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Gene information
General information
TC008714 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 14780516..14788344
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008714
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01397

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva completeeverted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva cuticle dorsal irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head appendages sometimes randomnot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head appendages sometimesshape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head appendages sometimesposition irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    legs sometimes randomnot present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008714 RA
      MKIAIIGAG AAGLCAGRH CLRENIAFD IFEQTGNLG 
      GTWNYTDLV GCDENGVPI HSSMYKGLR TNLPKELMA 
      FEDFPYPKQ NRSYLLQDE VLDYVRSYS DKFHINPHI 
      KYFKRVIRI ERQNFLWSV HYEDVKNKQ KDMEHYDAV 
      IICNGHYSD PFIPDVPGI ESFSGRVKH SHDYRTPEP 
      YANKKVLIL GSGPSGLDI SQQISNVAT KVFLSHRSK 
      DPLPVPDIL HQKCLIKEF VENKAIFED GTSEEIDDV 
      VFCTGYNYN FPFLSTNCG VKITDNYVH PLYKQIISI 
      ENPTLAFLG IPFKVCPFP LFDIQVRFF LATLTGHFK 
      LPKKEDMLQ ELVEEEKRK SGLPRPKYH ELGKAQGSY 
      FNDLSETAK IKMVPQVVQ KLYVRVMMN RNRNNCFEI 
      IDDDNFVQI
    CDS sequence
    • >TC008714 RA
      atgaaaatt gctattatt ggtgccggg gcggctggt 
      ttgtgtgcc gggagacac tgcttgcga gaaaatatc 
      gcatttgac atttttgaa caaactggg aatttgggg 
      ggaacatgg aactacaca gatctagta ggttgtgac 
      gaaaacggc gtaccaatt cattcctct atgtacaaa 
      ggtctaaga acgaacttg ccgaaagaa ctgatggcc 
      tttgaggat ttcccatat cccaaacaa aatcgttct 
      tatttacta caagacgaa gttctagat tacgttaga 
      agttattcg gataaattc cacatcaat cctcatatc 
      aaatacttt aaacgcgtg atcaggatt gagcgtcaa 
      aatttttta tggtctgtt cattacgaa gatgttaaa 
      aataaacag aaagatatg gaacattac gacgctgtt 
      attatttgt aatggtcac tacagcgat cccttcatt 
      cccgatgtt ccaggaatt gagagtttt tcaggaaga 
      gttaaacac agtcacgat tacagaaca cctgaacct 
      tatgcaaat aaaaaggtg ttgattctt ggttcaggt 
      ccttcagga ctagacatt tcgcaacag atttccaac 
      gtagccacg aaggtattc ctgagtcat agatcgaaa 
      gatcctcta ccagttcca gatattttg catcagaaa 
      tgtttaatt aaagaattt gtcgaaaac aaggcaatt 
      ttcgaagac ggcacgagc gaagaaatt gatgatgtt 
      gttttctgc actggttac aattacaat tttcctttt 
      ttatccact aactgcgga gtcaaaatc accgataac 
      tacgttcac ccgttgtat aaacaaatt attagtata 
      gaaaatccc acgttggct tttcttggt attccattc 
      aaagtatgt ccttttcca ctatttgat atacaagtc 
      agattcttt ttggccaca cttactggt cattttaag 
      ctaccgaag aaagaagat atgttacaa gagttagtt 
      gaggaagaa aaacggaag agtggttta cctagacca 
      aaataccat gaactgggc aaagcccaa ggaagttat 
      tttaatgac ttgtccgaa acagcaaag atcaaaatg 
      gtacctcaa gtggtacaa aagttgtat gtgagggtt 
      atgatgaac cgaaatcga aacaactgt tttgagatt 
      attgatgat gacaatttc gtgcaaata taa
    mRNA sequence
    • >TC008714 RA
      gtgttgtta cagacgtgt tgtctttga ctatttttg 
      ttttaaatg ttttaactg atatacttt aattttgtt 
      aaattgtta tatcaagac aaaactcag taacattat 
      tattactgg taaggtgac gttactaaa gttcttttt 
      gacctattt atgactttc accttgaat gtgggtaat 
      tttatcttc attttttag atataatat gaggagttg 
      tatttgaaa ttgcagttt ttggcacta cgtcacaaa 
      atcagaaag agatgacaa tgcgtattt taactattt 
      tttagttta caaaaactc atttagatc gaaaatttt 
      ccatttctg ctacaatta tctcgactg tgtaagttt 
      tcaaaaact gtattttga attcatcac atcatagac 
      aagtacaat gaaaattgc tattattgg tgccggggc 
      ggctggttt gtgtgccgg gagacactg cttgcgaga 
      aaatatcgc atttgacat ttttgaaca aactgggaa 
      tttgggggg aacatggaa ctacacaga tctagtagg 
      ttgtgacga aaacggcgt accaattca ttcctctat 
      gtacaaagg tctaagaac gaacttgcc gaaagaact 
      gatggcctt tgaggattt cccatatcc caaacaaaa 
      tcgttctta tttactaca agacgaagt tctagatta 
      cgttagaag ttattcgga taaattcca catcaatcc 
      tcatatcaa atactttaa acgcgtgat caggattga 
      gcgtcaaaa ttttttatg gtctgttca ttacgaaga 
      tgttaaaaa taaacagaa agatatgga acattacga 
      cgctgttat tatttgtaa tggtcacta cagcgatcc 
      cttcattcc cgatgttcc aggaattga gagtttttc 
      aggaagagt taaacacag tcacgatta cagaacacc 
      tgaacctta tgcaaataa aaaggtgtt gattcttgg 
      ttcaggtcc ttcaggact agacatttc gcaacagat 
      ttccaacgt agccacgaa ggtattcct gagtcatag 
      atcgaaaga tcctctacc agttccaga tattttgca 
      tcagaaatg tttaattaa agaatttgt cgaaaacaa 
      ggcaatttt cgaagacgg cacgagcga agaaattga 
      tgatgttgt tttctgcac tggttacaa ttacaattt 
      tcctttttt atccactaa ctgcggagt caaaatcac 
      cgataacta cgttcaccc gttgtataa acaaattat 
      tagtataga aaatcccac gttggcttt tcttggtat 
      tccattcaa agtatgtcc ttttccact atttgatat 
      acaagtcag attcttttt ggccacact tactggtca 
      ttttaagct accgaagaa agaagatat gttacaaga 
      gttagttga ggaagaaaa acggaagag tggtttacc 
      tagaccaaa ataccatga actgggcaa agcccaagg 
      aagttattt taatgactt gtccgaaac agcaaagat 
      caaaatggt acctcaagt ggtacaaaa gttgtatgt 
      gagggttat gatgaaccg aaatcgaaa caactgttt 
      tgagattat tgatgatga caatttcgt gcaaatata 
      atgttaatg tgactttgt aattgtgtt ttgtgcata 
      atattaatt gaaaataaa ttttatttg cactt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY