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Gene information
General information
TC008895 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid49: 4755874..4757629
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008895
    iBeetle screen iB_01433
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008895 RA
      MAEQTLTQF TDKLLSEKI FSCAVCEKL LTLPIVLIE 
      DVGNVCPNC CEDRDWKGL HNVKLEMIL KELQIPCKF 
      QSTGCKKRV HFSALNEHE SNCKFHEKP CLLSHTGCE 
      WTGVQSDFP SHFNECHSE HVIANPQSF FVIEININE 
      ETNVTKLLK GKQECIIIV KKDREDLLH TICDVKNTE 
      CNYDCTIKH FGQSSNCVE TKCKVSSFS SMHTTRINL 
      AALKQMTEA ADVVTLQIK MSTQNEAAE FDEKVLQYF 
      ECPVCKMLM KPPIYQCKF GHSFCSNCR PRLENCPNC 
      RALFGTTRN YALEGLTAG ISYACMYHH LGCEEMLPA 
      HDSGKHEAI CPFKPYPCP LDDCSFKGT HSNIGKHLD 
      ENHKDKVIA ADFYKTTVE FRLEQMIDF YNFHQKYYM 
      VFDENIFRL SFKRNSDYS FWAVEVLRK KEEDAVFIY 
      EIGIIDMRK PDRRLIRCD VCFTDTNSE ELFKKCILF 
      PNNILASYA CNGVFTYYC QIRKRQ
    CDS sequence
    • >TC008895 RA
      atggcagaa caaacccta actcagttc acagacaag 
      ctactttcg gaaaaaata ttcagttgt gctgtttgc 
      gagaaactg cttacgctc ccgatagtg ctgatagaa 
      gatgtgggg aacgtttgc cccaattgt tgtgaggac 
      agggactgg aaagggctg cacaatgtc aagctggag 
      atgatttta aaggagctt caaatcccg tgcaagttt 
      cagtcaacg ggttgcaag aaaagggtt catttcagt 
      gctttaaac gagcacgaa agtaactgt aagtttcat 
      gagaaacct tgtttgttg tcacacacc ggttgtgaa 
      tggacagga gtccagagc gattttcct agtcacttt 
      aacgaatgc cactctgag catgtgata gcaaaccct 
      cagagcttc tttgtcatt gagatcaac ataaacgag 
      gaaacgaac gtaacgaag ctgctcaaa ggcaagcaa 
      gagtgcata ataattgtg aaaaaagat cgcgaagat 
      ttgttgcac acaatctgt gatgtaaaa aacactgaa 
      tgtaattat gattgtact atcaagcat ttcggacaa 
      agtagcaac tgcgttgaa acaaagtgc aaagtttca 
      tcattcagt agcatgcac accacaagg attaactta 
      gcggctttg aaacagatg accgaggcc gctgatgtg 
      gtcacactg caaataaaa atgtccacg cagaacgaa 
      gccgcggaa tttgatgaa aaagtcctc caatatttc 
      gaatgcccc gtttgcaaa atgctgatg aagccgcca 
      atttatcag tgcaagttc ggccacagt ttttgtagc 
      aactgtagg ccaagattg gaaaactgt ccgaattgt 
      cgcgccctg ttcggaacc acgagaaat tacgccttg 
      gaaggcttg actgcagga atcagttat gcgtgcatg 
      taccaccat ctggggtgc gaggagatg ctgccagct 
      catgattcg ggcaaacac gaggcgatt tgtccgttt 
      aaaccgtac ccctgtcct ttggacgac tgttcattt 
      aagggaacc cactcgaat attggaaaa cacctggat 
      gagaatcac aaggataaa gtcattgcg gccgatttc 
      tacaaaact accgtggag tttcgtttg gagcaaatg 
      atcgacttt tacaacttc caccagaag tattacatg 
      gtgtttgat gaaaatata tttagactg agtttcaag 
      cgcaattca gattatagt ttctgggcg gttgaagtg 
      ttgcgtaaa aaggaagaa gacgctgtt tttatttac 
      gaaattgga atcattgat atgagaaaa cctgataga 
      agactgata agatgcgat gtgtgcttt actgatact 
      aattctgaa gaactgttt aaaaagtgt attttgttt 
      ccgaataat atcctggct tcttatgct tgtaacggg 
      gtgtttaca tactattgt caaatacgc aaacggcaa 
      taa
    mRNA sequence
    • >TC008895 RA
      cgttcaaaa aatttttgt ttcgaaatt ggggttatc 
      tgacctgtc acttcttaa cttatattg tgcaaaaac 
      agcatgata gccagttca aatgtggtc atttagtgt 
      ggagtcgca gcagggttt tggatatgg cagaacaaa 
      ccctaactc agttcacag acaagctac tttcggaaa 
      aaatattca gttgtgctg tttgcgaga aactgctta 
      cgctcccga tagtgctga tagaagatg tggggaacg 
      tttgcccca attgttgtg aggacaggg actggaaag 
      ggctgcaca atgtcaagc tggagatga ttttaaagg 
      agcttcaaa tcccgtgca agtttcagt caacgggtt 
      gcaagaaaa gggttcatt tcagtgctt taaacgagc 
      acgaaagta actgtaagt ttcatgaga aaccttgtt 
      tgttgtcac acaccggtt gtgaatgga caggagtcc 
      agagcgatt ttcctagtc actttaacg aatgccact 
      ctgagcatg tgatagcaa accctcaga gcttctttg 
      tcattgaga tcaacataa acgaggaaa cgaacgtaa 
      cgaagctgc tcaaaggca agcaagagt gcataataa 
      ttgtgaaaa aagatcgcg aagatttgt tgcacacaa 
      tctgtgatg taaaaaaca ctgaatgta attatgatt 
      gtactatca agcatttcg gacaaagta gcaactgcg 
      ttgaaacaa agtgcaaag tttcatcat tcagtagca 
      tgcacacca caaggatta acttagcgg ctttgaaac 
      agatgaccg aggccgctg atgtggtca cactgcaaa 
      taaaaatgt ccacgcaga acgaagccg cggaatttg 
      atgaaaaag tcctccaat atttcgaat gccccgttt 
      gcaaaatgc tgatgaagc cgccaattt atcagtgca 
      agttcggcc acagttttt gtagcaact gtaggccaa 
      gattggaaa actgtccga attgtcgcg ccctgttcg 
      gaaccacga gaaattacg ccttggaag gcttgactg 
      caggaatca gttatgcgt gcatgtacc accatctgg 
      ggtgcgagg agatgctgc cagctcatg attcgggca 
      aacacgagg cgatttgtc cgtttaaac cgtacccct 
      gtcctttgg acgactgtt catttaagg gaacccact 
      cgaatattg gaaaacacc tggatgaga atcacaagg 
      ataaagtca ttgcggccg atttctaca aaactaccg 
      tggagtttc gtttggagc aaatgatcg acttttaca 
      acttccacc agaagtatt acatggtgt ttgatgaaa 
      atatattta gactgagtt tcaagcgca attcagatt 
      atagtttct gggcggttg aagtgttgc gtaaaaagg 
      aagaagacg ctgttttta tttacgaaa ttggaatca 
      ttgatatga gaaaacctg atagaagac tgataagat 
      gcgatgtgt gctttactg atactaatt ctgaagaac 
      tgtttaaaa agtgtattt tgtttccga ataatatcc 
      tggcttctt atgcttgta acggggtgt ttacatact 
      attgtcaaa tacgcaaac ggcaataaa ttgcaaagt 
      acaaaaccg agttttctt taacaaaaa ataaatgtg 
      gagataaaa c