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Gene information
General information
TC033856
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008659,TC008660
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid49: 7085231..7102865
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033856
    iBeetle screen iB_01382
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    iBeetle screen iB_04530
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: 3 - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 3
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva appendages mostlyirregular -
    antenna broader - Penetrance (percentage of animals/eggs on slide): more than 80%
    antenna shortened - Penetrance (percentage of animals/eggs on slide): more than 80%

    mandible shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs femur broader - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs femur shortened - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs tibiotarsus broader - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs pretarsus broader - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs tibiotarsus shortened - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs pretarsus shortened - Penetrance (percentage of animals/eggs on slide): more than 80%
    urogomphi broader - Penetrance (percentage of animals/eggs on slide): more than 80%

    urogomphi shortened - Penetrance (percentage of animals/eggs on slide): more than 80%
    pygopodium broader - Penetrance (percentage of animals/eggs on slide): more than 80%

    pygopodium shortened - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033856 RA
      MKYSVFVCL CLLHSVLAV EIEFGGSNR RESKKLFGG 
      YRITPRFCK PTRKATTSL GGPNICMFN HECSQRNGQ 
      VVGACMDGF LFGACCQLP AGASGELIE SDLHPVTSI 
      SNGVSSSTL PPPNEIMSS GVSQITQTL LNPGVLPTA 
      QDNAMVQIS EDQVKFSTT GVTHTTAPE TMILHGISN 
      SISPSDFKM TTKGSVMED RKTTMRFDE TTVPYSEMP 
      TIHISVSNA PSHYTPSPG YQKPVFRPK PSKPSDTDK 
      YVLVPTISH TARPNKTQE IDSIVNILQ MLNETSTNN 
      PYYGQTSKK PPSTSYVFS ATPTRRPGY SPPTTTSKK 
      PPSTSYVFS TTLKPRRTT TPISTTKRK TKPTKNTNR 
      KTTTIRVTT PYDSSSTPK PAGHSTVAN SPLAFLSTR 
      PPSTSYVYS SIPTRNPAS TVNTHIAGP GFAVTSTPG 
      QFSTFPSPA PTLIVLSPV TEDDVITRR PLDEPEYGS 
      SGSPLYTTP RPPSGSIYS SPRPPYARP SLNSNNKPI 
      NSVTINNHV TQNIYSTSE RPLPTVLIT PKPSISSSL 
      NPLDYNNGA VEVEANPDQ LVNFPPVRN PNLNMSNPV 
      LSDEDITTP AFIEDEALN TKVESFVNK IIQGLQEPF 
      DGLKDVVYN RNTTIADTT TKKPVKKQG TTTKKPASK 
      PTSRPTSLR PVTSRPVTT RPTTRRSTT VRRPSSTVT 
      KRPSTTTKR PKTTKRPQT TTESFIEEE NVLPDSEQY 
      RDQCGIRPL LKTGRIVGG KGATFGEFP WQVLVREST 
      WLGLFTKNK CGGVLISNK YVMTAAHCQ PGFLASLVA 
      VFGEFDISG DLESRRPVS RNVRRVIVH RKYDAATFE 
      NDLALLELE SPVKFDAHI IPICLPRDG EDFTGRMAT 
      VTGWGRLKY GGGVPSVLQ EVQVPIMEN HVCQEMFRT 
      AGHSKVILD SFLCAGYAN GQKDSCEGD SGGPLVLQR 
      PDGRYQLAG TVSHGIKCA APYLPGVYM RTTFVVFRL 
      KMRALIILF TACLLGHKA HAWGGLFNR FSPEMLANM 
      GYGGHGGFI QRTGDEDKG ILEEYTNEG EEEPCYGKP 
      CTANEHCCP GSVCVDVDG VVGSCLFAY GRRVGELCR 
      RDSDCESGL VCAEAEPGV STRVCRPPV HQDKQYSEP 
      CNMSSECDI SRGLCCQLQ RRHRQAPRK VCSYFKDPL 
      VCIGPVASD QIKSNIQHT AGEKRLTGL AGFKRPMH
    CDS sequence
    • >TC033856 RA
      atgaagtat agcgtcttt gtgtgtttg tgtctgttg 
      cattctgta ctggctgtg gaaatcgaa tttggggga 
      agcaaccga agagagtcg aaaaagctg tttggagga 
      tacaggata acgcctcgt ttctgtaaa ccgacacga 
      aaagcaacc acatctctc ggaggccct aatatttgc 
      atgttcaac catgaatgc agccagaga aacggccaa 
      gtggtcgga gcctgcatg gatggcttc ctcttcggc 
      gcttgctgc caattgcca gcaggagcg tcaggagaa 
      ctaatagaa agtgatctc catcctgtc acaagtata 
      agcaatgga gtctcatct tcaacactt ccccctccg 
      aatgagata atgtccagt ggagtgtca caaatcacc 
      cagacgttg cttaatccc ggagtgtta ccaactgca 
      caggacaat gcaatggta caaatcagt gaagatcaa 
      gttaaattc tccacaaca ggagttaca cacaccaca 
      gctccggaa acgatgatc ttacacgga atcagcaac 
      agtattagt cccagtgat tttaaaatg accacgaaa 
      ggctccgta atggaagac agaaagacg acaatgaga 
      ttcgatgaa acaacagtg ccatattca gaaatgcca 
      acaattcat atctctgta tccaacgca ccatcgcat 
      tatacacca tcgccaggg taccagaaa ccagttttc 
      cgaccaaaa ccatcaaaa ccgtccgat actgataag 
      tacgtttta gtaccgacg atatctcac acagctaga 
      ccaaataaa acgcaagaa atcgactcg attgtaaat 
      attttgcaa atgttgaat gaaacttcc accaataac 
      ccgtactat ggacaaact tcgaaaaaa ccaccatcg 
      acgtcctat gtctttagt gcaaccccg accagaaga 
      ccgggttat agtcctcca acaacaacg tcaaagaaa 
      ccaccatcc acttcatat gtctttagt acaacattg 
      aaacccaga aggacaaca acaccaata agtaccacg 
      aaacgaaag actaaaccc acgaaaaat acaaatagg 
      aaaactaca actatcagg gttacaaca ccgtacgat 
      tccagcagt accccgaaa ccagcagga cacagtact 
      gtcgctaat tcgcctctt gccttcctc tcaactcga 
      cctccatct acctcctac gtctacagc agcatccca 
      acacgaaat ccagcgtcg actgtcaac acacacatc 
      gcagggcct ggatttgcc gttacgtca acacctggc 
      cagttttca actttccca agtccggca ccaacctta 
      atagtccta agtccggtg actgaagac gatgtaata 
      acacgacgg ccgttggat gaacccgaa tacggttct 
      tccggatct ccactatac acaacaccg agaccaccg 
      tctggatcc atctactca tcccctaga cctccctac 
      gccagaccc agcttgaac tcaaacaac aaacccatc 
      aacagcgtc acaatcaac aaccacgtc actcaaaac 
      atctacagc accagtgaa cgaccccta cccacggtg 
      ttgatcacc cccaagccc agtatctca agctcttta 
      aatcctctg gattacaac aatggtgca gttgaagtt 
      gaagcaaat ccagaccag ttggtcaat ttcccccca 
      gttcgcaat ccgaacctg aacatgtcc aatccagtc 
      ctcagcgat gaggatata accactccg gcgttcatt 
      gaagacgag gcgttgaac accaaagtt gagtcgttt 
      gtgaataag attattcaa gggttgcag gaaccgttc 
      gatggattg aaggatgtt gtctataat cgaaacaca 
      acaatagct gacactacg acgaaaaaa ccggtcaag 
      aaacaaggt actacaacg aaaaaacca gcgtcgaaa 
      ccaacatca agacccact tcactcaga cctgtgact 
      tcaagacca gtgactacg agaccaacg acgaggaga 
      tcaacaaca gttcgaaga ccgtcttca acagttaca 
      aagcggccg tcaactact acgaagagg ccgaaaact 
      acgaaaagg ccgcaaacg acgacagag agtttcatt 
      gaagaggaa aatgttctc ccggattcg gagcaatac 
      agagatcaa tgtggcata agaccttta ttgaaaacc 
      ggaagaata gtcggtggt aaaggggcg actttcggc 
      gaatttccc tggcaagtg ctcgtgcgt gagtcgacc 
      tggctgggc ctcttcaca aagaataag tgcggcggt 
      gttctaatt agtaacaaa tacgtaatg accgccgcc 
      cattgccag cccggattt ttggcctct ttagtggcc 
      gtcttcggc gaattcgac atctccgga gacctcgag 
      agtcgaaga ccggtcagt aggaacgtt agaagagtt 
      atcgtccac agaaaatac gacgctgcc acgttcgaa 
      aacgacttg gctttgctc gaactggaa tcgccggtc 
      aaattcgac gcacatatc atccccatt tgtctacct 
      cgcgacggt gaagacttc actggcaga atggccacg 
      gtgacggga tggggtcgt ctcaagtac ggcggaggc 
      gttccgtcg gtcctccaa gaagtgcaa gtcccaatt 
      atggaaaac cacgtctgt caggaaatg ttccggaca 
      gcaggacat tccaaagtc atcctggac tcgttcctg 
      tgtgcggga tacgcaaac ggccaaaag gactcctgc 
      gagggcgac tcgggaggc cctctcgtc ctgcagagg 
      cctgatgga aggtatcaa ttagcaggg acggtgtcg 
      catgggatc aaatgtgcg gcgccctat ttgccgggg 
      gtgtacatg aggacgact tttgttgtt ttcagacta 
      aaaatgcgt gctctcatc atactattt acggcttgc 
      cttttggga cataaagca catgcatgg ggaggtctt 
      tttaataga ttttcgccc gaaatgctc gcaaacatg 
      ggatacggg ggacatgga ggttttata cagcgaacg 
      ggcgacgaa gacaaggga attttggaa gagtacacc 
      aacgaaggc gaggaggag ccctgctat gggaaaccg 
      tgcactgcc aacgagcac tgctgcccc ggctctgtt 
      tgtgtcgac gtagatgga gtagtcgga tcgtgttta 
      tttgcttac gggcgcaga gttggtgag ttgtgccga 
      agagacagc gattgcgag tcgggcttg gtgtgtgcg 
      gaagcagag ccaggtgtt tcgacccgt gtttgcaga 
      ccccctgtc caccaagac aagcagtac tccgaaccc 
      tgcaacatg tcgagcgag tgcgacatc tcccgcggg 
      ttgtgttgc cagttgcag agacgccac cgacaggcc 
      ccccggaag gtttgctcg tacttcaag gacccgctg 
      gtgtgcata ggccccgtg gcctccgac cagatcaaa 
      agcaacatc cagcacacg gccggggag aagaggctc 
      acaggactg gccggtttt aagagacct atgcactaa
    mRNA sequence
    • >TC033856 RA
      tcgtcaatg agtgtccaa atccgaaat cgcaacatg 
      cccattagt ttcaattcg gtttagaca ttgactaga 
      atcgccaaa atgaagtat agcgtcttt gtgtgtttg 
      tgtctgttg cattctgta ctggctgtg gaaatcgaa 
      tttggggga agcaaccga agagagtcg aaaaagctg 
      tttggagga tacaggata acgcctcgt ttctgtaaa 
      ccgacacga aaagcaacc acatctctc ggaggccct 
      aatatttgc atgttcaac catgaatgc agccagaga 
      aacggccaa gtggtcgga gcctgcatg gatggcttc 
      ctcttcggc gcttgctgc caattgcca gcaggagcg 
      tcaggagaa ctaatagaa agtgatctc catcctgtc 
      acaagtata agcaatgga gtctcatct tcaacactt 
      ccccctccg aatgagata atgtccagt ggagtgtca 
      caaatcacc cagacgttg cttaatccc ggagtgtta 
      ccaactgca caggacaat gcaatggta caaatcagt 
      gaagatcaa gttaaattc tccacaaca ggagttaca 
      cacaccaca gctccggaa acgatgatc ttacacgga 
      atcagcaac agtattagt cccagtgat tttaaaatg 
      accacgaaa ggctccgta atggaagac agaaagacg 
      acaatgaga ttcgatgaa acaacagtg ccatattca 
      gaaatgcca acaattcat atctctgta tccaacgca 
      ccatcgcat tatacacca tcgccaggg taccagaaa 
      ccagttttc cgaccaaaa ccatcaaaa ccgtccgat 
      actgataag tacgtttta gtaccgacg atatctcac 
      acagctaga ccaaataaa acgcaagaa atcgactcg 
      attgtaaat attttgcaa atgttgaat gaaacttcc 
      accaataac ccgtactat ggacaaact tcgaaaaaa 
      ccaccatcg acgtcctat gtctttagt gcaaccccg 
      accagaaga ccgggttat agtcctcca acaacaacg 
      tcaaagaaa ccaccatcc acttcatat gtctttagt 
      acaacattg aaacccaga aggacaaca acaccaata 
      agtaccacg aaacgaaag actaaaccc acgaaaaat 
      acaaatagg aaaactaca actatcagg gttacaaca 
      ccgtacgat tccagcagt accccgaaa ccagcagga 
      cacagtact gtcgctaat tcgcctctt gccttcctc 
      tcaactcga cctccatct acctcctac gtctacagc 
      agcatccca acacgaaat ccagcgtcg actgtcaac 
      acacacatc gcagggcct ggatttgcc gttacgtca 
      acacctggc cagttttca actttccca agtccggca 
      ccaacctta atagtccta agtccggtg actgaagac 
      gatgtaata acacgacgg ccgttggat gaacccgaa 
      tacggttct tccggatct ccactatac acaacaccg 
      agaccaccg tctggatcc atctactca tcccctaga 
      cctccctac gccagaccc agcttgaac tcaaacaac 
      aaacccatc aacagcgtc acaatcaac aaccacgtc 
      actcaaaac atctacagc accagtgaa cgaccccta 
      cccacggtg ttgatcacc cccaagccc agtatctca 
      agctcttta aatcctctg gattacaac aatggtgca 
      gttgaagtt gaagcaaat ccagaccag ttggtcaat 
      ttcccccca gttcgcaat ccgaacctg aacatgtcc 
      aatccagtc ctcagcgat gaggatata accactccg 
      gcgttcatt gaagacgag gcgttgaac accaaagtt 
      gagtcgttt gtgaataag attattcaa gggttgcag 
      gaaccgttc gatggattg aaggatgtt gtctataat 
      cgaaacaca acaatagct gacactacg acgaaaaaa 
      ccggtcaag aaacaaggt actacaacg aaaaaacca 
      gcgtcgaaa ccaacatca agacccact tcactcaga 
      cctgtgact tcaagacca gtgactacg agaccaacg 
      acgaggaga tcaacaaca gttcgaaga ccgtcttca 
      acagttaca aagcggccg tcaactact acgaagagg 
      ccgaaaact acgaaaagg ccgcaaacg acgacagag 
      agtttcatt gaagaggaa aatgttctc ccggattcg 
      gagcaatac agagatcaa tgtggcata agaccttta 
      ttgaaaacc ggaagaata gtcggtggt aaaggggcg 
      actttcggc gaatttccc tggcaagtg ctcgtgcgt 
      gagtcgacc tggctgggc ctcttcaca aagaataag 
      tgcggcggt gttctaatt agtaacaaa tacgtaatg 
      accgccgcc cattgccag cccggattt ttggcctct 
      ttagtggcc gtcttcggc gaattcgac atctccgga 
      gacctcgag agtcgaaga ccggtcagt aggaacgtt 
      agaagagtt atcgtccac agaaaatac gacgctgcc 
      acgttcgaa aacgacttg gctttgctc gaactggaa 
      tcgccggtc aaattcgac gcacatatc atccccatt 
      tgtctacct cgcgacggt gaagacttc actggcaga 
      atggccacg gtgacggga tggggtcgt ctcaagtac 
      ggcggaggc gttccgtcg gtcctccaa gaagtgcaa 
      gtcccaatt atggaaaac cacgtctgt caggaaatg 
      ttccggaca gcaggacat tccaaagtc atcctggac 
      tcgttcctg tgtgcggga tacgcaaac ggccaaaag 
      gactcctgc gagggcgac tcgggaggc cctctcgtc 
      ctgcagagg cctgatgga aggtatcaa ttagcaggg 
      acggtgtcg catgggatc aaatgtgcg gcgccctat 
      ttgccgggg gtgtacatg aggacgact tttgttgtt 
      ttcagacta aaaatgcgt gctctcatc atactattt 
      acggcttgc cttttggga cataaagca catgcatgg 
      ggaggtctt tttaataga ttttcgccc gaaatgctc 
      gcaaacatg ggatacggg ggacatgga ggttttata 
      cagcgaacg ggcgacgaa gacaaggga attttggaa 
      gagtacacc aacgaaggc gaggaggag ccctgctat 
      gggaaaccg tgcactgcc aacgagcac tgctgcccc 
      ggctctgtt tgtgtcgac gtagatgga gtagtcgga 
      tcgtgttta tttgcttac gggcgcaga gttggtgag 
      ttgtgccga agagacagc gattgcgag tcgggcttg 
      gtgtgtgcg gaagcagag ccaggtgtt tcgacccgt 
      gtttgcaga ccccctgtc caccaagac aagcagtac 
      tccgaaccc tgcaacatg tcgagcgag tgcgacatc 
      tcccgcggg ttgtgttgc cagttgcag agacgccac 
      cgacaggcc ccccggaag gtttgctcg tacttcaag 
      gacccgctg gtgtgcata ggccccgtg gcctccgac 
      cagatcaaa agcaacatc cagcacacg gccggggag 
      aagaggctc acaggactg gccggtttt aagagacct 
      atgcactaa atttgtaac aaatcatgt acatttgat 
      cgttacttt ggaagttac gtcgtacgg ttacatcga 
      ttaggtttt tttgttgta cactactac ggttttatt 
      gatttgagg aattctagt gtattagtt cgagccctc 
      caatagaag cgtacaacg taagttctt gagcgcatt 
      aacatccgt aagcgtatt gatttcaat tttacatat 
      tggctgagg tatttatag tgtttaagg tgttttcaa 
      tcaaatttt aaatctggc ctagattta gtattgaga 
      tgataaaaa atacatatc gtagtactt a