Status
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Gene information
General information
TC008869 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 11128724..11131646
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008869
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01429

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult nutritional state - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 7 (16 dpi)
    Days post injection



    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008869 RA
      MGLLTDPSA GQGKLTKAL LKYYTKLCV LLYIGGIGW 
      FCSLAYTPM NAGTYFSEN ALLPGLVKS EFREDAIAK 
      TYHSELLDE MKKYEDSIP YPWLLAKMK QIGLDTYTH 
      NFTLNYPLG KPQKFVGKN VYGILRAAR ASSTEALVL 
      SVPYRPPLS VHATTAPSI AIMLAFAKF ANREKYWAK 
      DIIFLITEH EQLGMQAWL EAYHGVACG NDGTLDHGD 
      IKGRAGAIQ AAINLELHE TAISQVDIK IEGLNGQLP 
      NLDLFNLAA KMLLKEGVP HTFKNRPNK HLKDPVNDW 
      FYSFQTLLA MISTQATGI PNGNHGLYH RFGIEALTL 
      ESTPLKGGN KAGFPIVGR IIEGIFRSL NNLLERFHQ 
      SFFFYLLPS SERFISIGL YMPAICLIA GALFIKACA 
      NWCQLQENQ AETEDEKTK TPETKKQVK SSVKPRDSK 
      NYWKEMETR FQTEIQTHN PPDPDSVNF ISILCVFLM 
      SHVLGVLIM NSPQLLTTL GGQYNYSTD ASLFYGFIA 
      ISVVLLTMP LFIPFKACE KSMSVLNIL SLLELGTTL 
      ICVSMNNFS LALFCAIIY VPLALIIGI TRCRLCSAI 
      KKIVWLLAH PLVVLTATV LVYTFFMFP GEKFEDNFH 
      RGVAATQQA IVFSIVDSM IYGNWLFSV VTSVFISNW 
      LCFWIVAFA KVPQDIPKK KID
    CDS sequence
    • >TC008869 RA
      atgggtttg ctaacagat cccagtgcc ggtcaggga 
      aaactaacc aaagccctt cttaaatat tacacaaaa 
      ctatgtgtc ttgctatac ataggcgga atcggttgg 
      ttctgttcc cttgcgtac acccccatg aacgcaggg 
      acgtatttt tccgaaaac gcccttttg ccaggactc 
      gtcaaatca gagtttcgg gaagacgca atcgcaaaa 
      acttaccac agcgagctt ttggacgaa atgaagaaa 
      tacgaagac tccattccg tatccctgg cttttagcc 
      aaaatgaag caaattgga ttagatacc tacactcac 
      aactttaca ctgaattat ccgctggga aaaccccaa 
      aaatttgtt ggtaaaaac gtttatggt attttacga 
      gcggcacgg gcttccagc actgaagct ttagtctta 
      agtgtgcca tatagaccc cctttgagc gtccacgcc 
      accaccgca ccttccatt gccataatg ttggcattt 
      gccaagttt gccaataga gagaaatat tgggccaag 
      gatattata tttttaatt actgaacac gagcagtta 
      ggaatgcaa gcttggctg gaagcctac catggagta 
      gcctgtggc aatgacggt actttagac catggtgac 
      attaaggga cgtgcaggt gccatacaa gccgctatt 
      aatttagaa ttgcacgaa acggcaatt agccaagtg 
      gatatcaaa attgaaggt ttaaatggt caactgccc 
      aacttggat ttgtttaac ttagccgct aaaatgctg 
      ctcaaagag ggagtaccg catactttt aaaaacagg 
      cccaacaaa cacttgaaa gatcccgta aacgactgg 
      ttttactca ttccagact ttattagcc atgatttca 
      actcaggct accgggata ccaaatgga aatcatggc 
      ctttatcac cgttttggc atagaagcc ctgactctt 
      gaaagcacc cccttaaaa gggggcaat aaagccgga 
      ttcccaatt gtgggccgc attattgaa gggatattc 
      cgtagcttg aataacctt ttagagcgt ttccatcaa 
      agctttttc ttctacttg ttaccttca tcagagaga 
      tttatttca attgggcta tacatgcct gctatctgt 
      ttgattgcg ggggctctc ttcatcaaa gcatgcgcc 
      aattggtgc caacttcag gagaaccaa gcggagacg 
      gaagacgag aagacaaaa acccccgag acgaaaaaa 
      caagtcaag agcagcgtg aaaccgcgc gattcgaaa 
      aattattgg aaagaaatg gaaacgcga ttccagacc 
      gaaattcaa actcacaac cctccagat cccgattcc 
      gttaacttt atttcaatc ctttgcgta tttctaatg 
      tctcatgtt ctgggggta ttgattatg aacagccct 
      cagctatta acgacgcta ggtggccaa tataattac 
      tcaaccgat gcgtctttg ttttacggg tttatagcc 
      atatctgtg gtgttgcta acaatgccg ctttttatc 
      cccttcaaa gcttgcgag aagagtatg tcggtgtta 
      aatatttta tcgcttttg gaattaggc acaacttta 
      atttgcgtt tccatgaat aatttttct ctggcatta 
      ttttgcgcc attatttat gtaccgttg gctctaatt 
      atcgggatt acgcggtgt aggctatgc tctgccatc 
      aaaaaaatt gtgtggttg ctggcacat cctctagtc 
      gtgcttacc gctaccgtg ttagtatac acatttttc 
      atgttcccc ggtgaaaaa ttcgaagat aattttcat 
      aggggcgta gcggcgact caacaggca atcgttttc 
      agtatagtg gattctatg atttatgga aattggctc 
      tttagcgtc gttactagt gtttttatt tccaactgg 
      ttgtgtttc tggatcgtt gcgtttgcg aaagtgccg 
      caggacatt ccaaagaag aaaatcgat taa
    mRNA sequence
    • >TC008869 RA
      ttacccctt tatcttggc cacttcgtt tgtggcttc 
      aaaggcggg cagttttaa accgctggt gttttttta 
      gacagttgc gttggtagc aaccaaacg tcaagttat 
      ctactctaa ggttatgtt aatgtaaac aaaaattat 
      ctaaacggc tcctgtaat gtaattagt ggctaattt 
      tgactgtga agtttcaat tccagctct tttttggat 
      aaagtttaa gtcacgatg ggtttgcta acagatccc 
      agtgccggt cagggaaaa ctaaccaaa gcccttctt 
      aaatattac acaaaacta tgtgtcttg ctatacata 
      ggcggaatc ggttggttc tgttccctt gcgtacacc 
      cccatgaac gcagggacg tatttttcc gaaaacgcc 
      cttttgcca ggactcgtc aaatcagag tttcgggaa 
      gacgcaatc gcaaaaact taccacagc gagcttttg 
      gacgaaatg aagaaatac gaagactcc attccgtat 
      ccctggctt ttagccaaa atgaagcaa attggatta 
      gatacctac actcacaac tttacactg aattatccg 
      ctgggaaaa ccccaaaaa tttgttggt aaaaacgtt 
      tatggtatt ttacgagcg gcacgggct tccagcact 
      gaagcttta gtcttaagt gtgccatat agaccccct 
      ttgagcgtc cacgccacc accgcacct tccattgcc 
      ataatgttg gcatttgcc aagtttgcc aatagagag 
      aaatattgg gccaaggat attatattt ttaattact 
      gaacacgag cagttagga atgcaagct tggctggaa 
      gcctaccat ggagtagcc tgtggcaat gacggtact 
      ttagaccat ggtgacatt aagggacgt gcaggtgcc 
      atacaagcc gctattaat ttagaattg cacgaaacg 
      gcaattagc caagtggat atcaaaatt gaaggttta 
      aatggtcaa ctgcccaac ttggatttg tttaactta 
      gccgctaaa atgctgctc aaagaggga gtaccgcat 
      acttttaaa aacaggccc aacaaacac ttgaaagat 
      cccgtaaac gactggttt tactcattc cagacttta 
      ttagccatg atttcaact caggctacc gggatacca 
      aatggaaat catggcctt tatcaccgt tttggcata 
      gaagccctg actcttgaa agcaccccc ttaaaaggg 
      ggcaataaa gccggattc ccaattgtg ggccgcatt 
      attgaaggg atattccgt agcttgaat aacctttta 
      gagcgtttc catcaaagc tttttcttc tacttgtta 
      ccttcatca gagagattt atttcaatt gggctatac 
      atgcctgct atctgtttg attgcgggg gctctcttc 
      atcaaagca tgcgccaat tggtgccaa cttcaggag 
      aaccaagcg gagacggaa gacgagaag acaaaaacc 
      cccgagacg aaaaaacaa gtcaagagc agcgtgaaa 
      ccgcgcgat tcgaaaaat tattggaaa gaaatggaa 
      acgcgattc cagaccgaa attcaaact cacaaccct 
      ccagatccc gattccgtt aactttatt tcaatcctt 
      tgcgtattt ctaatgtct catgttctg ggggtattg 
      attatgaac agccctcag ctattaacg acgctaggt 
      ggccaatat aattactca accgatgcg tctttgttt 
      tacgggttt atagccata tctgtggtg ttgctaaca 
      atgccgctt tttatcccc ttcaaagct tgcgagaag 
      agtatgtcg gtgttaaat attttatcg cttttggaa 
      ttaggcaca actttaatt tgcgtttcc atgaataat 
      ttttctctg gcattattt tgcgccatt atttatgta 
      ccgttggct ctaattatc gggattacg cggtgtagg 
      ctatgctct gccatcaaa aaaattgtg tggttgctg 
      gcacatcct ctagtcgtg cttaccgct accgtgtta 
      gtatacaca tttttcatg ttccccggt gaaaaattc 
      gaagataat tttcatagg ggcgtagcg gcgactcaa 
      caggcaatc gttttcagt atagtggat tctatgatt 
      tatggaaat tggctcttt agcgtcgtt actagtgtt 
      tttatttcc aactggttg tgtttctgg atcgttgcg 
      tttgcgaaa gtgccgcag gacattcca aagaagaaa 
      atcgattaa atgtacttt tttttaata aagtgttgg 
      attttta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY