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Gene information
General information
TC008834 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10589797..10593993
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC008834
    iBeetle screen iB_01425

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008834 RA
      MWSFFSRDP SKDFNFEVG ELVKGLDER SVWTLHKGK 
RKGTNEEVS VFVFDIKGG SETQLEIAK AAVKRLKTL 
RHPSILTYL DSYESDKVL YLATEHVEP LASHLEKLT 
IEGAQKDLY IAWGLFQIT RALSFLNND GNLRHNNVN 
IWSVFVNSS GEWKLGGVE YVSSAQDNS NVTIKIIPS 
LEMYDPPEK NDPTKQRSI TKCSADMWG LGCLIWEVF 
NGPLYQQSS LKLLDKIPK QLGPLYCEL VGANPSSRP 
NPADIITRC RKLGGYFKN DLVDTLLFL EEIQIKDKN 
EKNRFFSNL TPHLDNFPE SVCKHKILP QLITAFDYG 
DAGSAVLAP MFKLGQLLD EAEYQKKIV PCVVKLFVS 
NDRATRSRL LQQLEHFIG HLQAATVNE QIFPQIAHG 
FMDTNPTIR EQTVKSVIH LAPKLSYNN LNVEVLRHF 
ARLQSKDDQ GGIRTNTTV CLGKIAQHL HPQIRQKVL 
ISAFIRAMR DPFPPARNA GILALAATQ QYYLLNEVS 
SRILPALCQ LTMDPEKSV RDSVFRTIK GFLGKLEKV 
SEDPSLRES MEADVNTAT PSLSNAAAT WAGWAVTAV 
TAKFYRSQS DTVKSMNPM ASKMLTKPA SLEQPSSSS 
ISTTTSSVT SMTSLEHEE GRSGTNKIT DGWDNEEWG 
SLEEEPEVE AELTSPSEA WSPSDTTPI IPESYEHGL 
SNRKSANNW GDSEFEPLE EQPSTTNPK LEEAKKKRE 
ERKLMRQKE LEARRASRT TGGPMKLGA KKL
    CDS sequence
    • >TC008834 RA
      atgtggtca tttttctcc cgcgatcct tccaaagac 
ttcaacttt gaagtcgga gagttagtc aaggggctt 
gacgaacgg agtgtatgg actttacat aagggcaaa 
agaaagggc acaaatgag gaagtttca gtgtttgtt 
tttgatata aagggtgga tcagaaaca cagttagaa 
attgccaaa gcggcagtt aaacggcta aaaacattg 
agacaccct agtatttta acttattta gatagttac 
gaatcggat aaggttttg tatttagcg acagaacac 
gttgaaccg ttggctagt cacttagag aagttgacg 
atagaaggg gctcaaaag gatttgtat attgcctgg 
gggctgttc caaatcacg cgagctttg tctttctta 
aataatgac ggtaatttg agacacaac aatgttaac 
atttggtca gtttttgtc aattcttcc ggtgagtgg 
aagctggga ggtgttgaa tatgtctca agtgctcaa 
gacaactcc aatgttact attaaaatc ataccatcg 
ttggaaatg tacgaccct cctgaaaaa aatgaccca 
actaaacag cggtcaatt acgaaatgt tctgccgat 
atgtggggg ctgggttgt ctaatatgg gaagttttc 
aatggtcct ttatatcaa cagtcttct ttgaaactc 
ctagacaag attcctaaa cagttgggt ccattatat 
tgcgagtta gttggagcg aatccgtca tcaaggcct 
aatcctgcc gatattata actagatgc cgaaagctc 
ggcggctac tttaaaaac gatcttgta gacacttta 
ctattttta gaagagatt caaattaag gacaaaaac 
gagaaaaac cgatttttc tcaaaccta acaccacac 
ttagacaac ttccccgaa agcgtgtgt aagcacaag 
attttgccc cagttgata acagccttt gactacggg 
gacgccgga tctgcagtt ctagccccc atgtttaag 
ttgggccaa ctccttgac gaagccgaa taccagaaa 
aaaatcgtg ccctgcgtg gtcaaacta ttcgtaagc 
aacgacaga gcaacaagg tcccgactt ttgcaacaa 
cttgaacac ttcatcggt catttgcaa gctgcgacc 
gtaaacgaa caaattttc cctcaaatt gctcacggg 
tttatggat accaacccc acaattcgc gaacagact 
gtcaaatct gtgatacat ctagcacct aaattaagt 
tacaacaat ttaaacgtg gaggttttg cggcatttt 
gcgcggctc cagtcgaag gacgatcaa gggggcatt 
cgcaccaac actacggtg tgtctgggg aagatagca 
caacatttg catccgcaa attaggcaa aaagtcctc 
atttcggcg tttatacga gcgatgagg gatcccttt 
cctccggca cgtaacgca ggcattttg gccttggct 
gcgacgcaa cagtattat cttttgaac gaagtttcg 
tcgagaatt ttgccggca ttgtgtcag ttaacgatg 
gatccggaa aagtcggtc cgggattcg gtttttagg 
acgattaaa gggtttttg gggaagttg gagaaggtg 
tcagaggat cccagcttg agggagagt atggaggcc 
gatgtcaat actgcaacc ccgagtcta agcaacgca 
gccgccacg tgggctggg tgggctgtt acagcagtt 
acagcgaaa ttctacaga agtcaatca gatacagtc 
aaatcaatg aatcctatg gccagtaaa atgttaact 
aaaccggct tcactagaa caaccatcg agtagcagc 
atttcaacg accacttct agtgttact tctatgacg 
tctttagaa cacgaggag ggaaggtct ggtactaac 
aaaattaca gacggttgg gataacgaa gaatggggc 
agtcttgaa gaagagccg gaggtcgaa gctgaactt 
acaagccct tcagaagcg tggagcccc agtgatacg 
accccgata atccccgaa tcgtacgaa cacggtttg 
agtaacagg aaatcggct aataattgg ggcgattct 
gagtttgaa ccgttagaa gagcaacca tctaccact 
aatccaaag ttagaagaa gccaaaaag aagcgagaa 
gagaggaaa ttaatgcgg caaaaggag ttagaagca 
cggcgggcc agtcggaca acgggtgga ccaatgaaa 
ctgggggct aaaaaatta taa
    mRNA sequence
    • >TC008834 RA
      taggttaga tttttaaaa ctttgtaca aatttcgta 
taattcagc ataaatcca gcatataat cgctaagct 
agtgtcatg tggtcattt ttctcccgc gatccttcc 
aaagacttc aactttgaa gtcggagag ttagtcaag 
gggcttgac gaacggagt gtatggact ttacataag 
ggcaaaaga aagggcaca aatgaggaa gtttcagtg 
tttgttttt gatataaag ggtggatca gaaacacag 
ttagaaatt gccaaagcg gcagttaaa cggctaaaa 
acattgaga caccctagt attttaact tatttagat 
agttacgaa tcggataag gttttgtat ttagcgaca 
gaacacgtt gaaccgttg gctagtcac ttagagaag 
ttgacgata gaaggggct caaaaggat ttgtatatt 
gcctggggg ctgttccaa atcacgcga gctttgtct 
ttcttaaat aatgacggt aatttgaga cacaacaat 
gttaacatt tggtcagtt tttgtcaat tcttccggt 
gagtggaag ctgggaggt gttgaatat gtctcaagt 
gctcaagac aactccaat gttactatt aaaatcata 
ccatcgttg gaaatgtac gaccctcct gaaaaaaat 
gacccaact aaacagcgg tcaattacg aaatgttct 
gccgatatg tgggggctg ggttgtcta atatgggaa 
gttttcaat ggtccttta tatcaacag tcttctttg 
aaactccta gacaagatt cctaaacag ttgggtcca 
ttatattgc gagttagtt ggagcgaat ccgtcatca 
aggcctaat cctgccgat attataact agatgccga 
aagctcggc ggctacttt aaaaacgat cttgtagac 
actttacta tttttagaa gagattcaa attaaggac 
aaaaacgag aaaaaccga tttttctca aacctaaca 
ccacactta gacaacttc cccgaaagc gtgtgtaag 
cacaagatt ttgccccag ttgataaca gcctttgac 
tacggggac gccggatct gcagttcta gcccccatg 
tttaagttg ggccaactc cttgacgaa gccgaatac 
cagaaaaaa atcgtgccc tgcgtggtc aaactattc 
gtaagcaac gacagagca acaaggtcc cgacttttg 
caacaactt gaacacttc atcggtcat ttgcaagct 
gcgaccgta aacgaacaa attttccct caaattgct 
cacgggttt atggatacc aaccccaca attcgcgaa 
cagactgtc aaatctgtg atacatcta gcacctaaa 
ttaagttac aacaattta aacgtggag gttttgcgg 
cattttgcg cggctccag tcgaaggac gatcaaggg 
ggcattcgc accaacact acggtgtgt ctggggaag 
atagcacaa catttgcat ccgcaaatt aggcaaaaa 
gtcctcatt tcggcgttt atacgagcg atgagggat 
ccctttcct ccggcacgt aacgcaggc attttggcc 
ttggctgcg acgcaacag tattatctt ttgaacgaa 
gtttcgtcg agaattttg ccggcattg tgtcagtta 
acgatggat ccggaaaag tcggtccgg gattcggtt 
tttaggacg attaaaggg tttttgggg aagttggag 
aaggtgtca gaggatccc agcttgagg gagagtatg 
gaggccgat gtcaatact gcaaccccg agtctaagc 
aacgcagcc gccacgtgg gctgggtgg gctgttaca 
gcagttaca gcgaaattc tacagaagt caatcagat 
acagtcaaa tcaatgaat cctatggcc agtaaaatg 
ttaactaaa ccggcttca ctagaacaa ccatcgagt 
agcagcatt tcaacgacc acttctagt gttacttct 
atgacgtct ttagaacac gaggaggga aggtctggt 
actaacaaa attacagac ggttgggat aacgaagaa 
tggggcagt cttgaagaa gagccggag gtcgaagct 
gaacttaca agcccttca gaagcgtgg agccccagt 
gatacgacc ccgataatc cccgaatcg tacgaacac 
ggtttgagt aacaggaaa tcggctaat aattggggc 
gattctgag tttgaaccg ttagaagag caaccatct 
accactaat ccaaagtta gaagaagcc aaaaagaag 
cgagaagag aggaaatta atgcggcaa aaggagtta 
gaagcacgg cgggccagt cggacaacg ggtggacca 
atgaaactg ggggctaaa aaattataa atgtcgaat 
taaatggaa ttaaacaga cttaaatat acaccaaac 
ttgatacag acatatctt gaagcattt tgtcccaat 
tttgattag gaatagtgg aaccggcat aattatact 
gaggataat tagtgtgtt aatgtagtc taattattg 
atttggtct tgtttttct acggaattc taaacgtgt 
accaacaat aacatgctt tttgtgtac ataatatgc 
aatttttaa agtataaaa acacgatac cgacgatgt 
gaataatgg tttcgtttc tgtcgcggt tcttcctcg 
gctttttat atcaaataa ttgtaaaca tttgcatgc 
tggttgtat ttatgtaaa taattttac agatgtttt 
aatatgtat gatattgtt taaataact ttatttctg 
tatattttt ctttactat ttgcgttaa ctgaattat 
atttttcta tgtatgtat tacattacc agaataaat 
ttgtttcaa gcat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY