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Gene information
General information
TC008761 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9609397..9611367
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008761
    iBeetle screen iB_01409

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008761 RA
      MWIFLLITL CTICNGLAY KAAVVEYYP STEAQPMDT 
      IKKNIEEYR TYVDSARKQ SVDIIVFPE YGLTTLTKD 
      PEEYAVEIT STNEIISKL STLAKEHEM YLVINLLEK 
      ETIANQIYY YNTNLVFDR NGTTIAKYR KINLFDEAK 
      LTPGSHNQT NTFLTRLWG HFWDIYLFR HLVTDIVYP 
      TAWISIIPF YHSLSVQHG YAVANGVNL LAANYAKPN 
      AGRGGSGIY LTDGKIAEK YIGDTASTK LIVQEVGKQ 
      STREDRTKC PTRLPFGLP SDLGKSNVS NYMALTAFT 
      ASDYTFQNI NLTQGNISE TVCHKNFCC NFDLKLDPN 
      NVIASEHYK LMAYDGMVN YNEIQLHIR TCSLLFCEN 
      DSNDSCGKR QETTSTKFT KITVSGNLP TDNTTFYTP 
      VTLNTYLLT IPQIPYCDT QNGTDTTYV QLSTPKAQQ 
      NLLVFGLLG HTQAPIDDH NDNGDDDDG NSAVKASSL 
      LLLVVLSVV KCLLY
    CDS sequence
    • >TC008761 RA
      atgtggatc ttcttatta ataacactt tgtaccatt 
      tgtaatgga cttgcgtac aaagctgca gttgtcgag 
      tattatcct tcaacagaa gcccaaccc atggacaca 
      atcaaaaag aatatagaa gagtatagg acgtacgta 
      gacagcgca agaaaacaa tctgtcgat atcatagtg 
      ttcccagaa tatggtctt acgacttta actaaagat 
      ccagaagaa tatgccgtt gaaataact agtactaat 
      gaaatcatt tcaaagctt agcactttg gccaaagaa 
      catgagatg tacctagtc ataaatctt ctagaaaaa 
      gaaacgatc gccaaccaa atatactac tacaacaca 
      aatttggtt tttgatcgg aatggcaca accatagcc 
      aaataccgc aaaatcaat ctcttcgac gaggctaaa 
      ctgactcca ggatctcac aaccaaacc aacactttt 
      ctgacccga ctttggggt cacttttgg gatatttac 
      ttgtttcga catcttgtc actgatatt gtttatcca 
      acagcgtgg atttctata atccccttc tatcattct 
      ttgagtgtc caacacggg tatgcggtc gctaacgga 
      gttaatctt ctggccgct aattacgca aaaccgaac 
      gcgggccgc ggtggtagc ggtatatat ttaactgat 
      gggaaaata gcggagaaa tacattgga gatacagca 
      agtactaaa cttatagtc caagaagtc gggaagcaa 
      tcgactaga gaagacaga acgaaatgt ccaactcga 
      ttaccgttt ggacttccg tcagattta ggaaaatct 
      aacgtcagt aactatatg gcactcaca gctttcact 
      gcatcagat tacacattc caaaacatt aatttgaca 
      cagggtaac atctccgaa acagtttgc cacaaaaac 
      ttttgttgc aattttgac ctgaaacta gatccaaat 
      aacgtcatt gcttcagaa cactacaaa ctaatggca 
      tatgatgga atggtaaat tataacgaa attcagctt 
      catatccgt acctgcagt ctattattt tgtgaaaat 
      gattccaac gacagttgt ggtaaaaga caagaaacg 
      acatccacc aagttcaca aaaataaca gtcagtgga 
      aatctgccc accgataat acgactttt tatacaccc 
      gtcacgctt aacacttat cttttaaca ataccgcaa 
      attccatac tgtgacact caaaacgga actgatacc 
      acctatgtg cagttgtct acgccaaaa gcgcagcaa 
      aatcttcta gtttttggt ttgcttgga cacactcag 
      gctccaatt gatgatcac aatgacaac ggagatgac 
      gatgatggc aattcagca gtgaaagct tccagtctt 
      ctcttactt gtagttttg agtgttgtt aaatgtttg 
      ctgtattaa
    mRNA sequence
    • >TC008761 RA
      tgttaccta cacacctaa caaaatagt tgcaagctg 
      tgtcactct tcgtcctta aaatgtgga tcttcttat 
      taataacac tttgtacca tttgtaatg gacttgcgt 
      acaaagctg cagttgtcg agtattatc cttcaacag 
      aagcccaac ccatggaca caatcaaaa agaatatag 
      aagagtata ggacgtacg tagacagcg caagaaaac 
      aatctgtcg atatcatag tgttcccag aatatggtc 
      ttacgactt taactaaag atccagaag aatatgccg 
      ttgaaataa ctagtacta atgaaatca tttcaaagc 
      ttagcactt tggccaaag aacatgaga tgtacctag 
      tcataaatc ttctagaaa aagaaacga tcgccaacc 
      aaatatact actacaaca caaatttgg tttttgatc 
      ggaatggca caaccatag ccaaatacc gcaaaatca 
      atctcttcg acgaggcta aactgactc caggatctc 
      acaaccaaa ccaacactt ttctgaccc gactttggg 
      gtcactttt gggatattt acttgtttc gacatcttg 
      tcactgata ttgtttatc caacagcgt ggatttcta 
      taatcccct tctatcatt ctttgagtg tccaacacg 
      ggtatgcgg tcgctaacg gagttaatc ttctggccg 
      ctaattacg caaaaccga acgcgggcc gcggtggta 
      gcggtatat atttaactg atgggaaaa tagcggaga 
      aatacattg gagatacag caagtacta aacttatag 
      tccaagaag tcgggaagc aatcgacta gagaagaca 
      gaacgaaat gtccaactc gattaccgt ttggacttc 
      cgtcagatt taggaaaat ctaacgtca gtaactata 
      tggcactca cagctttca ctgcatcag attacacat 
      tccaaaaca ttaatttga cacagggta acatctccg 
      aaacagttt gccacaaaa acttttgtt gcaattttg 
      acctgaaac tagatccaa ataacgtca ttgcttcag 
      aacactaca aactaatgg catatgatg gaatggtaa 
      attataacg aaattcagc ttcatatcc gtacctgca 
      gtctattat tttgtgaaa atgattcca acgacagtt 
      gtggtaaaa gacaagaaa cgacatcca ccaagttca 
      caaaaataa cagtcagtg gaaatctgc ccaccgata 
      atacgactt tttatacac ccgtcacgc ttaacactt 
      atcttttaa caataccgc aaattccat actgtgaca 
      ctcaaaacg gaactgata ccacctatg tgcagttgt 
      ctacgccaa aagcgcagc aaaatcttc tagtttttg 
      gtttgcttg gacacactc aggctccaa ttgatgatc 
      acaatgaca acggagatg acgatgatg gcaattcag 
      cagtgaaag cttccagtc ttctcttac ttgtagttt 
      tgagtgttg ttaaatgtt tgctgtatt aattacact 
      aggaattta attttgtag aagcgaggt aaaaaataa 
      atcctaaat gcctttcac tttttcacg taaggaaca 
      attaattgc tccttattt tgatccaaa aaattggta 
      ataaaagta ataatacaa a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY