Status
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Gene information
General information
TC033864
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008710 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 14526403..14533977
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033864
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01395

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     metathorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen constricted - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033864 RA
      MRTLVSLMW VRQLVGKQV VVVTIQYRL GSLGFLSTG 
TKTLPGNAG LWDMVLAVT WTRNYIGFF GGNPNNIVV 
MGHGTGASS AIMVALSNV AKGLASGIV AMSGSSLSQ 
FATDDAPAN TAMDVAEAN GCPVGSELT MVRCLQNLS 
PAAIIKVDS VIEATRLQN RGFVSGLAG KLGSAPVFE 
GRRDGRSLP PVAEVEPVN DPESTQKKI PLLTGITKD 
ETKRACHGQ FRDEITNKL QKVPEFLDK VLVKNLQNA 
IGINRDKNS TSGKLLNLL DPNQFKNYL QYKRNNLHE 
GLAKIAEAT ADALFNAPA FLTADAWSK HGAPAFLYR 
FEHVGKRRK GYNFLKGLP LVGNHSQSD DVSNDTVAH 
GDELSYIFD AQDMEGKSL ESDTGNEED NKVRDIFTQ 
MISDFARHG KLSVEGKHV KPFSITDNN FVQISPKPK 
VSNGFRYCE MGLWLGLAQ RLQSSTCEL FKVLDTQLK 
NIDLGGGTK KIGETITGI VKGGGSKKP PDVISKSAG 
LLSGNKDKK VFGIGG
    CDS sequence
    • >TC033864 RA
      atgaggact ctcgttagt ctcatgtgg gttcggcaa 
ttggtcggt aagcaggtc gtggttgtg accatccaa 
taccgctta ggctccttg ggtttccta agtactgga 
accaagacc cttcctgga aacgccggg ctatgggat 
atggtcttg gcggttact tggactcga aattacatt 
ggatttttc ggtggtaac cccaacaac attgtggta 
atgggtcat ggtaccggt gctagtagc gccatcatg 
gtcgcttta tctaacgtt gcgaagggc ttagccagc 
ggaattgta gcaatgtct gggagttca ctgtcccaa 
tttgccact gacgacgct cctgccaac actgccatg 
gatgtggcc gaagccaat gggtgtcca gttgggtcc 
gaactgacc atggttcgg tgtctgcag aatttgtct 
cctgctgca ataataaaa gtcgattct gttattgaa 
gccacgcga ttacaaaat cgcggtttt gtttccggt 
cttgctgga aaacttgga tccgctcca gtgtttgaa 
ggtcgaagg gatggtcgt tcgttgcca cccgttgca 
gaggttgaa cctgtgaac gatccagaa agtacccaa 
aagaaaata ccgctattg accgggata acgaaagat 
gagactaag agggcttgc catgggcaa ttcagggat 
gaaattacc aataaattg caaaaagtt cccgaattt 
ttggataag gtacttgtc aagaacctt cagaacgca 
attgggata aatcgggac aagaattca acttctggc 
aagttgcta aatcttttg gatcccaac caatttaaa 
aactatctt caatataag agaaataat cttcatgag 
ggtcttgcc aaaatagcg gaagctaca gcggatgcg 
ttattcaac gctccagct ttcctgacc gctgatgct 
tggtccaaa catggtgca ccggcattt ttgtaccgg 
tttgaacac gttgggaaa cgaaggaaa gggtacaat 
tttttgaaa ggattacca cttgtcgga aatcattct 
cagagtgat gacgtttcc aacgacaca gttgcccac 
ggcgatgag ctttcttac atcttcgac gctcaagat 
atggaaggc aagtcgtta gaatcggac acaggcaac 
gaagaggac aacaaagtc cgtgatatc ttcactcag 
atgatttcg gattttgct cgtcatgga aaactgagt 
gtcgaaggc aaacacgtg aaacccttt tccatcacc 
gacaacaat tttgtgcaa atttcgcct aaaccaaaa 
gtgtcgaat ggttttaga tactgcgaa atggggctt 
tggttgggc ttggctcaa agattgcaa agtagcact 
tgtgaactc ttcaaagtt ttggataca caactgaag 
aacattgac ttgggcggt ggtacgaaa aaaattgga 
gagaccatt acgggcatt gtcaaagga ggaggaagc 
aagaaacca ccagatgta atctcaaaa tcagcaggc 
cttttaagt gggaataaa gacaaaaag gtgtttgga 
attggggga tga
    mRNA sequence
    • >TC033864 RA
      ggaatctaa tgtaacctc atgaggact ctcgttagt 
ctcatgtgg gttcggcaa ttggtcggt aagcaggtc 
gtggttgtg accatccaa taccgctta ggctccttg 
ggtttccta agtactgga accaagacc cttcctgga 
aacgccggg ctatgggat atggtcttg gcggttact 
tggactcga aattacatt ggatttttc ggtggtaac 
cccaacaac attgtggta atgggtcat ggtaccggt 
gctagtagc gccatcatg gtcgcttta tctaacgtt 
gcgaagggc ttagccagc ggaattgta gcaatgtct 
gggagttca ctgtcccaa tttgccact gacgacgct 
cctgccaac actgccatg gatgtggcc gaagccaat 
gggtgtcca gttgggtcc gaactgacc atggttcgg 
tgtctgcag aatttgtct cctgctgca ataataaaa 
gtcgattct gttattgaa gccacgcga ttacaaaat 
cgcggtttt gtttccggt cttgctgga aaacttgga 
tccgctcca gtgtttgaa ggtcgaagg gatggtcgt 
tcgttgcca cccgttgca gaggttgaa cctgtgaac 
gatccagaa agtacccaa aagaaaata ccgctattg 
accgggata acgaaagat gagactaag agggcttgc 
catgggcaa ttcagggat gaaattacc aataaattg 
caaaaagtt cccgaattt ttggataag gtacttgtc 
aagaacctt cagaacgca attgggata aatcgggac 
aagaattca acttctggc aagttgcta aatcttttg 
gatcccaac caatttaaa aactatctt caatataag 
agaaataat cttcatgag ggtcttgcc aaaatagcg 
gaagctaca gcggatgcg ttattcaac gctccagct 
ttcctgacc gctgatgct tggtccaaa catggtgca 
ccggcattt ttgtaccgg tttgaacac gttgggaaa 
cgaaggaaa gggtacaat tttttgaaa ggattacca 
cttgtcgga aatcattct cagagtgat gacgtttcc 
aacgacaca gttgcccac ggcgatgag ctttcttac 
atcttcgac gctcaagat atggaaggc aagtcgtta 
gaatcggac acaggcaac gaagaggac aacaaagtc 
cgtgatatc ttcactcag atgatttcg gattttgct 
cgtcatgga aaactgagt gtcgaaggc aaacacgtg 
aaacccttt tccatcacc gacaacaat tttgtgcaa 
atttcgcct aaaccaaaa gtgtcgaat ggttttaga 
tactgcgaa atggggctt tggttgggc ttggctcaa 
agattgcaa agtagcact tgtgaactc ttcaaagtt 
ttggataca caactgaag aacattgac ttgggcggt 
ggtacgaaa aaaattgga gagaccatt acgggcatt 
gtcaaagga ggaggaagc aagaaacca ccagatgta 
atctcaaaa tcagcaggc cttttaagt gggaataaa 
gacaaaaag gtgtttgga attggggga tgaagctat 
aattaatat caaatatgc ttttattat cactataaa 
aatggcttt cttgtaaca taataaata cacctattt 
taaaaacta tcgtactct taatctttt gtaaacgaa 
gtttgatag ccataaggt tccagtgaa aaaatgcaa 
aactgccac gagacggaa acaggtctt gaatgtagc 
atctaaaaa cgga
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