Status
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Gene information
General information
TC008869 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 11128724..11131646
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC008869
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01429

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    adult nutritional state - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 7 (16 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008869 RA
      MGLLTDPSA GQGKLTKAL LKYYTKLCV LLYIGGIGW 
FCSLAYTPM NAGTYFSEN ALLPGLVKS EFREDAIAK 
TYHSELLDE MKKYEDSIP YPWLLAKMK QIGLDTYTH 
NFTLNYPLG KPQKFVGKN VYGILRAAR ASSTEALVL 
SVPYRPPLS VHATTAPSI AIMLAFAKF ANREKYWAK 
DIIFLITEH EQLGMQAWL EAYHGVACG NDGTLDHGD 
IKGRAGAIQ AAINLELHE TAISQVDIK IEGLNGQLP 
NLDLFNLAA KMLLKEGVP HTFKNRPNK HLKDPVNDW 
FYSFQTLLA MISTQATGI PNGNHGLYH RFGIEALTL 
ESTPLKGGN KAGFPIVGR IIEGIFRSL NNLLERFHQ 
SFFFYLLPS SERFISIGL YMPAICLIA GALFIKACA 
NWCQLQENQ AETEDEKTK TPETKKQVK SSVKPRDSK 
NYWKEMETR FQTEIQTHN PPDPDSVNF ISILCVFLM 
SHVLGVLIM NSPQLLTTL GGQYNYSTD ASLFYGFIA 
ISVVLLTMP LFIPFKACE KSMSVLNIL SLLELGTTL 
ICVSMNNFS LALFCAIIY VPLALIIGI TRCRLCSAI 
KKIVWLLAH PLVVLTATV LVYTFFMFP GEKFEDNFH 
RGVAATQQA IVFSIVDSM IYGNWLFSV VTSVFISNW 
LCFWIVAFA KVPQDIPKK KID
    CDS sequence
    • >TC008869 RA
      atgggtttg ctaacagat cccagtgcc ggtcaggga 
aaactaacc aaagccctt cttaaatat tacacaaaa 
ctatgtgtc ttgctatac ataggcgga atcggttgg 
ttctgttcc cttgcgtac acccccatg aacgcaggg 
acgtatttt tccgaaaac gcccttttg ccaggactc 
gtcaaatca gagtttcgg gaagacgca atcgcaaaa 
acttaccac agcgagctt ttggacgaa atgaagaaa 
tacgaagac tccattccg tatccctgg cttttagcc 
aaaatgaag caaattgga ttagatacc tacactcac 
aactttaca ctgaattat ccgctggga aaaccccaa 
aaatttgtt ggtaaaaac gtttatggt attttacga 
gcggcacgg gcttccagc actgaagct ttagtctta 
agtgtgcca tatagaccc cctttgagc gtccacgcc 
accaccgca ccttccatt gccataatg ttggcattt 
gccaagttt gccaataga gagaaatat tgggccaag 
gatattata tttttaatt actgaacac gagcagtta 
ggaatgcaa gcttggctg gaagcctac catggagta 
gcctgtggc aatgacggt actttagac catggtgac 
attaaggga cgtgcaggt gccatacaa gccgctatt 
aatttagaa ttgcacgaa acggcaatt agccaagtg 
gatatcaaa attgaaggt ttaaatggt caactgccc 
aacttggat ttgtttaac ttagccgct aaaatgctg 
ctcaaagag ggagtaccg catactttt aaaaacagg 
cccaacaaa cacttgaaa gatcccgta aacgactgg 
ttttactca ttccagact ttattagcc atgatttca 
actcaggct accgggata ccaaatgga aatcatggc 
ctttatcac cgttttggc atagaagcc ctgactctt 
gaaagcacc cccttaaaa gggggcaat aaagccgga 
ttcccaatt gtgggccgc attattgaa gggatattc 
cgtagcttg aataacctt ttagagcgt ttccatcaa 
agctttttc ttctacttg ttaccttca tcagagaga 
tttatttca attgggcta tacatgcct gctatctgt 
ttgattgcg ggggctctc ttcatcaaa gcatgcgcc 
aattggtgc caacttcag gagaaccaa gcggagacg 
gaagacgag aagacaaaa acccccgag acgaaaaaa 
caagtcaag agcagcgtg aaaccgcgc gattcgaaa 
aattattgg aaagaaatg gaaacgcga ttccagacc 
gaaattcaa actcacaac cctccagat cccgattcc 
gttaacttt atttcaatc ctttgcgta tttctaatg 
tctcatgtt ctgggggta ttgattatg aacagccct 
cagctatta acgacgcta ggtggccaa tataattac 
tcaaccgat gcgtctttg ttttacggg tttatagcc 
atatctgtg gtgttgcta acaatgccg ctttttatc 
cccttcaaa gcttgcgag aagagtatg tcggtgtta 
aatatttta tcgcttttg gaattaggc acaacttta 
atttgcgtt tccatgaat aatttttct ctggcatta 
ttttgcgcc attatttat gtaccgttg gctctaatt 
atcgggatt acgcggtgt aggctatgc tctgccatc 
aaaaaaatt gtgtggttg ctggcacat cctctagtc 
gtgcttacc gctaccgtg ttagtatac acatttttc 
atgttcccc ggtgaaaaa ttcgaagat aattttcat 
aggggcgta gcggcgact caacaggca atcgttttc 
agtatagtg gattctatg atttatgga aattggctc 
tttagcgtc gttactagt gtttttatt tccaactgg 
ttgtgtttc tggatcgtt gcgtttgcg aaagtgccg 
caggacatt ccaaagaag aaaatcgat taa
    mRNA sequence
    • >TC008869 RA
      ttacccctt tatcttggc cacttcgtt tgtggcttc 
aaaggcggg cagttttaa accgctggt gttttttta 
gacagttgc gttggtagc aaccaaacg tcaagttat 
ctactctaa ggttatgtt aatgtaaac aaaaattat 
ctaaacggc tcctgtaat gtaattagt ggctaattt 
tgactgtga agtttcaat tccagctct tttttggat 
aaagtttaa gtcacgatg ggtttgcta acagatccc 
agtgccggt cagggaaaa ctaaccaaa gcccttctt 
aaatattac acaaaacta tgtgtcttg ctatacata 
ggcggaatc ggttggttc tgttccctt gcgtacacc 
cccatgaac gcagggacg tatttttcc gaaaacgcc 
cttttgcca ggactcgtc aaatcagag tttcgggaa 
gacgcaatc gcaaaaact taccacagc gagcttttg 
gacgaaatg aagaaatac gaagactcc attccgtat 
ccctggctt ttagccaaa atgaagcaa attggatta 
gatacctac actcacaac tttacactg aattatccg 
ctgggaaaa ccccaaaaa tttgttggt aaaaacgtt 
tatggtatt ttacgagcg gcacgggct tccagcact 
gaagcttta gtcttaagt gtgccatat agaccccct 
ttgagcgtc cacgccacc accgcacct tccattgcc 
ataatgttg gcatttgcc aagtttgcc aatagagag 
aaatattgg gccaaggat attatattt ttaattact 
gaacacgag cagttagga atgcaagct tggctggaa 
gcctaccat ggagtagcc tgtggcaat gacggtact 
ttagaccat ggtgacatt aagggacgt gcaggtgcc 
atacaagcc gctattaat ttagaattg cacgaaacg 
gcaattagc caagtggat atcaaaatt gaaggttta 
aatggtcaa ctgcccaac ttggatttg tttaactta 
gccgctaaa atgctgctc aaagaggga gtaccgcat 
acttttaaa aacaggccc aacaaacac ttgaaagat 
cccgtaaac gactggttt tactcattc cagacttta 
ttagccatg atttcaact caggctacc gggatacca 
aatggaaat catggcctt tatcaccgt tttggcata 
gaagccctg actcttgaa agcaccccc ttaaaaggg 
ggcaataaa gccggattc ccaattgtg ggccgcatt 
attgaaggg atattccgt agcttgaat aacctttta 
gagcgtttc catcaaagc tttttcttc tacttgtta 
ccttcatca gagagattt atttcaatt gggctatac 
atgcctgct atctgtttg attgcgggg gctctcttc 
atcaaagca tgcgccaat tggtgccaa cttcaggag 
aaccaagcg gagacggaa gacgagaag acaaaaacc 
cccgagacg aaaaaacaa gtcaagagc agcgtgaaa 
ccgcgcgat tcgaaaaat tattggaaa gaaatggaa 
acgcgattc cagaccgaa attcaaact cacaaccct 
ccagatccc gattccgtt aactttatt tcaatcctt 
tgcgtattt ctaatgtct catgttctg ggggtattg 
attatgaac agccctcag ctattaacg acgctaggt 
ggccaatat aattactca accgatgcg tctttgttt 
tacgggttt atagccata tctgtggtg ttgctaaca 
atgccgctt tttatcccc ttcaaagct tgcgagaag 
agtatgtcg gtgttaaat attttatcg cttttggaa 
ttaggcaca actttaatt tgcgtttcc atgaataat 
ttttctctg gcattattt tgcgccatt atttatgta 
ccgttggct ctaattatc gggattacg cggtgtagg 
ctatgctct gccatcaaa aaaattgtg tggttgctg 
gcacatcct ctagtcgtg cttaccgct accgtgtta 
gtatacaca tttttcatg ttccccggt gaaaaattc 
gaagataat tttcatagg ggcgtagcg gcgactcaa 
caggcaatc gttttcagt atagtggat tctatgatt 
tatggaaat tggctcttt agcgtcgtt actagtgtt 
tttatttcc aactggttg tgtttctgg atcgttgcg 
tttgcgaaa gtgccgcag gacattcca aagaagaaa 
atcgattaa atgtacttt tttttaata aagtgttgg 
attttta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY