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Gene information
General information
TC008825 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10496389..10497848
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC008825
      iBeetle screen iB_01422

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

      embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%

      cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): less than 30%

      cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC008825 RA
        MNSTGNVLQ PTHGGLHCS KDALTDVFG WFLQVLLAC 
        LAFTCLIAK RFCEPRSER RSWLIWFYD TSKQGMGAM 
        AIHLANVWL AGQYQGDPC TWYLINFLL DSSLGLLII 
        FVGIRLSQY LSRKKGWEA INFGEYGKP PSANAWMAQ 
        CCLYVGLMA IVKVSITLF MQLDFWDGV KDFILSPIN 
        NPKVELAFV MLIIPFFVN MLMFWVTDN FLMHKSHSK 
        RQARRTSAE NSLLARTKV KYRTIKKKI RRRDSESDI 
        LLSGDDELL DPESVPINR HVLT
      CDS sequence
      • >TC008825 RA
        atgaattcg acaggaaac gtgctacag cccacccat 
        gggggcttg cattgcagc aaagatgcc ctaactgac 
        gttttcggc tggtttctg caagtctta ctggcctgt 
        ttagccttc acctgcctc atagctaaa aggttctgt 
        gagccaagg agcgagcgg cgttcttgg ttgatttgg 
        ttctatgac acttcaaag caaggtatg ggggctatg 
        gccattcac ctggccaac gtatggctc gcggggcag 
        taccaaggc gatccgtgc acctggtac ttgatcaat 
        ttcctcctg gactcctcc ttggggctg cttattatt 
        ttcgtaggg attcgttta agtcaatat ttatcgaga 
        aagaaaggc tgggaagcg attaatttt ggggagtat 
        gggaagccg ccttcggca aatgcgtgg atggcgcag 
        tgttgtctt tacgtgggc cttatggcc attgttaaa 
        gtgtcaata acgttgttt atgcagctt gatttttgg 
        gatggcgtt aaagatttt attttatcg cctattaat 
        aatcccaag gtggagctg gcgtttgtt atgctcatc 
        ataccgttt ttcgtaaac atgttgatg ttttgggtc 
        actgataac tttctcatg cataaaagt cacagcaaa 
        aggcaagcg agaaggacg agcgctgaa aactcactt 
        ttggcgcgc accaaagtc aagtatcga acaattaag 
        aaaaaaatt agaagacgc gactctgaa agcgatatt 
        ctgctttca ggagatgat gaattgttg gatcctgag 
        tcagtacct ataaataga cacgtgtta acttaa
      mRNA sequence
      • >TC008825 RA
        aattaaaca tttacaaaa accaagaat gataattga 
        taatagata gtgaagtgt tgtcagtgc tatgaattc 
        gacaggaaa cgtgctaca gcccaccca tgggggctt 
        gcattgcag caaagatgc cctaactga cgttttcgg 
        ctggtttct gcaagtctt actggcctg tttagcctt 
        cacctgcct catagctaa aaggttctg tgagccaag 
        gagcgagcg gcgttcttg gttgatttg gttctatga 
        cacttcaaa gcaaggtat gggggctat ggccattca 
        cctggccaa cgtatggct cgcggggca gtaccaagg 
        cgatccgtg cacctggta cttgatcaa tttcctcct 
        ggactcctc cttggggct gcttattat tttcgtagg 
        gattcgttt aagtcaata tttatcgag aaagaaagg 
        ctgggaagc gattaattt tggggagta tgggaagcc 
        gccttcggc aaatgcgtg gatggcgca gtgttgtct 
        ttacgtggg ccttatggc cattgttaa agtgtcaat 
        aacgttgtt tatgcagct tgatttttg ggatggcgt 
        taaagattt tattttatc gcctattaa taatcccaa 
        ggtggagct ggcgtttgt tatgctcat cataccgtt 
        tttcgtaaa catgttgat gttttgggt cactgataa 
        ctttctcat gcataaaag tcacagcaa aaggcaagc 
        gagaaggac gagcgctga aaactcact tttggcgcg 
        caccaaagt caagtatcg aacaattaa gaaaaaaat 
        tagaagacg cgactctga aagcgatat tctgctttc 
        aggagatga tgaattgtt ggatcctga gtcagtacc 
        tataaatag acacgtgtt aacttaata tgtttatta 
        taagctggt taaaataat taagagact gtccaaata 
        tcgtttgca aaatcaagt gccttagtg tggttactt 
        gtgccaaat tattttaat cagcgcgtt agaaaacgg 
        ctgtaattt ctctctcat taagctcta atgatttaa 
        gctaagtta aatctggtt accgtgaga ttagtgata 
        tgtaggtta ctttaatag gtgctattt atgaacaaa 
        atagtcaaa agactctgt ttactagag tagtcatga 
        tcatgtact gttaaaatc tgggattga acacctcac 
        agaaccgtc ccgctttta tttatttgt gttaattat 
        tcactgaca ttccatatt ctcattgaa caatttatc 
        tcttgtttt aatcacgaa cgtcatatg tttaccata 
        tttatacca aatatttag atattaagc gtttttgtg 
        tacagaacg gataataaa tcgatttat tttcg
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY