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Gene information
General information
TC008777 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9817333..9819237
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008777
    iBeetle screen iB_01413

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature random pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008777 RA
      MFKLFFLLL IISFCSAFD FIFQPGPLV PASKGEIWP 
      KPQHENKLD DGFFSLLPT FFHFNPIGN ICNTLTEAL 
      DRYRKLIIF NNRRIKEVY YKARSCYEG GDQNFLGYL 
      TSVEVELTG ACNDEEYPS FEMKEEYVV NVTSTVQRI 
      SSDTIWGIL RGLETFSQL IYLTDDYSC HRIGTTSIH 
      DYPRFAHRG LLLDTSRHY IPKEHILKL IETMSYNKL 
      NVFHWHITD DYSFPYVSK AFPQMSNKG AFHPTLMIY 
      EQDFVSEVQ EYARKRGIR VLAEFDTPG HTLSWGLGN 
      PDLLTDCHN VPQLKWGPI NPIKNTTYD FIFKLFEEI 
      KSVFKDEYT HLGGDEVDF SCWKSNPEI NQWMAEHQM 
      EGDYVALQS HYIQKLINH VDSLGLNSI VWEEVFTNG 
      VQLPKSTVV NVWISDDPK TTLKQVTEA GHPTIISSY 
      WYLDILKTG GDWLKFYNA DPQDFDGTD EQKRLVLGG 
      EACMWSEVV DEYNLEPRV WPRASVAAE RFWSPPDTP 
      KSAQNLGEL WTIASRLQE QTCRMNRRG VAAQPPSGP 
      SVCF
    CDS sequence
    • >TC008777 RA
      atgttcaaa ctatttttc cttttactc ataatttca 
      ttttgttcg gctttcgat ttcattttc caacccggc 
      ccacttgtt ccggcttcg aaaggcgaa atatggcca 
      aagccccaa catgaaaat aaattagac gatggattc 
      ttctctttg ttaccaaca ttctttcat ttcaatcca 
      atcggaaac atctgtaac acgttaact gaagctttg 
      gacagatac agaaagttg atcatattt aacaatcgc 
      cgaataaag gaggtttat tacaaagcg cgcagttgc 
      tacgaaggc ggagatcaa aattttttg ggctatttg 
      acaagcgtg gaagttgaa ttgactggt gcttgtaac 
      gatgaagag tacccctca tttgagatg aaagaagaa 
      tatgttgta aacgtaacg tcaacagtg caaagaatt 
      tcctcagac actatatgg ggaatactt cgggggctc 
      gaaactttc tcacaattg atatacttg acagacgat 
      tacagttgt cacagaatt ggaacaact tcaattcac 
      gattacccc agatttgcc catcgcgga ctacttttg 
      gacacttcc cgtcattac atcccaaaa gagcacatt 
      ttgaagtta atcgagaca atgtcttac aacaaatta 
      aatgttttc cactggcat atcactgac gattacagt 
      tttccctac gtcagtaaa gctttcccg caaatgagc 
      aataaggga gcattccat ccgactctc atgatttac 
      gagcaagac tttgtaagc gaagtgcaa gagtatgct 
      cgaaaacgc ggaattcgt gttttggca gagttcgat 
      acccctggc cataccctt tcttggggt ctgggaaac 
      ccagacttg ctcaccgat tgccacaac gtaccccaa 
      cttaagtgg ggacccatc aaccccatt aaaaacacc 
      acctacgac ttcattttc aaactattc gaagaaatc 
      aaaagcgtt ttcaaagac gaatacacg caccttggc 
      ggcgacgaa gtcgatttc tcttgctgg aaaagcaac 
      ccagaaatc aaccaatgg atggctgag caccagatg 
      gagggcgat tacgtcgcc ctgcaaagc cactacatc 
      caaaaactc atcaaccac gtcgattct ttaggttta 
      aactcaatt gtttgggaa gaagtgttc accaacggt 
      gtccaacta cccaaatcc acagtagtc aacgtctgg 
      attagcgat gacccgaaa acaaccctc aaacaagta 
      acagaagct ggccatcca acaatcatc tcatcttac 
      tggtatttg gatattttg aagaccgga ggtgattgg 
      ttaaaattc tacaatgct gacccgcaa gactttgat 
      ggtactgac gaacaaaaa agacttgtt cttggaggg 
      gaggcgtgt atgtggagc gaagtcgtt gatgagtat 
      aacttggag ccaagggtg tggccgaga gctagtgtg 
      gcggcggag aggttttgg tcgcctcca gacactccc 
      aaaagtgcg caaaattta ggagagctt tggactatc 
      gctagcagg ctacaagag cagacttgc agaatgaac 
      aggagaggg gtggcggca cagccgcct tcaggacca 
      agtgtttgt ttttaa
    mRNA sequence
    • >TC008777 RA
      acacgatca tgatgttca aactatttt tccttttac 
      tcataattt cattttgtt cggctttcg atttcattt 
      tccaacccg gcccacttg ttccggctt cgaaaggcg 
      aaatatggc caaagcccc aacatgaaa ataaattag 
      acgatggat tcttctctt tgttaccaa cattctttc 
      atttcaatc caatcggaa acatctgta acacgttaa 
      ctgaagctt tggacagat acagaaagt tgatcatat 
      ttaacaatc gccgaataa aggaggttt attacaaag 
      cgcgcagtt gctacgaag gcggagatc aaaattttt 
      tgggctatt tgacaagcg tggaagttg aattgactg 
      gtgcttgta acgatgaag agtacccct catttgaga 
      tgaaagaag aatatgttg taaacgtaa cgtcaacag 
      tgcaaagaa tttcctcag acactatat ggggaatac 
      ttcgggggc tcgaaactt tctcacaat tgatatact 
      tgacagacg attacagtt gtcacagaa ttggaacaa 
      cttcaattc acgattacc ccagatttg cccatcgcg 
      gactacttt tggacactt cccgtcatt acatcccaa 
      aagagcaca ttttgaagt taatcgaga caatgtctt 
      acaacaaat taaatgttt tccactggc atatcactg 
      acgattaca gttttccct acgtcagta aagctttcc 
      cgcaaatga gcaataagg gagcattcc atccgactc 
      tcatgattt acgagcaag actttgtaa gcgaagtgc 
      aagagtatg ctcgaaaac gcggaattc gtgttttgg 
      cagagttcg atacccctg gccataccc tttcttggg 
      gtctgggaa acccagact tgctcaccg attgccaca 
      acgtacccc aacttaagt ggggaccca tcaacccca 
      ttaaaaaca ccacctacg acttcattt tcaaactat 
      tcgaagaaa tcaaaagcg ttttcaaag acgaataca 
      cgcaccttg gcggcgacg aagtcgatt tctcttgct 
      ggaaaagca acccagaaa tcaaccaat ggatggctg 
      agcaccaga tggagggcg attacgtcg ccctgcaaa 
      gccactaca tccaaaaac tcatcaacc acgtcgatt 
      ctttaggtt taaactcaa ttgtttggg aagaagtgt 
      tcaccaacg gtgtccaac tacccaaat ccacagtag 
      tcaacgtct ggattagcg atgacccga aaacaaccc 
      tcaaacaag taacagaag ctggccatc caacaatca 
      tctcatctt actggtatt tggatattt tgaagaccg 
      gaggtgatt ggttaaaat tctacaatg ctgacccgc 
      aagactttg atggtactg acgaacaaa aaagacttg 
      ttcttggag gggaggcgt gtatgtgga gcgaagtcg 
      ttgatgagt ataacttgg agccaaggg tgtggccga 
      gagctagtg tggcggcgg agaggtttt ggtcgcctc 
      cagacactc ccaaaagtg cgcaaaatt taggagagc 
      tttggacta tcgctagca ggctacaag agcagactt 
      gcagaatga acaggagag gggtggcgg cacagccgc 
      cttcaggac caagtgttt gtttttaag ctaatgaat 
      tattgtttt ttaataatt ttgaataaa atattgtta 
      actca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY