Status
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Gene information
General information
TC008781 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9836183..9838087
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008781
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01414

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008781 RA
      MLAKLILLQ LMSAFALGQ DCLSRDYGN GGTVCVCNS 
      NHCDTVPKP KKLEPSELL IYTSNKAGL RFNLEKTNF 
      KPCAFSDRI VIDPKTKYQ TILGWGGAF TDAAGINIA 
      SLEESLQTK LLESYFSEN GLEYSLCRV PIGGTDFSV 
      RAYSYDDGK EDKDLTNFK LAEEDHKYK IPYIKKALE 
      LTENRLKLF ASAWTAPKW MKTDGQYAG LGGSLKKEM 
      YQTWVDYFI KFFDSYKEQ GISFWGVTT GNEPSLAIS 
      PTNRINNVG WGPKNMGTW VRDNLGPTI RNSAYSDMK 
      IMILDDQRS LLPWYADEV LKDNTVRKY VDGVAVHWY 
      HNIWPLFWP ASVLTFTHW HFPEKFILA TEACNGVGE 
      ESVVLGSWE RGEKYSYDI IKDLQNWVT GWIDWNMVL 
      DLSGGPTYI SNNVDAPII VNASAGEFY KQPMYYHLG 
      HFSKFVPPN SVLIKTSFA NKDLLTVGF QRPDNATVL 
      VILNKTGKT IPVNVVDPN KGVAQVEIK ARSIVTVFY 
      YSQNYK
    CDS sequence
    • >TC008781 RA
      atgcttgca aaacttatc cttctccag ctaatgtca 
      gcgtttgcc ctaggacaa gactgttta agcagagat 
      tacggcaat ggaggcact gtatgtgtt tgcaattcg 
      aatcattgt gacactgtc ccaaagccg aaaaagtta 
      gaaccgagt gagcttttg atttacaca tccaacaaa 
      gctggtctg agatttaat ctcgaaaaa acaaacttc 
      aaaccttgt gctttctcg gatcggata gtcattgat 
      cctaaaacc aagtaccaa acgatattg gggtggggc 
      ggcgccttt acagacgca gcaggaatc aacattgca 
      tcgctggag gaaagtctt caaaccaaa cttctagaa 
      tcttatttt tctgaaaac ggtcttgag tatagcttg 
      tgtagagta ccgatcggc ggtaccgat ttttcagta 
      cgtgcttac tcgtacgac gacggtaaa gaagacaaa 
      gatttgacc aactttaaa ttagccgaa gaagaccac 
      aaatacaaa attccgtac attaaaaaa gctctagag 
      ttgactgaa aataggtta aaattgttt gcgtcggct 
      tggactgca cccaaatgg atgaaaact gacggacag 
      tacgccggt cttggcggt tctcttaag aaagaaatg 
      tatcaaaca tgggtcgac tatttcatc aaatttttt 
      gatagttat aaagagcaa ggaatcagt ttttggggc 
      gtaactaca ggaaatgag cccagttta gctatatcg 
      cctactaac cgcataaat aacgtcggg tggggccca 
      aaaaacatg ggaacatgg gttcgcgat aatttagga 
      ccaacaatt cgaaactcg gcatattca gacatgaag 
      attatgata ttggatgat caaagatcg cttcttccg 
      tggtatgcc gatgaggtt cttaaagat aatactgtt 
      cgtaaatac gtagatgga gtcgctgtg cattggtat 
      cacaatatt tggcctctg ttttggcct gcttcagta 
      ctgactttc actcattgg catttccct gaaaagttt 
      attttagcc actgaggct tgcaatggt gtaggtgag 
      gagtcggtg gttcttggg tcgtgggaa cgaggcgaa 
      aaatattcg tatgatata attaaggat ttgcaaaac 
      tgggtgacc ggttggatc gactggaac atggtgctc 
      gatttatca ggtggaccc acctacatt agcaataac 
      gttgatgcg cccataatt gttaacgcg tctgcgggt 
      gaattttac aaacaaccc atgtactac cacttaggc 
      catttctca aaatttgtg ccaccgaat tcggtgctg 
      attaagacg agtttcgcc aacaaagac ttacttacg 
      gtaggtttc caaagacct gataatgct acagtcttg 
      gtaattcta aacaaaact ggaaaaact atacctgta 
      aatgttgtc gatcctaat aaaggtgtc gcacaggtg 
      gaaatcaaa gcaagatca attgtcaca gttttctat 
      tatagccaa aattataaa taa
    mRNA sequence
    • >TC008781 RA
      cttagatct ggcacaaga tgcttgcaa aacttatcc 
      ttctccagc taatgtcag cgtttgccc taggacaag 
      actgtttaa gcagagatt acggcaatg gaggcactg 
      tatgtgttt gcaattcga atcattgtg acactgtcc 
      caaagccga aaaagttag aaccgagtg agcttttga 
      tttacacat ccaacaaag ctggtctga gatttaatc 
      tcgaaaaaa caaacttca aaccttgtg ctttctcgg 
      atcggatag tcattgatc ctaaaacca agtaccaaa 
      cgatattgg ggtggggcg gcgccttta cagacgcag 
      caggaatca acattgcat cgctggagg aaagtcttc 
      aaaccaaac ttctagaat cttattttt ctgaaaacg 
      gtcttgagt atagcttgt gtagagtac cgatcggcg 
      gtaccgatt tttcagtac gtgcttact cgtacgacg 
      acggtaaag aagacaaag atttgacca actttaaat 
      tagccgaag aagaccaca aatacaaaa ttccgtaca 
      ttaaaaaag ctctagagt tgactgaaa ataggttaa 
      aattgtttg cgtcggctt ggactgcac ccaaatgga 
      tgaaaactg acggacagt acgccggtc ttggcggtt 
      ctcttaaga aagaaatgt atcaaacat gggtcgact 
      atttcatca aattttttg atagttata aagagcaag 
      gaatcagtt tttggggcg taactacag gaaatgagc 
      ccagtttag ctatatcgc ctactaacc gcataaata 
      acgtcgggt ggggcccaa aaaacatgg gaacatggg 
      ttcgcgata atttaggac caacaattc gaaactcgg 
      catattcag acatgaaga ttatgatat tggatgatc 
      aaagatcgc ttcttccgt ggtatgccg atgaggttc 
      ttaaagata atactgttc gtaaatacg tagatggag 
      tcgctgtgc attggtatc acaatattt ggcctctgt 
      tttggcctg cttcagtac tgactttca ctcattggc 
      atttccctg aaaagttta ttttagcca ctgaggctt 
      gcaatggtg taggtgagg agtcggtgg ttcttgggt 
      cgtgggaac gaggcgaaa aatattcgt atgatataa 
      ttaaggatt tgcaaaact gggtgaccg gttggatcg 
      actggaaca tggtgctcg atttatcag gtggaccca 
      cctacatta gcaataacg ttgatgcgc ccataattg 
      ttaacgcgt ctgcgggtg aattttaca aacaaccca 
      tgtactacc acttaggcc atttctcaa aatttgtgc 
      caccgaatt cggtgctga ttaagacga gtttcgcca 
      acaaagact tacttacgg taggtttcc aaagacctg 
      ataatgcta cagtcttgg taattctaa acaaaactg 
      gaaaaacta tacctgtaa atgttgtcg atcctaata 
      aaggtgtcg cacaggtgg aaatcaaag caagatcaa 
      ttgtcacag ttttctatt atagccaaa attataaat 
      aaattatta atttaccca ctttatgcc ttcacaatt 
      catgcgtat tataattaa ttaatcatt catcatcct 
      ttacaaaaa tgtaaataa attatttaa acaatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY