General information | |||
TC008869 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 11128724..11131646 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC008869 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01429 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
ovary without detectable defects - Number of animals showing the phenotype: 1
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 7 (11 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 7 (16 dpi)
pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (19 dpi)
- >TC008869 RA
MGLLTDPSA GQGKLTKAL LKYYTKLCV LLYIGGIGW FCSLAYTPM NAGTYFSEN ALLPGLVKS EFREDAIAK TYHSELLDE MKKYEDSIP YPWLLAKMK QIGLDTYTH NFTLNYPLG KPQKFVGKN VYGILRAAR ASSTEALVL SVPYRPPLS VHATTAPSI AIMLAFAKF ANREKYWAK DIIFLITEH EQLGMQAWL EAYHGVACG NDGTLDHGD IKGRAGAIQ AAINLELHE TAISQVDIK IEGLNGQLP NLDLFNLAA KMLLKEGVP HTFKNRPNK HLKDPVNDW FYSFQTLLA MISTQATGI PNGNHGLYH RFGIEALTL ESTPLKGGN KAGFPIVGR IIEGIFRSL NNLLERFHQ SFFFYLLPS SERFISIGL YMPAICLIA GALFIKACA NWCQLQENQ AETEDEKTK TPETKKQVK SSVKPRDSK NYWKEMETR FQTEIQTHN PPDPDSVNF ISILCVFLM SHVLGVLIM NSPQLLTTL GGQYNYSTD ASLFYGFIA ISVVLLTMP LFIPFKACE KSMSVLNIL SLLELGTTL ICVSMNNFS LALFCAIIY VPLALIIGI TRCRLCSAI KKIVWLLAH PLVVLTATV LVYTFFMFP GEKFEDNFH RGVAATQQA IVFSIVDSM IYGNWLFSV VTSVFISNW LCFWIVAFA KVPQDIPKK KID
- >TC008869 RA
atgggtttg ctaacagat cccagtgcc ggtcaggga aaactaacc aaagccctt cttaaatat tacacaaaa ctatgtgtc ttgctatac ataggcgga atcggttgg ttctgttcc cttgcgtac acccccatg aacgcaggg acgtatttt tccgaaaac gcccttttg ccaggactc gtcaaatca gagtttcgg gaagacgca atcgcaaaa acttaccac agcgagctt ttggacgaa atgaagaaa tacgaagac tccattccg tatccctgg cttttagcc aaaatgaag caaattgga ttagatacc tacactcac aactttaca ctgaattat ccgctggga aaaccccaa aaatttgtt ggtaaaaac gtttatggt attttacga gcggcacgg gcttccagc actgaagct ttagtctta agtgtgcca tatagaccc cctttgagc gtccacgcc accaccgca ccttccatt gccataatg ttggcattt gccaagttt gccaataga gagaaatat tgggccaag gatattata tttttaatt actgaacac gagcagtta ggaatgcaa gcttggctg gaagcctac catggagta gcctgtggc aatgacggt actttagac catggtgac attaaggga cgtgcaggt gccatacaa gccgctatt aatttagaa ttgcacgaa acggcaatt agccaagtg gatatcaaa attgaaggt ttaaatggt caactgccc aacttggat ttgtttaac ttagccgct aaaatgctg ctcaaagag ggagtaccg catactttt aaaaacagg cccaacaaa cacttgaaa gatcccgta aacgactgg ttttactca ttccagact ttattagcc atgatttca actcaggct accgggata ccaaatgga aatcatggc ctttatcac cgttttggc atagaagcc ctgactctt gaaagcacc cccttaaaa gggggcaat aaagccgga ttcccaatt gtgggccgc attattgaa gggatattc cgtagcttg aataacctt ttagagcgt ttccatcaa agctttttc ttctacttg ttaccttca tcagagaga tttatttca attgggcta tacatgcct gctatctgt ttgattgcg ggggctctc ttcatcaaa gcatgcgcc aattggtgc caacttcag gagaaccaa gcggagacg gaagacgag aagacaaaa acccccgag acgaaaaaa caagtcaag agcagcgtg aaaccgcgc gattcgaaa aattattgg aaagaaatg gaaacgcga ttccagacc gaaattcaa actcacaac cctccagat cccgattcc gttaacttt atttcaatc ctttgcgta tttctaatg tctcatgtt ctgggggta ttgattatg aacagccct cagctatta acgacgcta ggtggccaa tataattac tcaaccgat gcgtctttg ttttacggg tttatagcc atatctgtg gtgttgcta acaatgccg ctttttatc cccttcaaa gcttgcgag aagagtatg tcggtgtta aatatttta tcgcttttg gaattaggc acaacttta atttgcgtt tccatgaat aatttttct ctggcatta ttttgcgcc attatttat gtaccgttg gctctaatt atcgggatt acgcggtgt aggctatgc tctgccatc aaaaaaatt gtgtggttg ctggcacat cctctagtc gtgcttacc gctaccgtg ttagtatac acatttttc atgttcccc ggtgaaaaa ttcgaagat aattttcat aggggcgta gcggcgact caacaggca atcgttttc agtatagtg gattctatg atttatgga aattggctc tttagcgtc gttactagt gtttttatt tccaactgg ttgtgtttc tggatcgtt gcgtttgcg aaagtgccg caggacatt ccaaagaag aaaatcgat taa
- >TC008869 RA
ttacccctt tatcttggc cacttcgtt tgtggcttc aaaggcggg cagttttaa accgctggt gttttttta gacagttgc gttggtagc aaccaaacg tcaagttat ctactctaa ggttatgtt aatgtaaac aaaaattat ctaaacggc tcctgtaat gtaattagt ggctaattt tgactgtga agtttcaat tccagctct tttttggat aaagtttaa gtcacgatg ggtttgcta acagatccc agtgccggt cagggaaaa ctaaccaaa gcccttctt aaatattac acaaaacta tgtgtcttg ctatacata ggcggaatc ggttggttc tgttccctt gcgtacacc cccatgaac gcagggacg tatttttcc gaaaacgcc cttttgcca ggactcgtc aaatcagag tttcgggaa gacgcaatc gcaaaaact taccacagc gagcttttg gacgaaatg aagaaatac gaagactcc attccgtat ccctggctt ttagccaaa atgaagcaa attggatta gatacctac actcacaac tttacactg aattatccg ctgggaaaa ccccaaaaa tttgttggt aaaaacgtt tatggtatt ttacgagcg gcacgggct tccagcact gaagcttta gtcttaagt gtgccatat agaccccct ttgagcgtc cacgccacc accgcacct tccattgcc ataatgttg gcatttgcc aagtttgcc aatagagag aaatattgg gccaaggat attatattt ttaattact gaacacgag cagttagga atgcaagct tggctggaa gcctaccat ggagtagcc tgtggcaat gacggtact ttagaccat ggtgacatt aagggacgt gcaggtgcc atacaagcc gctattaat ttagaattg cacgaaacg gcaattagc caagtggat atcaaaatt gaaggttta aatggtcaa ctgcccaac ttggatttg tttaactta gccgctaaa atgctgctc aaagaggga gtaccgcat acttttaaa aacaggccc aacaaacac ttgaaagat cccgtaaac gactggttt tactcattc cagacttta ttagccatg atttcaact caggctacc gggatacca aatggaaat catggcctt tatcaccgt tttggcata gaagccctg actcttgaa agcaccccc ttaaaaggg ggcaataaa gccggattc ccaattgtg ggccgcatt attgaaggg atattccgt agcttgaat aacctttta gagcgtttc catcaaagc tttttcttc tacttgtta ccttcatca gagagattt atttcaatt gggctatac atgcctgct atctgtttg attgcgggg gctctcttc atcaaagca tgcgccaat tggtgccaa cttcaggag aaccaagcg gagacggaa gacgagaag acaaaaacc cccgagacg aaaaaacaa gtcaagagc agcgtgaaa ccgcgcgat tcgaaaaat tattggaaa gaaatggaa acgcgattc cagaccgaa attcaaact cacaaccct ccagatccc gattccgtt aactttatt tcaatcctt tgcgtattt ctaatgtct catgttctg ggggtattg attatgaac agccctcag ctattaacg acgctaggt ggccaatat aattactca accgatgcg tctttgttt tacgggttt atagccata tctgtggtg ttgctaaca atgccgctt tttatcccc ttcaaagct tgcgagaag agtatgtcg gtgttaaat attttatcg cttttggaa ttaggcaca actttaatt tgcgtttcc atgaataat ttttctctg gcattattt tgcgccatt atttatgta ccgttggct ctaattatc gggattacg cggtgtagg ctatgctct gccatcaaa aaaattgtg tggttgctg gcacatcct ctagtcgtg cttaccgct accgtgtta gtatacaca tttttcatg ttccccggt gaaaaattc gaagataat tttcatagg ggcgtagcg gcgactcaa caggcaatc gttttcagt atagtggat tctatgatt tatggaaat tggctcttt agcgtcgtt actagtgtt tttatttcc aactggttg tgtttctgg atcgttgcg tttgcgaaa gtgccgcag gacattcca aagaagaaa atcgattaa atgtacttt tttttaata aagtgttgg attttta
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
