Status
Please wait ...
Gene information
General information
TC008899 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid49: 4797835..4801731
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008899
    iBeetle screen iB_01434
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008899 RA
      MAQWVGYMV SIKCADDTA YQGEISAAT TSQIALTKA 
      FCNGIPCPS NEVTIRANE IEELKLIDK HLSSPPQRS 
      TITIAKPIA KRAGRTQST SDVANAASK HTNLNGSHS 
      KSKPIDIEP KTTNYSFNE SSSFKNGTP VRREKSKGK 
      WSKGWKDEE CFGSPLDHK INKDFDFEK NLALFDKQA 
      IWDEINNSQ KPDVIKNAD SKKPAKYRH DENVIASTP 
      ASYRQIIVP KQDFCEYVT DDGLIIPSI SRSLRKKLW 
      EVADRVGLT WDKRVELFG RAAAEISVQ LLGGGHRLN 
      PHNSHQFPT VVVLCGPHK QGAMGINAA RQLASHGVH 
      TIVYVVSSE VAVIKKELA LYVLTKNPV IYSVTNLPN 
      FADLIIVAL CEDTESPKS YPDLCDWAS RNRASILAL 
      DPPSVGIPG IAPKFSLLP VLPLPHSSK NGKLYLCNL 
      GFPLEIFNE VGIKYKSPF GPKFVIPLH PKDD
    CDS sequence
    • >TC008899 RA
      atggcacag tgggtagga tacatggtc tcaattaag 
      tgcgccgat gataccgct tatcaaggt gaaattagc 
      gcagctacg acttctcag attgctcta actaaggct 
      ttctgcaac ggcattccc tgtccttcg aacgaagtg 
      accattagg gcaaacgaa atcgaagag ttgaaatta 
      attgacaag cacttaagc tcacctcca cagagaagc 
      acaataacc atcgccaag ccaatcgcg aagcgcgct 
      ggacgaacg cagagcacc tcagatgtc gccaatgca 
      gcatccaaa catacaaat ttaaacgga tctcacagc 
      aaatcaaaa cccattgat atcgaaccc aaaaccacg 
      aactatagt tttaacgaa tccagttca ttcaaaaac 
      ggaacccca gtcaggcga gaaaagtca aaaggcaag 
      tggtccaaa ggctggaag gacgaggag tgtttcggc 
      tcgcctttg gaccacaaa atcaacaaa gacttcgat 
      tttgagaaa aatttggcg ctttttgat aaacaagcc 
      atctgggac gagattaac aactcccag aaaccggac 
      gttattaag aatgccgac agcaagaag ccggcgaaa 
      tacaggcat gatgaaaat gttattgca agcactcca 
      gcatcgtat aggcagata atagtgcct aagcaagac 
      ttttgcgag tatgtgacg gatgatggg ctgattata 
      ccgagcatt tcgaggtct ttgcgaaag aaactgtgg 
      gaagtggct gacagagtg gggctcacg tgggataag 
      agggtggag ctgtttggg agggcagct gccgaaatt 
      tcggtgcag ttgttgggg ggcgggcat aggttgaat 
      cctcacaac tcgcaccag tttcccact gttgtagtg 
      ctgtgtggg ccgcacaag cagggggcg atgggcatc 
      aacgccgcg cgccaactg gccagtcac ggcgtgcac 
      accatcgtc tacgtggtc tcctcggag gtggcggtg 
      ataaaaaaa gagctggcg ctctacgtc ctcaccaaa 
      aacccggta atctattcg gtgacgaat ctcccgaat 
      ttcgccgat ttgataata gttgcgtta tgcgaggac 
      acggaaagt ccaaagagt tatcccgat ttgtgcgat 
      tgggctagt agaaataga gcgtcaatt ttagcttta 
      gatccccct tctgttggt atacctggc atagccccc 
      aagttttcc ttactccca gttttacct ctccctcat 
      tcttccaaa aatggaaag ttgtacttg tgcaacctg 
      ggattcccc ctcgaaata ttcaatgag gtggggatc 
      aagtataag tcgcccttt ggccccaaa tttgtcata 
      cctttgcat ccaaaggat gactag
    mRNA sequence
    • >TC008899 RA
      agagaaagc tgtatcgaa aagtgtgtc gcaagagat 
      ggttgttgg gtgctgtta atttgttgt gggttcatt 
      gttgtaaat atttcttct tgttttgtg ctaattatt 
      ggttctaca tcacgtatt taataattt gttgggaag 
      ttgaggtcg actgataaa tctgctgcg cagtgttac 
      ctaacctca ttttataaa ttatacaaa ttatctgct 
      ctcggttgt gctattgat atttgtaaa gaaaatggc 
      acagtgggt aggatacat ggtctcaat taagtgcgc 
      cgatgatac cgcttatca aggtgaaat tagcgcagc 
      tacgacttc tcagattgc tctaactaa ggctttctg 
      caacggcat tccctgtcc ttcgaacga agtgaccat 
      tagggcaaa cgaaatcga agagttgaa attaattga 
      caagcactt aagctcacc tccacagag aagcacaat 
      aaccatcgc caagccaat cgcgaagcg cgctggacg 
      aacgcagag cacctcaga tgtcgccaa tgcagcatc 
      caaacatac aaatttaaa cggatctca cagcaaatc 
      aaaacccat tgatatcga acccaaaac cacgaacta 
      tagttttaa cgaatccag ttcattcaa aaacggaac 
      cccagtcag gcgagaaaa gtcaaaagg caagtggtc 
      caaaggctg gaaggacga ggagtgttt cggctcgcc 
      tttggacca caaaatcaa caaagactt cgattttga 
      gaaaaattt ggcgctttt tgataaaca agccatctg 
      ggacgagat taacaactc ccagaaacc ggacgttat 
      taagaatgc cgacagcaa gaagccggc gaaatacag 
      gcatgatga aaatgttat tgcaagcac tccagcatc 
      gtataggca gataatagt gcctaagca agacttttg 
      cgagtatgt gacggatga tgggctgat tataccgag 
      catttcgag gtctttgcg aaagaaact gtgggaagt 
      ggctgacag agtggggct cacgtggga taagagggt 
      ggagctgtt tgggagggc agctgccga aatttcggt 
      gcagttgtt ggggggcgg gcataggtt gaatcctca 
      caactcgca ccagtttcc cactgttgt agtgctgtg 
      tgggccgca caagcaggg ggcgatggg catcaacgc 
      cgcgcgcca actggccag tcacggcgt gcacaccat 
      cgtctacgt ggtctcctc ggaggtggc ggtgataaa 
      aaaagagct ggcgctcta cgtcctcac caaaaaccc 
      ggtaatcta ttcggtgac gaatctccc gaatttcgc 
      cgatttgat aatagttgc gttatgcga ggacacgga 
      aagtccaaa gagttatcc cgatttgtg cgattgggc 
      tagtagaaa tagagcgtc aattttagc tttagatcc 
      cccttctgt tggtatacc tggcatagc ccccaagtt 
      ttccttact cccagtttt acctctccc tcattcttc 
      caaaaatgg aaagttgta cttgtgcaa cctgggatt 
      ccccctcga aatattcaa tgaggtggg gatcaagta 
      taagtcgcc ctttggccc caaatttgt cataccttt 
      gcatccaaa ggatgacta ggaattggt acaacagca 
      aagctattt tattctttt gacacattt cttacgtga 
      atgcagatt tggaagtaa ttagggttt ttacttaat 
      gacacgtcc agtgtgatt ttttgaaaa ttttcaaga 
      cattccaag ttgtctcaa aataccagt tgcatattt 
      ttttattac atcatgtat gttgccatc tttatcata 
      cactttaat tattcggtt ttatggaga attttattt 
      aaattgtga tgaaaattc aatcgataa gaaacgtga 
      taagatatt gttttgaat tcaactttt tttcagtca 
      acaaattca ttaacagcc attattttt gattaattt 
      tgtttgtaa cgttacaaa gcgaaataa aagtgcatt 
      ttccaa