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Gene information
General information
TC033856
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008659,TC008660 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 13752578..13770212
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033856
    iBeetle screen iB_01382

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    iBeetle screen iB_04530

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: 3 - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 3
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     larva appendages mostlyirregular -
    antenna broader - Penetrance (percentage of animals/eggs on slide): more than 80%
    antenna shortened - Penetrance (percentage of animals/eggs on slide): more than 80%

    mandible shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs femur broader - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs femur shortened - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs tibiotarsus broader - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs pretarsus broader - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs tibiotarsus shortened - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs pretarsus shortened - Penetrance (percentage of animals/eggs on slide): more than 80%
    urogomphi broader - Penetrance (percentage of animals/eggs on slide): more than 80%

    urogomphi shortened - Penetrance (percentage of animals/eggs on slide): more than 80%
    pygopodium broader - Penetrance (percentage of animals/eggs on slide): more than 80%

    pygopodium shortened - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033856 RA
      MKYSVFVCL CLLHSVLAV EIEFGGSNR RESKKLFGG 
YRITPRFCK PTRKATTSL GGPNICMFN HECSQRNGQ 
VVGACMDGF LFGACCQLP AGASGELIE SDLHPVTSI 
SNGVSSSTL PPPNEIMSS GVSQITQTL LNPGVLPTA 
QDNAMVQIS EDQVKFSTT GVTHTTAPE TMILHGISN 
SISPSDFKM TTKGSVMED RKTTMRFDE TTVPYSEMP 
TIHISVSNA PSHYTPSPG YQKPVFRPK PSKPSDTDK 
YVLVPTISH TARPNKTQE IDSIVNILQ MLNETSTNN 
PYYGQTSKK PPSTSYVFS ATPTRRPGY SPPTTTSKK 
PPSTSYVFS TTLKPRRTT TPISTTKRK TKPTKNTNR 
KTTTIRVTT PYDSSSTPK PAGHSTVAN SPLAFLSTR 
PPSTSYVYS SIPTRNPAS TVNTHIAGP GFAVTSTPG 
QFSTFPSPA PTLIVLSPV TEDDVITRR PLDEPEYGS 
SGSPLYTTP RPPSGSIYS SPRPPYARP SLNSNNKPI 
NSVTINNHV TQNIYSTSE RPLPTVLIT PKPSISSSL 
NPLDYNNGA VEVEANPDQ LVNFPPVRN PNLNMSNPV 
LSDEDITTP AFIEDEALN TKVESFVNK IIQGLQEPF 
DGLKDVVYN RNTTIADTT TKKPVKKQG TTTKKPASK 
PTSRPTSLR PVTSRPVTT RPTTRRSTT VRRPSSTVT 
KRPSTTTKR PKTTKRPQT TTESFIEEE NVLPDSEQY 
RDQCGIRPL LKTGRIVGG KGATFGEFP WQVLVREST 
WLGLFTKNK CGGVLISNK YVMTAAHCQ PGFLASLVA 
VFGEFDISG DLESRRPVS RNVRRVIVH RKYDAATFE 
NDLALLELE SPVKFDAHI IPICLPRDG EDFTGRMAT 
VTGWGRLKY GGGVPSVLQ EVQVPIMEN HVCQEMFRT 
AGHSKVILD SFLCAGYAN GQKDSCEGD SGGPLVLQR 
PDGRYQLAG TVSHGIKCA APYLPGVYM RTTFVVFRL 
KMRALIILF TACLLGHKA HAWGGLFNR FSPEMLANM 
GYGGHGGFI QRTGDEDKG ILEEYTNEG EEEPCYGKP 
CTANEHCCP GSVCVDVDG VVGSCLFAY GRRVGELCR 
RDSDCESGL VCAEAEPGV STRVCRPPV HQDKQYSEP 
CNMSSECDI SRGLCCQLQ RRHRQAPRK VCSYFKDPL 
VCIGPVASD QIKSNIQHT AGEKRLTGL AGFKRPMH
    CDS sequence
    • >TC033856 RA
      atgaagtat agcgtcttt gtgtgtttg tgtctgttg 
cattctgta ctggctgtg gaaatcgaa tttggggga 
agcaaccga agagagtcg aaaaagctg tttggagga 
tacaggata acgcctcgt ttctgtaaa ccgacacga 
aaagcaacc acatctctc ggaggccct aatatttgc 
atgttcaac catgaatgc agccagaga aacggccaa 
gtggtcgga gcctgcatg gatggcttc ctcttcggc 
gcttgctgc caattgcca gcaggagcg tcaggagaa 
ctaatagaa agtgatctc catcctgtc acaagtata 
agcaatgga gtctcatct tcaacactt ccccctccg 
aatgagata atgtccagt ggagtgtca caaatcacc 
cagacgttg cttaatccc ggagtgtta ccaactgca 
caggacaat gcaatggta caaatcagt gaagatcaa 
gttaaattc tccacaaca ggagttaca cacaccaca 
gctccggaa acgatgatc ttacacgga atcagcaac 
agtattagt cccagtgat tttaaaatg accacgaaa 
ggctccgta atggaagac agaaagacg acaatgaga 
ttcgatgaa acaacagtg ccatattca gaaatgcca 
acaattcat atctctgta tccaacgca ccatcgcat 
tatacacca tcgccaggg taccagaaa ccagttttc 
cgaccaaaa ccatcaaaa ccgtccgat actgataag 
tacgtttta gtaccgacg atatctcac acagctaga 
ccaaataaa acgcaagaa atcgactcg attgtaaat 
attttgcaa atgttgaat gaaacttcc accaataac 
ccgtactat ggacaaact tcgaaaaaa ccaccatcg 
acgtcctat gtctttagt gcaaccccg accagaaga 
ccgggttat agtcctcca acaacaacg tcaaagaaa 
ccaccatcc acttcatat gtctttagt acaacattg 
aaacccaga aggacaaca acaccaata agtaccacg 
aaacgaaag actaaaccc acgaaaaat acaaatagg 
aaaactaca actatcagg gttacaaca ccgtacgat 
tccagcagt accccgaaa ccagcagga cacagtact 
gtcgctaat tcgcctctt gccttcctc tcaactcga 
cctccatct acctcctac gtctacagc agcatccca 
acacgaaat ccagcgtcg actgtcaac acacacatc 
gcagggcct ggatttgcc gttacgtca acacctggc 
cagttttca actttccca agtccggca ccaacctta 
atagtccta agtccggtg actgaagac gatgtaata 
acacgacgg ccgttggat gaacccgaa tacggttct 
tccggatct ccactatac acaacaccg agaccaccg 
tctggatcc atctactca tcccctaga cctccctac 
gccagaccc agcttgaac tcaaacaac aaacccatc 
aacagcgtc acaatcaac aaccacgtc actcaaaac 
atctacagc accagtgaa cgaccccta cccacggtg 
ttgatcacc cccaagccc agtatctca agctcttta 
aatcctctg gattacaac aatggtgca gttgaagtt 
gaagcaaat ccagaccag ttggtcaat ttcccccca 
gttcgcaat ccgaacctg aacatgtcc aatccagtc 
ctcagcgat gaggatata accactccg gcgttcatt 
gaagacgag gcgttgaac accaaagtt gagtcgttt 
gtgaataag attattcaa gggttgcag gaaccgttc 
gatggattg aaggatgtt gtctataat cgaaacaca 
acaatagct gacactacg acgaaaaaa ccggtcaag 
aaacaaggt actacaacg aaaaaacca gcgtcgaaa 
ccaacatca agacccact tcactcaga cctgtgact 
tcaagacca gtgactacg agaccaacg acgaggaga 
tcaacaaca gttcgaaga ccgtcttca acagttaca 
aagcggccg tcaactact acgaagagg ccgaaaact 
acgaaaagg ccgcaaacg acgacagag agtttcatt 
gaagaggaa aatgttctc ccggattcg gagcaatac 
agagatcaa tgtggcata agaccttta ttgaaaacc 
ggaagaata gtcggtggt aaaggggcg actttcggc 
gaatttccc tggcaagtg ctcgtgcgt gagtcgacc 
tggctgggc ctcttcaca aagaataag tgcggcggt 
gttctaatt agtaacaaa tacgtaatg accgccgcc 
cattgccag cccggattt ttggcctct ttagtggcc 
gtcttcggc gaattcgac atctccgga gacctcgag 
agtcgaaga ccggtcagt aggaacgtt agaagagtt 
atcgtccac agaaaatac gacgctgcc acgttcgaa 
aacgacttg gctttgctc gaactggaa tcgccggtc 
aaattcgac gcacatatc atccccatt tgtctacct 
cgcgacggt gaagacttc actggcaga atggccacg 
gtgacggga tggggtcgt ctcaagtac ggcggaggc 
gttccgtcg gtcctccaa gaagtgcaa gtcccaatt 
atggaaaac cacgtctgt caggaaatg ttccggaca 
gcaggacat tccaaagtc atcctggac tcgttcctg 
tgtgcggga tacgcaaac ggccaaaag gactcctgc 
gagggcgac tcgggaggc cctctcgtc ctgcagagg 
cctgatgga aggtatcaa ttagcaggg acggtgtcg 
catgggatc aaatgtgcg gcgccctat ttgccgggg 
gtgtacatg aggacgact tttgttgtt ttcagacta 
aaaatgcgt gctctcatc atactattt acggcttgc 
cttttggga cataaagca catgcatgg ggaggtctt 
tttaataga ttttcgccc gaaatgctc gcaaacatg 
ggatacggg ggacatgga ggttttata cagcgaacg 
ggcgacgaa gacaaggga attttggaa gagtacacc 
aacgaaggc gaggaggag ccctgctat gggaaaccg 
tgcactgcc aacgagcac tgctgcccc ggctctgtt 
tgtgtcgac gtagatgga gtagtcgga tcgtgttta 
tttgcttac gggcgcaga gttggtgag ttgtgccga 
agagacagc gattgcgag tcgggcttg gtgtgtgcg 
gaagcagag ccaggtgtt tcgacccgt gtttgcaga 
ccccctgtc caccaagac aagcagtac tccgaaccc 
tgcaacatg tcgagcgag tgcgacatc tcccgcggg 
ttgtgttgc cagttgcag agacgccac cgacaggcc 
ccccggaag gtttgctcg tacttcaag gacccgctg 
gtgtgcata ggccccgtg gcctccgac cagatcaaa 
agcaacatc cagcacacg gccggggag aagaggctc 
acaggactg gccggtttt aagagacct atgcactaa
    mRNA sequence
    • >TC033856 RA
      tcgtcaatg agtgtccaa atccgaaat cgcaacatg 
cccattagt ttcaattcg gtttagaca ttgactaga 
atcgccaaa atgaagtat agcgtcttt gtgtgtttg 
tgtctgttg cattctgta ctggctgtg gaaatcgaa 
tttggggga agcaaccga agagagtcg aaaaagctg 
tttggagga tacaggata acgcctcgt ttctgtaaa 
ccgacacga aaagcaacc acatctctc ggaggccct 
aatatttgc atgttcaac catgaatgc agccagaga 
aacggccaa gtggtcgga gcctgcatg gatggcttc 
ctcttcggc gcttgctgc caattgcca gcaggagcg 
tcaggagaa ctaatagaa agtgatctc catcctgtc 
acaagtata agcaatgga gtctcatct tcaacactt 
ccccctccg aatgagata atgtccagt ggagtgtca 
caaatcacc cagacgttg cttaatccc ggagtgtta 
ccaactgca caggacaat gcaatggta caaatcagt 
gaagatcaa gttaaattc tccacaaca ggagttaca 
cacaccaca gctccggaa acgatgatc ttacacgga 
atcagcaac agtattagt cccagtgat tttaaaatg 
accacgaaa ggctccgta atggaagac agaaagacg 
acaatgaga ttcgatgaa acaacagtg ccatattca 
gaaatgcca acaattcat atctctgta tccaacgca 
ccatcgcat tatacacca tcgccaggg taccagaaa 
ccagttttc cgaccaaaa ccatcaaaa ccgtccgat 
actgataag tacgtttta gtaccgacg atatctcac 
acagctaga ccaaataaa acgcaagaa atcgactcg 
attgtaaat attttgcaa atgttgaat gaaacttcc 
accaataac ccgtactat ggacaaact tcgaaaaaa 
ccaccatcg acgtcctat gtctttagt gcaaccccg 
accagaaga ccgggttat agtcctcca acaacaacg 
tcaaagaaa ccaccatcc acttcatat gtctttagt 
acaacattg aaacccaga aggacaaca acaccaata 
agtaccacg aaacgaaag actaaaccc acgaaaaat 
acaaatagg aaaactaca actatcagg gttacaaca 
ccgtacgat tccagcagt accccgaaa ccagcagga 
cacagtact gtcgctaat tcgcctctt gccttcctc 
tcaactcga cctccatct acctcctac gtctacagc 
agcatccca acacgaaat ccagcgtcg actgtcaac 
acacacatc gcagggcct ggatttgcc gttacgtca 
acacctggc cagttttca actttccca agtccggca 
ccaacctta atagtccta agtccggtg actgaagac 
gatgtaata acacgacgg ccgttggat gaacccgaa 
tacggttct tccggatct ccactatac acaacaccg 
agaccaccg tctggatcc atctactca tcccctaga 
cctccctac gccagaccc agcttgaac tcaaacaac 
aaacccatc aacagcgtc acaatcaac aaccacgtc 
actcaaaac atctacagc accagtgaa cgaccccta 
cccacggtg ttgatcacc cccaagccc agtatctca 
agctcttta aatcctctg gattacaac aatggtgca 
gttgaagtt gaagcaaat ccagaccag ttggtcaat 
ttcccccca gttcgcaat ccgaacctg aacatgtcc 
aatccagtc ctcagcgat gaggatata accactccg 
gcgttcatt gaagacgag gcgttgaac accaaagtt 
gagtcgttt gtgaataag attattcaa gggttgcag 
gaaccgttc gatggattg aaggatgtt gtctataat 
cgaaacaca acaatagct gacactacg acgaaaaaa 
ccggtcaag aaacaaggt actacaacg aaaaaacca 
gcgtcgaaa ccaacatca agacccact tcactcaga 
cctgtgact tcaagacca gtgactacg agaccaacg 
acgaggaga tcaacaaca gttcgaaga ccgtcttca 
acagttaca aagcggccg tcaactact acgaagagg 
ccgaaaact acgaaaagg ccgcaaacg acgacagag 
agtttcatt gaagaggaa aatgttctc ccggattcg 
gagcaatac agagatcaa tgtggcata agaccttta 
ttgaaaacc ggaagaata gtcggtggt aaaggggcg 
actttcggc gaatttccc tggcaagtg ctcgtgcgt 
gagtcgacc tggctgggc ctcttcaca aagaataag 
tgcggcggt gttctaatt agtaacaaa tacgtaatg 
accgccgcc cattgccag cccggattt ttggcctct 
ttagtggcc gtcttcggc gaattcgac atctccgga 
gacctcgag agtcgaaga ccggtcagt aggaacgtt 
agaagagtt atcgtccac agaaaatac gacgctgcc 
acgttcgaa aacgacttg gctttgctc gaactggaa 
tcgccggtc aaattcgac gcacatatc atccccatt 
tgtctacct cgcgacggt gaagacttc actggcaga 
atggccacg gtgacggga tggggtcgt ctcaagtac 
ggcggaggc gttccgtcg gtcctccaa gaagtgcaa 
gtcccaatt atggaaaac cacgtctgt caggaaatg 
ttccggaca gcaggacat tccaaagtc atcctggac 
tcgttcctg tgtgcggga tacgcaaac ggccaaaag 
gactcctgc gagggcgac tcgggaggc cctctcgtc 
ctgcagagg cctgatgga aggtatcaa ttagcaggg 
acggtgtcg catgggatc aaatgtgcg gcgccctat 
ttgccgggg gtgtacatg aggacgact tttgttgtt 
ttcagacta aaaatgcgt gctctcatc atactattt 
acggcttgc cttttggga cataaagca catgcatgg 
ggaggtctt tttaataga ttttcgccc gaaatgctc 
gcaaacatg ggatacggg ggacatgga ggttttata 
cagcgaacg ggcgacgaa gacaaggga attttggaa 
gagtacacc aacgaaggc gaggaggag ccctgctat 
gggaaaccg tgcactgcc aacgagcac tgctgcccc 
ggctctgtt tgtgtcgac gtagatgga gtagtcgga 
tcgtgttta tttgcttac gggcgcaga gttggtgag 
ttgtgccga agagacagc gattgcgag tcgggcttg 
gtgtgtgcg gaagcagag ccaggtgtt tcgacccgt 
gtttgcaga ccccctgtc caccaagac aagcagtac 
tccgaaccc tgcaacatg tcgagcgag tgcgacatc 
tcccgcggg ttgtgttgc cagttgcag agacgccac 
cgacaggcc ccccggaag gtttgctcg tacttcaag 
gacccgctg gtgtgcata ggccccgtg gcctccgac 
cagatcaaa agcaacatc cagcacacg gccggggag 
aagaggctc acaggactg gccggtttt aagagacct 
atgcactaa atttgtaac aaatcatgt acatttgat 
cgttacttt ggaagttac gtcgtacgg ttacatcga 
ttaggtttt tttgttgta cactactac ggttttatt 
gatttgagg aattctagt gtattagtt cgagccctc 
caatagaag cgtacaacg taagttctt gagcgcatt 
aacatccgt aagcgtatt gatttcaat tttacatat 
tggctgagg tatttatag tgtttaagg tgttttcaa 
tcaaatttt aaatctggc ctagattta gtattgaga 
tgataaaaa atacatatc gtagtactt a
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