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Gene information
General information
TC008760 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid49: 2937170..2941436
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008760
    iBeetle screen iB_01408
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008760 RA
      MRFISVFVS VILFNTFRL STAYKAAVF EHYALQADT 
      PENTILKNL DEYRNHADK AKIQAADIA VFPEYGLTT 
      VILDNPEEY AVVVNSTNH IINELMTIA KERAIYLVV 
      NLLEKEEEA NKKTKYYNT NLVFDRDGK IILKYRKIN 
      LFNEGKLTA GPKDQTPTF TTDFGVTFG IFTCFDILF 
      ENPSRTVLK NDAVTDIVF PTAWFATMP FFTSLSIQH 
      GYAAANGVN LLAANFNNP KETHGGSGI YLGDGRVVE 
      MVVFDTPSS KPLIQEVPK LSKREDKSI CPKLSPFGL 
      PNDLTKSNV SNYHTDYEF KASNYKLKK IDLTQSNVT 
      TTICDSTFC CNFEIIIST SETNTSSVY KVMAYDGPV 
      SYGNVNAHI RVCSLVACE SDSDTSCGK RVDVGVKFT 
      KITVKGDLI TDETYPTYY TPLTLNTYL QPLTQTIYC 
      NNKNSGSNS TSVQLTTTK AQKDVLVFG ILGTTTASA 
      AAPSGANII VFPEYGLTT LVDDPKEYA INVNDSDTF 
      KKLKVMAVE NGMYLVVNI LEKDESSSK NTEYYNTNL 
      VFDRNGELL LKYRKINLF NEKQLTPGS KDQSNTFKT 
      DFGVTFGIF TSFDILFEN PSKTVFIDT SVTDIVFPT 
      AWFATMPFF TALSVQHGY AVANGVNLL AANYGNPKS 
      THGGSGIYL GDGKVPEMY IADTPSSKA IVQEVPKLS 
      KREDKTICP KVAPRGLPS DLDKSNVTN YRTDYVFKA 
      SDYTFKKID MTQSNATAS VCDGSLCCT FQMHINTSE 
      SKSINVYKV MAYDGPMTY GNSNIHVRV CSLVACETD 
      SDDTCGKRV EVDAKFTKI TVKGKLTSC NEGPTYFAP 
      VTLNTFLQP LTETTYCNF NATDSTNVE LTTTKAQQN 
      VLVFGILGQ VSASASLFT FSVLFLTML SVIKCIVV
    • >TC008760 RB
      MKGVCVLFV TFLGFNFSA AYKAAVIEH YPYADKGST 
      ADEIIKKNL QDYQNYIAK AKGEGANII VFPEYGLTT 
      LVDDPKEYA INVNDSDTF KKLKVMAVE NGMYLVVNI 
      LEKDESSSK NTEYYNTNL VFDRNGELL LKYRKINLF 
      NEKQLTPGS KDQSNTFKT DFGVTFGIF TSFDILFEN 
      PSKTVFIDT SVTDIVFPT AWFATMPFF TALSVQHGY 
      AVANGVNLL AANYGNPKS THGGSGIYL GDGKVPEMY 
      IADTPSSKA IVQEVPKLS KREDKTICP KVAPRGLPS 
      DLDKSNVTN YRTDYVFKA SDYTFKKID MTQSNATAS 
      VCDGSLCCT FQMHINTSE SKSINVYKV MAYDGPMTY 
      GNSNIHVRV CSLVACETD SDDTCGKRV EVDAKFTKI 
      TVKGKLTSC NEGPTYFAP VTLNTFLQP LTETTYCNF 
      NATDSTNVE LTTTKAQQN VLVFGILGQ VSASASLFT 
      FSVLFLTML SVIKCIVV
    • >TC008760 RC
      MRFISVFVS VILFNTFRL STAYKAAVF EHYALQADT 
      PENTILKNL DEYRNHADK AKIQAADIA VFPEYGLTT 
      VILDNPEEY AVVVNSTNH IINELMTIA KERAIYLVV 
      NLLEKEEEA NKKTKYYNT NLVFDRDGK IILKYRKIN 
      LFNEGKLTA GPKDQTPTF TTDFGVTFG IFTCFDILF 
      ENPSRTVLK NDAVTDIVF PTAWFATMP FFTSLSIQH 
      GYAAANGVN LLAANFNNP KETHGGSGI YLGDGRVVE 
      MVVFDTPSS KPLIQEVPK LSKREDKSI CPKLSPFGL 
      PNDLTKSNV SNYHTDYEF KASNYKLKK IDLTQSNVT 
      TTICDSTFC CNFEIIIST SETNTSSVY KVMAYDGPV 
      SYGNVNAHI RVCSLVACE SDSDTSCGK RVDVGVKFT 
      KITVKGDLI TDETYPTYY TPLTLNTYL QPLTQTIYC 
      NNKNSGSNS TSVQLTTTK AQKDVLVFG ILGTTTASA 
      AAPSPN
    CDS sequence
    • >TC008760 RA
      atgagattt ataagtgtg tttgttagc gttatttta 
      tttaacact ttcagattg tcaacagcc tacaaagct 
      gcagttttc gaacattat gcgctacaa gccgatact 
      ccagaaaac actatcttg aaaaatctt gacgaatat 
      agaaatcat gcagataaa gctaaaatt caggctgcg 
      gatattgct gtctttccg gaatatggt cttacgaca 
      gtaatactg gacaatcca gaggaatat gcagttgtt 
      gtcaacagc acaaaccat attataaac gaactaatg 
      accattgcc aaagagcgg gccatttat ctagttgta 
      aacttactg gaaaaagag gaggaagcc aacaaaaag 
      actaaatat tacaacaca aatttagtc ttcgacaga 
      gatggaaaa ataatacta aaataccgc aaaatcaat 
      ctcttcaat gaaggaaaa cttaccgca ggcccgaaa 
      gatcaaacc cctacattt accacagat ttcggtgtt 
      acgtttggc attttcaca tgctttgat attcttttc 
      gaaaacccg tcgagaact gtactaaag aatgacgct 
      gttacggat attgtgttc cccacagct tggtttgca 
      acaatgcct ttctttaca tctttgagc atccaacat 
      ggttacgct gcagctaac ggcgtgaat ttacttgct 
      gctaacttt aataatccg aaagaaacc catggtggt 
      agtgggatc tacttagga gatggtaga gttgtggaa 
      atggtcgtt tttgacact ccaagcagt aaaccttta 
      atccaggag gtaccaaaa ttatccaaa cgagaagac 
      aaaagcatc tgtcctaaa ctttcccca tttggcctt 
      ccaaatgat ttaactaaa tccaatgtt agcaattat 
      cacacagac tacgagttt aaagcttca aactacaag 
      ctcaagaaa attgatttg acccaaagc aatgttaca 
      accaccatt tgtgactcc acgttttgt tgcaatttt 
      gaaattatt attagtact tcagaaaca aacacaagc 
      agtgtctac aaggtaatg gcttatgac ggacctgtt 
      tcttacggt aatgttaac gctcatatt cgagtgtgt 
      agtttggta gcttgcgaa agtgactca gacacgagt 
      tgtggaaaa agagttgat gttggtgtc aagtttacg 
      aaaattaca gttaaagga gatttgata actgatgaa 
      acctaccca acttactac acacctttg actctaaat 
      acctatctt cagccacta acgcaaacg atttattgt 
      aacaataag aatagtgga agcaactct acttctgtc 
      caactaacc acaacaaag gcgcaaaag gacgtctta 
      gtttttggg attttggga acaacaaca gcatctgca 
      gctgcacct tcgggcgca aatatcatt gtctttcct 
      gaatatggg cttacaact ctagtcgac gacccaaag 
      gaatatgct atcaatgtt aatgactcc gatacgttt 
      aaaaaactg aaagtaatg gctgtagaa aacggcatg 
      tatttggtg gtaaatatt ctagaaaaa gatgaaagc 
      agtagcaaa aacactgaa tattacaac acaaacttg 
      gttttcgac agaaacgga gaattatta ttaaaatac 
      cgcaaaatc aatcttttt aacgaaaaa caattgaca 
      ccaggatca aaagatcaa agcaacact tttaaaact 
      gattttggc gtcacgttt ggtattttc acaagtttt 
      gatatcctt tttgaaaac ccgtcgaaa actgtattc 
      attgatacc agtgttacg gatatcgtg tttcccaca 
      gcttggttt gcaacaatg cctttcttt actgctttg 
      agcgtccaa catggttac gctgtcgcc aacggtgtt 
      aatttatta gctgctaat tatggtaat ccaaaaagc 
      acccacggg ggcagtgga atttacttg ggagatggc 
      aaggtgcct gaaatgtat attgctgat actccaagc 
      agtaaagct atagtccaa gaagtacca aaattatcc 
      aaacgggaa gacaaaaca atctgtcct aaagttgcc 
      ccaagagga ctcccaagt gatttagat aaatctaat 
      gttaccaac taccgcaca gactacgtc tttaaagcc 
      tcagactac acctttaaa aaaattgat atgacccaa 
      agcaatgct acagctagc gtttgtgat ggctcgttg 
      tgttgcact ttccaaatg cacataaat acatcagaa 
      tcaaaatca atcaacgtt tataaggtg atggcttat 
      gacggtcca atgacttac ggtaacagc aacattcat 
      gttcgcgtg tgcagtttg gtggcttgc gaaaccgac 
      tcagatgat acttgtggg aaaagagtc gaggttgat 
      gctaaattt acaaaaatt acagttaag ggaaaattg 
      acaagttgt aatgagggc cctacttat tttgcgcct 
      gtcactcta aacactttt cttcaacct ctaaccgaa 
      accacatat tgtaacttc aacgcaact gattctaca 
      aacgtcgaa ttgactacg actaaagcc caacaaaat 
      gttttagtt tttgggatc ttgggacaa gtatcagcc 
      agcgcctct ctatttact ttcagtgtc ttgttcttg 
      accatgtta agtgttatt aaatgtatt gtagtttag
    • >TC008760 RB
      atgaagggt gtgtgcgta ttgtttgtt acattttta 
      ggattcaat ttttctgca gcctacaaa gcggcagtt 
      attgaacat tatccgtat gcagataaa ggaagtaca 
      gcagatgaa ataataaaa aaaaatcta caggactat 
      caaaattac atagcgaaa gcgaaaggc gaaggcgca 
      aatatcatt gtctttcct gaatatggg cttacaact 
      ctagtcgac gacccaaag gaatatgct atcaatgtt 
      aatgactcc gatacgttt aaaaaactg aaagtaatg 
      gctgtagaa aacggcatg tatttggtg gtaaatatt 
      ctagaaaaa gatgaaagc agtagcaaa aacactgaa 
      tattacaac acaaacttg gttttcgac agaaacgga 
      gaattatta ttaaaatac cgcaaaatc aatcttttt 
      aacgaaaaa caattgaca ccaggatca aaagatcaa 
      agcaacact tttaaaact gattttggc gtcacgttt 
      ggtattttc acaagtttt gatatcctt tttgaaaac 
      ccgtcgaaa actgtattc attgatacc agtgttacg 
      gatatcgtg tttcccaca gcttggttt gcaacaatg 
      cctttcttt actgctttg agcgtccaa catggttac 
      gctgtcgcc aacggtgtt aatttatta gctgctaat 
      tatggtaat ccaaaaagc acccacggg ggcagtgga 
      atttacttg ggagatggc aaggtgcct gaaatgtat 
      attgctgat actccaagc agtaaagct atagtccaa 
      gaagtacca aaattatcc aaacgggaa gacaaaaca 
      atctgtcct aaagttgcc ccaagagga ctcccaagt 
      gatttagat aaatctaat gttaccaac taccgcaca 
      gactacgtc tttaaagcc tcagactac acctttaaa 
      aaaattgat atgacccaa agcaatgct acagctagc 
      gtttgtgat ggctcgttg tgttgcact ttccaaatg 
      cacataaat acatcagaa tcaaaatca atcaacgtt 
      tataaggtg atggcttat gacggtcca atgacttac 
      ggtaacagc aacattcat gttcgcgtg tgcagtttg 
      gtggcttgc gaaaccgac tcagatgat acttgtggg 
      aaaagagtc gaggttgat gctaaattt acaaaaatt 
      acagttaag ggaaaattg acaagttgt aatgagggc 
      cctacttat tttgcgcct gtcactcta aacactttt 
      cttcaacct ctaaccgaa accacatat tgtaacttc 
      aacgcaact gattctaca aacgtcgaa ttgactacg 
      actaaagcc caacaaaat gttttagtt tttgggatc 
      ttgggacaa gtatcagcc agcgcctct ctatttact 
      ttcagtgtc ttgttcttg accatgtta agtgttatt 
      aaatgtatt gtagtttag
    • >TC008760 RC
      atgagattt ataagtgtg tttgttagc gttatttta 
      tttaacact ttcagattg tcaacagcc tacaaagct 
      gcagttttc gaacattat gcgctacaa gccgatact 
      ccagaaaac actatcttg aaaaatctt gacgaatat 
      agaaatcat gcagataaa gctaaaatt caggctgcg 
      gatattgct gtctttccg gaatatggt cttacgaca 
      gtaatactg gacaatcca gaggaatat gcagttgtt 
      gtcaacagc acaaaccat attataaac gaactaatg 
      accattgcc aaagagcgg gccatttat ctagttgta 
      aacttactg gaaaaagag gaggaagcc aacaaaaag 
      actaaatat tacaacaca aatttagtc ttcgacaga 
      gatggaaaa ataatacta aaataccgc aaaatcaat 
      ctcttcaat gaaggaaaa cttaccgca ggcccgaaa 
      gatcaaacc cctacattt accacagat ttcggtgtt 
      acgtttggc attttcaca tgctttgat attcttttc 
      gaaaacccg tcgagaact gtactaaag aatgacgct 
      gttacggat attgtgttc cccacagct tggtttgca 
      acaatgcct ttctttaca tctttgagc atccaacat 
      ggttacgct gcagctaac ggcgtgaat ttacttgct 
      gctaacttt aataatccg aaagaaacc catggtggt 
      agtgggatc tacttagga gatggtaga gttgtggaa 
      atggtcgtt tttgacact ccaagcagt aaaccttta 
      atccaggag gtaccaaaa ttatccaaa cgagaagac 
      aaaagcatc tgtcctaaa ctttcccca tttggcctt 
      ccaaatgat ttaactaaa tccaatgtt agcaattat 
      cacacagac tacgagttt aaagcttca aactacaag 
      ctcaagaaa attgatttg acccaaagc aatgttaca 
      accaccatt tgtgactcc acgttttgt tgcaatttt 
      gaaattatt attagtact tcagaaaca aacacaagc 
      agtgtctac aaggtaatg gcttatgac ggacctgtt 
      tcttacggt aatgttaac gctcatatt cgagtgtgt 
      agtttggta gcttgcgaa agtgactca gacacgagt 
      tgtggaaaa agagttgat gttggtgtc aagtttacg 
      aaaattaca gttaaagga gatttgata actgatgaa 
      acctaccca acttactac acacctttg actctaaat 
      acctatctt cagccacta acgcaaacg atttattgt 
      aacaataag aatagtgga agcaactct acttctgtc 
      caactaacc acaacaaag gcgcaaaag gacgtctta 
      gtttttggg attttggga acaacaaca gcatctgca 
      gctgcacct tcgcctaat taa
    mRNA sequence
    • >TC008760 RA
      agagtacaa taataattt ttatcactt atcagatta 
      atctctcat tctctcacc tgttcactt tttactaga 
      acgtgcact gttgtgtcc aaaaatgag atttataag 
      tgtgtttgt tagcgttat tttatttaa cactttcag 
      attgtcaac agcctacaa agctgcagt tttcgaaca 
      ttatgcgct acaagccga tactccaga aaacactat 
      cttgaaaaa tcttgacga atatagaaa tcatgcaga 
      taaagctaa aattcaggc tgcggatat tgctgtctt 
      tccggaata tggtcttac gacagtaat actggacaa 
      tccagagga atatgcagt tgttgtcaa cagcacaaa 
      ccatattat aaacgaact aatgaccat tgccaaaga 
      gcgggccat ttatctagt tgtaaactt actggaaaa 
      agaggagga agccaacaa aaagactaa atattacaa 
      cacaaattt agtcttcga cagagatgg aaaaataat 
      actaaaata ccgcaaaat caatctctt caatgaagg 
      aaaacttac cgcaggccc gaaagatca aacccctac 
      atttaccac agatttcgg tgttacgtt tggcatttt 
      cacatgctt tgatattct tttcgaaaa cccgtcgag 
      aactgtact aaagaatga cgctgttac ggatattgt 
      gttccccac agcttggtt tgcaacaat gcctttctt 
      tacatcttt gagcatcca acatggtta cgctgcagc 
      taacggcgt gaatttact tgctgctaa ctttaataa 
      tccgaaaga aacccatgg tggtagtgg gatctactt 
      aggagatgg tagagttgt ggaaatggt cgtttttga 
      cactccaag cagtaaacc tttaatcca ggaggtacc 
      aaaattatc caaacgaga agacaaaag catctgtcc 
      taaactttc cccatttgg ccttccaaa tgatttaac 
      taaatccaa tgttagcaa ttatcacac agactacga 
      gtttaaagc ttcaaacta caagctcaa gaaaattga 
      tttgaccca aagcaatgt tacaaccac catttgtga 
      ctccacgtt ttgttgcaa ttttgaaat tattattag 
      tacttcaga aacaaacac aagcagtgt ctacaaggt 
      aatggctta tgacggacc tgtttctta cggtaatgt 
      taacgctca tattcgagt gtgtagttt ggtagcttg 
      cgaaagtga ctcagacac gagttgtgg aaaaagagt 
      tgatgttgg tgtcaagtt tacgaaaat tacagttaa 
      aggagattt gataactga tgaaaccta cccaactta 
      ctacacacc tttgactct aaataccta tcttcagcc 
      actaacgca aacgattta ttgtaacaa taagaatag 
      tggaagcaa ctctacttc tgtccaact aaccacaac 
      aaaggcgca aaaggacgt cttagtttt tgggatttt 
      gggaacaac aacagcatc tgcagctgc accttcggg 
      cgcaaatat cattgtctt tcctgaata tgggcttac 
      aactctagt cgacgaccc aaaggaata tgctatcaa 
      tgttaatga ctccgatac gtttaaaaa actgaaagt 
      aatggctgt agaaaacgg catgtattt ggtggtaaa 
      tattctaga aaaagatga aagcagtag caaaaacac 
      tgaatatta caacacaaa cttggtttt cgacagaaa 
      cggagaatt attattaaa ataccgcaa aatcaatct 
      ttttaacga aaaacaatt gacaccagg atcaaaaga 
      tcaaagcaa cacttttaa aactgattt tggcgtcac 
      gtttggtat tttcacaag ttttgatat cctttttga 
      aaacccgtc gaaaactgt attcattga taccagtgt 
      tacggatat cgtgtttcc cacagcttg gtttgcaac 
      aatgccttt ctttactgc tttgagcgt ccaacatgg 
      ttacgctgt cgccaacgg tgttaattt attagctgc 
      taattatgg taatccaaa aagcaccca cgggggcag 
      tggaattta cttgggaga tggcaaggt gcctgaaat 
      gtatattgc tgatactcc aagcagtaa agctatagt 
      ccaagaagt accaaaatt atccaaacg ggaagacaa 
      aacaatctg tcctaaagt tgccccaag aggactccc 
      aagtgattt agataaatc taatgttac caactaccg 
      cacagacta cgtctttaa agcctcaga ctacacctt 
      taaaaaaat tgatatgac ccaaagcaa tgctacagc 
      tagcgtttg tgatggctc gttgtgttg cactttcca 
      aatgcacat aaatacatc agaatcaaa atcaatcaa 
      cgtttataa ggtgatggc ttatgacgg tccaatgac 
      ttacggtaa cagcaacat tcatgttcg cgtgtgcag 
      tttggtggc ttgcgaaac cgactcaga tgatacttg 
      tgggaaaag agtcgaggt tgatgctaa atttacaaa 
      aattacagt taagggaaa attgacaag ttgtaatga 
      gggccctac ttattttgc gcctgtcac tctaaacac 
      ttttcttca acctctaac cgaaaccac atattgtaa 
      cttcaacgc aactgattc tacaaacgt cgaattgac 
      tacgactaa agcccaaca aaatgtttt agtttttgg 
      gatcttggg acaagtatc agccagcgc ctctctatt 
      tactttcag tgtcttgtt cttgaccat gttaagtgt 
      tattaaatg tattgtagt ttagttaag acagccttg 
      taagcatta aatttaata aaacctaat attcgta
    • >TC008760 RB
      tgagcatat taatgtaat tactggttt aacttcagg 
      ctgctacaa catgaaggg tgtgtgcgt attgtttgt 
      tacattttt aggattcaa tttttctgc agcctacaa 
      agcggcagt tattgaaca ttatccgta tgcagataa 
      aggaagtac agcagatga aataataaa aaaaaatct 
      acaggacta tcaaaatta catagcgaa agcgaaagg 
      cgaaggcgc aaatatcat tgtctttcc tgaatatgg 
      gcttacaac tctagtcga cgacccaaa ggaatatgc 
      tatcaatgt taatgactc cgatacgtt taaaaaact 
      gaaagtaat ggctgtaga aaacggcat gtatttggt 
      ggtaaatat tctagaaaa agatgaaag cagtagcaa 
      aaacactga atattacaa cacaaactt ggttttcga 
      cagaaacgg agaattatt attaaaata ccgcaaaat 
      caatctttt taacgaaaa acaattgac accaggatc 
      aaaagatca aagcaacac ttttaaaac tgattttgg 
      cgtcacgtt tggtatttt cacaagttt tgatatcct 
      ttttgaaaa cccgtcgaa aactgtatt cattgatac 
      cagtgttac ggatatcgt gtttcccac agcttggtt 
      tgcaacaat gcctttctt tactgcttt gagcgtcca 
      acatggtta cgctgtcgc caacggtgt taatttatt 
      agctgctaa ttatggtaa tccaaaaag cacccacgg 
      gggcagtgg aatttactt gggagatgg caaggtgcc 
      tgaaatgta tattgctga tactccaag cagtaaagc 
      tatagtcca agaagtacc aaaattatc caaacggga 
      agacaaaac aatctgtcc taaagttgc cccaagagg 
      actcccaag tgatttaga taaatctaa tgttaccaa 
      ctaccgcac agactacgt ctttaaagc ctcagacta 
      cacctttaa aaaaattga tatgaccca aagcaatgc 
      tacagctag cgtttgtga tggctcgtt gtgttgcac 
      tttccaaat gcacataaa tacatcaga atcaaaatc 
      aatcaacgt ttataaggt gatggctta tgacggtcc 
      aatgactta cggtaacag caacattca tgttcgcgt 
      gtgcagttt ggtggcttg cgaaaccga ctcagatga 
      tacttgtgg gaaaagagt cgaggttga tgctaaatt 
      tacaaaaat tacagttaa gggaaaatt gacaagttg 
      taatgaggg ccctactta ttttgcgcc tgtcactct 
      aaacacttt tcttcaacc tctaaccga aaccacata 
      ttgtaactt caacgcaac tgattctac aaacgtcga 
      attgactac gactaaagc ccaacaaaa tgttttagt 
      ttttgggat cttgggaca agtatcagc cagcgcctc 
      tctatttac tttcagtgt cttgttctt gaccatgtt 
      aagtgttat taaatgtat tgtagttta gttaagaca 
      gccttgtaa gcattaaat ttaataaaa cctaatatt 
      cgta
    • >TC008760 RC
      agagtacaa taataattt ttatcactt atcagatta 
      atctctcat tctctcacc tgttcactt tttactaga 
      acgtgcact gttgtgtcc aaaaatgag atttataag 
      tgtgtttgt tagcgttat tttatttaa cactttcag 
      attgtcaac agcctacaa agctgcagt tttcgaaca 
      ttatgcgct acaagccga tactccaga aaacactat 
      cttgaaaaa tcttgacga atatagaaa tcatgcaga 
      taaagctaa aattcaggc tgcggatat tgctgtctt 
      tccggaata tggtcttac gacagtaat actggacaa 
      tccagagga atatgcagt tgttgtcaa cagcacaaa 
      ccatattat aaacgaact aatgaccat tgccaaaga 
      gcgggccat ttatctagt tgtaaactt actggaaaa 
      agaggagga agccaacaa aaagactaa atattacaa 
      cacaaattt agtcttcga cagagatgg aaaaataat 
      actaaaata ccgcaaaat caatctctt caatgaagg 
      aaaacttac cgcaggccc gaaagatca aacccctac 
      atttaccac agatttcgg tgttacgtt tggcatttt 
      cacatgctt tgatattct tttcgaaaa cccgtcgag 
      aactgtact aaagaatga cgctgttac ggatattgt 
      gttccccac agcttggtt tgcaacaat gcctttctt 
      tacatcttt gagcatcca acatggtta cgctgcagc 
      taacggcgt gaatttact tgctgctaa ctttaataa 
      tccgaaaga aacccatgg tggtagtgg gatctactt 
      aggagatgg tagagttgt ggaaatggt cgtttttga 
      cactccaag cagtaaacc tttaatcca ggaggtacc 
      aaaattatc caaacgaga agacaaaag catctgtcc 
      taaactttc cccatttgg ccttccaaa tgatttaac 
      taaatccaa tgttagcaa ttatcacac agactacga 
      gtttaaagc ttcaaacta caagctcaa gaaaattga 
      tttgaccca aagcaatgt tacaaccac catttgtga 
      ctccacgtt ttgttgcaa ttttgaaat tattattag 
      tacttcaga aacaaacac aagcagtgt ctacaaggt 
      aatggctta tgacggacc tgtttctta cggtaatgt 
      taacgctca tattcgagt gtgtagttt ggtagcttg 
      cgaaagtga ctcagacac gagttgtgg aaaaagagt 
      tgatgttgg tgtcaagtt tacgaaaat tacagttaa 
      aggagattt gataactga tgaaaccta cccaactta 
      ctacacacc tttgactct aaataccta tcttcagcc 
      actaacgca aacgattta ttgtaacaa taagaatag 
      tggaagcaa ctctacttc tgtccaact aaccacaac 
      aaaggcgca aaaggacgt cttagtttt tgggatttt 
      gggaacaac aacagcatc tgcagctgc accttcgcc 
      taattaaat acattgtca tttaattac ttacagaca 
      ataaattat aataagtat t