TC008777
Overview
Gene identifier: TC008777
Genomic location:
NC_007422.5:9817333..9819237
View mRNA sequences
View coding sequences
View protein sequences
View gene expression at BeetleAtlas
View orthologs at OrthoDB
Closest fly homologs
No fly homologs
Gene ontology
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Tribolium
gene by filling in the fields. Repeat to add several GO terms. Search
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Tribolium
data should be entered - do not define terms just based on
Drosophila
knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
*GO ID:
GO:
0 / 7
*Gene product
protein
*Evidence code:
*PubMed ID:
PMID:
Quotation:
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Publications
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*DOI:
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RNAi phenotypes contributed by the community
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*Gene:
*dsRNA:
iB_01413
New sequence
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*Sequence of the new dsRNA:
*Concentration:
ng/μl
*Injected stage:
*Injected strain:
*Reference:
DOI
*Phenotype description:
*Affected structure:
Affected process:
*Penetrance:
Number of animals analysed:
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RNAi phenotypes from iBeetle screen iB_01413
Workflow Pupal Screen
Workflow Larval Screen
View left primer
View dsRNA sequence
View right primer
Phenotype after pupal injection
Analysis of embryonic musculature and early development
larval musculature random not present
50%
day 14
embryo/egg with embryonic tissue, no cuticle, no fluorescence
30%
day 14
larval musculature random pattern irregular
50%
day 14
embryo/egg not developed/not fertilized
30%
day 14
affected eggs/embryos/larvae
100%
day 14
Analysis of larval stage 1 cuticle
head & thorax & abdomen segment random not present
30%
day 15
cuticle remnants with unidentifyable segments
30%
day 15
embryo/egg without visible larval cuticle in cuticle preparation
80%
day 15
affected eggs/embryos/larvae
100%
day 15
larva number hatched none (< 10 %)
day 13