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Gene information
General information
TC008700 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 14340478..14344887
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC008700
      iBeetle screen iB_01393

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC008700 RA
        MNSDGTQRS GVNKDNLPS SSQVTNPDS NANLSLTTN 
        ESKGAIPKT KLAKIASKK TNNNVDETS VLTVDPLKI 
        NGFTKNTSP SSNYLNNTY VECVAANVQ ISSKDVHKG 
        TGFAQSSLN IPVENDFLN GPYLPVHNC GSSYGDLVE 
        AGASYREPN LKDDDSSSD DNELLSLSD DGCIYTYKG 
        DHVADLPSS FYNLEIPPL DEVPGNGRE ENSSPEMDF 
        LEMDFDPGP SGDADSDSI SNAEIDNAA SLPLDSKTE 
        KVEEPKPCC SKDLNNDLP PESSNINKF DNGVKSKYL 
        NLTKTIPEP IKEPIIAIK NEIKMPWSC TLSERTRTA 
        KSLRMVKRH HSSRGELSS PSEIGLNND INITNLTSF 
        HNATGKILI DDKNSVDCD VEKTMIWTH HEACVKQIT 
        QVGPSACGA TAVLNVLNA LRFPIPTLE KVQECVNTR 
        LRANSSPLT EYLLSRSQA GCTHRDLIV GLYKLSEKR 
        LYSRFFAMY PERVVNLQS WLGYWIKNG AVPIATLNL 
        QKCEGSIPD SWHHQMIFG VGPEGIYLT NPLECIDPA 
        ELWPQLSSE SVLLVRTED VISRWNPKV DLPQLMRIN 
        DVRWKELNV VGQIAKVVR ESRREARAD YISTSHIQI 
        PAEYKAGIT LVLDINSPA FTLLRQCPE LPLLQPEYA 
        ASAL
      CDS sequence
      • >TC008700 RA
        atgaattca gatggtacc caaagaagt ggggttaac 
        aaagacaat ttaccatca agcagccaa gtaacgaat 
        ccggacagc aatgctaac ttaagcctg acaacaaac 
        gaaagtaaa ggggccatc cccaaaaca aaactggcg 
        aaaatagcc tcgaaaaag accaacaac aatgtggac 
        gagacaagt gtgttgacg gtggacccg ttgaaaatt 
        aacggcttt acaaagaac acttcgcct tctagcaat 
        tacttgaac aatacgtac gtagaatgc gtcgcagcg 
        aatgtccaa atcagctcg aaagatgtg cataaaggc 
        acggggttt gcacagagc tcgttaaat atcccagta 
        gagaatgat ttcttgaat ggaccgtac cttccggtg 
        cataactgt ggctcgagc tatggcgat ttggtcgaa 
        gctggtgct agttatagg gaaccaaat ttgaaagat 
        gatgacagt tctagtgat gataatgaa ttattgtcc 
        ttgtccgat gatgggtgc atttatact tataagggg 
        gatcatgtg gcggacttg ccaagctcc ttttacaac 
        ctggaaata ccaccgctg gacgaggtg ccaggaaat 
        gggcgggaa gaaaactcc agtcccgag atggacttc 
        ctggaaatg gacttcgac cccggacct tcgggagac 
        gccgactct gactccata tcaaacgcc gaaatcgac 
        aacgcggca agtttaccc ttagactcg aagaccgaa 
        aaagtcgaa gaaccaaaa ccatgttgt tcaaaagat 
        ttaaacaac gacctcccc ccagaatca agcaacatc 
        aacaaattc gacaacggt gttaaaagc aaatatctc 
        aatttgacc aaaacaata ccggagcca ataaaagag 
        ccaatcatt gcgataaaa aacgaaata aaaatgccg 
        tggtcttgc acgctctcc gagcgcact cgaacggca 
        aaatccctt cgaatggtc aaaagacac cacagttcc 
        agaggcgag ctctcgtcc ccgagcgaa atcgggcta 
        aacaacgac atcaacatc accaatcta acgtcgttt 
        cataacgcc accgggaaa attttaatc gatgataaa 
        aactcggtg gactgtgat gtggaaaaa acaatgatc 
        tggacccat cacgaggcc tgtgtcaag cagataacc 
        caagtgggg ccctcggcc tgtggcgcc accgcagtt 
        ctcaacgtt ctcaacgcg cttaggttt cccatcccg 
        acgttggaa aaggtccaa gagtgtgta aatacaaga 
        ctgagggcc aattccagt cctctgacc gagtatttg 
        ctttcgagg agtcaagcc ggttgcact caccgggac 
        ttgatcgtt ggattgtat aaactatca gagaaaaga 
        ctctactcg aggtttttt gcaatgtat cccgaaagg 
        gtggtcaat ttgcagtcg tggcttggt tattggata 
        aaaaatggt gctgtgcca atcgccacg ctgaattta 
        caaaaatgc gaagggagt attcctgac agttggcac 
        caccagatg atttttggg gtgggcccg gaagggatt 
        tatctaacg aatccactg gaatgtatc gatccggcg 
        gagttgtgg ccgcagttg tcgtcggag tccgtgctg 
        ctggttagg accgaagac gtgatttcc aggtggaat 
        ccgaaggtg gatctacct cagctaatg aggattaat 
        gacgtgcgg tggaaggaa ctgaatgtt gtaggtcaa 
        attgcgaaa gttgtgcgt gagagccgc agagaggcc 
        cgcgctgac tacatttcc acatcgcat attcaaatc 
        ccagccgag tacaaagcc gggatcaca ctcgttctg 
        gacataaac tcccccgct tttacactg ttacgtcag 
        tgccccgaa ctgcctttg ctgcagccc gagtatgca 
        gcgagtgcc ttgtaa
      mRNA sequence
      • >TC008700 RA
        gcggtgata aaaataaat aattgtcaa tttcagaat 
        gtttctaat tagtgacag taattattt atgaattgt 
        tgcaaaaag aagcacatt ctatcagcc gttatcggc 
        cgtgttccg tttttgagt taaatgttg ccaaaccca 
        gtcaattta caatgaatt cagatggta cccaaagaa 
        gtggggtta acaaagaca atttaccat caagcagcc 
        aagtaacga atccggaca gcaatgcta acttaagcc 
        tgacaacaa acgaaagta aaggggcca tccccaaaa 
        caaaactgg cgaaaatag cctcgaaaa agaccaaca 
        acaatgtgg acgagacaa gtgtgttga cggtggacc 
        cgttgaaaa ttaacggct ttacaaaga acacttcgc 
        cttctagca attacttga acaatacgt acgtagaat 
        gcgtcgcag cgaatgtcc aaatcagct cgaaagatg 
        tgcataaag gcacggggt ttgcacaga gctcgttaa 
        atatcccag tagagaatg atttcttga atggaccgt 
        accttccgg tgcataact gtggctcga gctatggcg 
        atttggtcg aagctggtg ctagttata gggaaccaa 
        atttgaaag atgatgaca gttctagtg atgataatg 
        aattattgt ccttgtccg atgatgggt gcatttata 
        cttataagg gggatcatg tggcggact tgccaagct 
        ccttttaca acctggaaa taccaccgc tggacgagg 
        tgccaggaa atgggcggg aagaaaact ccagtcccg 
        agatggact tcctggaaa tggacttcg accccggac 
        cttcgggag acgccgact ctgactcca tatcaaacg 
        ccgaaatcg acaacgcgg caagtttac ccttagact 
        cgaagaccg aaaaagtcg aagaaccaa aaccatgtt 
        gttcaaaag atttaaaca acgacctcc ccccagaat 
        caagcaaca tcaacaaat tcgacaacg gtgttaaaa 
        gcaaatatc tcaatttga ccaaaacaa taccggagc 
        caataaaag agccaatca ttgcgataa aaaacgaaa 
        taaaaatgc cgtggtctt gcacgctct ccgagcgca 
        ctcgaacgg caaaatccc ttcgaatgg tcaaaagac 
        accacagtt ccagaggcg agctctcgt ccccgagcg 
        aaatcgggc taaacaacg acatcaaca tcaccaatc 
        taacgtcgt ttcataacg ccaccggga aaattttaa 
        tcgatgata aaaactcgg tggactgtg atgtggaaa 
        aaacaatga tctggaccc atcacgagg cctgtgtca 
        agcagataa cccaagtgg ggccctcgg cctgtggcg 
        ccaccgcag ttctcaacg ttctcaacg cgcttaggt 
        ttcccatcc cgacgttgg aaaaggtcc aagagtgtg 
        taaatacaa gactgaggg ccaattcca gtcctctga 
        ccgagtatt tgctttcga ggagtcaag ccggttgca 
        ctcaccggg acttgatcg ttggattgt ataaactat 
        cagagaaaa gactctact cgaggtttt ttgcaatgt 
        atcccgaaa gggtggtca atttgcagt cgtggcttg 
        gttattgga taaaaaatg gtgctgtgc caatcgcca 
        cgctgaatt tacaaaaat gcgaaggga gtattcctg 
        acagttggc accaccaga tgatttttg gggtgggcc 
        cggaaggga tttatctaa cgaatccac tggaatgta 
        tcgatccgg cggagttgt ggccgcagt tgtcgtcgg 
        agtccgtgc tgctggtta ggaccgaag acgtgattt 
        ccaggtgga atccgaagg tggatctac ctcagctaa 
        tgaggatta atgacgtgc ggtggaagg aactgaatg 
        ttgtaggtc aaattgcga aagttgtgc gtgagagcc 
        gcagagagg cccgcgctg actacattt ccacatcgc 
        atattcaaa tcccagccg agtacaaag ccgggatca 
        cactcgttc tggacataa actcccccg cttttacac 
        tgttacgtc agtgccccg aactgcctt tgctgcagc 
        ccgagtatg cagcgagtg ccttgtaaa atagttgca 
        gtgaatctt caccaactc taattttgc gctaagtgc 
        cataattag gttccaaat cccattttg ttgtgaccg 
        aaattgaac ttttcgtac ttacatatc acacagcta 
        agtattgat ttgttacat cagttgatt tcatgtaca 
        tacctacac atatactca tatcatagg tagggttgt 
        tattctgta caaactaat tttaacgca ataatcttg 
        ttaatgtag t
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY