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Gene information
General information
TC008685 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 13973273..13975771
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008685
    iBeetle screen iB_01389

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larval musculature mostly fluorescence not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva cuticle dorsal irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head capsule mostly not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    vertex triplet irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008685 RA
      MAFLRNILA KQSPITNAF TNTIWHRQM SGGNPVECK 
      VAVITGGAI GIGVACAKS LLENGARGV MLGDIREKE 
      GKELAAELN KTYGKDKAL FMKCDVTKK DEFENLFKC 
      TYQRFPSMD IVINNAGLL QDKYWPLEL AVNVNGLVQ 
      GTLFGFHYM GKHGKHEKG RGGVIVNMA SIYGLQQAY 
      ACPVYNGTK GFVIRFSNA MSHEYYEKM TGVKVLTMC 
      PGVTDTNMI DESSTYALD GFGDLGKLV SEGLANLPP 
      QPASACGDC LIKMLCSGE NGGIWVIEE SECYKVRLP 
      DRQTLRTVS IMFRLLFLK NIRNIASIF LAKRLQWPR 
      NTLQKSSLA RGRNEAQPE QPPKPHKEI FFPQLGRDG 
      IKDKVALVT GGTDGIGLR CVRELLRTG ARGVMIADT 
      NESKGFNRV IEFSKDYGQ SRIAFIQIN PANYVLLKN 
      AFISTLNFY EGLDIVINN VRFKEKNWE DQVDENIKG 
      IVLSTQLCL EFMGRNNGG KGGILLNIV SEYEENTDS 
      CPLHSACQD FVVRFDRSV ATSHFDTTN VKVLTICRA 
      SFDTIGCDL TTILTKGTN GCVWMPCTC ASDS
    CDS sequence
    • >TC008685 RA
      atggctttc ctcaggaat attcttgca aaacagagc 
      ccaataaca aacgcgttt actaacaca atatggcat 
      cgccaaatg tctgggggg aatcctgtc gaatgcaaa 
      gttgctgtt ataactggc ggtgctatt ggcattggg 
      gttgcttgt gctaagtca cttttggaa aacggtgct 
      aggggcgta atgttgggt gatattcga gaaaaagaa 
      ggcaaagaa ctcgcggct gaattaaat aagacctac 
      ggtaaagat aaagccctt ttcatgaaa tgtgacgtc 
      accaaaaag gatgaattt gaaaacttg ttcaaatgc 
      acctatcag aggtttcca agtatggat atcgtaatc 
      aataacgct ggtctgttg caagacaaa tattggccc 
      cttgaacta gccgtgaac gtgaacggc ttggtacaa 
      gggacacta tttggtttc cactacatg ggaaaacat 
      gggaaacac gaaaagggc agaggtgga gttattgtg 
      aacatggcg tccatctat gggctccaa caagcctac 
      gcatgtcct gtttacaac ggcactaaa ggatttgta 
      attcgattt agcaacgct atgtcccat gaatattac 
      gagaaaatg acaggcgtt aaagtcctg acaatgtgt 
      cctggggtg actgacacg aacatgatt gacgaatca 
      agcacctat gccctggat ggctttgga gacctgggc 
      aaacttgtg agcgaagga ctggccaat ctgccaccc 
      caaccagct tcagcttgt ggggactgt ttgatcaaa 
      atgctgtgc tccggcgaa aatgggggt atttgggtg 
      attgaagaa agcgagtgc tacaaagtc cggcttccg 
      gaccgccag actctgcgt accgtttcg ataatgttt 
      aggttgctg tttttaaaa aacattaga aatatcgcc 
      tctattttc ctagccaaa aggctgcaa tggcccaga 
      aacaccttg cagaaatcg tcactggca cggggcaga 
      aacgaggct cagccggaa cagcccccg aagccgcac 
      aaggagata tttttcccg cagttgggg cgcgatggt 
      attaaggac aaagtagct ttagtgact gggggcacg 
      gacggaatt ggcttgcga tgtgtgaga gaattgcta 
      agaaccggg gctaggggg gtaatgatt gcagacaca 
      aacgagagc aagggtttc aatcgggtg atagagttt 
      tcaaaggac tatggccaa agtaggata gcgttcatt 
      cagatcaac ccggctaat tatgttttg cttaaaaat 
      gcgtttatt agcacccta aatttctac gagggcttg 
      gacattgtg attaacaac gtcaggttc aaggaaaaa 
      aattgggag gatcaggtt gatgagaat attaaagga 
      atcgtgttg agtacacag ctctgtttg gaatttatg 
      ggtcgtaat aatggagga aaaggcggg attttgctg 
      aacatagtc tcagaatat gaggaaaac accgactcc 
      tgtccactg cacagtgct tgtcaagac tttgttgtc 
      cgctttgac cgatcagtt gcaacgtca catttcgac 
      accacgaac gtcaaagta ttaacaatt tgcagagcc 
      agttttgac acgatcggg tgcgactta accacgata 
      ctaacaaaa ggaaccaac ggctgtgtt tggatgcct 
      tgcacttgt gcgtcggat tcctaa
    mRNA sequence
    • >TC008685 RA
      tgtttgtgt cgtgcaaaa tggctttcc tcaggaata 
      ttcttgcaa aacagagcc caataacaa acgcgttta 
      ctaacacaa tatggcatc gccaaatgt ctgggggga 
      atcctgtcg aatgcaaag ttgctgtta taactggcg 
      gtgctattg gcattgggg ttgcttgtg ctaagtcac 
      ttttggaaa acggtgcta ggggcgtaa tgttgggtg 
      atattcgag aaaaagaag gcaaagaac tcgcggctg 
      aattaaata agacctacg gtaaagata aagcccttt 
      tcatgaaat gtgacgtca ccaaaaagg atgaatttg 
      aaaacttgt tcaaatgca cctatcaga ggtttccaa 
      gtatggata tcgtaatca ataacgctg gtctgttgc 
      aagacaaat attggcccc ttgaactag ccgtgaacg 
      tgaacggct tggtacaag ggacactat ttggtttcc 
      actacatgg gaaaacatg ggaaacacg aaaagggca 
      gaggtggag ttattgtga acatggcgt ccatctatg 
      ggctccaac aagcctacg catgtcctg tttacaacg 
      gcactaaag gatttgtaa ttcgattta gcaacgcta 
      tgtcccatg aatattacg agaaaatga caggcgtta 
      aagtcctga caatgtgtc ctggggtga ctgacacga 
      acatgattg acgaatcaa gcacctatg ccctggatg 
      gctttggag acctgggca aacttgtga gcgaaggac 
      tggccaatc tgccacccc aaccagctt cagcttgtg 
      gggactgtt tgatcaaaa tgctgtgct ccggcgaaa 
      atgggggta tttgggtga ttgaagaaa gcgagtgct 
      acaaagtcc ggcttccgg accgccaga ctctgcgta 
      ccgtttcga taatgttta ggttgctgt ttttaaaaa 
      acattagaa atatcgcct ctattttcc tagccaaaa 
      ggctgcaat ggcccagaa acaccttgc agaaatcgt 
      cactggcac ggggcagaa acgaggctc agccggaac 
      agcccccga agccgcaca aggagatat ttttcccgc 
      agttggggc gcgatggta ttaaggaca aagtagctt 
      tagtgactg ggggcacgg acggaattg gcttgcgat 
      gtgtgagag aattgctaa gaaccgggg ctagggggg 
      taatgattg cagacacaa acgagagca agggtttca 
      atcgggtga tagagtttt caaaggact atggccaaa 
      gtaggatag cgttcattc agatcaacc cggctaatt 
      atgttttgc ttaaaaatg cgtttatta gcaccctaa 
      atttctacg agggcttgg acattgtga ttaacaacg 
      tcaggttca aggaaaaaa attgggagg atcaggttg 
      atgagaata ttaaaggaa tcgtgttga gtacacagc 
      tctgtttgg aatttatgg gtcgtaata atggaggaa 
      aaggcggga ttttgctga acatagtct cagaatatg 
      aggaaaaca ccgactcct gtccactgc acagtgctt 
      gtcaagact ttgttgtcc gctttgacc gatcagttg 
      caacgtcac atttcgaca ccacgaacg tcaaagtat 
      taacaattt gcagagcca gttttgaca cgatcgggt 
      gcgacttaa ccacgatac taacaaaag gaaccaacg 
      gctgtgttt ggatgcctt gcacttgtg cgtcggatt 
      cctaattag aatacttta attatttgt gtatgaatg 
      aacaaggtg tttttttcc caatgactg tctttttct 
      gtttgaatc aatacaaat taatgaacg ct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY