Status
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Gene information
General information
TC008686 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 14028683..14031687
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008686
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01390

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008686 RA
      MPPTTSIKK VLNTKTLKK SRQSNTRVE DGDDLEEWK 
      KSKQLLRPK DQLELSEGE LKDIIARQL GTNDPLIPH 
      NLVQFNYAQ GGFELMPPA GPMIVVYEA QGTQVHKES 
      EEAKKQVVE QGLDPAILE ISQSTETLA VEEPIPVIP 
      EEVIEEVAA EESEKPEEE TEAEEGEGE GEGEGEGEG 
      EGEGESIRP EDDEDKPQT GGKPKKLTN QFNFCERAA 
      LTYNNPMRT QETQTIPPP MGTFSANVL QWIIYDAYQ 
      EDFEAQQLE KELERERKE KEKAPVSRH QAMAKKLVG 
      KAHQLSEAV QGRVLECWK VLERMVNQN TFDDIAKDY 
      RYWEDPSDE FRDEEGTLL PLWKFHYDK TRKNTVTDI 
      EWNPHYYDL FAVCFGFLD FMKPLEEGA VCLFTLKNP 
      SFPDYICMT DSAVMCVDT HPKYPYMVV IGLYDGSVS 
      VYNVQATCK QPAFRSNNV TNKHRGIVW EVKWAPDLP 
      DGEMNFYSV SEDGKINNW VLMQNDLAV TTIITLFLA 
      KDEVFGPDG TKLKVKGCA SCVKFHPKQ PLIYLVGTE 
      EGHIYKCST AYSSMFLLT YEAHHMPVY RIDFNRYNS 
      DIFISCSGD WRIKIWEDN RLEPLFVFD VGACVGDVK 
      WAPYSSTVF AAVTSEGKV YVFDLNVNK YKPICVQAI 
      VSKRRNKLT RISFNPKLP IIIVGDDKG CVQTLKLSP 
      NLRIPCKAP KKQQHLDQW TLQCMKLDK LLSLVREPG 
      ALDLPEDTA ESIES
    CDS sequence
    • >TC008686 RA
      atgcctcca acaacgtcc atcaaaaaa gttttaaat 
      acaaaaact ttgaaaaaa agtcgccaa agtaacact 
      cgcgtcgag gacggagac gacttagaa gaatggaaa 
      aagtccaag cagttgtta cgcccaaaa gaccagctc 
      gaattaagc gaaggagaa ttaaaagac ataatcgcc 
      cgacagtta ggaacaaat gaccctcta attccgcac 
      aatttggtc caattcaac tatgcccaa ggagggttc 
      gagctgatg ccacctgca ggccctatg attgttgtt 
      tacgaagcg caaggcacc caagtgcat aaggagtca 
      gaggaggcg aaaaaacaa gtcgttgag caaggactt 
      gaccctgcg attttggaa atcagccaa agtaccgaa 
      acactcgcg gtggaagaa ccaatcccg gtaatcccc 
      gaggaagta attgaagaa gtcgcagct gaagaaagt 
      gaaaaaccg gaggaggaa accgaagct gaggaaggc 
      gaaggtgag ggcgagggc gaaggagag ggtgagggc 
      gaaggcgaa ggcgaaagt atcaggcct gaggatgac 
      gaggataaa ccgcaaact ggaggaaaa ccgaaaaaa 
      ttaaccaat cagtttaac ttttgcgag cgcgctgcg 
      cttacgtat aataatcca atgcggacg caggaaaca 
      caaactatt cctccaccc atggggact ttctcggct 
      aatgtttta cagtggata atttacgat gcttaccag 
      gaggatttt gaggcacag cagttggaa aaagagctt 
      gagagggaa cggaaggag aaggaaaaa gcgccggtt 
      tccagacac caagccatg gccaaaaaa ctggtcggg 
      aaagcgcat cagttgagc gaagcggtc caaggacgg 
      gtcttggaa tgttggaag gtcttggag cggatggtt 
      aaccaaaat actttcgat gatattgca aaagattac 
      aggtattgg gaggacccg tctgacgaa tttcgagat 
      gaggagggc actttgttg cctttgtgg aagttccat 
      tacgataaa acgcggaaa aatacagtt actgatatt 
      gagtggaac ccgcactat tacgacttg tttgcagtt 
      tgcttcggt ttcttggat tttatgaaa ccgctagaa 
      gaaggcgct gtttgtctt ttcacttta aaaaatccc 
      tcatttccc gactatatc tgcatgacg gattccgca 
      gtcatgtgt gtcgatacc caccctaaa tatccttac 
      atggtggta ataggatta tatgatggt agtgttagt 
      gtttacaac gttcaagcc acttgtaaa caacctgcc 
      tttagaagc aataatgtc acaaataag cacagaggc 
      attgtgtgg gaagttaaa tgggcacct gatttgccc 
      gacggtgaa atgaatttt tactcggtg tcggaggat 
      ggcaaaatc aataactgg gttttaatg caaaatgat 
      ttagcggtg acgactatt attaccctg tttttggcc 
      aaagatgaa gtttttggg cccgatgga acgaaatta 
      aaagtgaaa ggttgcgct tcatgtgtt aagtttcat 
      cctaaacaa cctctcatt tatttggtt ggcacggaa 
      gaaggccat atctacaaa tgtagtact gcctacagt 
      tctatgttt ctcctaact tacgaagca caccacatg 
      ccggtctat agaatagat tttaatcgg tataattct 
      gatattttt atatcgtgt agtggtgac tggagaatt 
      aaaatttgg gaggacaac agattggag ccacttttc 
      gttttcgat gttggcgct tgtgttggc gacgtaaag 
      tgggcccct tattcctca actgtcttt gcagcagta 
      acgagtgaa ggcaaagtc tacgttttt gatttaaac 
      gtaaataaa tacaaaccg atttgtgtc caagcaatt 
      gtatccaaa cggagaaat aaactgaca cggatttcc 
      ttcaatccg aaattacct attataatt gttggagat 
      gacaagggt tgtgtacaa actttgaaa ttgtcaccc 
      aatttaaga attccttgt aaggcaccg aagaagcaa 
      caacattta gatcaatgg acccttcag tgtatgaag 
      ttggacaaa ctgctgtcg ctagtgagg gaaccagga 
      gcgttagat cttcccgag gatacggca gagtcaata 
      gagtcataa
    mRNA sequence
    • >TC008686 RA
      tgtcaagac aacgcgtaa atattgtca gttcatggc 
      ccttggcta atgtcagtg taaatttgt cacttaaat 
      gtaataatc ctcgataaa atgcctcca acaacgtcc 
      atcaaaaaa gttttaaat acaaaaact ttgaaaaaa 
      agtcgccaa agtaacact cgcgtcgag gacggagac 
      gacttagaa gaatggaaa aagtccaag cagttgtta 
      cgcccaaaa gaccagctc gaattaagc gaaggagaa 
      ttaaaagac ataatcgcc cgacagtta ggaacaaat 
      gaccctcta attccgcac aatttggtc caattcaac 
      tatgcccaa ggagggttc gagctgatg ccacctgca 
      ggccctatg attgttgtt tacgaagcg caaggcacc 
      caagtgcat aaggagtca gaggaggcg aaaaaacaa 
      gtcgttgag caaggactt gaccctgcg attttggaa 
      atcagccaa agtaccgaa acactcgcg gtggaagaa 
      ccaatcccg gtaatcccc gaggaagta attgaagaa 
      gtcgcagct gaagaaagt gaaaaaccg gaggaggaa 
      accgaagct gaggaaggc gaaggtgag ggcgagggc 
      gaaggagag ggtgagggc gaaggcgaa ggcgaaagt 
      atcaggcct gaggatgac gaggataaa ccgcaaact 
      ggaggaaaa ccgaaaaaa ttaaccaat cagtttaac 
      ttttgcgag cgcgctgcg cttacgtat aataatcca 
      atgcggacg caggaaaca caaactatt cctccaccc 
      atggggact ttctcggct aatgtttta cagtggata 
      atttacgat gcttaccag gaggatttt gaggcacag 
      cagttggaa aaagagctt gagagggaa cggaaggag 
      aaggaaaaa gcgccggtt tccagacac caagccatg 
      gccaaaaaa ctggtcggg aaagcgcat cagttgagc 
      gaagcggtc caaggacgg gtcttggaa tgttggaag 
      gtcttggag cggatggtt aaccaaaat actttcgat 
      gatattgca aaagattac aggtattgg gaggacccg 
      tctgacgaa tttcgagat gaggagggc actttgttg 
      cctttgtgg aagttccat tacgataaa acgcggaaa 
      aatacagtt actgatatt gagtggaac ccgcactat 
      tacgacttg tttgcagtt tgcttcggt ttcttggat 
      tttatgaaa ccgctagaa gaaggcgct gtttgtctt 
      ttcacttta aaaaatccc tcatttccc gactatatc 
      tgcatgacg gattccgca gtcatgtgt gtcgatacc 
      caccctaaa tatccttac atggtggta ataggatta 
      tatgatggt agtgttagt gtttacaac gttcaagcc 
      acttgtaaa caacctgcc tttagaagc aataatgtc 
      acaaataag cacagaggc attgtgtgg gaagttaaa 
      tgggcacct gatttgccc gacggtgaa atgaatttt 
      tactcggtg tcggaggat ggcaaaatc aataactgg 
      gttttaatg caaaatgat ttagcggtg acgactatt 
      attaccctg tttttggcc aaagatgaa gtttttggg 
      cccgatgga acgaaatta aaagtgaaa ggttgcgct 
      tcatgtgtt aagtttcat cctaaacaa cctctcatt 
      tatttggtt ggcacggaa gaaggccat atctacaaa 
      tgtagtact gcctacagt tctatgttt ctcctaact 
      tacgaagca caccacatg ccggtctat agaatagat 
      tttaatcgg tataattct gatattttt atatcgtgt 
      agtggtgac tggagaatt aaaatttgg gaggacaac 
      agattggag ccacttttc gttttcgat gttggcgct 
      tgtgttggc gacgtaaag tgggcccct tattcctca 
      actgtcttt gcagcagta acgagtgaa ggcaaagtc 
      tacgttttt gatttaaac gtaaataaa tacaaaccg 
      atttgtgtc caagcaatt gtatccaaa cggagaaat 
      aaactgaca cggatttcc ttcaatccg aaattacct 
      attataatt gttggagat gacaagggt tgtgtacaa 
      actttgaaa ttgtcaccc aatttaaga attccttgt 
      aaggcaccg aagaagcaa caacattta gatcaatgg 
      acccttcag tgtatgaag ttggacaaa ctgctgtcg 
      ctagtgagg gaaccagga gcgttagat cttcccgag 
      gatacggca gagtcaata gagtcataa gcgtatttt 
      atcggtaaa ctttattaa cattgaata aacttctac 
      ataaatatc taaacaata ttattcaac aaaacgctt 
      tggcacata gtcatgtaa cataaaata gagttcacg 
      atgttcaca tctgaagaa ttgagcaac taattaggt 
      acaatagtc gttaaataa ccacttgtt taatgaaag 
      tgattgaac aaattgcct ggtacatct aataaaatt 
      attttctga atcggattt tttttaagt cttagctag 
      agtacggag aattaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY