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Gene information
General information
TC008880 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 11287480..11289099
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008880
    iBeetle screen iB_01432

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008880 RA
      MAQSAPLIL RLLASSVTA ANRAGKIIR DIMARGDLG 
      IVDKGINDL QTEADRSAQ RCIVASLSQ QYPDLTIIG 
      EEGANNSEV PSDWIVTDY DKVVISKQC PPEYSTVTS 
      EDIVVWVDP LDGTSEYTQ GLLEHVTVL VGLSVKGKA 
      IAGVIHQPY YNYKSGNSI GRTIWGLVG MGVGGFDPQ 
      GAPPGKLIL TTTRSHSDG TVQKALEAL NPDEIIKVG 
      GAGHKVLLL IEGKAHAYV FASKGCKKW DTCAPEAVL 
      VATGGKLTD MHGNAYNYG KDVQHPNTG GVFATVSGL 
      DHEGLLAKI PQEVKDKLK
    CDS sequence
    • >TC008880 RA
      atggctcaa agcgctcct ctaattttg cgtttactg 
      gcatcttcc gtaaccgcg gccaatagg gcgggaaaa 
      ataatacgg gatataatg gccagggga gacttgggg 
      attgtcgat aagggcatt aatgatctt caaactgaa 
      gcagacaga tctgcacaa cgctgtatc gtggcttct 
      ttatctcag caatatcca gatttgacc ataattggg 
      gaagaagga gcaaataat tctgaagtg ccaagcgac 
      tggattgta acagactat gacaaagtg gtaatctca 
      aaacagtgc cccccggaa tattccacc gtaacaagt 
      gaggatatt gtggtctgg gtggacccc cttgatggc 
      acatcagag tacacccag gggctcttg gaacatgtc 
      acagtatta gtaggtttg tcggtgaaa ggcaaagcc 
      atagctggt gtcatacac cagccctac tacaattac 
      aaaagcggc aactcaata gggcgcaca atttggggg 
      ctcgtgggc atgggggtc gggggcttt gacccccaa 
      ggggcgccc cctggcaaa cttattctc actacgacc 
      aggtcacac tctgatggt acagtccag aaagccctc 
      gaggctcta aatccagac gaaattatc aaagttgga 
      ggtgcaggc cataaagtg ttgttactg atcgagggc 
      aaagcccac gcgtatgtc tttgcaagc aaaggctgc 
      aaaaaatgg gacacttgc gcccccgaa gcagtgcta 
      gtggctact gggggtaaa ctaacagat atgcacggg 
      aacgcttat aactatggc aaagatgtt cagcaccct 
      aacacaggg ggggtgttt gcgaccgtg agtggtttg 
      gaccatgaa ggtttgtta gcgaaaatt ccacaagaa 
      gttaaagac aaactcaag tga
    mRNA sequence
    • >TC008880 RA
      cactcgaat gtcacaata aaacagaaa ttatttttt 
      taaatcgac ttatttgga tcaattagc tcgagaaca 
      gtttacaga aagaaatac aatggctca aagcgctcc 
      tctaatttt gcgtttact ggcatcttc cgtaaccgc 
      ggccaatag ggcgggaaa aataatacg ggatataat 
      ggccagggg agacttggg gattgtcga taagggcat 
      taatgatct tcaaactga agcagacag atctgcaca 
      acgctgtat cgtggcttc tttatctca gcaatatcc 
      agatttgac cataattgg ggaagaagg agcaaataa 
      ttctgaagt gccaagcga ctggattgt aacagacta 
      tgacaaagt ggtaatctc aaaacagtg ccccccgga 
      atattccac cgtaacaag tgaggatat tgtggtctg 
      ggtggaccc ccttgatgg cacatcaga gtacaccca 
      ggggctctt ggaacatgt cacagtatt agtaggttt 
      gtcggtgaa aggcaaagc catagctgg tgtcataca 
      ccagcccta ctacaatta caaaagcgg caactcaat 
      agggcgcac aatttgggg gctcgtggg catgggggt 
      cgggggctt tgaccccca aggggcgcc ccctggcaa 
      acttattct cactacgac caggtcaca ctctgatgg 
      tacagtcca gaaagccct cgaggctct aaatccaga 
      cgaaattat caaagttgg aggtgcagg ccataaagt 
      gttgttact gatcgaggg caaagccca cgcgtatgt 
      ctttgcaag caaaggctg caaaaaatg ggacacttg 
      cgcccccga agcagtgct agtggctac tgggggtaa 
      actaacaga tatgcacgg gaacgctta taactatgg 
      caaagatgt tcagcaccc taacacagg gggggtgtt 
      tgcgaccgt gagtggttt ggaccatga aggtttgtt 
      agcgaaaat tccacaaga agttaaaga caaactcaa 
      gtgatgtct ttcacgctt tttaattct tgtctccta 
      atcatttga tgtatttat gttacaaaa tgggcaata 
      aagagaatc agcaaaaag ttgttttcc cgaaataat 
      aaaaattaa acatgaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY