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Gene information
General information
TC008849 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10747703..10757634
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008849
    iBeetle screen iB_01427

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 2 - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008849 RA
      MWCLISQAN SVVFEVRVD PKAIGQECL EKVCEFVNV 
      QYESDYFGL KYENHKGEE SWLNLRNPI DRQVTFHGH 
      TSYLRLSLR VKFWVPPHI LQQESTRHQ FYLNARLHL 
      LEKRLTAPD WASISRLIA FIAQAECGD FNELDPPRN 
      IYNLATTIA PLDQSEKPG DLLQRIVHE HKKCKGMKS 
      SSAEYWFLK EVSEFENFG EETFTVKNH QNNNTVYGI 
      GPHGITIYK KDGEKELIS FTNITSASS HKRTFKLEY 
      LTIDNEGAI LNIKLDSSH VASSLYRAI TEKHAFYSC 
      ETVRSAVTA QFIRDLKGT IVSIFNEDS TLGKKYVFD 
      IRRTCREVY DNARRALYL ENTIQEITR ANLDYNGNA 
      GECKNSQEK LSRFLDAMT CKICMDGQI DAIFLPCGH 
      GVACMACAS RCDRCPLCR SDIAQAKKV FLPIDYLLV 
      GQTTN
    CDS sequence
    • >TC008849 RA
      atgtggtgt ctcatcagc caggccaat tcggtcgtt 
      ttcgaagtc cgggtcgac cccaaagcc atcggccag 
      gaatgtctc gagaaggtg tgcgaattc gtgaatgtg 
      caatacgag tctgattac ttcggattg aagtacgaa 
      aaccacaag ggagaggaa tcctggtta aatttacga 
      aaccccatc gatcggcaa gtgactttt cacggccat 
      acgtcctat ctgcgctta tcactgcgg gtgaagttc 
      tgggttcct cctcacatc ttgcagcaa gaaagcacc 
      cgtcaccag ttttacctc aacgcgaga cttcatctg 
      ttggaaaaa cgcttaaca gccccggac tgggccagc 
      atttcaaga ctgatcgct tttatagct caagctgaa 
      tgtggcgac ttcaacgaa cttgatccg cctcggaat 
      atttacaat ttagctaca acgatcgct cctttggat 
      cagtcggag aaacccgga gatttgcta cagaggatc 
      gtccacgag cacaaaaaa tgcaaaggg atgaagagc 
      tcttccgcc gagtattgg tttttgaag gaagtgtcg 
      gagtttgag aatttcgga gaggagacg ttcacggtg 
      aaaaatcac cagaataac aacacggtg tacgggatc 
      ggccctcat ggcatcacg atttacaaa aaggacggg 
      gagaaggag ttgatttcg ttcactaac ataacgagt 
      gcttcctcc cataagcgc acttttaaa cttgaatat 
      ttgaccatc gataacgaa ggggctatt ctaaatatt 
      aaactagat tctagccat gtggctagt agcttatat 
      cgggccatt acggaaaaa cacgccttc tacagctgc 
      gagacggtt cgcagtgcc gtaacggca caatttatt 
      cgagatctg aaagggacc attgtttca attttcaac 
      gaggactcc acattgggc aaaaagtac gtgttcgat 
      attcggagg acctgcaga gaagtctac gacaatgca 
      agaagagcc ttgtatttg gaaaacact attcaggaa 
      atcactcgc gccaatctc gactacaac ggcaacgcg 
      ggagagtgc aagaattcc caagaaaaa ttgtcgaga 
      tttctggac gcaatgacc tgtaaaatc tgcatggac 
      ggccagatc gacgcgatt ttcctgccg tgcgggcac 
      ggggtggcc tgcatggcg tgcgcctcg cgctgcgac 
      aggtgcccc ctttgtcgt tccgacatc gcacaagcg 
      aagaaggtg tttttgccc attgactac ctcctggtg 
      ggtcagact acgaattga
    mRNA sequence
    • >TC008849 RA
      tgagtcagg tgcgttcgc tgttcgcgt gcgctgtca 
      ccccgtatg tctgagctg aaccaaata aatgtgaac 
      aaggaactt cgatttaat attgtccaa gctgttgca 
      tagtatgga cgttgtcgt aaaatgtgc aaagagttt 
      atacaacaa acgatacgc cgtcagtat atcagaaca 
      ttgcgattt tgtcaaatg atcagtggc ttctgctag 
      cgatttttc gtctagcca attttgagg ttcatttta 
      cgaattttt ggaaaatgt ggtgtctca tcagccagg 
      ccaattcgg tcgttttcg aagtccggg tcgacccca 
      aagccatcg gccaggaat gtctcgaga aggtgtgcg 
      aattcgtga atgtgcaat acgagtctg attacttcg 
      gattgaagt acgaaaacc acaagggag aggaatcct 
      ggttaaatt tacgaaacc ccatcgatc ggcaagtga 
      cttttcacg gccatacgt cctatctgc gcttatcac 
      tgcgggtga agttctggg ttcctcctc acatcttgc 
      agcaagaaa gcacccgtc accagtttt acctcaacg 
      cgagacttc atctgttgg aaaaacgct taacagccc 
      cggactggg ccagcattt caagactga tcgctttta 
      tagctcaag ctgaatgtg gcgacttca acgaacttg 
      atccgcctc ggaatattt acaatttag ctacaacga 
      tcgctcctt tggatcagt cggagaaac ccggagatt 
      tgctacaga ggatcgtcc acgagcaca aaaaatgca 
      aagggatga agagctctt ccgccgagt attggtttt 
      tgaaggaag tgtcggagt ttgagaatt tcggagagg 
      agacgttca cggtgaaaa atcaccaga ataacaaca 
      cggtgtacg ggatcggcc ctcatggca tcacgattt 
      acaaaaagg acggggaga aggagttga tttcgttca 
      ctaacataa cgagtgctt cctcccata agcgcactt 
      ttaaacttg aatatttga ccatcgata acgaagggg 
      ctattctaa atattaaac tagattcta gccatgtgg 
      ctagtagct tatatcggg ccattacgg aaaaacacg 
      ccttctaca gctgcgaga cggttcgca gtgccgtaa 
      cggcacaat ttattcgag atctgaaag ggaccattg 
      tttcaattt tcaacgagg actccacat tgggcaaaa 
      agtacgtgt tcgatattc ggaggacct gcagagaag 
      tctacgaca atgcaagaa gagccttgt atttggaaa 
      acactattc aggaaatca ctcgcgcca atctcgact 
      acaacggca acgcgggag agtgcaaga attcccaag 
      aaaaattgt cgagatttc tggacgcaa tgacctgta 
      aaatctgca tggacggcc agatcgacg cgattttcc 
      tgccgtgcg ggcacgggg tggcctgca tggcgtgcg 
      cctcgcgct gcgacaggt gcccccttt gtcgttccg 
      acatcgcac aagcgaaga aggtgtttt tgcccattg 
      actacctcc tggtgggtc agactacga attgaaaga 
      ctgcatttt tactttact aagatctcg aatagttta 
      gttgtggaa gtggacaga gtacacaag tgacaatcg 
      tataatttt tgatctttt cttgtgatg ttttgttaa 
      ccaaaatct catattgag gtttgtttt gtacattgt 
      taagtatct atctacata ctatttttt actatgtaa 
      agtatgaag gacctaaag tgttgtatt ttattaact 
      attgacaat agtaagaaa atgcaaaag ctatcgaaa 
      tgtaaatga catgaatta ttaatagtc atattatca 
      atgcaagat gtaaattag gtttagttt cgatatttg 
      ttaatataa taaaaatgt tataatgct agtattata 
      atgagaaat gtgtgataa gaaattttt gttttgtga 
      tattgcatt catttggaa aattaagtt tacgtttat 
      actatcaaa ttttattaa tcgatgtta aagtaaagt 
      gtagacgtg tatatgtca catatttac aagtatttt 
      gttattagt attataacg agtatatct gttaaattt 
      tttgctatt cacgtttac gtcaacatg gaattacaa 
      taattattt atgttgttt tatttcaaa aaaaacctt 
      ccttattta ctaataaag gtaagtgga attg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY