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Gene information
General information
TC008671 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid49: 7226896..7229601
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008671
    iBeetle screen iB_01385
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 8 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008671 RA
      MFLTRSEYD RGVNTFSPE GRLFQVEYA IEAIKLGST 
      AIGICTSEG VVLAVEKRI TSPLMEPTT IEKIVEVDK 
      HIGCAVSGL MADSRTMID RARVECQNH WFVYNENMS 
      VESCAQAVS NLAIQFGDS DDDGAAMSR PFGVAILFA 
      GIDEKGPQL YHMDPSGTF VQFDAKAIG SGSEGAQQS 
      LQEVYHRSM TLSEATTAA LTILKQVME EKLNSSNVE 
      VMTMTPGAL FHMFTKEQV EEVLLNEKL IDQLQEAQK 
      IAVC
    • >TC008671 RB
      MFLTRSEYD RGVNTFSPE GRLFQVEYA IEAIKLGST 
      AIGICTSEG VVLAVEKRI TSPLMEPTT IEKIVEVDK 
      HIGCAVSGL MADSRTMID RARVECQNH WFVYNENMS 
      VESCAQAVS NLAIQFGDS DDDGAAMSR PFGVAILFA 
      GIDEKGPQL YHMDPSGTF VQFDAKAIG SGSEGAQQS 
      LQEVYHRSM TLSEATTAA LTILKQVME EKLNSSNVE 
      VMTMTPGAL FHMFTKEQV EEVISKIS
    CDS sequence
    • >TC008671 RA
      atgttcctc acaagaagt gaatatgac cgaggtgtg 
      aacactttt tcacctgaa ggtcgcctt ttccaagtg 
      gaatatgct atcgaagct atcaaattg ggttccacc 
      gctattgga atatgcact agtgagggg gttgtcttg 
      gctgtagag aagcgcata acttccccc ttaatggag 
      cccacaaca atagaaaaa attgtagaa gttgataaa 
      catattggc tgtgccgtg tctggcctt atggcagac 
      tctagaaca atgattgat agggcacgt gtggaatgc 
      caaaaccat tggtttgtc tacaacgaa aacatgagt 
      gtggaatct tgtgcccaa gctgtgtcg aatctagcg 
      attcagttt ggcgattct gacgatgat ggtgcagca 
      atgtcaagg ccatttggt gttgccatt ttgtttgct 
      gggattgac gaaaagggg ccccagttg taccatatg 
      gatccctct ggtacattc gttcagttt gatgccaag 
      gcgattgga tctggttca gaaggggct cagcaaagc 
      ttgcaggag gtctaccac cgcagtatg acactaagt 
      gaagcaaca acagcggca ctaacaatt cttaagcaa 
      gtgatggaa gagaaattg aattcttca aatgttgaa 
      gtcatgaca atgactcct ggtgcactg tttcatatg 
      tttactaag gaacaggtg gaggaggtg ttgctgaat 
      gagaagctt atcgatcag ctgcaagaa gctcaaaaa 
      attgctgtc tgttag
    • >TC008671 RB
      atgttcctc acaagaagt gaatatgac cgaggtgtg 
      aacactttt tcacctgaa ggtcgcctt ttccaagtg 
      gaatatgct atcgaagct atcaaattg ggttccacc 
      gctattgga atatgcact agtgagggg gttgtcttg 
      gctgtagag aagcgcata acttccccc ttaatggag 
      cccacaaca atagaaaaa attgtagaa gttgataaa 
      catattggc tgtgccgtg tctggcctt atggcagac 
      tctagaaca atgattgat agggcacgt gtggaatgc 
      caaaaccat tggtttgtc tacaacgaa aacatgagt 
      gtggaatct tgtgcccaa gctgtgtcg aatctagcg 
      attcagttt ggcgattct gacgatgat ggtgcagca 
      atgtcaagg ccatttggt gttgccatt ttgtttgct 
      gggattgac gaaaagggg ccccagttg taccatatg 
      gatccctct ggtacattc gttcagttt gatgccaag 
      gcgattgga tctggttca gaaggggct cagcaaagc 
      ttgcaggag gtctaccac cgcagtatg acactaagt 
      gaagcaaca acagcggca ctaacaatt cttaagcaa 
      gtgatggaa gagaaattg aattcttca aatgttgaa 
      gtcatgaca atgactcct ggtgcactg tttcatatg 
      tttactaag gaacaggtg gaggaggta atttctaaa 
      atatcataa
    mRNA sequence
    • >TC008671 RA
      gtgttttgg tttgtcatc gtcggcttt ttcctgaat 
      ttagaaaaa tttgaatta atccaatta taaagatgt 
      tcctcacaa gaagtgaat atgaccgag gtgtgaaca 
      ctttttcac ctgaaggtc gccttttcc aagtggaat 
      atgctatcg aagctatca aattgggtt ccaccgcta 
      ttggaatat gcactagtg agggggttg tcttggctg 
      tagagaagc gcataactt cccccttaa tggagccca 
      caacaatag aaaaaattg tagaagttg ataaacata 
      ttggctgtg ccgtgtctg gccttatgg cagactcta 
      gaacaatga ttgataggg cacgtgtgg aatgccaaa 
      accattggt ttgtctaca acgaaaaca tgagtgtgg 
      aatcttgtg cccaagctg tgtcgaatc tagcgattc 
      agtttggcg attctgacg atgatggtg cagcaatgt 
      caaggccat ttggtgttg ccattttgt ttgctggga 
      ttgacgaaa aggggcccc agttgtacc atatggatc 
      cctctggta cattcgttc agtttgatg ccaaggcga 
      ttggatctg gttcagaag gggctcagc aaagcttgc 
      aggaggtct accaccgca gtatgacac taagtgaag 
      caacaacag cggcactaa caattctta agcaagtga 
      tggaagaga aattgaatt cttcaaatg ttgaagtca 
      tgacaatga ctcctggtg cactgtttc atatgttta 
      ctaaggaac aggtggagg aggtgttgc tgaatgaga 
      agcttatcg atcagctgc aagaagctc aaaaaattg 
      ctgtctgtt agttttcaa tgccattgt tttaatttt 
      tttctgttt taatatctt cttaaatgt aattcgatg 
      atttgtacg ttgttaata aacacttaa ttattga
    • >TC008671 RB
      gtgttttgg tttgtcatc gtcggcttt ttcctgaat 
      ttagaaaaa tttgaatta atccaatta taaagatgt 
      tcctcacaa gaagtgaat atgaccgag gtgtgaaca 
      ctttttcac ctgaaggtc gccttttcc aagtggaat 
      atgctatcg aagctatca aattgggtt ccaccgcta 
      ttggaatat gcactagtg agggggttg tcttggctg 
      tagagaagc gcataactt cccccttaa tggagccca 
      caacaatag aaaaaattg tagaagttg ataaacata 
      ttggctgtg ccgtgtctg gccttatgg cagactcta 
      gaacaatga ttgataggg cacgtgtgg aatgccaaa 
      accattggt ttgtctaca acgaaaaca tgagtgtgg 
      aatcttgtg cccaagctg tgtcgaatc tagcgattc 
      agtttggcg attctgacg atgatggtg cagcaatgt 
      caaggccat ttggtgttg ccattttgt ttgctggga 
      ttgacgaaa aggggcccc agttgtacc atatggatc 
      cctctggta cattcgttc agtttgatg ccaaggcga 
      ttggatctg gttcagaag gggctcagc aaagcttgc 
      aggaggtct accaccgca gtatgacac taagtgaag 
      caacaacag cggcactaa caattctta agcaagtga 
      tggaagaga aattgaatt cttcaaatg ttgaagtca 
      tgacaatga ctcctggtg cactgtttc atatgttta 
      ctaaggaac aggtggagg aggtaattt ctaaaatat 
      cataattta tatcaaagg caggaggtt gctcgtttt 
      tattttaca agctgtttt atttttcta taccagtat 
      aactacatt tttactgtt tttccatca aaacgatgt 
      tctctgcta acaacatat ttaaatatc gttattatt 
      cactctact ggaacaata aataattaa gaagaaa