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Gene information
General information
TC008872 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid49: 4581533..4584330
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008872
    iBeetle screen iB_01430
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008872 RA
      MASYIARNI VKSPAALGL TQQQFGNLR AFSVGTQLN 
      SKKTVPDRT GKNIVLVDG VRTPFLMSG TDYSKLMPH 
      DLAKQALAG ILKKTGVPK EMIDYVIYG TVIQEVKTS 
      NIAREAALA AGYSDKTPA HTVTMACIS SNVAMTTAI 
      GLIASGVYE LIVAGGVEF MSDIPIRHN RKMRSLMLQ 
      ANKAKTLQQ KLGLLASIR PDYFVPELP AVAEFSSGE 
      TMGHSADRL AAAFGATRR EQDEFALRS HTLAQHAYE 
      SGHFTDLIP VKIPGIDKV IEKDNGIRV STPEQLAKL 
      KPAFIKPHG TVTAANASF LTDGASAAI VTTEAKAKA 
      LGLKPKAYL RDFIYVSQD PIDQLLLGP AYGTPIVLE 
      KAGLTMKDI AVWEFHEAF AGQILANFK AMDSDWFAQ 
      TYMKRSSKI GAPDINKFN NWGGSLSIG HPFAATGIR 
      LAMHTANRL VKEDGQFGV VAACAAGGQ GVAMILERH 
      PEATAN
    CDS sequence
    • >TC008872 RA
      atggcttcg tacattgca agaaacata gttaaaagc 
      cctgcagcc ctagggcta acccagcaa caatttgga 
      aatttgagg gccttttct gtcggaacg caattaaac 
      agcaagaaa actgttcca gacagaact ggaaagaat 
      atcgttttg gtggatgga gtcagaacc ccattttta 
      atgtctgga acagactat tccaaactg atgccacat 
      gacttagcc aaacaagcc ttagctggc attttaaaa 
      aagactggt gttccaaag gaaatgatt gattatgtc 
      atctatgga acggttatt caggaagtc aagacttca 
      aatattgcg agggaggct gcccttgca gctggttac 
      agtgacaaa acccccgct cataccgtc acaatggct 
      tgcatttca tcaaatgtg gccatgact acagccatt 
      gggcttatt gcaagcggt gtttacgag ctcatcgtg 
      gcaggaggt gttgaattc atgtctgat attcccatt 
      aggcacaac agaaaaatg cgcagcttg atgctccaa 
      gccaacaaa gccaagact ttgcagcaa aaattgggg 
      cttctggcg agcatcaga cctgattat ttcgtccca 
      gagttgcca gctgttgcc gagttttct tctggcgaa 
      accatgggt cactctgct gaccgtctc gcagctgcc 
      tttggggcc actcgtaga gaacaagac gaattcgct 
      cttaggtca cacactttg gcgcaacat gcatatgaa 
      agtggccac ttcaccgat cttatccca gtcaagatc 
      cctggaatt gataaagtt attgaaaaa gataacgga 
      atccgtgtt tccacccca gaacaactg gccaaactt 
      aagcctgca tttattaaa ccacacggt accgtcaca 
      gcagccaat gcctcattt ttgactgac ggtgcttcc 
      gctgctatt gttacaacg gaagcgaaa gctaaggct 
      ttgggcctg aaacccaag gcctatttg cgggacttt 
      atctacgtt tcccaagac cctatcgac caacttttg 
      ctaggacca gcctacggc actccaatt gtattagaa 
      aaagccggg cttacaatg aaagacatt gctgtttgg 
      gagttccat gaagctttc gccggccaa attttggca 
      aatttcaaa gccatggac agtgattgg tttgcacaa 
      acttatatg aagaggagc agcaagatt ggggctcct 
      gacatcaac aaattcaac aactgggga ggatcgctt 
      tctattgga catcccttc gctgccact ggaatcagg 
      ctggctatg cacaccgct aataggctt gtgaaagag 
      gatggacag tttggagtg gttgctgct tgcgcagct 
      ggtggacag ggtgtggcc atgattttg gaaagacat 
      cctgaggcc accgcaaat taa
    mRNA sequence
    • >TC008872 RA
      acactgcgg gtcaaagtc gacgtgtgg cgaatctac 
      cttttagta aatattccg aaattgtgt tcctttgcg 
      ttaattttt atttgatat tttaaccct tcaaaatgg 
      cttcgtaca ttgcaagaa acatagtta aaagccctg 
      cagccctag ggctaaccc agcaacaat ttggaaatt 
      tgagggcct tttctgtcg gaacgcaat taaacagca 
      agaaaactg ttccagaca gaactggaa agaatatcg 
      ttttggtgg atggagtca gaaccccat ttttaatgt 
      ctggaacag actattcca aactgatgc cacatgact 
      tagccaaac aagccttag ctggcattt taaaaaaga 
      ctggtgttc caaaggaaa tgattgatt atgtcatct 
      atggaacgg ttattcagg aagtcaaga cttcaaata 
      ttgcgaggg aggctgccc ttgcagctg gttacagtg 
      acaaaaccc ccgctcata ccgtcacaa tggcttgca 
      tttcatcaa atgtggcca tgactacag ccattgggc 
      ttattgcaa gcggtgttt acgagctca tcgtggcag 
      gaggtgttg aattcatgt ctgatattc ccattaggc 
      acaacagaa aaatgcgca gcttgatgc tccaagcca 
      acaaagcca agactttgc agcaaaaat tggggcttc 
      tggcgagca tcagacctg attatttcg tcccagagt 
      tgccagctg ttgccgagt tttcttctg gcgaaacca 
      tgggtcact ctgctgacc gtctcgcag ctgcctttg 
      gggccactc gtagagaac aagacgaat tcgctctta 
      ggtcacaca ctttggcgc aacatgcat atgaaagtg 
      gccacttca ccgatctta tcccagtca agatccctg 
      gaattgata aagttattg aaaaagata acggaatcc 
      gtgtttcca ccccagaac aactggcca aacttaagc 
      ctgcattta ttaaaccac acggtaccg tcacagcag 
      ccaatgcct catttttga ctgacggtg cttccgctg 
      ctattgtta caacggaag cgaaagcta aggctttgg 
      gcctgaaac ccaaggcct atttgcggg actttatct 
      acgtttccc aagacccta tcgaccaac ttttgctag 
      gaccagcct acggcactc caattgtat tagaaaaag 
      ccgggctta caatgaaag acattgctg tttgggagt 
      tccatgaag ctttcgccg gccaaattt tggcaaatt 
      tcaaagcca tggacagtg attggtttg cacaaactt 
      atatgaaga ggagcagca agattgggg ctcctgaca 
      tcaacaaat tcaacaact ggggaggat cgctttcta 
      ttggacatc ccttcgctg ccactggaa tcaggctgg 
      ctatgcaca ccgctaata ggcttgtga aagaggatg 
      gacagtttg gagtggttg ctgcttgcg cagctggtg 
      gacagggtg tggccatga ttttggaaa gacatcctg 
      aggccaccg caaattaat actttttat atttatttt 
      tatttttcc cgcaagtgt ccttaagtt ttcatcagt 
      acttttata gtccctcca actcctcta gagaaacaa 
      ctgtacata gcttttcta tacattatt taattaata 
      aaaaaggtt cttttataa tgtaata