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Gene information
General information
TC008704 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 14410819..14414973
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008704
    iBeetle screen iB_01394

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008704 RA
      MVQLKLNCS RDDMRESGF CCDPNNPNP CSHKNNGAL 
      VEDPSCNPD SPLKVTFEE ITSAAYKIK SGIINTPCP 
      RSHMSHLTG MDVYLKKDF LQNTGSFKE RGARYAMLM 
      LSPERRQNG VIAASLGNH AQAVCYHGY LLGIPVTVV 
      MPIIAPIMK IQKCKEFNA NVIMEGKDM AEAKKIAIK 
      MAKEKGYTY INGYDHPQI IAGQGTIAL EILEQVEDI 
      DAIVVPTGG GGLLAGVAV AVKTLNPKI KIIGVESER 
      CASFSKAMD AEKPVSTAI EGTLADGLA VPMVGYNAW 
      ETAKSLVDK MVVVKEEWI AVAILRMVE LEKCVVEGA 
      GAVGLAAIL AGQLDEFKG KRVVLILSG GNIDTTILG 
      RCLERGLAA DGRLVKCKV TVSDRPGGI SELCKLIGS 
      IGVSIKDVI HERAWVTSD VFSVEVKVV CETRDYAHS 
      LELRELLFN RYKKVKFEN LPDNLAVSS TSDECIYKQ 
      QCN
    CDS sequence
    • >TC008704 RA
      atggttcaa ttaaagtta aactgcagc cgagacgac 
      atgcgggag tctggcttc tgctgcgac cccaacaac 
      cccaaccct tgtagccac aagaacaat ggggcccta 
      gtggaagac cccagctgc aacccggac tcacccctg 
      aaagtcact ttcgaggaa attacatcg gctgcgtat 
      aaaatcaaa tcggggatt atcaacaca ccatgcccg 
      cgctcccac atgtcgcac ttaaccgga atggatgtt 
      tatttgaaa aaagacttt ttacaaaat acgggaagt 
      tttaaggaa cggggggcg cggtatgcc atgttgatg 
      ttgtcaccg gagcgcaga caaaacgga gtcattgcg 
      gcttcgctc ggaaaccat gcccaagcg gtttgttac 
      cacggctac ctcctggga atccccgtc acggtggtc 
      atgcccata atagccccc ataatgaaa atccagaag 
      tgtaaggaa ttcaacgct aatgttatc atggagggc 
      aaagatatg gccgaagcc aagaaaatc gccattaag 
      atggcgaag gagaaaggt tacacttat atcaacggt 
      tacgaccat ccgcaaatt atcgcaggc caaggcacc 
      atagctttg gaaattttg gagcaagtg gaagacatc 
      gacgctatt gtggtgccc acgggtggg ggcgggctt 
      ttggcgggg gtggcggtc gccgttaag acactgaac 
      cccaaaatt aagattatc ggagttgaa tccgaaagg 
      tgtgcaagt ttttccaaa gccatggat gcggaaaag 
      ccggtgtcc acggctatt gaggggaca ctggcggat 
      gggttggcg gtaccaatg gttgggtac aacgcatgg 
      gagacggca aaatctcta gttgataag atggtcgta 
      gtgaaagaa gagtggatc gcagtggcg atcttgagg 
      atggtcgag ttggaaaag tgtgtcgtg gagggtgct 
      ggggctgtt gggttggct gctattttg gcgggacaa 
      cttgatgag ttcaagggg aaaagagtt gtgttaatt 
      ttgagcggg ggcaacatc gacaccaca attttgggt 
      cggtgttta gaacgtggc ctggcagcc gatggccga 
      cttgtcaaa tgtaaagtt actgtaagc gatagacct 
      ggaggtatt tccgaatta tgtaaactg atcggttcc 
      attggggtt tccatcaaa gacgtcata catgaaagg 
      gcttgggtc acctctgat gttttcagt gttgaggta 
      aaagttgtg tgtgaaact cgggattat gcccatagt 
      ttggaactg agagaacta ttgttcaat cgatataaa 
      aaggttaaa tttgaaaat ttgccagac aatctggcg 
      gtctcgtcg actagtgac gaatgcata tacaagcaa 
      caatgtaat taa
    mRNA sequence
    • >TC008704 RA
      caccacaaa aaagaactg tgatagact gtaattaat 
      gactggata gttttgaga taacgagtc gaccccagg 
      agtctttta tcaacacta aatgtggaa aaatccgct 
      ttatttgtg cgagaaaag ttcacttgc cctatttac 
      ccacataaa aacagtcaa taaacacgt tcattttta 
      atcaatcat ggttcaatt aaagttaaa ctgcagccg 
      agacgacat gcgggagtc tggcttctg ctgcgaccc 
      caacaaccc caacccttg tagccacaa gaacaatgg 
      ggccctagt ggaagaccc cagctgcaa cccggactc 
      acccctgaa agtcacttt cgaggaaat tacatcggc 
      tgcgtataa aatcaaatc ggggattat caacacacc 
      atgcccgcg ctcccacat gtcgcactt aaccggaat 
      ggatgttta tttgaaaaa agacttttt acaaaatac 
      gggaagttt taaggaacg gggggcgcg gtatgccat 
      gttgatgtt gtcaccgga gcgcagaca aaacggagt 
      cattgcggc ttcgctcgg aaaccatgc ccaagcggt 
      ttgttacca cggctacct cctgggaat ccccgtcac 
      ggtggtcat gcccataat agcccccat aatgaaaat 
      ccagaagtg taaggaatt caacgctaa tgttatcat 
      ggagggcaa agatatggc cgaagccaa gaaaatcgc 
      cattaagat ggcgaagga gaaaggtta cacttatat 
      caacggtta cgaccatcc gcaaattat cgcaggcca 
      aggcaccat agctttgga aattttgga gcaagtgga 
      agacatcga cgctattgt ggtgcccac gggtggggg 
      cgggctttt ggcgggggt ggcggtcgc cgttaagac 
      actgaaccc caaaattaa gattatcgg agttgaatc 
      cgaaaggtg tgcaagttt ttccaaagc catggatgc 
      ggaaaagcc ggtgtccac ggctattga ggggacact 
      ggcggatgg gttggcggt accaatggt tgggtacaa 
      cgcatggga gacggcaaa atctctagt tgataagat 
      ggtcgtagt gaaagaaga gtggatcgc agtggcgat 
      cttgaggat ggtcgagtt ggaaaagtg tgtcgtgga 
      gggtgctgg ggctgttgg gttggctgc tattttggc 
      gggacaact tgatgagtt caaggggaa aagagttgt 
      gttaatttt gagcggggg caacatcga caccacaat 
      tttgggtcg gtgtttaga acgtggcct ggcagccga 
      tggccgact tgtcaaatg taaagttac tgtaagcga 
      tagacctgg aggtatttc cgaattatg taaactgat 
      cggttccat tggggtttc catcaaaga cgtcataca 
      tgaaagggc ttgggtcac ctctgatgt tttcagtgt 
      tgaggtaaa agttgtgtg tgaaactcg ggattatgc 
      ccatagttt ggaactgag agaactatt gttcaatcg 
      atataaaaa ggttaaatt tgaaaattt gccagacaa 
      tctggcggt ctcgtcgac tagtgacga atgcatata 
      caagcaaca atgtaatta attgtacct gtgactata 
      ctattaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY