Status
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Gene information
General information
TC033870
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008723,TC008721
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 15007492..15082528
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033870
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01400

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033870 RA
      MIIQRDQED IMETITDEE TLEECNDRT VIHRKSSLA 
      LTPEDEPLD LNRGPQEIK YGFLLPPTR TPPKLLLVF 
      TQHDAVVDT QTKAAEKLG FEVTVVGSE DAALEQFQQ 
      KSHELVIID TRSPKSIDY NTLCRSIRN TRGSQHSAL 
      VAVVRKSMF EKDDVAIMA LLDSGFNRC IVENTNLIW 
      MVNEIIQLK HSDIQPMAQ HATCQSVYA ALDKCKDVV 
      IITDDSYRC QYANRSCEK YLSIKPDEI LGKTLQEQL 
      AYDALQITT MGSSLHRGR EWNGALTLK KKSNEPILT 
      SCRAVPISC AGRLPTHFV MVLDTSHSY DAIGQSRGS 
      LPSVRRGST DVRSVGSDN MRRTSLAKM PLEAPITKL 
      ITLIDQAKD TSPANPQLV QLLERVEEI LRGPELYAP 
      QVKEDRHEE PIVSELISA LLSKQSQPS LALSSRRSS 
      NDSSVYRPS KGGVIKVPA QLKELLDES LSWEFDIFR 
      LEELTKKRP LQYLGMNLF NNFDVAAVL NCDEKVLYN 
      WLIIIEANY HTDNTYHNS THAADVMQA TAGFLEREK 
      LKSIMDPLD EATSLIAAA AHDVDHPGK SNAFLSNSD 
      NPLAILYND ITILESHHA ALMFKLTLG DERVNVFKN 
      LDRETYKIA RHNVIDMIL ATEMTKHFE HLAKFVNVF 
      CTKTSNSDI ELGDQDFPL LTQPENITL IKRMMIKCS 
      DVSNPTRPL RLCVEWARR IAEEYFQQT DEEKERGLP 
      VVMPMFDRN TCSIPKSQI GFVDYIIND MFEAWNAFI 
      DMPDLITYM RQNYIRWKE FDEQGLNTL ADISKLQTS 
      VLMCPMFPL KSSKSN
    • >TC033870 RB
      MCSKGKCLL KCCGGIKNV SSTATNIAN KEVERARKL 
      PSSEAEIRT HHSLIKNPD VLTDQMGDS KTVHVATET 
      REAGTAIDK SLVKRKRTV KRLHSAANV KYMGEAEKI 
      GRSTSRRFT DPEAREFGL RVFSQIAST GNLLNKDEQ 
      KVWDETAEN VAEVLRARG TRLKLDSTS TVNLNDRFV 
      PQIMKAHKK RGPQEIKYG FLLPPTRTP PKLLLVFTQ 
      HDAVVDTQT KAAEKLGFE VTVVGSEDA ALEQFQQKS 
      HELVIIDTR SPKSIDYNT LCRSIRNTR GSQHSALVA 
      VVRKSMFEK DDVAIMALL DSGFNRCIV ENTNLIWMV 
      NEIIQLKHS DIQPMAQHA TCQSVYAAL DKCKDVVII 
      TDDSYRCQY ANRSCEKYL SIKPDEILG KTLQEQLAY 
      DALQITTMG SSLHRGREW NGALTLKKK SNEPILTSC 
      RAVPISCAG RLPTHFVMV LDTSHSYDA IGQSRGSLP 
      SVRRGSTDV RSVGSDNMR RTSLAKMPL EAPITKLIT 
      LIDQAKDTS PANPQLVQL LERVEEILR GPELYAPQV 
      KEDRHEEPI VSELISALL SKQSQPSLA LSSRRSSND 
      SSVYRPSKG GVIKVPAQL KELLDESLS WEFDIFRLE 
      ELTKKRPLQ YLGMNLFNN FDVAAVLNC DEKVLYNWL 
      IIIEANYHT DNTYHNSTH AADVMQATA GFLEREKLK 
      SIMDPLDEA TSLIAAAAH DVDHPGKSN AFLSNSDNP 
      LAILYNDIT ILESHHAAL MFKLTLGDE RVNVFKNLD 
      RETYKIARH NVIDMILAT EMTKHFEHL AKFVNVFCT 
      KTSNSDIEL GDQDFPLLT QPENITLIK RMMIKCSDV 
      SNPTRPLRL CVEWARRIA EEYFQQTDE EKERGLPVV 
      MPMFDRNTC SIPKSQIGF VDYIINDMF EAWNAFIDM 
      PDLITYMRQ NYIRWKEFD EQGLNTLAD ISKLQTSVL 
      MCPMFPLKS SKSN
    CDS sequence
    • >TC033870 RA
      atgattatt cagagggat caggaggat atcatggag 
      actatcact gacgaggag acattggag gaatgcaac 
      gataggaca gtcattcac cgaaagagc tctttggcg 
      ctgacacca gaggacgaa cccttggat ctcaacaga 
      ggaccgcag gaaatcaaa tacggtttt ttgttgccg 
      ccgaccaga acaccacca aagttgctg ttggttttt 
      acccaacac gatgccgtt gttgacact caaacaaaa 
      gcggcggaa aaactcgga ttcgaagtg actgtcgtc 
      ggttctgaa gacgctgca ttagaacaa ttccaacaa 
      aaatcacac gaacttgtg attatagac acacgctcg 
      ccgaaatca atcgattac aacacatta tgtcgatca 
      atcagaaac acgagggga agtcaacat tcggctttg 
      gtagctgtc gtaagaaaa agcatgttc gaaaaagat 
      gacgtggct attatggcg ctactggat tcgggattt 
      aataggtgc attgtagag aacacgaat ttaatctgg 
      atggtcaac gagatcatc caactgaaa cacagtgat 
      atccagcct atggctcaa cacgcgacc tgtcagtcc 
      gtgtatgca gctttggac aaatgcaaa gacgtggtt 
      attataacc gacgatagt tatcgatgt cagtacgcg 
      aacagatct tgcgaaaaa tatctgagt ataaaaccc 
      gacgagatt ttgggtaag acgcttcaa gagcaatta 
      gcttacgat gctctccaa ataacaaca atgggctcg 
      tcgttacac agaggtcga gaatggaac ggggctctg 
      acgttgaag aaaaaaagc aacgaaccg attttgacc 
      tcgtgtcgg gctgtgccc atttcgtgt gccggaaga 
      ctaccaaca cattttgtg atggttttg gacacttcg 
      cattcctat gacgcgata ggacaatca cgaggaagt 
      cttccctcg gtcagaagg gggtcgacg gatgtaagg 
      agtgtcggg agtgacaac atgaggagg acttccttg 
      gcgaaaatg cctctggag gctcccatc accaagtta 
      attactctg atcgatcaa gcgaaagac acatctccg 
      gcgaaccct cagctggtg caattactc gaaagggtg 
      gaagaaatt ctgcgaggt cccgaatta tacgcgccc 
      caagtgaaa gaggaccgc cacgaggag cccatcgta 
      tcggagctg atctcggct ttgctctcc aaacaatct 
      cagccttcg ctcgccctg tcttcgcga agaagcagt 
      aacgattcg tcggtgtat cgaccatcg aaaggtggc 
      gtcatcaaa gtcccagca cagctgaag gagctcctc 
      gacgagtcc ctatcgtgg gaatttgac atttttcgt 
      ttggaagag ttgacgaaa aaacgaccg cttcaatat 
      ttaggaatg aatcttttt aataacttc gacgtggct 
      gcggtcctc aattgtgac gaaaaagtt ctctataat 
      tggctcatt attatcgag gccaattat cataccgat 
      aatacctat cacaactcg acacatgcc gccgacgta 
      atgcaggca acggcaggc tttctggaa agagagaag 
      ctgaaatcg atcatggac ccactcgac gaggcaaca 
      agtctaata gcagccgct gctcacgac gtcgaccat 
      ccaggcaaa tccaacgca ttcttgtcg aactcggac 
      aatccatta gctatatta tataacgac ataaccatt 
      ttagaatcg caccatgca gccctcatg ttcaaatta 
      actttaggt gacgagaga gtaaacgtt tttaaaaat 
      ctagacagg gagacgtac aagatcgcc cgacacaac 
      gtgatcgac atgattcta gcaactgaa atgacaaaa 
      cattttgag catctggca aagttcgtt aatgttttc 
      tgcacgaaa acgtccaat agtgatatc gaactgggc 
      gatcaggac tttccgctc ctgacgcaa ccggagaac 
      atcaccctg atcaagcgg atgatgata aagtgttcc 
      gacgtttcg aatccgacg agacctttg agattgtgc 
      gtcgaatgg gcgaggagg atcgctgag gagtatttc 
      cagcagacg gacgaggag aaagaacgg ggtctgccc 
      gtggtcatg ccgatgttc gacaggaac acttgctcc 
      attcccaaa tcgcaaatt ggatttgtc gattatatc 
      atcaatgac atgtttgaa gcttggaat gctttcatc 
      gatatgccc gatttaata acgtacatg agacagaat 
      tacatcagg tggaaggag ttcgacgaa caaggtctg 
      aatacctta gctgatatt agtaaactt caaactagc 
      gtacttatg tgtccgatg tttccgctg aaatcttca 
      aaatccaac tga
    • >TC033870 RB
      atgtgttca aaaggaaag tgtttgttg aagtgctgc 
      gggggcata aagaacgta tcatcaaca gcaaccaac 
      attgcaaat aaagaggta gagcgtgct cgaaaactg 
      ccatcctcc gaagccgag attcgcact catcactct 
      ttgataaag aatccagac gtactgaca gatcaaatg 
      ggtgactcc aaaacggtt catgtcgct accgaaacc 
      agagaagcc ggaactgca attgacaag agtctggtc 
      aaacgaaaa cgcaccgta aaacggctt cattccgct 
      gctaatgta aaatatatg ggagaagcg gagaaaata 
      ggaaggtcg acgagtcgg agattcacc gatccggag 
      gctagagag ttcggtttg agggtgttt tcgcagatt 
      gcgtccact ggaaatctg ctaaataag gacgagcag 
      aaagtttgg gacgaaact gccgaaaat gtcgccgaa 
      gttttgaga gcgagagga actagactc aagcttgat 
      tcgacaagc accgtgaac ttgaatgac agatttgta 
      ccgcaaatt atgaaagca cataagaag agaggaccg 
      caggaaatc aaatacggt tttttgttg ccgccgacc 
      agaacacca ccaaagttg ctgttggtt tttacccaa 
      cacgatgcc gttgttgac actcaaaca aaagcggcg 
      gaaaaactc ggattcgaa gtgactgtc gtcggttct 
      gaagacgct gcattagaa caattccaa caaaaatca 
      cacgaactt gtgattata gacacacgc tcgccgaaa 
      tcaatcgat tacaacaca ttatgtcga tcaatcaga 
      aacacgagg ggaagtcaa cattcggct ttggtagct 
      gtcgtaaga aaaagcatg ttcgaaaaa gatgacgtg 
      gctattatg gcgctactg gattcggga tttaatagg 
      tgcattgta gagaacacg aatttaatc tggatggtc 
      aacgagatc atccaactg aaacacagt gatatccag 
      cctatggct caacacgcg acctgtcag tccgtgtat 
      gcagctttg gacaaatgc aaagacgtg gttattata 
      accgacgat agttatcga tgtcagtac gcgaacaga 
      tcttgcgaa aaatatctg agtataaaa cccgacgag 
      attttgggt aagacgctt caagagcaa ttagcttac 
      gatgctctc caaataaca acaatgggc tcgtcgtta 
      cacagaggt cgagaatgg aacggggct ctgacgttg 
      aagaaaaaa agcaacgaa ccgattttg acctcgtgt 
      cgggctgtg cccatttcg tgtgccgga agactacca 
      acacatttt gtgatggtt ttggacact tcgcattcc 
      tatgacgcg ataggacaa tcacgagga agtcttccc 
      tcggtcaga agggggtcg acggatgta aggagtgtc 
      gggagtgac aacatgagg aggacttcc ttggcgaaa 
      atgcctctg gaggctccc atcaccaag ttaattact 
      ctgatcgat caagcgaaa gacacatct ccggcgaac 
      cctcagctg gtgcaatta ctcgaaagg gtggaagaa 
      attctgcga ggtcccgaa ttatacgcg ccccaagtg 
      aaagaggac cgccacgag gagcccatc gtatcggag 
      ctgatctcg gctttgctc tccaaacaa tctcagcct 
      tcgctcgcc ctgtcttcg cgaagaagc agtaacgat 
      tcgtcggtg tatcgacca tcgaaaggt ggcgtcatc 
      aaagtccca gcacagctg aaggagctc ctcgacgag 
      tccctatcg tgggaattt gacattttt cgtttggaa 
      gagttgacg aaaaaacga ccgcttcaa tatttagga 
      atgaatctt tttaataac ttcgacgtg gctgcggtc 
      ctcaattgt gacgaaaaa gttctctat aattggctc 
      attattatc gaggccaat tatcatacc gataatacc 
      tatcacaac tcgacacat gccgccgac gtaatgcag 
      gcaacggca ggctttctg gaaagagag aagctgaaa 
      tcgatcatg gacccactc gacgaggca acaagtcta 
      atagcagcc gctgctcac gacgtcgac catccaggc 
      aaatccaac gcattcttg tcgaactcg gacaatcca 
      ttagctata ttatataac gacataacc attttagaa 
      tcgcaccat gcagccctc atgttcaaa ttaacttta 
      ggtgacgag agagtaaac gtttttaaa aatctagac 
      agggagacg tacaagatc gcccgacac aacgtgatc 
      gacatgatt ctagcaact gaaatgaca aaacatttt 
      gagcatctg gcaaagttc gttaatgtt ttctgcacg 
      aaaacgtcc aatagtgat atcgaactg ggcgatcag 
      gactttccg ctcctgacg caaccggag aacatcacc 
      ctgatcaag cggatgatg ataaagtgt tccgacgtt 
      tcgaatccg acgagacct ttgagattg tgcgtcgaa 
      tgggcgagg aggatcgct gaggagtat ttccagcag 
      acggacgag gagaaagaa cggggtctg cccgtggtc 
      atgccgatg ttcgacagg aacacttgc tccattccc 
      aaatcgcaa attggattt gtcgattat atcatcaat 
      gacatgttt gaagcttgg aatgctttc atcgatatg 
      cccgattta ataacgtac atgagacag aattacatc 
      aggtggaag gagttcgac gaacaaggt ctgaatacc 
      ttagctgat attagtaaa cttcaaact agcgtactt 
      atgtgtccg atgtttccg ctgaaatct tcaaaatcc 
      aactga
    mRNA sequence
    • >TC033870 RA
      agtgtacac ataaacctc ctagaacag gctcgtcgt 
      cgtcgtgag tggcttgag caccagatt gccacacac 
      caaactttc tcagtgcga ctccgacgg tgtgccagg 
      tgcctgctg gtcctgctg tgccttccg atgccgctt 
      cctacccag ctcaggagg agacaaccg tatcttatc 
      tgctgattt agtgcacca acgcgtgca gtgcagtga 
      tgcgacgag ttttagagc tgtgatttg tgcaatgtg 
      atcgatttt tcgttgaaa ttttttgtg actggacta 
      atgaggctg cctacaccc acgcttcgt gagtttgtc 
      aattatcaa aggacccaa gtcataaca gtgggcgat 
      aatggggga gtttggtaa ttgatcaaa ttcttgagg 
      tgtcacata aaatgagaa aaaattgtg ttcgccgta 
      gtatttgca aacaaaaac tcattgttc ggataataa 
      atcaataac aaattacgc acagaacaa ttagattca 
      aaaacgata ttaaaattt ataaagggt cgcattggc 
      taaaactta tcgcactag atgagaata tttacgaac 
      tcagcttgc attgttggc acattttac tcgacgcaa 
      actttaatc gaagatttc aactaaaac ctgtcattt 
      tataattcc gctataaga gcgcaataa ataagtgat 
      gattcaatg ccatcttca gatttttat gccattttt 
      aaaatgtca agaataacc attctttat catcgtaat 
      atgtcaatg ctgttgcat tgattcctc gttaaataa 
      aatgccaga ttagttatt ttagctatt tctatatta 
      ttattgcca ttttcgatc tactttcat tagcgctgt 
      aatgaaggc tggctccaa catttttcc gctggaata 
      tgtggaatg tagaattct ttgaaagaa agtcagttt 
      tttgttcca ttttgaaat cacgggttt gaaatgcac 
      ttcatgaaa tacctgtac gaatgttta cttttccgc 
      ctacatttc ggtttgatg ttgaagctc tcaggttat 
      gggtcgtcg ttaaaatgc caaaatagt gcttccgta 
      aatatttaa gtgataaaa caatgttta gttgatagt 
      gcacgttta ataaaaaca gtgttgtga tgatcggat 
      taaacgtgg attagttgt tggaaatat gattattca 
      gagggatca ggaggatat catggagac tatcactga 
      cgaggagac attggagga atgcaacga taggacagt 
      cattcaccg aaagagctc tttggcgct gacaccaga 
      ggacgaacc cttggatct caacagagg accgcagga 
      aatcaaata cggtttttt gttgccgcc gaccagaac 
      accaccaaa gttgctgtt ggtttttac ccaacacga 
      tgccgttgt tgacactca aacaaaagc ggcggaaaa 
      actcggatt cgaagtgac tgtcgtcgg ttctgaaga 
      cgctgcatt agaacaatt ccaacaaaa atcacacga 
      acttgtgat tatagacac acgctcgcc gaaatcaat 
      cgattacaa cacattatg tcgatcaat cagaaacac 
      gaggggaag tcaacattc ggctttggt agctgtcgt 
      aagaaaaag catgttcga aaaagatga cgtggctat 
      tatggcgct actggattc gggatttaa taggtgcat 
      tgtagagaa cacgaattt aatctggat ggtcaacga 
      gatcatcca actgaaaca cagtgatat ccagcctat 
      ggctcaaca cgcgacctg tcagtccgt gtatgcagc 
      tttggacaa atgcaaaga cgtggttat tataaccga 
      cgatagtta tcgatgtca gtacgcgaa cagatcttg 
      cgaaaaata tctgagtat aaaacccga cgagatttt 
      gggtaagac gcttcaaga gcaattagc ttacgatgc 
      tctccaaat aacaacaat gggctcgtc gttacacag 
      aggtcgaga atggaacgg ggctctgac gttgaagaa 
      aaaaagcaa cgaaccgat tttgacctc gtgtcgggc 
      tgtgcccat ttcgtgtgc cggaagact accaacaca 
      ttttgtgat ggttttgga cacttcgca ttcctatga 
      cgcgatagg acaatcacg aggaagtct tccctcggt 
      cagaagggg gtcgacgga tgtaaggag tgtcgggag 
      tgacaacat gaggaggac ttccttggc gaaaatgcc 
      tctggaggc tcccatcac caagttaat tactctgat 
      cgatcaagc gaaagacac atctccggc gaaccctca 
      gctggtgca attactcga aagggtgga agaaattct 
      gcgaggtcc cgaattata cgcgcccca agtgaaaga 
      ggaccgcca cgaggagcc catcgtatc ggagctgat 
      ctcggcttt gctctccaa acaatctca gccttcgct 
      cgccctgtc ttcgcgaag aagcagtaa cgattcgtc 
      ggtgtatcg accatcgaa aggtggcgt catcaaagt 
      cccagcaca gctgaagga gctcctcga cgagtccct 
      atcgtggga atttgacat ttttcgttt ggaagagtt 
      gacgaaaaa acgaccgct tcaatattt aggaatgaa 
      tctttttaa taacttcga cgtggctgc ggtcctcaa 
      ttgtgacga aaaagttct ctataattg gctcattat 
      tatcgaggc caattatca taccgataa tacctatca 
      caactcgac acatgccgc cgacgtaat gcaggcaac 
      ggcaggctt tctggaaag agagaagct gaaatcgat 
      catggaccc actcgacga ggcaacaag tctaatagc 
      agccgctgc tcacgacgt cgaccatcc aggcaaatc 
      caacgcatt cttgtcgaa ctcggacaa tccattagc 
      tatattata taacgacat aaccatttt agaatcgca 
      ccatgcagc cctcatgtt caaattaac tttaggtga 
      cgagagagt aaacgtttt taaaaatct agacaggga 
      gacgtacaa gatcgcccg acacaacgt gatcgacat 
      gattctagc aactgaaat gacaaaaca ttttgagca 
      tctggcaaa gttcgttaa tgttttctg cacgaaaac 
      gtccaatag tgatatcga actgggcga tcaggactt 
      tccgctcct gacgcaacc ggagaacat caccctgat 
      caagcggat gatgataaa gtgttccga cgtttcgaa 
      tccgacgag acctttgag attgtgcgt cgaatgggc 
      gaggaggat cgctgagga gtatttcca gcagacgga 
      cgaggagaa agaacgggg tctgcccgt ggtcatgcc 
      gatgttcga caggaacac ttgctccat tcccaaatc 
      gcaaattgg atttgtcga ttatatcat caatgacat 
      gtttgaagc ttggaatgc tttcatcga tatgcccga 
      tttaataac gtacatgag acagaatta catcaggtg 
      gaaggagtt cgacgaaca aggtctgaa taccttagc 
      tgatattag taaacttca aactagcgt acttatgtg 
      tccgatgtt tccgctgaa atcttcaaa atccaactg 
      atgcaaact gtttcgtaa gaataataa taaaagata 
      aggtagtgg ttatgctaa tttggtatt tttaggtca 
      cagtgttga cgccgataa attgaagaa attgttgcg 
      aaaaaaact gtgaatgtg aactaacaa aatgtgcat 
      atcgtgtta tttatatat ctatatatt atacagttt 
      atactataa ataaaagta ttgcgtacc tcattattt 
      aacgtgcat gctggacgt gcattgaat gaaatattt 
      tattaaatt attatttgt tttaatttt aatatgttt 
      ttagttagg atgtgcaat gatgttctt actgttttc 
      tattgacac tgtattgat gtgcaatat aagatttat 
      aaaaaa
    • >TC033870 RB
      tcggttaag ctttggtgg cggcgcacc ccgttgaaa 
      tgcctgtgg tctataaat gtgttcaaa aggaaagtg 
      tttgttgaa gtgctgcgg gggcataaa gaacgtatc 
      atcaacagc aaccaacat tgcaaataa agaggtaga 
      gcgtgctcg aaaactgcc atcctccga agccgagat 
      tcgcactca tcactcttt gataaagaa tccagacgt 
      actgacaga tcaaatggg tgactccaa aacggttca 
      tgtcgctac cgaaaccag agaagccgg aactgcaat 
      tgacaagag tctggtcaa acgaaaacg caccgtaaa 
      acggcttca ttccgctgc taatgtaaa atatatggg 
      agaagcgga gaaaatagg aaggtcgac gagtcggag 
      attcaccga tccggaggc tagagagtt cggtttgag 
      ggtgttttc gcagattgc gtccactgg aaatctgct 
      aaataagga cgagcagaa agtttggga cgaaactgc 
      cgaaaatgt cgccgaagt tttgagagc gagaggaac 
      tagactcaa gcttgattc gacaagcac cgtgaactt 
      gaatgacag atttgtacc gcaaattat gaaagcaca 
      taagaagag aggaccgca ggaaatcaa atacggttt 
      tttgttgcc gccgaccag aacaccacc aaagttgct 
      gttggtttt tacccaaca cgatgccgt tgttgacac 
      tcaaacaaa agcggcgga aaaactcgg attcgaagt 
      gactgtcgt cggttctga agacgctgc attagaaca 
      attccaaca aaaatcaca cgaacttgt gattataga 
      cacacgctc gccgaaatc aatcgatta caacacatt 
      atgtcgatc aatcagaaa cacgagggg aagtcaaca 
      ttcggcttt ggtagctgt cgtaagaaa aagcatgtt 
      cgaaaaaga tgacgtggc tattatggc gctactgga 
      ttcgggatt taataggtg cattgtaga gaacacgaa 
      tttaatctg gatggtcaa cgagatcat ccaactgaa 
      acacagtga tatccagcc tatggctca acacgcgac 
      ctgtcagtc cgtgtatgc agctttgga caaatgcaa 
      agacgtggt tattataac cgacgatag ttatcgatg 
      tcagtacgc gaacagatc ttgcgaaaa atatctgag 
      tataaaacc cgacgagat tttgggtaa gacgcttca 
      agagcaatt agcttacga tgctctcca aataacaac 
      aatgggctc gtcgttaca cagaggtcg agaatggaa 
      cggggctct gacgttgaa gaaaaaaag caacgaacc 
      gattttgac ctcgtgtcg ggctgtgcc catttcgtg 
      tgccggaag actaccaac acattttgt gatggtttt 
      ggacacttc gcattccta tgacgcgat aggacaatc 
      acgaggaag tcttccctc ggtcagaag ggggtcgac 
      ggatgtaag gagtgtcgg gagtgacaa catgaggag 
      gacttcctt ggcgaaaat gcctctgga ggctcccat 
      caccaagtt aattactct gatcgatca agcgaaaga 
      cacatctcc ggcgaaccc tcagctggt gcaattact 
      cgaaagggt ggaagaaat tctgcgagg tcccgaatt 
      atacgcgcc ccaagtgaa agaggaccg ccacgagga 
      gcccatcgt atcggagct gatctcggc tttgctctc 
      caaacaatc tcagccttc gctcgccct gtcttcgcg 
      aagaagcag taacgattc gtcggtgta tcgaccatc 
      gaaaggtgg cgtcatcaa agtcccagc acagctgaa 
      ggagctcct cgacgagtc cctatcgtg ggaatttga 
      catttttcg tttggaaga gttgacgaa aaaacgacc 
      gcttcaata tttaggaat gaatctttt taataactt 
      cgacgtggc tgcggtcct caattgtga cgaaaaagt 
      tctctataa ttggctcat tattatcga ggccaatta 
      tcataccga taataccta tcacaactc gacacatgc 
      cgccgacgt aatgcaggc aacggcagg ctttctgga 
      aagagagaa gctgaaatc gatcatgga cccactcga 
      cgaggcaac aagtctaat agcagccgc tgctcacga 
      cgtcgacca tccaggcaa atccaacgc attcttgtc 
      gaactcgga caatccatt agctatatt atataacga 
      cataaccat tttagaatc gcaccatgc agccctcat 
      gttcaaatt aactttagg tgacgagag agtaaacgt 
      ttttaaaaa tctagacag ggagacgta caagatcgc 
      ccgacacaa cgtgatcga catgattct agcaactga 
      aatgacaaa acattttga gcatctggc aaagttcgt 
      taatgtttt ctgcacgaa aacgtccaa tagtgatat 
      cgaactggg cgatcagga ctttccgct cctgacgca 
      accggagaa catcaccct gatcaagcg gatgatgat 
      aaagtgttc cgacgtttc gaatccgac gagaccttt 
      gagattgtg cgtcgaatg ggcgaggag gatcgctga 
      ggagtattt ccagcagac ggacgagga gaaagaacg 
      gggtctgcc cgtggtcat gccgatgtt cgacaggaa 
      cacttgctc cattcccaa atcgcaaat tggatttgt 
      cgattatat catcaatga catgtttga agcttggaa 
      tgctttcat cgatatgcc cgatttaat aacgtacat 
      gagacagaa ttacatcag gtggaagga gttcgacga 
      acaaggtct gaatacctt agctgatat tagtaaact 
      tcaaactag cgtacttat gtgtccgat gtttccgct 
      gaaatcttc aaaatccaa ctgatgcaa actgtttcg 
      taagaataa taataaaag ataaggtag tggttatgc 
      taatttggt atttttagg tcacagtgt tgacgccga 
      taaattgaa gaaattgtt gcgaaaaaa actgtgaat 
      gtgaactaa caaaatgtg catatcgtg ttatttata 
      tatctatat attatacag tttatacta taaataaaa 
      gtattgcgt acctcatta tttaacgtg catgctgga 
      cgtgcattg aatgaaata ttttattaa attattatt 
      tgttttaat tttaatatg tttttagtt aggatgtgc 
      aatgatgtt cttactgtt ttctattga cactgtatt 
      gatgtgcaa tataagatt tataaaaaa
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