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Gene information
General information
TC008821 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10411809..10413415
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008821
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01421

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    head appendages random not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head appendages size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    head irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax segment sometimes not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008821 RA
      MTETESKDC PTETIESLT KEAEALKKK LEDERQKLN 
      DVTLATVAE RLDIINYMN IKPRRTLKG HQAKVLCSD 
      WSPDKRHIV SSSQDGKMI IWDAFTTNK EHAVTMPTT 
      WVMACAYAP SGNLVACGG LDNKVTVYP LSLDEDVTL 
      KKKTVGTHT SYMSCCIFP NSDQQILTG SGDSTCALW 
      DVESGQLLQ SFHGHSADV MSIDLAPSE TGNTFVSGS 
      CDKMVLIWD MRSGQCVQS FEGHESDVN SVKFHPSGD 
      AVATGSDDA TCRLFDLRA DKEVAVYSK ESIIFGVNS 
      VDFSVSGRL LFAGYNDYT VNVWDTLKC VRVCLLYGH 
      ENRVSCLQV SPDGTALST GSWDYTLRV WA
    CDS sequence
    • >TC008821 RA
      atgaccgaa acggagtcg aaagactgt cccactgaa 
      accatcgag tccctcacc aaagaggcc gaagccttg 
      aaaaagaaa ctggaagat gagagacaa aaactcaac 
      gacgttaca ctggctacg gtggccgag cgcttggac 
      atcatcaac tacatgaac atcaaaccc cgacgaacc 
      ctaaagggc caccaagcc aaagtcctg tgttccgat 
      tggtccccg gacaagcgc cacattgtc tcatcatca 
      caagatggt aaaatgatc atttgggac gctttcact 
      actaacaaa gagcatgca gtcaccatg cctacaact 
      tgggtaatg gcttgtgct tacgcccca tcaggaaac 
      ctagtcgct tgcgggggg ttggataac aaagtcact 
      gtttatcct ctaagtctg gacgaggat gtgaccctc 
      aaaaaaaaa acagtcggg acgcacact agttacatg 
      agttgttgc atttttccc aactctgac cagcaaatt 
      ttgacgggg agtggcgac tcaacttgc gctttgtgg 
      gacgtagag tcgggccag ctactgcag agcttccac 
      ggacattcc gctgatgtt atgtcgata gatttggcc 
      ccgtctgag accggcaac actttcgtc tccgggagt 
      tgcgacaaa atggtattg atctgggac atgcgttcg 
      ggacaatgc gtccaatct tttgaagga cacgaatcg 
      gacgttaac agcgttaaa ttccatcca agtggcgac 
      gctgtagct acaggaagt gacgatgct acgtgtcgg 
      ttatttgat ctaagggct gacaaagag gttgcagtt 
      tacagcaaa gaaagtatt atttttggg gtgaattcg 
      gtcgatttc tccgtttca ggtcgactc ttattcgct 
      gggtataac gattatact gtcaatgtt tgggatact 
      cttaaatgt gtaagagtg tgtctgttg tacgggcac 
      gaaaatcga gtctcgtgt ttgcaagtc tcgccagat 
      ggcactgct ctttctaca gggtcgtgg gactacacc 
      cttagggtc tgggcttaa
    mRNA sequence
    • >TC008821 RA
      agtgggagt gttgtgggg ctaaaaatg accgaaacg 
      gagtcgaaa gactgtccc actgaaacc atcgagtcc 
      ctcaccaaa gaggccgaa gccttgaaa aagaaactg 
      gaagatgag agacaaaaa ctcaacgac gttacactg 
      gctacggtg gccgagcgc ttggacatc atcaactac 
      atgaacatc aaaccccga cgaacccta aagggccac 
      caagccaaa gtcctgtgt tccgattgg tccccggac 
      aagcgccac attgtctca tcatcacaa gatggtaaa 
      atgatcatt tgggacgct ttcactact aacaaagag 
      catgcagtc accatgcct acaacttgg gtaatggct 
      tgtgcttac gccccatca ggaaaccta gtcgcttgc 
      ggggggttg gataacaaa gtcactgtt tatcctcta 
      agtctggac gaggatgtg accctcaaa aaaaaaaca 
      gtcgggacg cacactagt tacatgagt tgttgcatt 
      tttcccaac tctgaccag caaattttg acggggagt 
      ggcgactca acttgcgct ttgtgggac gtagagtcg 
      ggccagcta ctgcagagc ttccacgga cattccgct 
      gatgttatg tcgatagat ttggccccg tctgagacc 
      ggcaacact ttcgtctcc gggagttgc gacaaaatg 
      gtattgatc tgggacatg cgttcggga caatgcgtc 
      caatctttt gaaggacac gaatcggac gttaacagc 
      gttaaattc catccaagt ggcgacgct gtagctaca 
      ggaagtgac gatgctacg tgtcggtta tttgatcta 
      agggctgac aaagaggtt gcagtttac agcaaagaa 
      agtattatt tttggggtg aattcggtc gatttctcc 
      gtttcaggt cgactctta ttcgctggg tataacgat 
      tatactgtc aatgtttgg gatactctt aaatgtgta 
      agagtgtgt ctgttgtac gggcacgaa aatcgagtc 
      tcgtgtttg caagtctcg ccagatggc actgctctt 
      tctacaggg tcgtgggac tacaccctt agggtctgg 
      gcttaaaac ggaattatt gcatttcaa tggcattta 
      agaatattt gttctgtgt ttatagact tatcagttc 
      agcagagca gaggtgttt gtatcgagc tgactgtag 
      cgaaatgtc tgtatttat ttaagtgca atctaaaat 
      attgttgac tgtttctga agattagtt gatgtaata 
      ataaaactt ccagttctg t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY