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Gene information
General information
TC008834 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid49: 3922450..3926646
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008834
    iBeetle screen iB_01425
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008834 RA
      MWSFFSRDP SKDFNFEVG ELVKGLDER SVWTLHKGK 
      RKGTNEEVS VFVFDIKGG SETQLEIAK AAVKRLKTL 
      RHPSILTYL DSYESDKVL YLATEHVEP LASHLEKLT 
      IEGAQKDLY IAWGLFQIT RALSFLNND GNLRHNNVN 
      IWSVFVNSS GEWKLGGVE YVSSAQDNS NVTIKIIPS 
      LEMYDPPEK NDPTKQRSI TKCSADMWG LGCLIWEVF 
      NGPLYQQSS LKLLDKIPK QLGPLYCEL VGANPSSRP 
      NPADIITRC RKLGGYFKN DLVDTLLFL EEIQIKDKN 
      EKNRFFSNL TPHLDNFPE SVCKHKILP QLITAFDYG 
      DAGSAVLAP MFKLGQLLD EAEYQKKIV PCVVKLFVS 
      NDRATRSRL LQQLEHFIG HLQAATVNE QIFPQIAHG 
      FMDTNPTIR EQTVKSVIH LAPKLSYNN LNVEVLRHF 
      ARLQSKDDQ GGIRTNTTV CLGKIAQHL HPQIRQKVL 
      ISAFIRAMR DPFPPARNA GILALAATQ QYYLLNEVS 
      SRILPALCQ LTMDPEKSV RDSVFRTIK GFLGKLEKV 
      SEDPSLRES MEADVNTAT PSLSNAAAT WAGWAVTAV 
      TAKFYRSQS DTVKSMNPM ASKMLTKPA SLEQPSSSS 
      ISTTTSSVT SMTSLEHEE GRSGTNKIT DGWDNEEWG 
      SLEEEPEVE AELTSPSEA WSPSDTTPI IPESYEHGL 
      SNRKSANNW GDSEFEPLE EQPSTTNPK LEEAKKKRE 
      ERKLMRQKE LEARRASRT TGGPMKLGA KKL
    CDS sequence
    • >TC008834 RA
      atgtggtca tttttctcc cgcgatcct tccaaagac 
      ttcaacttt gaagtcgga gagttagtc aaggggctt 
      gacgaacgg agtgtatgg actttacat aagggcaaa 
      agaaagggc acaaatgag gaagtttca gtgtttgtt 
      tttgatata aagggtgga tcagaaaca cagttagaa 
      attgccaaa gcggcagtt aaacggcta aaaacattg 
      agacaccct agtatttta acttattta gatagttac 
      gaatcggat aaggttttg tatttagcg acagaacac 
      gttgaaccg ttggctagt cacttagag aagttgacg 
      atagaaggg gctcaaaag gatttgtat attgcctgg 
      gggctgttc caaatcacg cgagctttg tctttctta 
      aataatgac ggtaatttg agacacaac aatgttaac 
      atttggtca gtttttgtc aattcttcc ggtgagtgg 
      aagctggga ggtgttgaa tatgtctca agtgctcaa 
      gacaactcc aatgttact attaaaatc ataccatcg 
      ttggaaatg tacgaccct cctgaaaaa aatgaccca 
      actaaacag cggtcaatt acgaaatgt tctgccgat 
      atgtggggg ctgggttgt ctaatatgg gaagttttc 
      aatggtcct ttatatcaa cagtcttct ttgaaactc 
      ctagacaag attcctaaa cagttgggt ccattatat 
      tgcgagtta gttggagcg aatccgtca tcaaggcct 
      aatcctgcc gatattata actagatgc cgaaagctc 
      ggcggctac tttaaaaac gatcttgta gacacttta 
      ctattttta gaagagatt caaattaag gacaaaaac 
      gagaaaaac cgatttttc tcaaaccta acaccacac 
      ttagacaac ttccccgaa agcgtgtgt aagcacaag 
      attttgccc cagttgata acagccttt gactacggg 
      gacgccgga tctgcagtt ctagccccc atgtttaag 
      ttgggccaa ctccttgac gaagccgaa taccagaaa 
      aaaatcgtg ccctgcgtg gtcaaacta ttcgtaagc 
      aacgacaga gcaacaagg tcccgactt ttgcaacaa 
      cttgaacac ttcatcggt catttgcaa gctgcgacc 
      gtaaacgaa caaattttc cctcaaatt gctcacggg 
      tttatggat accaacccc acaattcgc gaacagact 
      gtcaaatct gtgatacat ctagcacct aaattaagt 
      tacaacaat ttaaacgtg gaggttttg cggcatttt 
      gcgcggctc cagtcgaag gacgatcaa gggggcatt 
      cgcaccaac actacggtg tgtctgggg aagatagca 
      caacatttg catccgcaa attaggcaa aaagtcctc 
      atttcggcg tttatacga gcgatgagg gatcccttt 
      cctccggca cgtaacgca ggcattttg gccttggct 
      gcgacgcaa cagtattat cttttgaac gaagtttcg 
      tcgagaatt ttgccggca ttgtgtcag ttaacgatg 
      gatccggaa aagtcggtc cgggattcg gtttttagg 
      acgattaaa gggtttttg gggaagttg gagaaggtg 
      tcagaggat cccagcttg agggagagt atggaggcc 
      gatgtcaat actgcaacc ccgagtcta agcaacgca 
      gccgccacg tgggctggg tgggctgtt acagcagtt 
      acagcgaaa ttctacaga agtcaatca gatacagtc 
      aaatcaatg aatcctatg gccagtaaa atgttaact 
      aaaccggct tcactagaa caaccatcg agtagcagc 
      atttcaacg accacttct agtgttact tctatgacg 
      tctttagaa cacgaggag ggaaggtct ggtactaac 
      aaaattaca gacggttgg gataacgaa gaatggggc 
      agtcttgaa gaagagccg gaggtcgaa gctgaactt 
      acaagccct tcagaagcg tggagcccc agtgatacg 
      accccgata atccccgaa tcgtacgaa cacggtttg 
      agtaacagg aaatcggct aataattgg ggcgattct 
      gagtttgaa ccgttagaa gagcaacca tctaccact 
      aatccaaag ttagaagaa gccaaaaag aagcgagaa 
      gagaggaaa ttaatgcgg caaaaggag ttagaagca 
      cggcgggcc agtcggaca acgggtgga ccaatgaaa 
      ctgggggct aaaaaatta taa
    mRNA sequence
    • >TC008834 RA
      taggttaga tttttaaaa ctttgtaca aatttcgta 
      taattcagc ataaatcca gcatataat cgctaagct 
      agtgtcatg tggtcattt ttctcccgc gatccttcc 
      aaagacttc aactttgaa gtcggagag ttagtcaag 
      gggcttgac gaacggagt gtatggact ttacataag 
      ggcaaaaga aagggcaca aatgaggaa gtttcagtg 
      tttgttttt gatataaag ggtggatca gaaacacag 
      ttagaaatt gccaaagcg gcagttaaa cggctaaaa 
      acattgaga caccctagt attttaact tatttagat 
      agttacgaa tcggataag gttttgtat ttagcgaca 
      gaacacgtt gaaccgttg gctagtcac ttagagaag 
      ttgacgata gaaggggct caaaaggat ttgtatatt 
      gcctggggg ctgttccaa atcacgcga gctttgtct 
      ttcttaaat aatgacggt aatttgaga cacaacaat 
      gttaacatt tggtcagtt tttgtcaat tcttccggt 
      gagtggaag ctgggaggt gttgaatat gtctcaagt 
      gctcaagac aactccaat gttactatt aaaatcata 
      ccatcgttg gaaatgtac gaccctcct gaaaaaaat 
      gacccaact aaacagcgg tcaattacg aaatgttct 
      gccgatatg tgggggctg ggttgtcta atatgggaa 
      gttttcaat ggtccttta tatcaacag tcttctttg 
      aaactccta gacaagatt cctaaacag ttgggtcca 
      ttatattgc gagttagtt ggagcgaat ccgtcatca 
      aggcctaat cctgccgat attataact agatgccga 
      aagctcggc ggctacttt aaaaacgat cttgtagac 
      actttacta tttttagaa gagattcaa attaaggac 
      aaaaacgag aaaaaccga tttttctca aacctaaca 
      ccacactta gacaacttc cccgaaagc gtgtgtaag 
      cacaagatt ttgccccag ttgataaca gcctttgac 
      tacggggac gccggatct gcagttcta gcccccatg 
      tttaagttg ggccaactc cttgacgaa gccgaatac 
      cagaaaaaa atcgtgccc tgcgtggtc aaactattc 
      gtaagcaac gacagagca acaaggtcc cgacttttg 
      caacaactt gaacacttc atcggtcat ttgcaagct 
      gcgaccgta aacgaacaa attttccct caaattgct 
      cacgggttt atggatacc aaccccaca attcgcgaa 
      cagactgtc aaatctgtg atacatcta gcacctaaa 
      ttaagttac aacaattta aacgtggag gttttgcgg 
      cattttgcg cggctccag tcgaaggac gatcaaggg 
      ggcattcgc accaacact acggtgtgt ctggggaag 
      atagcacaa catttgcat ccgcaaatt aggcaaaaa 
      gtcctcatt tcggcgttt atacgagcg atgagggat 
      ccctttcct ccggcacgt aacgcaggc attttggcc 
      ttggctgcg acgcaacag tattatctt ttgaacgaa 
      gtttcgtcg agaattttg ccggcattg tgtcagtta 
      acgatggat ccggaaaag tcggtccgg gattcggtt 
      tttaggacg attaaaggg tttttgggg aagttggag 
      aaggtgtca gaggatccc agcttgagg gagagtatg 
      gaggccgat gtcaatact gcaaccccg agtctaagc 
      aacgcagcc gccacgtgg gctgggtgg gctgttaca 
      gcagttaca gcgaaattc tacagaagt caatcagat 
      acagtcaaa tcaatgaat cctatggcc agtaaaatg 
      ttaactaaa ccggcttca ctagaacaa ccatcgagt 
      agcagcatt tcaacgacc acttctagt gttacttct 
      atgacgtct ttagaacac gaggaggga aggtctggt 
      actaacaaa attacagac ggttgggat aacgaagaa 
      tggggcagt cttgaagaa gagccggag gtcgaagct 
      gaacttaca agcccttca gaagcgtgg agccccagt 
      gatacgacc ccgataatc cccgaatcg tacgaacac 
      ggtttgagt aacaggaaa tcggctaat aattggggc 
      gattctgag tttgaaccg ttagaagag caaccatct 
      accactaat ccaaagtta gaagaagcc aaaaagaag 
      cgagaagag aggaaatta atgcggcaa aaggagtta 
      gaagcacgg cgggccagt cggacaacg ggtggacca 
      atgaaactg ggggctaaa aaattataa atgtcgaat 
      taaatggaa ttaaacaga cttaaatat acaccaaac 
      ttgatacag acatatctt gaagcattt tgtcccaat 
      tttgattag gaatagtgg aaccggcat aattatact 
      gaggataat tagtgtgtt aatgtagtc taattattg 
      atttggtct tgtttttct acggaattc taaacgtgt 
      accaacaat aacatgctt tttgtgtac ataatatgc 
      aatttttaa agtataaaa acacgatac cgacgatgt 
      gaataatgg tttcgtttc tgtcgcggt tcttcctcg 
      gctttttat atcaaataa ttgtaaaca tttgcatgc 
      tggttgtat ttatgtaaa taattttac agatgtttt 
      aatatgtat gatattgtt taaataact ttatttctg 
      tatattttt ctttactat ttgcgttaa ctgaattat 
      atttttcta tgtatgtat tacattacc agaataaat 
      ttgtttcaa gcat