| General information | |||
| TC008849 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 10747703..10757634 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC008849 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01427 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
ovary without detectable defects - Number of animals showing the phenotype: 2
- >TC008849 RA
MWCLISQAN SVVFEVRVD PKAIGQECL EKVCEFVNV QYESDYFGL KYENHKGEE SWLNLRNPI DRQVTFHGH TSYLRLSLR VKFWVPPHI LQQESTRHQ FYLNARLHL LEKRLTAPD WASISRLIA FIAQAECGD FNELDPPRN IYNLATTIA PLDQSEKPG DLLQRIVHE HKKCKGMKS SSAEYWFLK EVSEFENFG EETFTVKNH QNNNTVYGI GPHGITIYK KDGEKELIS FTNITSASS HKRTFKLEY LTIDNEGAI LNIKLDSSH VASSLYRAI TEKHAFYSC ETVRSAVTA QFIRDLKGT IVSIFNEDS TLGKKYVFD IRRTCREVY DNARRALYL ENTIQEITR ANLDYNGNA GECKNSQEK LSRFLDAMT CKICMDGQI DAIFLPCGH GVACMACAS RCDRCPLCR SDIAQAKKV FLPIDYLLV GQTTN
- >TC008849 RA
atgtggtgt ctcatcagc caggccaat tcggtcgtt ttcgaagtc cgggtcgac cccaaagcc atcggccag gaatgtctc gagaaggtg tgcgaattc gtgaatgtg caatacgag tctgattac ttcggattg aagtacgaa aaccacaag ggagaggaa tcctggtta aatttacga aaccccatc gatcggcaa gtgactttt cacggccat acgtcctat ctgcgctta tcactgcgg gtgaagttc tgggttcct cctcacatc ttgcagcaa gaaagcacc cgtcaccag ttttacctc aacgcgaga cttcatctg ttggaaaaa cgcttaaca gccccggac tgggccagc atttcaaga ctgatcgct tttatagct caagctgaa tgtggcgac ttcaacgaa cttgatccg cctcggaat atttacaat ttagctaca acgatcgct cctttggat cagtcggag aaacccgga gatttgcta cagaggatc gtccacgag cacaaaaaa tgcaaaggg atgaagagc tcttccgcc gagtattgg tttttgaag gaagtgtcg gagtttgag aatttcgga gaggagacg ttcacggtg aaaaatcac cagaataac aacacggtg tacgggatc ggccctcat ggcatcacg atttacaaa aaggacggg gagaaggag ttgatttcg ttcactaac ataacgagt gcttcctcc cataagcgc acttttaaa cttgaatat ttgaccatc gataacgaa ggggctatt ctaaatatt aaactagat tctagccat gtggctagt agcttatat cgggccatt acggaaaaa cacgccttc tacagctgc gagacggtt cgcagtgcc gtaacggca caatttatt cgagatctg aaagggacc attgtttca attttcaac gaggactcc acattgggc aaaaagtac gtgttcgat attcggagg acctgcaga gaagtctac gacaatgca agaagagcc ttgtatttg gaaaacact attcaggaa atcactcgc gccaatctc gactacaac ggcaacgcg ggagagtgc aagaattcc caagaaaaa ttgtcgaga tttctggac gcaatgacc tgtaaaatc tgcatggac ggccagatc gacgcgatt ttcctgccg tgcgggcac ggggtggcc tgcatggcg tgcgcctcg cgctgcgac aggtgcccc ctttgtcgt tccgacatc gcacaagcg aagaaggtg tttttgccc attgactac ctcctggtg ggtcagact acgaattga
- >TC008849 RA
tgagtcagg tgcgttcgc tgttcgcgt gcgctgtca ccccgtatg tctgagctg aaccaaata aatgtgaac aaggaactt cgatttaat attgtccaa gctgttgca tagtatgga cgttgtcgt aaaatgtgc aaagagttt atacaacaa acgatacgc cgtcagtat atcagaaca ttgcgattt tgtcaaatg atcagtggc ttctgctag cgatttttc gtctagcca attttgagg ttcatttta cgaattttt ggaaaatgt ggtgtctca tcagccagg ccaattcgg tcgttttcg aagtccggg tcgacccca aagccatcg gccaggaat gtctcgaga aggtgtgcg aattcgtga atgtgcaat acgagtctg attacttcg gattgaagt acgaaaacc acaagggag aggaatcct ggttaaatt tacgaaacc ccatcgatc ggcaagtga cttttcacg gccatacgt cctatctgc gcttatcac tgcgggtga agttctggg ttcctcctc acatcttgc agcaagaaa gcacccgtc accagtttt acctcaacg cgagacttc atctgttgg aaaaacgct taacagccc cggactggg ccagcattt caagactga tcgctttta tagctcaag ctgaatgtg gcgacttca acgaacttg atccgcctc ggaatattt acaatttag ctacaacga tcgctcctt tggatcagt cggagaaac ccggagatt tgctacaga ggatcgtcc acgagcaca aaaaatgca aagggatga agagctctt ccgccgagt attggtttt tgaaggaag tgtcggagt ttgagaatt tcggagagg agacgttca cggtgaaaa atcaccaga ataacaaca cggtgtacg ggatcggcc ctcatggca tcacgattt acaaaaagg acggggaga aggagttga tttcgttca ctaacataa cgagtgctt cctcccata agcgcactt ttaaacttg aatatttga ccatcgata acgaagggg ctattctaa atattaaac tagattcta gccatgtgg ctagtagct tatatcggg ccattacgg aaaaacacg ccttctaca gctgcgaga cggttcgca gtgccgtaa cggcacaat ttattcgag atctgaaag ggaccattg tttcaattt tcaacgagg actccacat tgggcaaaa agtacgtgt tcgatattc ggaggacct gcagagaag tctacgaca atgcaagaa gagccttgt atttggaaa acactattc aggaaatca ctcgcgcca atctcgact acaacggca acgcgggag agtgcaaga attcccaag aaaaattgt cgagatttc tggacgcaa tgacctgta aaatctgca tggacggcc agatcgacg cgattttcc tgccgtgcg ggcacgggg tggcctgca tggcgtgcg cctcgcgct gcgacaggt gcccccttt gtcgttccg acatcgcac aagcgaaga aggtgtttt tgcccattg actacctcc tggtgggtc agactacga attgaaaga ctgcatttt tactttact aagatctcg aatagttta gttgtggaa gtggacaga gtacacaag tgacaatcg tataatttt tgatctttt cttgtgatg ttttgttaa ccaaaatct catattgag gtttgtttt gtacattgt taagtatct atctacata ctatttttt actatgtaa agtatgaag gacctaaag tgttgtatt ttattaact attgacaat agtaagaaa atgcaaaag ctatcgaaa tgtaaatga catgaatta ttaatagtc atattatca atgcaagat gtaaattag gtttagttt cgatatttg ttaatataa taaaaatgt tataatgct agtattata atgagaaat gtgtgataa gaaattttt gttttgtga tattgcatt catttggaa aattaagtt tacgtttat actatcaaa ttttattaa tcgatgtta aagtaaagt gtagacgtg tatatgtca catatttac aagtatttt gttattagt attataacg agtatatct gttaaattt tttgctatt cacgtttac gtcaacatg gaattacaa taattattt atgttgttt tatttcaaa aaaaacctt ccttattta ctaataaag gtaagtgga attg
