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Gene information
General information
TC008754 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9515677..9517402
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008754
    iBeetle screen iB_01406

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008754 RA
      MGKFSRDRR DIFYRKAKE QGWRARSAF KLLQIDEKF 
      NILEGVTKA VDLCAAPGS WSQVLSRRL YLGEKINIK 
      PKCKLFYTE NEDYMSEDM VECSTSKIV EPKKEEVTE 
      TPQKNKDVK IVAVDLQPM SPLPGVIQL QGDITEYKT 
      AEAIISHFE GDHADLVVC DGAPDVTGL HCIDIYIQA 
      QLLLGALHI TCNVLKPGG TFVAKIFRA KDCDLLTQQ 
      LLMLFEDVI TVKPTSSRN SSIEAFVVC RKFKPPKGF 
      DPMLITPFL DVSNRDFSS LSGVNRVVI PFIVCGDIS 
      AYDSDTTYP LQLEGEEPY QYHPPVQPP IAPPYYFVD 
      DPDKKEKKS KKSQPKASS SRSSDLPDI SQMTLSDKS 
      ESSQSTHKT ESSHSTQLG LAPELKFQD LLQKVTKIR 
      ERKSEKKFV EKVESNETD EETLAFLTG LTQSLNLEQ 
      GTPEYEKLV STLLGPGSS TCTCVHKLN E
    CDS sequence
    • >TC008754 RA
      atgggtaaa ttttcgagg gacaggcgt gatattttt 
      tatcgaaag gccaaagaa caaggctgg cgggcccgt 
      agtgcattt aaacttctc caaatcgat gagaaattc 
      aacattttg gaaggtgta acaaaggcc gtggatcta 
      tgtgcggca ccaggaagt tggagccag gttctgtcc 
      cgtcgctta tatctaggc gagaagatt aatattaag 
      ccaaaatgt aagctgttt tacaccgag aacgaagat 
      tacatgagc gaggatatg gtggaatgc agcacatcg 
      aaaattgtt gagccaaaa aaagaggaa gttacagaa 
      acaccacaa aagaacaaa gacgttaag attgtcgct 
      gttgatttg caaccaatg tcgcctctg cctggggtt 
      atccagctc cagggggac ataacagag tacaagaca 
      gccgaagcc attatcagc cactttgag ggagatcat 
      gcggatttg gttgtttgc gatggggcc ccagatgtc 
      actggactt cattgtatt gatatttac attcaagcc 
      caactttta ttaggtgcg ttgcacatc acttgcaac 
      gttcttaag ccagggggc actttcgtg gctaaaata 
      ttccgggct aaagactgt gatttgctg acccagcag 
      ctgctcatg ctgtttgag gatgtgatc actgtcaaa 
      ccaactagc tcgcgcaat tccagtatc gaagcgttt 
      gttgtttgc cgtaaattc aaaccgccg aaaggtttt 
      gatccgatg ttaataaca ccgtttctt gatgtctca 
      aacagagat ttttcttct ctttctggg gtaaatcgc 
      gtagtaata ccatttata gtttgtggc gatatcagc 
      gcttacgat tccgataca acctatcct ctgcagctt 
      gaaggggag gagccctac cagtatcac ccgccggtg 
      cagccccca atagcccct ccctactat tttgtagat 
      gatccggac aaaaaagaa aaaaaatca aagaaatcg 
      cagccaaag gcaagcagc agtcggagt tctgattta 
      ccagatatt tcccaaatg actttatct gataaaagt 
      gaaagttca caatctaca cataaaacc gaaagttca 
      cactctaca cagttgggt ttggctccg gaactgaag 
      ttccaagac ttgttgcaa aaagttaca aaaattcgt 
      gaaagaaaa tctgaaaaa aagtttgtc gagaaagtg 
      gaatccaat gaaacagat gaggagacg ctggctttt 
      cttacagga ctaacgcaa agtttaaat ctagaacaa 
      gggactcct gaatatgag aaactagtc tcaactctg 
      ctaggtcct ggatcctct acttgtact tgtgtacac 
      aaattaaat gaatga
    mRNA sequence
    • >TC008754 RA
      tgtgtttga caatatcta cataaccta aaaaaaatc 
      aaaagtcaa atagcaaaa actcaaaaa ggaccgttt 
      tcatcaata tgggtaaat tttcgaggg acaggcgtg 
      atatttttt atcgaaagg ccaaagaac aaggctggc 
      gggcccgta gtgcattta aacttctcc aaatcgatg 
      agaaattca acattttgg aaggtgtaa caaaggccg 
      tggatctat gtgcggcac caggaagtt ggagccagg 
      ttctgtccc gtcgcttat atctaggcg agaagatta 
      atattaagc caaaatgta agctgtttt acaccgaga 
      acgaagatt acatgagcg aggatatgg tggaatgca 
      gcacatcga aaattgttg agccaaaaa aagaggaag 
      ttacagaaa caccacaaa agaacaaag acgttaaga 
      ttgtcgctg ttgatttgc aaccaatgt cgcctctgc 
      ctggggtta tccagctcc agggggaca taacagagt 
      acaagacag ccgaagcca ttatcagcc actttgagg 
      gagatcatg cggatttgg ttgtttgcg atggggccc 
      cagatgtca ctggacttc attgtattg atatttaca 
      ttcaagccc aacttttat taggtgcgt tgcacatca 
      cttgcaacg ttcttaagc cagggggca ctttcgtgg 
      ctaaaatat tccgggcta aagactgtg atttgctga 
      cccagcagc tgctcatgc tgtttgagg atgtgatca 
      ctgtcaaac caactagct cgcgcaatt ccagtatcg 
      aagcgtttg ttgtttgcc gtaaattca aaccgccga 
      aaggttttg atccgatgt taataacac cgtttcttg 
      atgtctcaa acagagatt tttcttctc tttctgggg 
      taaatcgcg tagtaatac catttatag tttgtggcg 
      atatcagcg cttacgatt ccgatacaa cctatcctc 
      tgcagcttg aaggggagg agccctacc agtatcacc 
      cgccggtgc agcccccaa tagcccctc cctactatt 
      ttgtagatg atccggaca aaaaagaaa aaaaatcaa 
      agaaatcgc agccaaagg caagcagca gtcggagtt 
      ctgatttac cagatattt cccaaatga ctttatctg 
      ataaaagtg aaagttcac aatctacac ataaaaccg 
      aaagttcac actctacac agttgggtt tggctccgg 
      aactgaagt tccaagact tgttgcaaa aagttacaa 
      aaattcgtg aaagaaaat ctgaaaaaa agtttgtcg 
      agaaagtgg aatccaatg aaacagatg aggagacgc 
      tggcttttc ttacaggac taacgcaaa gtttaaatc 
      tagaacaag ggactcctg aatatgaga aactagtct 
      caactctgc taggtcctg gatcctcta cttgtactt 
      gtgtacaca aattaaatg aatgattta attttttta 
      ttattattt tattgtaat aagtgtgtg aaaatacat 
      tattccaca taaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY