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Gene information
General information
TC008785 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9863263..9865715
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008785
    iBeetle screen iB_01415

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature potentially pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008785 RA
      MADPVEPPP LFENVDILS DRKEDEDLF VSARQEQAE 
      PYKDEPQEG LNGDADLTN LSLDDQKPE LETVPINNS 
      GTLPDTMEQ VEAAGGDQF LEISITEPQ KVGDGMSAY 
      MAYRVTTKT NMPIFKKRE FSVTRRFSD FLGLHDKLS 
      EKYLKVGRI IPPAPEKSV IGMTKIKIS SQAEGSPSN 
      GNDFVERRR ASLERYLKR TAQHPVLVL DPDFREFLE 
      SDIELPKAT STSALSSAG VMRLFNKVG ETVNKITYK 
      MDETDPWFE DKLAHIEAL ETQLRKLHT NVEAMVAYR 
      KELSHLTNG VSRSAAMLS ACEDHNSLS MALSNLADT 
      EEKVEALHL EQANTDFFI LCELLKDYL GLLGAVRDA 
      FHERTKLFQ HWQHSQQML AKKREAKAK MELTNRTDK 
      VDQAGAEVI EWEAKVERG QENFDKISK MIKTEVERF 
      EKCRIHDFK IMFIKYFEN HLNHQAQLV KYWEAFLPQ 
      AKAIA
    CDS sequence
    • >TC008785 RA
      atggcagac cctgtggag ccgccccct ttatttgaa 
      aacgtggac atcctttcc gaccgaaaa gaagacgaa 
      gatctcttc gtgtcagcc agacaggag caggccgaa 
      ccgtacaaa gatgagccc caggaaggc ctcaacggc 
      gacgccgac ctcaccaac ctctccctg gacgaccag 
      aaacccgaa ctggagact gtcccaatc aacaatagt 
      ggaacattg ccagacacc atggaacaa gtcgaggct 
      gcaggcggc gatcaattt ttggaaata tccatcacg 
      gagccccaa aaagtgggt gacggcatg agcgcttat 
      atggcttac cgggtcaca acaaaaacc aacatgccg 
      atatttaaa aaacgcgaa ttttcggtg acgaggcgt 
      ttcagcgac tttctgggc ctccacgac aaattatca 
      gagaagtac ctcaaggtc gggaggatc ataccgcca 
      gcgcctgaa aagagtgtc attggcatg accaaaatc 
      aagatctcc agtcaagcg gagggcagt ccttcgaac 
      ggaaacgac tttgtcgaa cgcaggcgc gcgtccttg 
      gagcgttac ctaaaacgc actgcccaa caccctgtc 
      ctggtactg gaccccgac tttagggag tttctggag 
      tcagatatt gagctgccg aaggccaca agcacgtct 
      gccttgagt agcgccggt gttatgagg ctcttcaat 
      aaggttggc gagactgtg aacaaaatc acctacaag 
      atggatgag actgatcca tggtttgag gataagttg 
      gctcacatt gaggctctg gagactcag ctgaggaaa 
      ttgcatacc aatgttgag gcgatggtg gcttacaga 
      aaggaactg agtcacttg actaacggc gtttcacgg 
      tctgcggcg atgttgagc gcttgtgag gaccacaac 
      tcgctctca atggcgctg tccaatttg gccgatact 
      gaggaaaaa gttgaagca ttgcatttg gaacaggcc 
      aacacggat ttcttcatt ctctgtgag ttactgaag 
      gattattta gggcttctc ggggctgtt cgtgacgcc 
      tttcatgaa agaacaaag cttttccaa cattggcaa 
      cactcgcaa caaatgttg gccaagaaa agggaagcc 
      aaggctaag atggagctc acaaacagg acagacaag 
      gtcgaccaa gctggcgct gaagtgata gagtgggaa 
      gccaaagtc gaacgtggc caagagaat tttgataag 
      atctcgaaa atgatcaaa acggaagtg gagaggttt 
      gaaaagtgt cgcattcac gactttaaa attatgttt 
      ataaagtat tttgaaaac cacctcaat caccaagcc 
      cagctcgtt aagtactgg gaagccttc ttgccgcaa 
      gccaaagcg attgcttga
    mRNA sequence
    • >TC008785 RA
      cgtgaacgt tttatttgt tttacgcga gatttgttt 
      acgaaaata aaagtaaag tttgcttta caaaaccct 
      cgcctgagg ttccatcct tagtcatca ttaagtaaa 
      tatttccaa gcactttaa agcgaaaaa tcgtcgtta 
      gcacctggt gaataatta ggttggcat tccctattg 
      ccattaatt tttttgatt gacgtttgt caatttaac 
      agaccggcc gcgtcaaat aattaattt tatcgcctt 
      ttgaaataa aatggcaga ccctgtgga gccgccccc 
      tttatttga aaacgtgga catcctttc cgaccgaaa 
      agaagacga agatctctt cgtgtcagc cagacagga 
      gcaggccga accgtacaa agatgagcc ccaggaagg 
      cctcaacgg cgacgccga cctcaccaa cctctccct 
      ggacgacca gaaacccga actggagac tgtcccaat 
      caacaatag tggaacatt gccagacac catggaaca 
      agtcgaggc tgcaggcgg cgatcaatt tttggaaat 
      atccatcac ggagcccca aaaagtggg tgacggcat 
      gagcgctta tatggctta ccgggtcac aacaaaaac 
      caacatgcc gatatttaa aaaacgcga attttcggt 
      gacgaggcg tttcagcga ctttctggg cctccacga 
      caaattatc agagaagta cctcaaggt cgggaggat 
      cataccgcc agcgcctga aaagagtgt cattggcat 
      gaccaaaat caagatctc cagtcaagc ggagggcag 
      tccttcgaa cggaaacga ctttgtcga acgcaggcg 
      cgcgtcctt ggagcgtta cctaaaacg cactgccca 
      acaccctgt cctggtact ggaccccga ctttaggga 
      gtttctgga gtcagatat tgagctgcc gaaggccac 
      aagcacgtc tgccttgag tagcgccgg tgttatgag 
      gctcttcaa taaggttgg cgagactgt gaacaaaat 
      cacctacaa gatggatga gactgatcc atggtttga 
      ggataagtt ggctcacat tgaggctct ggagactca 
      gctgaggaa attgcatac caatgttga ggcgatggt 
      ggcttacag aaaggaact gagtcactt gactaacgg 
      cgtttcacg gtctgcggc gatgttgag cgcttgtga 
      ggaccacaa ctcgctctc aatggcgct gtccaattt 
      ggccgatac tgaggaaaa agttgaagc attgcattt 
      ggaacaggc caacacgga tttcttcat tctctgtga 
      gttactgaa ggattattt agggcttct cggggctgt 
      tcgtgacgc ctttcatga aagaacaaa gcttttcca 
      acattggca acactcgca acaaatgtt ggccaagaa 
      aagggaagc caaggctaa gatggagct cacaaacag 
      gacagacaa ggtcgacca agctggcgc tgaagtgat 
      agagtggga agccaaagt cgaacgtgg ccaagagaa 
      ttttgataa gatctcgaa aatgatcaa aacggaagt 
      ggagaggtt tgaaaagtg tcgcattca cgactttaa 
      aattatgtt tataaagta ttttgaaaa ccacctcaa 
      tcaccaagc ccagctcgt taagtactg ggaagcctt 
      cttgccgca agccaaagc gattgcttg actagagga 
      tatctgcaa agaatagca gaacagaca ttcctattg 
      gagatttcc agaagttta atgtgtaat atacaattt 
      ttgaactta tttagcacg aggagtctc caagttcgt 
      ccctttttt atttctgtc atttacaca taatcaaat 
      atttgtagt ttataaccc tattgttga atctattta 
      tgttacttt gtagccctt gtgtattta cagcgctac 
      gttaaaata tagctcctt tgttatgta aaaatcgcc 
      taaagaatt atatttgtt atgtgcttt gtgtgaaac 
      atgaatatt ataccctac attgtgata agaatatgc 
      tcagttttt gaagcttga ttcttgttc aagtctagt 
      gcaagttaa ggtaattta ggtccctat tggcattgt 
      gtacaagtg atgcatgaa ttatcgaat attgtactg 
      cttccatta tgtaagaaa aatttaaat aaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY