Status
Please wait ...
Gene information
General information
TC008697 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 14209508..14210907
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008697
    iBeetle screen iB_01392

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature mostly fluorescence not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    legs segment shortened - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    legs segment potentially fused - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen circumference decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen bent - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen circumference increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008697 RA
      MVSLSRSFN TNSGGATFF SESGNMGDP EHLQPILES 
      YGTFRPLNT VPVFSLHLL FTLALEIVA IVFAVQHPD 
      EKYKCREYF IIIYIHAGL WFLTLIVDQ IARRKHYNL 
      RILGYLEFY NKTHIHHRL PLYVVSLWN AVIMIIQAI 
      AQQFYPDNF AEKCINGGT MSPIGYLCA LITFEFCLI 
      AGININYIF KVQRFNKQK APPDVQREE WNASMSPEA 
      TEIGSPLRG EKLYDFLQK QADLIRFLK EHNAKLGEK 
      LMVLSAQMQ ARG
    CDS sequence
    • >TC008697 RA
      atggttagt ttatcaaga agtttcaac accaattca 
      ggcggtgcc acgtttttc agtgaatct ggaaacatg 
      ggtgatcca gagcacctg caacccatt ctagagtct 
      tatggtact ttccggccc ttaaatact gtgccagtt 
      tttagcctg catttgctc ttcacgctg gcgttggaa 
      attgtggct atagtgttc gctgtacag catccagat 
      gaaaagtac aagtgccgg gagtacttt attattatt 
      tatattcat gcggggttg tggtttctg acattgatt 
      gtagatcaa atagcgaga cgtaaacat tacaatctc 
      agaattctg ggttatttg gaattttac aataaaact 
      catattcat catcgtctt ccactgtac gttgttagt 
      ctgtggaat gctgtcatt atgataatc caagccata 
      gcccagcag ttctacccc gacaatttt gctgaaaag 
      tgcatcaat ggggggaca atgtctccc ataggttat 
      ctttgtgcc ttgataact tttgagttt tgtttaatt 
      gcgggcatt aacattaat tacattttt aaagttcag 
      cggtttaat aaacaaaaa gcgcccccg gatgtgcaa 
      agagaggag tggaatgct tccatgagt cctgaagct 
      acagaaata gggagtcct ttaagggga gagaaactg 
      tacgacttt ctacagaaa caggctgat ttgataagg 
      tttttgaaa gaacacaac gcaaaatta ggcgaaaag 
      ttgatggtc ttgagtgct cagatgcaa gcacgagga 
      tga
    mRNA sequence
    • >TC008697 RA
      ttacttgcg ataaacaaa taattgcgc acgcagtgt 
      catgttttt cgaataaat cttcgaatc ttccgtaaa 
      tattaatgt cccagtttg aaattaaac tagtgcaat 
      aattaaaaa aatggttag tttatcaag aagtttcaa 
      caccaattc aggcggtgc cacgttttt cagtgaatc 
      tggaaacat gggtgatcc agagcacct gcaacccat 
      tctagagtc ttatggtac tttccggcc cttaaatac 
      tgtgccagt ttttagcct gcatttgct cttcacgct 
      ggcgttgga aattgtggc tatagtgtt cgctgtaca 
      gcatccaga tgaaaagta caagtgccg ggagtactt 
      tattattat ttatattca tgcggggtt gtggtttct 
      gacattgat tgtagatca aatagcgag acgtaaaca 
      ttacaatct cagaattct gggttattt ggaatttta 
      caataaaac tcatattca tcatcgtct tccactgta 
      cgttgttag tctgtggaa tgctgtcat tatgataat 
      ccaagccat agcccagca gttctaccc cgacaattt 
      tgctgaaaa gtgcatcaa tggggggac aatgtctcc 
      cataggtta tctttgtgc cttgataac ttttgagtt 
      ttgtttaat tgcgggcat taacattaa ttacatttt 
      taaagttca gcggtttaa taaacaaaa agcgccccc 
      ggatgtgca aagagagga gtggaatgc ttccatgag 
      tcctgaagc tacagaaat agggagtcc tttaagggg 
      agagaaact gtacgactt tctacagaa acaggctga 
      tttgataag gtttttgaa agaacacaa cgcaaaatt 
      aggcgaaaa gttgatggt cttgagtgc tcagatgca 
      agcacgagg atgacacgg gtgttagct taatgttta 
      catttgtta ttgtgatag cgttaatca ttttaatta 
      tatttttat atcagtgaa tgtaaaata attcgttgt 
      atccatgtt ctagtggta gctataata atatcgtta 
      atctaaata aatgaagag atgacttat ataactttt 
      gtatttata atataatta tgaaatacg gaatgactg 
      taattatct ttgtaattg aaattaatc ctctaacac 
      caaagtaac cgattgtaa attatgtaa gagtgtata 
      aattatgaa atcaataaa agaaattgg taaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY