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Gene information
General information
TC008755 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9558127..9560504
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC008755
      iBeetle screen iB_01407

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection


      Fertility
      fertility after larval injection decreased (no living offspring) -
      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC008755 RA
        MHAIKVLFV LVSLVMGQS VAENGDYLV ELKFVLREE 
        RIPAMIGYI LTNSVELHD IKSEDIVKL SKLDQYDLD 
        ATIRLLNSL NTTAEKAFT VDNLEFILE GFGIEFLEF 
        YRDLQTRLG LDDLTILSI ADDLAIDKL KFLTAIIGS 
        TNVYEILRS GNYDEKHVT EALETAKKT KHQVFAAAR 
        DFMYDKVAS MDAFTFMTI LQTQFGWDR QSSVKLYNA 
        FNLTTRDVY EVEKFRNIF ANVSASLNQ NLSLGVFVA 
        PNKLAVHEW VYNRSLVTV DEISVKVVA NGLEVASVK 
        SQNLGERIV GLETKEAVS DYIDIVQNY HNSSEECYY 
        VTRTGGEIH TYQVEIDEN SFNKTDGLQ FNVGSPLIC 
        DGLLYGLVS EEADEQIMF DIFYTVDNP PPQDYHSKA 
        SLASSILIS LPCAFFIFL LS
      CDS sequence
      • >TC008755 RA
        atgcacgca attaaagtt ttatttgtg ttagtatca 
        ctggttatg ggacagagt gttgccgaa aatggtgat 
        tatttagtg gaactcaag tttgtttta cgcgaagaa 
        cgaatcccg gccatgatc gggtacatt ttaaccaat 
        agtgtcgaa ctgcatgac atcaaaagc gaagatatt 
        gtaaaacta agcaaattg gaccaatac gacttggac 
        gccacgatt cgacttcta aactcttta aataccacg 
        gccgagaag gcatttacc gtggacaac ctagaattc 
        atccttgag ggcttcggt atcgaattt ttggagttt 
        taccgtgat ttgcaaacg cggttgggt cttgacgat 
        ttgacgatt ttgagtatc gctgatgac ttagcaatc 
        gacaagctg aagtttcta acggcaatt attggaagt 
        acgaacgtc tacgagatt ttaagatcg ggaaattat 
        gacgagaaa catgtgact gaagctttg gaaactgcc 
        aaaaagacc aaacatcag gtgttcgca gctgcgagg 
        gactttatg tatgacaaa gtcgcatca atggatgcc 
        ttcactttt atgacaata ttgcaaacc cagtttggc 
        tgggacagg cagagcagt gtcaagtta tacaacgcc 
        tttaacctt acgacacgt gacgtgtac gaagtggaa 
        aaattcagg aacattttt gccaatgtt agcgccagc 
        ttgaaccaa aatctatcg ctgggcgtc tttgtcgca 
        ccaaataag ctggcagtg cacgaatgg gtctacaac 
        aggtctctc gttaccgtt gatgaaatc tcagttaaa 
        gtcgtcgct aacggcctg gaagttgca tcagtgaaa 
        agccaaaat cttggcgaa aggatcgtt ggtctggaa 
        actaaggaa gcagtctct gattatatc gacattgtc 
        cagaattat cacaactcg tccgaggaa tgttactat 
        gttacaaga acaggtggt gaaattcac acatatcaa 
        gtagaaatc gacgaaaat agtttcaat aaaactgac 
        ggtctccag tttaatgta ggaagtccc ctgatttgt 
        gacggcctc ctttacggc cttgttagt gaagaagcc 
        gacgagcaa ataatgttt gacattttc tatactgtt 
        gacaatcct cctccccaa gactatcac agcaaggcc 
        agtctcgca tcctcaatc ttgataagc ctcccatgc 
        gcgtttttt attttcttg ttgtcgtaa
      mRNA sequence
      • >TC008755 RA
        acaattgat tataaaagt ttattgtat tgtattata 
        ttgtattat gagttatca tttgactta gtacctcat 
        gtgatttgg cagaagtca gtgtaagca agaagtgat 
        ttaacacct ccaatcatt gtaacttaa aatgcacgc 
        aattaaagt tttatttgt gttagtatc actggttat 
        gggacagag tgttgccga aaatggtga ttatttagt 
        ggaactcaa gtttgtttt acgcgaaga acgaatccc 
        ggccatgat cgggtacat tttaaccaa tagtgtcga 
        actgcatga catcaaaag cgaagatat tgtaaaact 
        aagcaaatt ggaccaata cgacttgga cgccacgat 
        tcgacttct aaactcttt aaataccac ggccgagaa 
        ggcatttac cgtggacaa cctagaatt catccttga 
        gggcttcgg tatcgaatt tttggagtt ttaccgtga 
        tttgcaaac gcggttggg tcttgacga tttgacgat 
        tttgagtat cgctgatga cttagcaat cgacaagct 
        gaagtttct aacggcaat tattggaag tacgaacgt 
        ctacgagat tttaagatc gggaaatta tgacgagaa 
        acatgtgac tgaagcttt ggaaactgc caaaaagac 
        caaacatca ggtgttcgc agctgcgag ggactttat 
        gtatgacaa agtcgcatc aatggatgc cttcacttt 
        tatgacaat attgcaaac ccagtttgg ctgggacag 
        gcagagcag tgtcaagtt atacaacgc ctttaacct 
        tacgacacg tgacgtgta cgaagtgga aaaattcag 
        gaacatttt tgccaatgt tagcgccag cttgaacca 
        aaatctatc gctgggcgt ctttgtcgc accaaataa 
        gctggcagt gcacgaatg ggtctacaa caggtctct 
        cgttaccgt tgatgaaat ctcagttaa agtcgtcgc 
        taacggcct ggaagttgc atcagtgaa aagccaaaa 
        tcttggcga aaggatcgt tggtctgga aactaagga 
        agcagtctc tgattatat cgacattgt ccagaatta 
        tcacaactc gtccgagga atgttacta tgttacaag 
        aacaggtgg tgaaattca cacatatca agtagaaat 
        cgacgaaaa tagtttcaa taaaactga cggtctcca 
        gtttaatgt aggaagtcc cctgatttg tgacggcct 
        cctttacgg ccttgttag tgaagaagc cgacgagca 
        aataatgtt tgacatttt ctatactgt tgacaatcc 
        tcctcccca agactatca cagcaaggc cagtctcgc 
        atcctcaat cttgataag cctcccatg cgcgttttt 
        tattttctt gttgtcgta attgtatgc gagtcaaat 
        atttgcaaa agattgtga cgcagttct ttaaactaa 
        ttgttagcg cggt
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY