Status
Please wait ...
Gene information
General information
TC008773 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9730073..9734788
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008773
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01412

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008773 RA
      MFKVGSIRK IEVKNFVTY SYAELYPGP NLNMLIGPN 
      GTGKSTIVA AIILGLGGN PKTVGRGVR VSEYVKHNC 
      EEATIHIYL QGRKDNDFI KITRIFNTH DKTGWLVNN 
      QRVTLKEVM DCIKQYNIQ VDNLCQFLP QDRVQDFAK 
      MNQQQLLKE TQVALCRTD LIEKQEALI ACKNNHKQL 
      TEAIDKNGA KLQETRDAN MRLEGKIEN FSRKKKFLS 
      QIEGIDRKI AWLQYDDLY EKMTETKAD LAKATKIYE 
      KHKSATKPA EKEIQKAKQ VVQELQQSN SNITRTIRE 
      HEASARNYV EKIEQTKDK IRDIEQKMN DQIAIIEQK 
      NQENEAMAS KIEELKVAQ RELLNQCGN DEEVQQKVR 
      KLTTEMQKF RRHVSELQN QRDEIVASR QTKSAQLRA 
      YENEINRID NVKQQRLNY LQRVDRDAY EAVVWLRNN 
      RNLFKGEIY EPIMLELNV LDSKNAMYV ENVIPLRDR 
      VAFTCTNKD DMNALIRLL RNEKQLTVN VLYAGDPND 
      HVSRYQPSI PIEQLRKYG LYAYMHSLF TAPEPIMRY 
      LCKTYRVHD IPIGNKQTN EFFQAVPSQ IRVFFSDKV 
      KYSVSYSRY TKAKSIRQN EIRSNGGFS ISVDVLQLE 
      RLRGQMQEL RNSFDTCDT QMKELDTQI SLINEKVSK 
      LGEEHKYIH SIKQQVQTI ETRIVAMQR KVQELQQQS 
      SNGDEIRAQ GRNKIRKVI RVLPQLSQE LKNTYESLT 
      VLTVKSELN FIKIEKARR HAAYLENKA VEARRLMEE 
      SEATLNQVK EAYANVKGQ AKAALQKAK GLSKGYTPG 
      DEAFEEFRE THERLSSDL RELQEEKLQ LNAKIECLS 
      TADDDEMRE YEERVETIK NLQGSIERA HVDLSKISA 
      KMDQLREEW LGPLGQLVT EINANFATA FERMGCAGE 
      VSINPGDDE QDFSNYGLR IKVTYRNGQ PMQELNSVV 
      QSGGERAVA TAVFMLALQ ELTPVPFRC VDEINQGMD 
      VNNERRIFD LLVESTSQA DTAQYFLIT PKLVPNLSY 
      SRSTMVHIV HNGPFVEPD RKWGMSKLC NPQRALIH
    CDS sequence
    • >TC008773 RA
      atgttcaaa gtgggcagt atacggaaa atcgaagtg 
      aagaacttt gtgacttac agctacgcg gagctgtac 
      ccgggcccc aatttgaac atgctgatt ggccccaac 
      ggcacgggt aagtctaca atcgtggct gctattatt 
      ttgggactt gggggtaac cccaaaaca gttggtaga 
      ggcgttagg gtgtctgaa tacgtgaaa cacaactgt 
      gaagaggcc acaattcat atttacttg caaggcaga 
      aaagacaat gattttatt aagattaca cgaattttt 
      aatacgcat gacaaaaca gggtggctt gtaaacaat 
      cagagagta acactgaaa gaagtaatg gattgcatc 
      aagcagtac aacatacaa gtcgataat ttgtgccag 
      tttttgccc caagaccgg gttcaggat ttcgcgaaa 
      atgaaccaa caacaatta ttgaaggaa actcaagta 
      gctctatgt cgaactgat ttaatagaa aagcaggag 
      gctttaata gcatgtaaa aataatcac aaacagctt 
      actgaagca atcgataag aacggagcg aagttgcag 
      gaaacccgc gacgctaat atgcgatta gaaggcaag 
      attgagaat tttagtagg aagaagaaa ttcttaagc 
      cagattgaa ggcatagac cgtaaaata gcctggtta 
      cagtatgat gatctctac gagaaaatg accgaaaca 
      aaagctgac ttggctaaa gcgaccaaa atttacgaa 
      aaacacaag agcgctact aaacctgcg gaaaaagaa 
      atccaaaag gccaaacaa gtggttcaa gaactgcaa 
      caatcaaac tcaaatatt acacgcacc attcgcgag 
      catgaagcg tccgcccga aattatgtg gagaaaatt 
      gagcaaacc aaagacaaa ataagggac attgagcag 
      aaaatgaat gatcagatt gccatcatt gagcaaaag 
      aatcaagaa aacgaagcc atggcttct aaaattgaa 
      gaattgaaa gtcgcgcaa agagaacta ctcaatcag 
      tgtggcaac gacgaggaa gttcaacaa aaagttcga 
      aaactaacg actgaaatg cagaaattt cgacgccac 
      gtgagcgaa ttgcagaat cagagagat gaaatcgtt 
      gcgtcaagg cagacgaag tcggcgcaa ttacgggct 
      tacgaaaac gaaatcaac agaattgat aatgtgaaa 
      caacaaagg ctaaattat ttgcagcgc gtggacagg 
      gatgcctat gaagcggtg gtttggctg aggaacaac 
      agaaatctt ttcaaggga gagatttat gagcctata 
      atgctagag ctcaacgtt cttgactcg aaaaatgcg 
      atgtacgtt gagaatgtc attccgctg cgtgaccgc 
      gtggccttt acttgcact aataaagac gatatgaat 
      gctttgatt cgccttttg agaaatgag aagcaactg 
      acagtcaac gtgctctat gctggtgat cccaatgac 
      cacgtatca cgttatcag ccttctatt cctatagag 
      cagttgcga aaatatggc ttgtacgct tacatgcac 
      tcactattt accgcccct gagccaata atgaggtac 
      ttatgtaag acgtaccga gtgcatgat attccaatc 
      ggcaataaa caaacaaac gagttcttc caagcagtg 
      ccttcccaa attagggtt ttctttagt gataaggtg 
      aaatattcg gtgagctac tcccgatac accaaagct 
      aaaagtata agacagaac gaaattaga tcaaacggg 
      ggtttctcc atatctgtt gatgtgtta caattggag 
      agacttcgt ggccaaatg caagagctt agaaatagt 
      ttcgacaca tgcgacact caaatgaaa gaacttgac 
      acacagatt tcgcttatt aatgagaaa gtttcaaaa 
      cttggggag gaacacaaa tatatccac agcatcaag 
      cagcaagtc caaacaatt gaaactcgt attgttgcc 
      atgcagcga aaggtgcaa gagttgcaa caacagtcg 
      tccaacggt gacgagatt cgcgcccaa gggcgcaac 
      aaaattcga aaagtcatc cgagttctt ccacaattg 
      tcccaagaa cttaaaaac acctacgag tccttaaca 
      gtcctcaca gttaagagc gaattgaac tttattaaa 
      attgagaaa gcgcgccgc catgccgcc tatttggaa 
      aacaaagct gtggaagcg cgacgcttg atggaggag 
      agtgaagcg accttaaac caggtcaag gaggcttac 
      gccaacgtg aagggtcaa gccaaagcg gcccttcag 
      aaggccaaa ggcttgtcc aaaggttat acacctggg 
      gatgaggct ttcgaggag tttcgggag acccacgag 
      cggctttct tccgacttg cgagagctt caagaggag 
      aagttgcaa ttaaacgcc aagattgag tgcttaagt 
      acagccgat gatgacgag atgcgcgag tatgaagaa 
      cgggtggag acgatcaaa aacctacaa gggagcatc 
      gaacgggct cacgtcgat ttgagcaaa atctcggcc 
      aagatggac caactgcgg gaggagtgg ttgggtccg 
      cttggccag ttggtcact gagattaac gccaacttc 
      gccacggct ttcgaacgg atgggctgt gcgggggaa 
      gtctccata aacccgggc gatgacgag caagacttc 
      tccaattac gggctgcgt atcaaagtg acctatcgc 
      aatggacag ccgatgcaa gaactcaac agtgtggtt 
      caaagtgga ggagagagg gctgtggcg acggcagtg 
      tttatgttg gcgttgcaa gaattgact ccagtgcct 
      tttcgatgc gtcgatgag attaatcaa gggatggat 
      gtgaataat gaacggagg attttcgat ttgttggtg 
      gagtcgacc agtcaggcc gacacggcg cagtatttt 
      ttgatcacg ccgaagcta gtacccaac ttgagctac 
      tcgaggtcg acgatggtg catattgtg cacaacgga 
      ccgtttgtg gagccggac aggaagtgg ggcatgtcc 
      aaattgtgt aacccgcaa agagcgcta atacattaa
    mRNA sequence
    • >TC008773 RA
      gcgggaaac tacagtatc gaggttatg tttgtgtat 
      ttgttatca attaatagt aattattat tagcaatgt 
      tcaaagtgg gcagtatac ggaaaatcg aagtgaaga 
      actttgtga cttacagct acgcggagc tgtacccgg 
      gccccaatt tgaacatgc tgattggcc ccaacggca 
      cgggtaagt ctacaatcg tggctgcta ttattttgg 
      gacttgggg gtaacccca aaacagttg gtagaggcg 
      ttagggtgt ctgaatacg tgaaacaca actgtgaag 
      aggccacaa ttcatattt acttgcaag gcagaaaag 
      acaatgatt ttattaaga ttacacgaa tttttaata 
      cgcatgaca aaacagggt ggcttgtaa acaatcaga 
      gagtaacac tgaaagaag taatggatt gcatcaagc 
      agtacaaca tacaagtcg ataatttgt gccagtttt 
      tgccccaag accgggttc aggatttcg cgaaaatga 
      accaacaac aattattga aggaaactc aagtagctc 
      tatgtcgaa ctgatttaa tagaaaagc aggaggctt 
      taatagcat gtaaaaata atcacaaac agcttactg 
      aagcaatcg ataagaacg gagcgaagt tgcaggaaa 
      cccgcgacg ctaatatgc gattagaag gcaagattg 
      agaatttta gtaggaaga agaaattct taagccaga 
      ttgaaggca tagaccgta aaatagcct ggttacagt 
      atgatgatc tctacgaga aaatgaccg aaacaaaag 
      ctgacttgg ctaaagcga ccaaaattt acgaaaaac 
      acaagagcg ctactaaac ctgcggaaa aagaaatcc 
      aaaaggcca aacaagtgg ttcaagaac tgcaacaat 
      caaactcaa atattacac gcaccattc gcgagcatg 
      aagcgtccg cccgaaatt atgtggaga aaattgagc 
      aaaccaaag acaaaataa gggacattg agcagaaaa 
      tgaatgatc agattgcca tcattgagc aaaagaatc 
      aagaaaacg aagccatgg cttctaaaa ttgaagaat 
      tgaaagtcg cgcaaagag aactactca atcagtgtg 
      gcaacgacg aggaagttc aacaaaaag ttcgaaaac 
      taacgactg aaatgcaga aatttcgac gccacgtga 
      gcgaattgc agaatcaga gagatgaaa tcgttgcgt 
      caaggcaga cgaagtcgg cgcaattac gggcttacg 
      aaaacgaaa tcaacagaa ttgataatg tgaaacaac 
      aaaggctaa attatttgc agcgcgtgg acagggatg 
      cctatgaag cggtggttt ggctgagga acaacagaa 
      atcttttca agggagaga tttatgagc ctataatgc 
      tagagctca acgttcttg actcgaaaa atgcgatgt 
      acgttgaga atgtcattc cgctgcgtg accgcgtgg 
      cctttactt gcactaata aagacgata tgaatgctt 
      tgattcgcc ttttgagaa atgagaagc aactgacag 
      tcaacgtgc tctatgctg gtgatccca atgaccacg 
      tatcacgtt atcagcctt ctattccta tagagcagt 
      tgcgaaaat atggcttgt acgcttaca tgcactcac 
      tatttaccg cccctgagc caataatga ggtacttat 
      gtaagacgt accgagtgc atgatattc caatcggca 
      ataaacaaa caaacgagt tcttccaag cagtgcctt 
      cccaaatta gggttttct ttagtgata aggtgaaat 
      attcggtga gctactccc gatacacca aagctaaaa 
      gtataagac agaacgaaa ttagatcaa acgggggtt 
      tctccatat ctgttgatg tgttacaat tggagagac 
      ttcgtggcc aaatgcaag agcttagaa atagtttcg 
      acacatgcg acactcaaa tgaaagaac ttgacacac 
      agatttcgc ttattaatg agaaagttt caaaacttg 
      gggaggaac acaaatata tccacagca tcaagcagc 
      aagtccaaa caattgaaa ctcgtattg ttgccatgc 
      agcgaaagg tgcaagagt tgcaacaac agtcgtcca 
      acggtgacg agattcgcg cccaagggc gcaacaaaa 
      ttcgaaaag tcatccgag ttcttccac aattgtccc 
      aagaactta aaaacacct acgagtcct taacagtcc 
      tcacagtta agagcgaat tgaacttta ttaaaattg 
      agaaagcgc gccgccatg ccgcctatt tggaaaaca 
      aagctgtgg aagcgcgac gcttgatgg aggagagtg 
      aagcgacct taaaccagg tcaaggagg cttacgcca 
      acgtgaagg gtcaagcca aagcggccc ttcagaagg 
      ccaaaggct tgtccaaag gttatacac ctggggatg 
      aggctttcg aggagtttc gggagaccc acgagcggc 
      tttcttccg acttgcgag agcttcaag aggagaagt 
      tgcaattaa acgccaaga ttgagtgct taagtacag 
      ccgatgatg acgagatgc gcgagtatg aagaacggg 
      tggagacga tcaaaaacc tacaaggga gcatcgaac 
      gggctcacg tcgatttga gcaaaatct cggccaaga 
      tggaccaac tgcgggagg agtggttgg gtccgcttg 
      gccagttgg tcactgaga ttaacgcca acttcgcca 
      cggctttcg aacggatgg gctgtgcgg gggaagtct 
      ccataaacc cgggcgatg acgagcaag acttctcca 
      attacgggc tgcgtatca aagtgacct atcgcaatg 
      gacagccga tgcaagaac tcaacagtg tggttcaaa 
      gtggaggag agagggctg tggcgacgg cagtgttta 
      tgttggcgt tgcaagaat tgactccag tgccttttc 
      gatgcgtcg atgagatta atcaaggga tggatgtga 
      ataatgaac ggaggattt tcgatttgt tggtggagt 
      cgaccagtc aggccgaca cggcgcagt attttttga 
      tcacgccga agctagtac ccaacttga gctactcga 
      ggtcgacga tggtgcata ttgtgcaca acggaccgt 
      ttgtggagc cggacagga agtggggca tgtccaaat 
      tgtgtaacc cgcaaagag cgctaatac attaattgt 
      tttgtttgt gtttttgtt ttatatttt cacagatac 
      tatgttttt actaaataa agattcttt cttaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY