| General information | |||
| TC008834 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 10589797..10593993 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC008834 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01425 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
- >TC008834 RA
MWSFFSRDP SKDFNFEVG ELVKGLDER SVWTLHKGK RKGTNEEVS VFVFDIKGG SETQLEIAK AAVKRLKTL RHPSILTYL DSYESDKVL YLATEHVEP LASHLEKLT IEGAQKDLY IAWGLFQIT RALSFLNND GNLRHNNVN IWSVFVNSS GEWKLGGVE YVSSAQDNS NVTIKIIPS LEMYDPPEK NDPTKQRSI TKCSADMWG LGCLIWEVF NGPLYQQSS LKLLDKIPK QLGPLYCEL VGANPSSRP NPADIITRC RKLGGYFKN DLVDTLLFL EEIQIKDKN EKNRFFSNL TPHLDNFPE SVCKHKILP QLITAFDYG DAGSAVLAP MFKLGQLLD EAEYQKKIV PCVVKLFVS NDRATRSRL LQQLEHFIG HLQAATVNE QIFPQIAHG FMDTNPTIR EQTVKSVIH LAPKLSYNN LNVEVLRHF ARLQSKDDQ GGIRTNTTV CLGKIAQHL HPQIRQKVL ISAFIRAMR DPFPPARNA GILALAATQ QYYLLNEVS SRILPALCQ LTMDPEKSV RDSVFRTIK GFLGKLEKV SEDPSLRES MEADVNTAT PSLSNAAAT WAGWAVTAV TAKFYRSQS DTVKSMNPM ASKMLTKPA SLEQPSSSS ISTTTSSVT SMTSLEHEE GRSGTNKIT DGWDNEEWG SLEEEPEVE AELTSPSEA WSPSDTTPI IPESYEHGL SNRKSANNW GDSEFEPLE EQPSTTNPK LEEAKKKRE ERKLMRQKE LEARRASRT TGGPMKLGA KKL
- >TC008834 RA
atgtggtca tttttctcc cgcgatcct tccaaagac ttcaacttt gaagtcgga gagttagtc aaggggctt gacgaacgg agtgtatgg actttacat aagggcaaa agaaagggc acaaatgag gaagtttca gtgtttgtt tttgatata aagggtgga tcagaaaca cagttagaa attgccaaa gcggcagtt aaacggcta aaaacattg agacaccct agtatttta acttattta gatagttac gaatcggat aaggttttg tatttagcg acagaacac gttgaaccg ttggctagt cacttagag aagttgacg atagaaggg gctcaaaag gatttgtat attgcctgg gggctgttc caaatcacg cgagctttg tctttctta aataatgac ggtaatttg agacacaac aatgttaac atttggtca gtttttgtc aattcttcc ggtgagtgg aagctggga ggtgttgaa tatgtctca agtgctcaa gacaactcc aatgttact attaaaatc ataccatcg ttggaaatg tacgaccct cctgaaaaa aatgaccca actaaacag cggtcaatt acgaaatgt tctgccgat atgtggggg ctgggttgt ctaatatgg gaagttttc aatggtcct ttatatcaa cagtcttct ttgaaactc ctagacaag attcctaaa cagttgggt ccattatat tgcgagtta gttggagcg aatccgtca tcaaggcct aatcctgcc gatattata actagatgc cgaaagctc ggcggctac tttaaaaac gatcttgta gacacttta ctattttta gaagagatt caaattaag gacaaaaac gagaaaaac cgatttttc tcaaaccta acaccacac ttagacaac ttccccgaa agcgtgtgt aagcacaag attttgccc cagttgata acagccttt gactacggg gacgccgga tctgcagtt ctagccccc atgtttaag ttgggccaa ctccttgac gaagccgaa taccagaaa aaaatcgtg ccctgcgtg gtcaaacta ttcgtaagc aacgacaga gcaacaagg tcccgactt ttgcaacaa cttgaacac ttcatcggt catttgcaa gctgcgacc gtaaacgaa caaattttc cctcaaatt gctcacggg tttatggat accaacccc acaattcgc gaacagact gtcaaatct gtgatacat ctagcacct aaattaagt tacaacaat ttaaacgtg gaggttttg cggcatttt gcgcggctc cagtcgaag gacgatcaa gggggcatt cgcaccaac actacggtg tgtctgggg aagatagca caacatttg catccgcaa attaggcaa aaagtcctc atttcggcg tttatacga gcgatgagg gatcccttt cctccggca cgtaacgca ggcattttg gccttggct gcgacgcaa cagtattat cttttgaac gaagtttcg tcgagaatt ttgccggca ttgtgtcag ttaacgatg gatccggaa aagtcggtc cgggattcg gtttttagg acgattaaa gggtttttg gggaagttg gagaaggtg tcagaggat cccagcttg agggagagt atggaggcc gatgtcaat actgcaacc ccgagtcta agcaacgca gccgccacg tgggctggg tgggctgtt acagcagtt acagcgaaa ttctacaga agtcaatca gatacagtc aaatcaatg aatcctatg gccagtaaa atgttaact aaaccggct tcactagaa caaccatcg agtagcagc atttcaacg accacttct agtgttact tctatgacg tctttagaa cacgaggag ggaaggtct ggtactaac aaaattaca gacggttgg gataacgaa gaatggggc agtcttgaa gaagagccg gaggtcgaa gctgaactt acaagccct tcagaagcg tggagcccc agtgatacg accccgata atccccgaa tcgtacgaa cacggtttg agtaacagg aaatcggct aataattgg ggcgattct gagtttgaa ccgttagaa gagcaacca tctaccact aatccaaag ttagaagaa gccaaaaag aagcgagaa gagaggaaa ttaatgcgg caaaaggag ttagaagca cggcgggcc agtcggaca acgggtgga ccaatgaaa ctgggggct aaaaaatta taa
- >TC008834 RA
taggttaga tttttaaaa ctttgtaca aatttcgta taattcagc ataaatcca gcatataat cgctaagct agtgtcatg tggtcattt ttctcccgc gatccttcc aaagacttc aactttgaa gtcggagag ttagtcaag gggcttgac gaacggagt gtatggact ttacataag ggcaaaaga aagggcaca aatgaggaa gtttcagtg tttgttttt gatataaag ggtggatca gaaacacag ttagaaatt gccaaagcg gcagttaaa cggctaaaa acattgaga caccctagt attttaact tatttagat agttacgaa tcggataag gttttgtat ttagcgaca gaacacgtt gaaccgttg gctagtcac ttagagaag ttgacgata gaaggggct caaaaggat ttgtatatt gcctggggg ctgttccaa atcacgcga gctttgtct ttcttaaat aatgacggt aatttgaga cacaacaat gttaacatt tggtcagtt tttgtcaat tcttccggt gagtggaag ctgggaggt gttgaatat gtctcaagt gctcaagac aactccaat gttactatt aaaatcata ccatcgttg gaaatgtac gaccctcct gaaaaaaat gacccaact aaacagcgg tcaattacg aaatgttct gccgatatg tgggggctg ggttgtcta atatgggaa gttttcaat ggtccttta tatcaacag tcttctttg aaactccta gacaagatt cctaaacag ttgggtcca ttatattgc gagttagtt ggagcgaat ccgtcatca aggcctaat cctgccgat attataact agatgccga aagctcggc ggctacttt aaaaacgat cttgtagac actttacta tttttagaa gagattcaa attaaggac aaaaacgag aaaaaccga tttttctca aacctaaca ccacactta gacaacttc cccgaaagc gtgtgtaag cacaagatt ttgccccag ttgataaca gcctttgac tacggggac gccggatct gcagttcta gcccccatg tttaagttg ggccaactc cttgacgaa gccgaatac cagaaaaaa atcgtgccc tgcgtggtc aaactattc gtaagcaac gacagagca acaaggtcc cgacttttg caacaactt gaacacttc atcggtcat ttgcaagct gcgaccgta aacgaacaa attttccct caaattgct cacgggttt atggatacc aaccccaca attcgcgaa cagactgtc aaatctgtg atacatcta gcacctaaa ttaagttac aacaattta aacgtggag gttttgcgg cattttgcg cggctccag tcgaaggac gatcaaggg ggcattcgc accaacact acggtgtgt ctggggaag atagcacaa catttgcat ccgcaaatt aggcaaaaa gtcctcatt tcggcgttt atacgagcg atgagggat ccctttcct ccggcacgt aacgcaggc attttggcc ttggctgcg acgcaacag tattatctt ttgaacgaa gtttcgtcg agaattttg ccggcattg tgtcagtta acgatggat ccggaaaag tcggtccgg gattcggtt tttaggacg attaaaggg tttttgggg aagttggag aaggtgtca gaggatccc agcttgagg gagagtatg gaggccgat gtcaatact gcaaccccg agtctaagc aacgcagcc gccacgtgg gctgggtgg gctgttaca gcagttaca gcgaaattc tacagaagt caatcagat acagtcaaa tcaatgaat cctatggcc agtaaaatg ttaactaaa ccggcttca ctagaacaa ccatcgagt agcagcatt tcaacgacc acttctagt gttacttct atgacgtct ttagaacac gaggaggga aggtctggt actaacaaa attacagac ggttgggat aacgaagaa tggggcagt cttgaagaa gagccggag gtcgaagct gaacttaca agcccttca gaagcgtgg agccccagt gatacgacc ccgataatc cccgaatcg tacgaacac ggtttgagt aacaggaaa tcggctaat aattggggc gattctgag tttgaaccg ttagaagag caaccatct accactaat ccaaagtta gaagaagcc aaaaagaag cgagaagag aggaaatta atgcggcaa aaggagtta gaagcacgg cgggccagt cggacaacg ggtggacca atgaaactg ggggctaaa aaattataa atgtcgaat taaatggaa ttaaacaga cttaaatat acaccaaac ttgatacag acatatctt gaagcattt tgtcccaat tttgattag gaatagtgg aaccggcat aattatact gaggataat tagtgtgtt aatgtagtc taattattg atttggtct tgtttttct acggaattc taaacgtgt accaacaat aacatgctt tttgtgtac ataatatgc aatttttaa agtataaaa acacgatac cgacgatgt gaataatgg tttcgtttc tgtcgcggt tcttcctcg gctttttat atcaaataa ttgtaaaca tttgcatgc tggttgtat ttatgtaaa taattttac agatgtttt aatatgtat gatattgtt taaataact ttatttctg tatattttt ctttactat ttgcgttaa ctgaattat atttttcta tgtatgtat tacattacc agaataaat ttgtttcaa gcat
