaaacaa 
      ctgatctca accatagaa ttacacatt cagagctcc 
      tatctacac tcaagcacc ttatctcag gtcatcgtc 
      gagaacctc ttccaggcc tggggtttc gacaaattc 
      atggaccat ttgagcttc attcgcaag tactacaag 
      gcccgtcga gacctcaca atcgactcg atggagcgc 
      cacttgaag ggcttgtgc gagtggaac gtacccacc 
      ggaggaatg tttgtctgg attaaagtc cggggggtg 
      tccgacgtg tacgacatg ctgatgagc aggggcttg 
      aagaagggt attactttt gtacctggg cacgctttc 
      atggcagat cccaccaaa gcgtgcagc tacattcgc 
      gcttcctac agcaaagcc aatcccaag cagatcgaa 
      aaagcgatg aagcttttg gcggagttg ataagggag 
      gaacatttg ctgttgcgt aggaagcta gataattgt 
      acttaa
    mRNA sequence
    • >TC008927 RA
      cacattcac attcacaac ctcttggcg taaagttgt 
      cccaccgtt gcgtcagct cgttactgt gcagtgcat 
      tttcaagca tttgaaaac cgagaaaat agaaaaatg 
      gcaagcatc gactacagc tattttatc tcccctcac 
      tcctccaga agaagaccg gctctcacc agagaatta 
      accaagcgc gcttatgga gccaaagac acaatctct 
      ttggccgaa ggcatgccc aacgaggtg acattccct 
      ttcgagaaa atgcaaatt tcgatgaaa gacggctca 
      acccttacc atccaagga caacagctg aacgccgcc 
      ttacagtac atcccgacc cagggctac cctccactc 
      ctcaaaacg ctgaaagag ttcacgcaa aaagtccat 
      cagccgccg aattggcag gaccgcgaa atattgatt 
      acaaatggg tcccaagat ggaatcagc aagtccatt 
      gagctctgc gtccaggaa ggtcacccg gtgctggtt 
      caagaccct ctctatacc ggcgcggag attgtcttg 
      aagccattc aaggctgaa ctgatccca gttgagcaa 
      gatcagttc ggaatgagc gcccaaaag ctgctacaa 
      gtgctcgag gaccggaaa gcgaagtat acggaagag 
      ggcggaggg tgtaaaatg cccaagatc atgtacatc 
      aaccccacg gggtcgaat ccaacgggg acaacaatg 
      tcgttagaa aggaggaag gagatttac gaaatctgt 
      tgcaaatac aacatttta attctggag gacgacgct 
      tattgcttc atgcactat ttggaggag aacccgccc 
      agcttcctc tctctggat accgagggc cgtgtgctc 
      cgcttcgat tccttatca aaagtatta agttctggt 
      ctgcgagtg ggatggatc actggacca aaacaactg 
      atctcaacc atagaatta cacattcag agctcctat 
      ctacactca agcacctta tctcaggtc atcgtcgag 
      aacctcttc caggcctgg ggtttcgac aaattcatg 
      gaccatttg agcttcatt cgcaagtac tacaaggcc 
      cgtcgagac ctcacaatc gactcgatg gagcgccac 
      ttgaagggc ttgtgcgag tggaacgta cccaccgga 
      ggaatgttt gtctggatt aaagtccgg ggggtgtcc 
      gacgtgtac gacatgctg atgagcagg ggcttgaag 
      aagggtatt acttttgta cctgggcac gctttcatg 
      gcagatccc accaaagcg tgcagctac attcgcgct 
      tcctacagc aaagccaat cccaagcag atcgaaaaa 
      gcgatgaag cttttggcg gagttgata agggaggaa 
      catttgctg ttgcgtagg aagctagat aattgtact 
      taatttctg agacaaacc ggttaaggt tttctattt 
      gctttgaaa aagcagcct attattatt atactatat 
      gctaggtct ctacagggg ttagaggct tctttttga 
      ttttattgg ttacgctac acatcagca gagggtggt 
      gcggcacaa ctattgatt tttatgact tgcgtctat 
      ataaaaaaa tgtatacaa tatatttag cacattgta 
      gaacttaga agtagattg ttctttatg tagtaatca 
      tgtattgtg gtagttaaa ataaataat ttaacacat 
      tttttgttt tattacttg ttcctcaaa aataaagaa 
      ctggaatga acaatgaac aataaacaa tataagatt 
      gt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY