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Gene information
General information
TC033864
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008710
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 14526403..14533977
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033864
    iBeetle screen iB_01395

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    metathorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen constricted - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033864 RA
      MRTLVSLMW VRQLVGKQV VVVTIQYRL GSLGFLSTG 
      TKTLPGNAG LWDMVLAVT WTRNYIGFF GGNPNNIVV 
      MGHGTGASS AIMVALSNV AKGLASGIV AMSGSSLSQ 
      FATDDAPAN TAMDVAEAN GCPVGSELT MVRCLQNLS 
      PAAIIKVDS VIEATRLQN RGFVSGLAG KLGSAPVFE 
      GRRDGRSLP PVAEVEPVN DPESTQKKI PLLTGITKD 
      ETKRACHGQ FRDEITNKL QKVPEFLDK VLVKNLQNA 
      IGINRDKNS TSGKLLNLL DPNQFKNYL QYKRNNLHE 
      GLAKIAEAT ADALFNAPA FLTADAWSK HGAPAFLYR 
      FEHVGKRRK GYNFLKGLP LVGNHSQSD DVSNDTVAH 
      GDELSYIFD AQDMEGKSL ESDTGNEED NKVRDIFTQ 
      MISDFARHG KLSVEGKHV KPFSITDNN FVQISPKPK 
      VSNGFRYCE MGLWLGLAQ RLQSSTCEL FKVLDTQLK 
      NIDLGGGTK KIGETITGI VKGGGSKKP PDVISKSAG 
      LLSGNKDKK VFGIGG
    CDS sequence
    • >TC033864 RA
      atgaggact ctcgttagt ctcatgtgg gttcggcaa 
      ttggtcggt aagcaggtc gtggttgtg accatccaa 
      taccgctta ggctccttg ggtttccta agtactgga 
      accaagacc cttcctgga aacgccggg ctatgggat 
      atggtcttg gcggttact tggactcga aattacatt 
      ggatttttc ggtggtaac cccaacaac attgtggta 
      atgggtcat ggtaccggt gctagtagc gccatcatg 
      gtcgcttta tctaacgtt gcgaagggc ttagccagc 
      ggaattgta gcaatgtct gggagttca ctgtcccaa 
      tttgccact gacgacgct cctgccaac actgccatg 
      gatgtggcc gaagccaat gggtgtcca gttgggtcc 
      gaactgacc atggttcgg tgtctgcag aatttgtct 
      cctgctgca ataataaaa gtcgattct gttattgaa 
      gccacgcga ttacaaaat cgcggtttt gtttccggt 
      cttgctgga aaacttgga tccgctcca gtgtttgaa 
      ggtcgaagg gatggtcgt tcgttgcca cccgttgca 
      gaggttgaa cctgtgaac gatccagaa agtacccaa 
      aagaaaata ccgctattg accgggata acgaaagat 
      gagactaag agggcttgc catgggcaa ttcagggat 
      gaaattacc aataaattg caaaaagtt cccgaattt 
      ttggataag gtacttgtc aagaacctt cagaacgca 
      attgggata aatcgggac aagaattca acttctggc 
      aagttgcta aatcttttg gatcccaac caatttaaa 
      aactatctt caatataag agaaataat cttcatgag 
      ggtcttgcc aaaatagcg gaagctaca gcggatgcg 
      ttattcaac gctccagct ttcctgacc gctgatgct 
      tggtccaaa catggtgca ccggcattt ttgtaccgg 
      tttgaacac gttgggaaa cgaaggaaa gggtacaat 
      tttttgaaa ggattacca cttgtcgga aatcattct 
      cagagtgat gacgtttcc aacgacaca gttgcccac 
      ggcgatgag ctttcttac atcttcgac gctcaagat 
      atggaaggc aagtcgtta gaatcggac acaggcaac 
      gaagaggac aacaaagtc cgtgatatc ttcactcag 
      atgatttcg gattttgct cgtcatgga aaactgagt 
      gtcgaaggc aaacacgtg aaacccttt tccatcacc 
      gacaacaat tttgtgcaa atttcgcct aaaccaaaa 
      gtgtcgaat ggttttaga tactgcgaa atggggctt 
      tggttgggc ttggctcaa agattgcaa agtagcact 
      tgtgaactc ttcaaagtt ttggataca caactgaag 
      aacattgac ttgggcggt ggtacgaaa aaaattgga 
      gagaccatt acgggcatt gtcaaagga ggaggaagc 
      aagaaacca ccagatgta atctcaaaa tcagcaggc 
      cttttaagt gggaataaa gacaaaaag gtgtttgga 
      attggggga tga
    mRNA sequence
    • >TC033864 RA
      ggaatctaa tgtaacctc atgaggact ctcgttagt 
      ctcatgtgg gttcggcaa ttggtcggt aagcaggtc 
      gtggttgtg accatccaa taccgctta ggctccttg 
      ggtttccta agtactgga accaagacc cttcctgga 
      aacgccggg ctatgggat atggtcttg gcggttact 
      tggactcga aattacatt ggatttttc ggtggtaac 
      cccaacaac attgtggta atgggtcat ggtaccggt 
      gctagtagc gccatcatg gtcgcttta tctaacgtt 
      gcgaagggc ttagccagc ggaattgta gcaatgtct 
      gggagttca ctgtcccaa tttgccact gacgacgct 
      cctgccaac actgccatg gatgtggcc gaagccaat 
      gggtgtcca gttgggtcc gaactgacc atggttcgg 
      tgtctgcag aatttgtct cctgctgca ataataaaa 
      gtcgattct gttattgaa gccacgcga ttacaaaat 
      cgcggtttt gtttccggt cttgctgga aaacttgga 
      tccgctcca gtgtttgaa ggtcgaagg gatggtcgt 
      tcgttgcca cccgttgca gaggttgaa cctgtgaac 
      gatccagaa agtacccaa aagaaaata ccgctattg 
      accgggata acgaaagat gagactaag agggcttgc 
      catgggcaa ttcagggat gaaattacc aataaattg 
      caaaaagtt cccgaattt ttggataag gtacttgtc 
      aagaacctt cagaacgca attgggata aatcgggac 
      aagaattca acttctggc aagttgcta aatcttttg 
      gatcccaac caatttaaa aactatctt caatataag 
      agaaataat cttcatgag ggtcttgcc aaaatagcg 
      gaagctaca gcggatgcg ttattcaac gctccagct 
      ttcctgacc gctgatgct tggtccaaa catggtgca 
      ccggcattt ttgtaccgg tttgaacac gttgggaaa 
      cgaaggaaa gggtacaat tttttgaaa ggattacca 
      cttgtcgga aatcattct cagagtgat gacgtttcc 
      aacgacaca gttgcccac ggcgatgag ctttcttac 
      atcttcgac gctcaagat atggaaggc aagtcgtta 
      gaatcggac acaggcaac gaagaggac aacaaagtc 
      cgtgatatc ttcactcag atgatttcg gattttgct 
      cgtcatgga aaactgagt gtcgaaggc aaacacgtg 
      aaacccttt tccatcacc gacaacaat tttgtgcaa 
      atttcgcct aaaccaaaa gtgtcgaat ggttttaga 
      tactgcgaa atggggctt tggttgggc ttggctcaa 
      agattgcaa agtagcact tgtgaactc ttcaaagtt 
      ttggataca caactgaag aacattgac ttgggcggt 
      ggtacgaaa aaaattgga gagaccatt acgggcatt 
      gtcaaagga ggaggaagc aagaaacca ccagatgta 
      atctcaaaa tcagcaggc cttttaagt gggaataaa 
      gacaaaaag gtgtttgga attggggga tgaagctat 
      aattaatat caaatatgc ttttattat cactataaa 
      aatggcttt cttgtaaca taataaata cacctattt 
      taaaaacta tcgtactct taatctttt gtaaacgaa 
      gtttgatag ccataaggt tccagtgaa aaaatgcaa 
      aactgccac gagacggaa acaggtctt gaatgtagc 
      atctaaaaa cgga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY