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Gene information
General information
TC008675 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 13934643..13936490
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008675
    iBeetle screen iB_01387

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008675 RA
      MYKVFIFCL SVKCVLSAN ILYVSPIPS PSHHIWNRA 
      LALGLVNKG HSVTLIGPD KDKVHPKNY SHIYMEGIY 
      EELDDSFDI NEMGTYSPA QMMLEFESW SLFSCEKAL 
      KTQGLQKLL GYPADSFDL IIFDVTTGS CFYPLIQKF 
      NYPPSIAVT AFLLPTYVA HNFGNHLYP SYIPWYGLQ 
      YTSEMSFVE RVWNFIFTY ADVVRRKIS LYQKEHSMA 
      KEIFGENIP SMEELERHI SLVLANTDP ILDFPQPVP 
      PNIIPVGGL HTRKSKDLP QDILTVLDN AKHGIIVFS 
      LGSNLRSDK LNKQTQNAL LEAFSKIQE TVIWKFESD 
      IENLPKNVI VRKWLPQND ILGHPNVKL FIGHGGALS 
      TQEALYHGV PMICVPFIV DQHINTRII VNKNLGIHL 
      DFKKITAGY VLQLLREVL DNPKYTENM KKISNIFRD 
      RLETPLERG VFWVEYVLR HGGAQFLTT PARDFSYFK 
      ACSLDVIAF LFAIATVIV IIVCKMFAL IMKACSSKK 
      KIKVN
    CDS sequence
    • >TC008675 RA
      atgtataaa gtatttatt ttttgttta agtgtaaaa 
      tgtgttcta agcgccaac attttatac gtgtcacct 
      ataccttcc cccagtcac cacatatgg aatcgagct 
      ttagcactt ggactagtc aacaaaggc cacagtgtc 
      actttaata ggacctgac aaagataaa gtacacccg 
      aaaaattat tcccacatt tatatggaa ggtatttac 
      gaagagcta gacgacagt tttgacata aatgaaatg 
      ggcacgtac agcccagca cagatgatg ttggagttc 
      gaaagctgg agtcttttt tcttgtgaa aaagctcta 
      aagacacaa ggattacaa aagttgctg ggttatccc 
      gcagattct ttcgacctt attatattc gatgttacg 
      actggttct tgcttctac cccttaatc cagaaattc 
      aactaccct ccaagtatt gcggtcact gcgtttttg 
      ctaccaaca tacgttgcg cataatttt ggcaatcac 
      ttatatccc tcatatatt ccttggtat gggctccag 
      tacacatca gaaatgagt tttgtggaa agagtttgg 
      aactttata ttcacttat gccgacgtg gttaggaga 
      aaaataagt ttgtatcaa aaggaacac agtatggcc 
      aaagaaatt tttggcgaa aatatcccg tcgatggaa 
      gaacttgaa cggcacata tctttagtt ttggctaac 
      acggatccg attctggat tttcctcaa cctgtccca 
      ccaaacatt atcccggtt ggaggcctc catacaagg 
      aagagtaaa gatttacct caagacatt ctaacagta 
      ttagacaat gccaagcat ggaattata gtcttttca 
      ttaggttca aatctacgt tcagataaa ttaaataaa 
      caaactcaa aacgcattg ctggaagct tttagtaaa 
      attcaagag actgtgatt tggaaattt gaaagtgat 
      atagaaaat ttacctaaa aatgtcatc gttaggaag 
      tggttgccg cagaatgat attctagga caccccaac 
      gtgaaatta tttattgga catggtggt gctttaagc 
      acacaagag gcattatac cacggtgtc cccatgatt 
      tgtgttcct ttcattgtt gaccaacat attaacacg 
      cggataatt gttaacaaa aacctggga attcatctc 
      gattttaag aaaataacc gctggttat gtacttcaa 
      ctgcttaga gaagttttg gataacccc aagtacacg 
      gaaaacatg aaaaagatt tcaaacatt ttcagagac 
      cgtctagag actccttta gaaagaggc gtattttgg 
      gtggaatat gttttacgg catggtgga gcgcaattc 
      ttaacaacc ccagccaga gatttctcg tattttaaa 
      gcatgcagt ctggatgtt attgcattc ctttttgca 
      attgccacg gttatagta attattgtt tgtaaaatg 
      tttgcgtta ataatgaaa gcatgtagt agcaagaaa 
      aaaattaaa gtaaattaa
    mRNA sequence
    • >TC008675 RA
      tgataattc atgatcctt atttcagtc gttaatagc 
      tacagatac gtttactat gtataaagt atttatttt 
      ttgtttaag tgtaaaatg tgttctaag cgccaacat 
      tttatacgt gtcacctat accttcccc cagtcacca 
      catatggaa tcgagcttt agcacttgg actagtcaa 
      caaaggcca cagtgtcac tttaatagg acctgacaa 
      agataaagt acacccgaa aaattattc ccacattta 
      tatggaagg tatttacga agagctaga cgacagttt 
      tgacataaa tgaaatggg cacgtacag cccagcaca 
      gatgatgtt ggagttcga aagctggag tcttttttc 
      ttgtgaaaa agctctaaa gacacaagg attacaaaa 
      gttgctggg ttatcccgc agattcttt cgaccttat 
      tatattcga tgttacgac tggttcttg cttctaccc 
      cttaatcca gaaattcaa ctaccctcc aagtattgc 
      ggtcactgc gtttttgct accaacata cgttgcgca 
      taattttgg caatcactt atatccctc atatattcc 
      ttggtatgg gctccagta cacatcaga aatgagttt 
      tgtggaaag agtttggaa ctttatatt cacttatgc 
      cgacgtggt taggagaaa aataagttt gtatcaaaa 
      ggaacacag tatggccaa agaaatttt tggcgaaaa 
      tatcccgtc gatggaaga acttgaacg gcacatatc 
      tttagtttt ggctaacac ggatccgat tctggattt 
      tcctcaacc tgtcccacc aaacattat cccggttgg 
      aggcctcca tacaaggaa gagtaaaga tttacctca 
      agacattct aacagtatt agacaatgc caagcatgg 
      aattatagt cttttcatt aggttcaaa tctacgttc 
      agataaatt aaataaaca aactcaaaa cgcattgct 
      ggaagcttt tagtaaaat tcaagagac tgtgatttg 
      gaaatttga aagtgatat agaaaattt acctaaaaa 
      tgtcatcgt taggaagtg gttgccgca gaatgatat 
      tctaggaca ccccaacgt gaaattatt tattggaca 
      tggtggtgc tttaagcac acaagaggc attatacca 
      cggtgtccc catgatttg tgttccttt cattgttga 
      ccaacatat taacacgcg gataattgt taacaaaaa 
      cctgggaat tcatctcga ttttaagaa aataaccgc 
      tggttatgt acttcaact gcttagaga agttttgga 
      taaccccaa gtacacgga aaacatgaa aaagatttc 
      aaacatttt cagagaccg tctagagac tcctttaga 
      aagaggcgt attttgggt ggaatatgt tttacggca 
      tggtggagc gcaattctt aacaacccc agccagaga 
      tttctcgta ttttaaagc atgcagtct ggatgttat 
      tgcattcct ttttgcaat tgccacggt tatagtaat 
      tattgtttg taaaatgtt tgcgttaat aatgaaagc 
      atgtagtag caagaaaaa aattaaagt aaattaagt 
      taagtaaaa atttgaata ttgtcaaca taaatttat 
      tgtagaaaa aagaataaa atatattgg acaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY