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Gene information
General information
TC008925 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 11967256..11968544
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008925
    iBeetle screen iB_01437

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008925 RA
      MKKYHQLML LIISIVSLS LFLIYRHEY NRLHYVLEV 
      FNFFGQPCN ISDLQQTEN ILSHHDWGP PPVWQEHES 
      GYIYSAFLT RQNEAKALT LLSDEKLVP RNCYFWFED 
      KKKPVIGKF KFSKVMSDE NVRLNLYFY ICEMSNSEH 
      VPYAVSFSF KTRKDSELK KIMLTNSFN HQVNINTTI 
      CVSPSIYSK RRLVEFISY HKLIGIDSF IFYNRDVPH 
      RLVKIITNL STRLGLQAA FLPWNFPKG DSLVTRSVV 
      ENDCLLRTF GQTSYAVTL ELNEYIVPL HSYSFNTFL 
      KNTDSESGR ISLPVQKFC IENTDTAKP ISMVNTDVS 
      DDYNYNVIV RYIYKNNQR KEDISTYAV DKGFASIHK 
      YVRCSFEPS KTRKDKSIL KFSTDLTRS TLFQLLLHN 
      QI
    CDS sequence
    • >TC008925 RA
      atgaaaaag taccaccag ttgatgtta ttaattatt 
      tccattgtt agtctgagt ttgttcctg atttaccgg 
      cacgagtac aaccgcctg cattatgta ttagaagtg 
      tttaacttt ttcgggcaa ccgtgcaac atctccgac 
      ctgcagcag accgaaaac atactgagc caccacgac 
      tggggcccg ccgcccgtc tggcaggag cacgaaagt 
      ggctacatt tactccgca tttctcacc cggcagaac 
      gaagccaag gcattgact ttgctcagc gatgagaaa 
      ctggtgcct cggaactgc tacttctgg tttgaggac 
      aagaagaag ccagttatt gggaaattc aagttttcc 
      aaagtgatg agtgacgag aacgtccga ctgaatttg 
      tatttctac atttgtgag atgagcaac agtgaacat 
      gtgccatat gcggtctcg ttcagtttc aagacgcgc 
      aaagatagc gagttgaag aaaatcatg ttgacgaat 
      tcgttcaat catcaggtg aatattaac acgacgatt 
      tgcgtctcg ccgtcgatt tacagcaag cggcggctt 
      gtggagttc ataagctat cataaattg atcggaatc 
      gactcgttt attttttat aacagggat gttccgcat 
      cggttggtt aaaattatc acgaatctc tcgacaagg 
      ctcggtttg caagccgcg ttcttgccg tggaatttc 
      ccgaaaggg gacagtctg gtcacgcgg tcagtcgtc 
      gaaaatgat tgtctcttg cggacgttc gggcagact 
      tcgtacgcc gtcactttg gagttaaac gagtacatt 
      gtacctttg cacagctac agttttaat acttttttg 
      aaaaatacc gacagcgag tcgggccgg atcagtctg 
      cccgtgcaa aaattctgt attgagaac accgatacg 
      gcaaaaccg atttcaatg gtaaatacg gacgtttca 
      gatgactac aattataac gtaatagtt cgctatatt 
      tacaagaac aatcaaagg aaggaggat atttccact 
      tatgccgtc gataagggg tttgcctcc atacacaag 
      tacgtgaga tgttcgttt gaaccttcc aaaacgcgc 
      aaagacaag tccattttg aagttttcc actgatctc 
      actcggtcc acgcttttc caactcctt ttgcacaat 
      caaatttaa
    mRNA sequence
    • >TC008925 RA
      tatgtttat gttaacctc aaaatcgca caatgtcaa 
      acgaactga gctaaagaa tgaaaaagt accaccagt 
      tgatgttat taattattt ccattgtta gtctgagtt 
      tgttcctga tttaccggc acgagtaca accgcctgc 
      attatgtat tagaagtgt ttaactttt tcgggcaac 
      cgtgcaaca tctccgacc tgcagcaga ccgaaaaca 
      tactgagcc accacgact ggggcccgc cgcccgtct 
      ggcaggagc acgaaagtg gctacattt actccgcat 
      ttctcaccc ggcagaacg aagccaagg cattgactt 
      tgctcagcg atgagaaac tggtgcctc ggaactgct 
      acttctggt ttgaggaca agaagaagc cagttattg 
      ggaaattca agttttcca aagtgatga gtgacgaga 
      acgtccgac tgaatttgt atttctaca tttgtgaga 
      tgagcaaca gtgaacatg tgccatatg cggtctcgt 
      tcagtttca agacgcgca aagatagcg agttgaaga 
      aaatcatgt tgacgaatt cgttcaatc atcaggtga 
      atattaaca cgacgattt gcgtctcgc cgtcgattt 
      acagcaagc ggcggcttg tggagttca taagctatc 
      ataaattga tcggaatcg actcgttta ttttttata 
      acagggatg ttccgcatc ggttggtta aaattatca 
      cgaatctct cgacaaggc tcggtttgc aagccgcgt 
      tcttgccgt ggaatttcc cgaaagggg acagtctgg 
      tcacgcggt cagtcgtcg aaaatgatt gtctcttgc 
      ggacgttcg ggcagactt cgtacgccg tcactttgg 
      agttaaacg agtacattg tacctttgc acagctaca 
      gttttaata cttttttga aaaataccg acagcgagt 
      cgggccgga tcagtctgc ccgtgcaaa aattctgta 
      ttgagaaca ccgatacgg caaaaccga tttcaatgg 
      taaatacgg acgtttcag atgactaca attataacg 
      taatagttc gctatattt acaagaaca atcaaagga 
      aggaggata tttccactt atgccgtcg ataaggggt 
      ttgcctcca tacacaagt acgtgagat gttcgtttg 
      aaccttcca aaacgcgca aagacaagt ccattttga 
      agttttcca ctgatctca ctcggtcca cgcttttcc 
      aactccttt tgcacaatc aaatttaaa aagagagca 
      agtggaagc aataaacat cttatgagg aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY