Status
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Gene information
General information
TC008808 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10135778..10139938
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008808
    iBeetle screen iB_01420

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008808 RA
      MQEPRDHLK NRVYYAAKD GMSLALYAL LVGANKQEV 
      EAILDEKFV EDDGQECTP LIVAARQGH DKVMRMMLT 
      KFKPDLEKE GTVKFDGYV IEGASALWC AACAGHLNV 
      VKTLVKAGA DVNHPTKSN STPLRAACF DGRLDIVKY 
      LTYHRADIN IANKYNNTC LMIAAYKGH LDVVSFLLE 
      HGANANEKA LCGATALHF AAECGHLPV VKELLKYNA 
      VFSTNDSGM TPIKAAAER TRADVVEYL VERPEISKE 
      EQIEALELL GASYANDKE NYDIVMAYK YLHRSMMLR 
      YSDPDSPVK KKLITPIPA YEHWVESQT VYELEAIQN 
      NHNSIHMEA LTIRERILG SQNPEVPHP VIYRGAVFA 
      DNARFERCL ELWLHALRL RQKNHISVV KDLLRFAQV 
      FSQMLHVNV KVTYDHVVQ VLAAAITEL ERNKYKLSK 
      PGPKDDPET VMEELESNL TTTLYLLTI LTKLLKECS 
      EEERFNVNR MVFTLNQLN VTLRDGQTL LHLACNAET 
      PVDDFHTND VCKFPCAET TRLLIKCGA DVNAMDYDR 
      NTPLHVIVN YHKPISDFL TLHSIITDL TEAGAHLDC 
      VNKRGETPL EASATGSVA EIILKTQMK LSLKCIAAN 
      AVKHHKLTY QGQVPEALE SFIELHGPG IVKKA
    CDS sequence
    • >TC008808 RA
      atgcaagaa cctcgcgac cacttaaaa aatcgtgtt 
      tattatgcc gccaaggac ggcatgtcc ctagccctg 
      tacgctctg ctggtgggt gctaacaaa caagaagtg 
      gaagccatc ctcgacgag aagttcgtc gaggatgac 
      ggccaggaa tgcacccct ttgatcgtg gcggcgcgg 
      cagggtcac gacaaggtg atgcgcatg atgttgacc 
      aagtttaag cccgatctg gagaaagaa ggcaccgtc 
      aagttcgat ggatatgtg atcgagggg gccagtgct 
      ctgtggtgt gccgcgtgt gcaggtcac ttaaacgtg 
      gtaaaaaca ctggtgaag gccggggct gacgtaaac 
      caccccaca aaatccaat tctacgcct ttgcgggcc 
      gcctgtttc gacggtcgc ttggacatc gtcaagtac 
      ctgacttac caccgggct gatattaac atagcaaac 
      aaatacaat aacacctgt cttatgata gccgcgtat 
      aaaggccat ttggacgtc gtcagtttt ctcctagag 
      catggtgcc aacgcgaac gagaaagcc ctttgtggg 
      gctacggcc ctgcatttc gccgccgag tgcggacac 
      cttcccgtc gttaaagaa ctgttgaaa tacaacgct 
      gttttttca acgaacgac tcgggaatg actcccatt 
      aaagcggca gcggaaaga acccgagcc gatgtggtg 
      gaatatctc gtcgaaaga ccggaaatt agtaaagag 
      gagcagatc gaagcactg gaattgctg ggagcgtcg 
      tacgccaac gataaggag aattacgac atcgtcatg 
      gcttacaag tacttgcac aggtcaatg atgctgagg 
      tacagcgat ccggacagt ccggtcaag aaaaaactg 
      ataactcca attcccgcg tacgaacac tgggtcgaa 
      tctcagacg gtttacgaa ctcgaagcc atccaaaac 
      aaccacaat tccatccat atggaggct ttgaccatt 
      cgggaacga atcttgggg tcgcagaac ccagaagtg 
      cctcatcct gtgatatac aggggcgct gttttcgca 
      gataatgcg cgatttgag cggtgttta gagctgtgg 
      ttgcacgcg ttgaggttg aggcagaaa aaccatatt 
      tcggttgtt aaagacttg ctcaggttt gctcaagtg 
      ttttcgcag atgttacac gtgaatgtc aaggttacg 
      tacgatcac gtggtgcag gttttagcg gcggctatt 
      acggagttg gagaggaat aagtacaag ttgagcaag 
      ccggggccc aaggatgat cctgagact gtgatggag 
      gagcttgaa agtaatctc actacgact ctttatctc 
      ctgacaata ttaacaaaa ttactcaag gagtgttca 
      gaggaggaa cgcttcaat gtaaatcgg atggttttt 
      actcttaac cagcttaat gtaacgctg agagacggt 
      cagacgctt ctgcacctg gcgtgtaat gcggagaca 
      cctgtggac gatttccat actaacgat gtgtgcaaa 
      tttccctgt gctgagacc acccgactg ttgataaaa 
      tgtggcgct gatgtgaat gccatggat tatgaccgt 
      aatactccc cttcatgtg attgttaat taccataag 
      ccaatttcg gattttttg actttgcat tcgatcata 
      accgacttg accgaagcg ggggcgcat ttagattgt 
      gtaaataag agaggggaa acaccgctg gaagcctcg 
      gctacaggt agtgtggcc gagattatt ttaaagact 
      caaatgaaa cttagtctg aagtgtatc gctgccaat 
      gctgttaaa catcataaa ctcacttat caagggcaa 
      gttcctgaa gctctcgaa tcttttata gagttacac 
      ggtccgggg atcgtgaag aaggcctag
    mRNA sequence
    • >TC008808 RA
      aaggtgatg aaaggttaa gtttgaagt ttcttgatt 
      ttgaggttg atttacggt ttgcaggtt ccaggagtt 
      taaatttag aagcgatat gtaattgtg ccatattct 
      gatcaaaac agattttta aattacgag aaaatcacg 
      tgacaaaat ttgcaaatg acagatctg tcaatgctt 
      gcccccatc ctacgtcaa acagcaagc aaatgcttt 
      cgctccctt gagcaaatc agtgaatcg cagtccgcg 
      ctcgagtta gttcaaaat agcatttcg cgaagtatg 
      tgttgaaac agtgaacga tgcaagaac ctcgcgacc 
      acttaaaaa atcgtgttt attatgccg ccaaggacg 
      gcatgtccc tagccctgt acgctctgc tggtgggtg 
      ctaacaaac aagaagtgg aagccatcc tcgacgaga 
      agttcgtcg aggatgacg gccaggaat gcacccctt 
      tgatcgtgg cggcgcggc agggtcacg acaaggtga 
      tgcgcatga tgttgacca agtttaagc ccgatctgg 
      agaaagaag gcaccgtca agttcgatg gatatgtga 
      tcgaggggg ccagtgctc tgtggtgtg ccgcgtgtg 
      caggtcact taaacgtgg taaaaacac tggtgaagg 
      ccggggctg acgtaaacc accccacaa aatccaatt 
      ctacgcctt tgcgggccg cctgtttcg acggtcgct 
      tggacatcg tcaagtacc tgacttacc accgggctg 
      atattaaca tagcaaaca aatacaata acacctgtc 
      ttatgatag ccgcgtata aaggccatt tggacgtcg 
      tcagttttc tcctagagc atggtgcca acgcgaacg 
      agaaagccc tttgtgggg ctacggccc tgcatttcg 
      ccgccgagt gcggacacc ttcccgtcg ttaaagaac 
      tgttgaaat acaacgctg ttttttcaa cgaacgact 
      cgggaatga ctcccatta aagcggcag cggaaagaa 
      cccgagccg atgtggtgg aatatctcg tcgaaagac 
      cggaaatta gtaaagagg agcagatcg aagcactgg 
      aattgctgg gagcgtcgt acgccaacg ataaggaga 
      attacgaca tcgtcatgg cttacaagt acttgcaca 
      ggtcaatga tgctgaggt acagcgatc cggacagtc 
      cggtcaaga aaaaactga taactccaa ttcccgcgt 
      acgaacact gggtcgaat ctcagacgg tttacgaac 
      tcgaagcca tccaaaaca accacaatt ccatccata 
      tggaggctt tgaccattc gggaacgaa tcttggggt 
      cgcagaacc cagaagtgc ctcatcctg tgatataca 
      ggggcgctg ttttcgcag ataatgcgc gatttgagc 
      ggtgtttag agctgtggt tgcacgcgt tgaggttga 
      ggcagaaaa accatattt cggttgtta aagacttgc 
      tcaggtttg ctcaagtgt tttcgcaga tgttacacg 
      tgaatgtca aggttacgt acgatcacg tggtgcagg 
      ttttagcgg cggctatta cggagttgg agaggaata 
      agtacaagt tgagcaagc cggggccca aggatgatc 
      ctgagactg tgatggagg agcttgaaa gtaatctca 
      ctacgactc tttatctcc tgacaatat taacaaaat 
      tactcaagg agtgttcag aggaggaac gcttcaatg 
      taaatcgga tggttttta ctcttaacc agcttaatg 
      taacgctga gagacggtc agacgcttc tgcacctgg 
      cgtgtaatg cggagacac ctgtggacg atttccata 
      ctaacgatg tgtgcaaat ttccctgtg ctgagacca 
      cccgactgt tgataaaat gtggcgctg atgtgaatg 
      ccatggatt atgaccgta atactcccc ttcatgtga 
      ttgttaatt accataagc caatttcgg attttttga 
      ctttgcatt cgatcataa ccgacttga ccgaagcgg 
      gggcgcatt tagattgtg taaataaga gaggggaaa 
      caccgctgg aagcctcgg ctacaggta gtgtggccg 
      agattattt taaagactc aaatgaaac ttagtctga 
      agtgtatcg ctgccaatg ctgttaaac atcataaac 
      tcacttatc aagggcaag ttcctgaag ctctcgaat 
      cttttatag agttacacg gtccgggga tcgtgaaga 
      aggcctagt caatcgttt tgttagatt ttttattgt 
      aaatagcgt gcttttcca atttgttct tttgctgat 
      tgttttatt agctttaag ttgtagagt tgttaagca 
      aacgaacaa attgttttt gtcatttac caataattg 
      gtgttattt tttgagact gctaattag gatttttta 
      tcagatttt taattgtac ttgttattt gaggcgtta 
      ttagtatgt ctccttttt tgttatttt tgtacaaaa 
      ttgtgccgt tccttcaat ttgtgtcta acctcgtgg 
      cttttctat gtttaggtt acaaaaggc aaagactgc 
      tacaactgg acactgata ataacatta acatttcaa 
      accaaaatt atgaaattt tggcgagtg aagcaacaa 
      ataattgct tgattgagc ttttggaaa caagtgttt 
      ttaaagaaa attttaaca tttaagcga aaatttggc 
      ggttatagt attatttat ttataaagc cgattgaaa 
      atagcaata aggacgttt agaaaattt atagaaacg 
      gcacgagga ttagatttg tttgcaacc taacctttt 
      ctgtaatgt ctaagtgac ttccttatg atttttagg 
      tgtaatttg taaattatc aaatcaaga tggtgcata 
      atagagata gggatgaga caagatatt tatatttta 
      cttatgtat tttttctat tctgaaata aacccaagt 
      gtgatat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY