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Gene information
General information
TC008653 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 13701159..13710160
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008653
    iBeetle screen iB_01381

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature random pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008653 RA
      MNKIHSRIL ALVLSIFLI HAADPPVQR PSGAFIQYL 
      PKSNQTDDF QETIEQNTE KLKLFHDYL ISIKNESPE 
      NSGDKDTSN HHAINRFLQ RERKTDYEK LLEKDFIRP 
      LLKLYEQHI HQEPDYTIG NSSTLSRIS KEDHVEDFK 
      VLSKTDLDV LIHGGETYQ QNHNQDTDQ HIARRDDTV 
      DGSHDEHIG EILPPGYQP PKMHRLSPV RRFYYQPSA 
      RSRKSYDEG FKTFRKPVI FPGERFRPE YEPSGFSSE 
      GFESEYRPE YDINTEESR NMFTRPTRL GLPPNEVPG 
      FRLPRRLRG FPENFPPTA REPYWQTPW ASSRRPRVI 
      FPSDLVAFR EPNGNQEEP DWLAGDNNL QDIQEQDTR 
      DRGTQLGCA SGVTCEFFL TCWLSGGLL ESPCDGLLR 
      GCCQRGASK AGQSANLAI GTLEAPRES PKAGLVDSD 
      YRCGISTNR QQAQRRIVG GEEAGFGTF PWQAYIRIG 
      SSRCGGSLV SRRHVVTAG HCVARATPR QVHVTLGDY 
      VINSAVEPL PAYTFGVSQ IQVHPFFKF TPQADRFDV 
      AVLRLDRTA HQLPHITPI CLPPRGESF LGEVGVAAG 
      WGALSPGSR LRPQTLQAV QVPVIDNRV CERWHRSKG 
      IGVTIYDEM MCAGYKNGG RDSCQGDSG GPLMLQKQG 
      RWFLIGIVS AGYSCAQPG QPGIYHRVA HTVDWITRA 
      IGV
    • >TC008653 RB
      MNKIHSRIL ALVLSIFLI HAADPPVQR PSGAFIQYL 
      PKSNQTDDF QETIEQNTE KLKLFHDYL ISIKNESPE 
      NSGDKDTSN HHAINRFLQ RERKTDYEK LLEKDFIRP 
      LLKLYEQHI HQEPDYTIG NSSTLSRIS KEDHVEDFK 
      VLSKTDLDV LIHGGETYQ QNHNQDTDQ HIARRDDTV 
      DGSHDEHIG EILPPGYQP PKMHRLSPV RRFYYQPSA 
      RSRKSYDEG FKTFRKPVI FPGERFRPE YEPSGFSSE 
      GFESEYRPE YDINTEESR NMFTRPTRL GLPPNEVPG 
      FRLPRRLRG FPENFPPTA REPYWQTPW ASSRRPRVI 
      FPSDLVAFR EPNGNQEEP DWLAGDNNL QDIQEQDTR 
      DRGCGISTN RQQAQRRIV GGEEAGFGT FPWQAYIRI 
      GSSRCGGSL VSRRHVVTA GHCVARATP RQVHVTLGD 
      YVINSAVEP LPAYTFGVS QIQVHPFFK FTPQADRFD 
      VAVLRLDRT AHQLPHITP ICLPPRGES FLGEVGVAA 
      GWGALSPGS RLRPQTLQA VQVPVIDNR VCERWHRSK 
      GIGVTIYDE MMCAGYKNG GRDSCQGDS GGPLMLQKQ 
      GRWFLIGIV SAGYSCAQP GQPGIYHRV AHTVDWITR 
      AIGV
    CDS sequence
    • >TC008653 RA
      atgaacaaa atacattcg cgcattttg gcactggtt 
      ttgagcata tttctgata cacgcagct gacccgcca 
      gtccagaga ccgtcaggg gccttcata cagtacctc 
      cccaagagc aaccaaacc gacgacttt caggaaacc 
      atcgagcaa aacacggaa aaactcaaa ctctttcac 
      gattatttg attagcatc aaaaatgag tcacccgaa 
      aattccggt gataaagac acttcgaat catcacgcc 
      ataaaccga tttctccaa agagaaaga aaaacggac 
      tacgagaag ttgctcgag aaggatttc ataagaccc 
      ttattaaag ctttacgag cagcacatc catcaagag 
      cccgattac acaatagga aattcctcc acgttatct 
      cgaatttcc aaagaagat cacgtcgag gatttcaaa 
      gtgttgtcg aaaaccgac ttggacgtg ctgatccat 
      gggggtgaa acctaccag cagaaccac aaccaagat 
      accgatcaa catatcgcc cgaagggat gatacagtt 
      gatgggagt cacgatgaa cacattggg gagattttg 
      ccccctggg taccagccc cccaagatg catcgactc 
      agccccgtg aggcgattc tactaccaa ccctcggcc 
      agaagtcgg aagtcgtac gatgagggt tttaagacg 
      ttccgcaag ccggtgatt tttccgggg gagcgcttc 
      cgaccggag tatgaacct agcgggttc tcatcggaa 
      ggattcgag tctgagtat agaccggaa tatgatatt 
      aacaccgag gaaagtcgg aacatgttc acgagacct 
      acgaggttg ggtttacca cctaacgaa gtacctggc 
      ttccgatta ccgagacgt ttgcggggc ttcccggag 
      aattttcct ccaacggct agagaaccg tactggcag 
      accccttgg gctagttct agacgacca cgagtgatc 
      tttccttcg gatttagtc gcgtttaga gagccaaac 
      ggtaatcag gaagaacct gactggtta gctggagat 
      aacaacttg caagacatc caggaacag gatactcga 
      gatagaggg acacagttg gggtgcgct tccggtgtg 
      acgtgcgag tttttccta acttgttgg ctgtcgggg 
      ggcctgctg gagtccccc tgcgacggc ctgttgcgg 
      ggctgctgc cagaggggg gcctctaag gccggtcaa 
      agcgcaaac ctggcgatc ggtaccctc gaggcaccc 
      cgggaatcg cccaaggca ggcctggtc gactccgat 
      tatcgatgc gggatttcg acgaatcgg cagcaggct 
      cagcggagg attgtcgga ggcgaggaa gcaggattt 
      gggactttt ccttggcag gcttacatc cgaatcgga 
      tcctccaga tgcggaggt tctctagtc agcaggcgc 
      cacgtcgtt acagcaggt cattgtgta gcacgagcc 
      acccccaga caggtgcat gtcaccctt ggagattat 
      gttattaac tcagcagtc gagccgcta ccagcttac 
      acatttggg gtttctcag atacaggtg catcccttt 
      ttcaagttc accccccag gccgaccgc ttcgacgtg 
      gccgtcctt cgcctggac cgcaccgct caccagctg 
      ccccacatc acccccatc tgccttccc ccgcgcggg 
      gagagcttc ctgggggag gtgggcgtc gcggcgggc 
      tggggcgcc ctgagcccc ggctccaga ctgagaccg 
      cagacgctg caagccgtg caagtgcca gtgatagac 
      aatcgcgtg tgcgagcgg tggcacagg tcgaagggc 
      atcggggtg acgatctac gacgagatg atgtgcgcg 
      gggtacaag aacggcggc agggacagc tgccaaggc 
      gactccggg ggccccctc atgctccag aagcagggg 
      cggtggttc ctcatcggg atcgtctcg gcggggtac 
      agctgcgcc cagccgggc cagcccggg atctaccac 
      cgggtggcg cataccgtt gactggatc acaagggcc 
      atcggtgtt tag
    • >TC008653 RB
      atgaacaaa atacattcg cgcattttg gcactggtt 
      ttgagcata tttctgata cacgcagct gacccgcca 
      gtccagaga ccgtcaggg gccttcata cagtacctc 
      cccaagagc aaccaaacc gacgacttt caggaaacc 
      atcgagcaa aacacggaa aaactcaaa ctctttcac 
      gattatttg attagcatc aaaaatgag tcacccgaa 
      aattccggt gataaagac acttcgaat catcacgcc 
      ataaaccga tttctccaa agagaaaga aaaacggac 
      tacgagaag ttgctcgag aaggatttc ataagaccc 
      ttattaaag ctttacgag cagcacatc catcaagag 
      cccgattac acaatagga aattcctcc acgttatct 
      cgaatttcc aaagaagat cacgtcgag gatttcaaa 
      gtgttgtcg aaaaccgac ttggacgtg ctgatccat 
      gggggtgaa acctaccag cagaaccac aaccaagat 
      accgatcaa catatcgcc cgaagggat gatacagtt 
      gatgggagt cacgatgaa cacattggg gagattttg 
      ccccctggg taccagccc cccaagatg catcgactc 
      agccccgtg aggcgattc tactaccaa ccctcggcc 
      agaagtcgg aagtcgtac gatgagggt tttaagacg 
      ttccgcaag ccggtgatt tttccgggg gagcgcttc 
      cgaccggag tatgaacct agcgggttc tcatcggaa 
      ggattcgag tctgagtat agaccggaa tatgatatt 
      aacaccgag gaaagtcgg aacatgttc acgagacct 
      acgaggttg ggtttacca cctaacgaa gtacctggc 
      ttccgatta ccgagacgt ttgcggggc ttcccggag 
      aattttcct ccaacggct agagaaccg tactggcag 
      accccttgg gctagttct agacgacca cgagtgatc 
      tttccttcg gatttagtc gcgtttaga gagccaaac 
      ggtaatcag gaagaacct gactggtta gctggagat 
      aacaacttg caagacatc caggaacag gatactcga 
      gatagagga tgcgggatt tcgacgaat cggcagcag 
      gctcagcgg aggattgtc ggaggcgag gaagcagga 
      tttgggact tttccttgg caggcttac atccgaatc 
      ggatcctcc agatgcgga ggttctcta gtcagcagg 
      cgccacgtc gttacagca ggtcattgt gtagcacga 
      gccaccccc agacaggtg catgtcacc cttggagat 
      tatgttatt aactcagca gtcgagccg ctaccagct 
      tacacattt ggggtttct cagatacag gtgcatccc 
      tttttcaag ttcaccccc caggccgac cgcttcgac 
      gtggccgtc cttcgcctg gaccgcacc gctcaccag 
      ctgccccac atcaccccc atctgcctt cccccgcgc 
      ggggagagc ttcctgggg gaggtgggc gtcgcggcg 
      ggctggggc gccctgagc cccggctcc agactgaga 
      ccgcagacg ctgcaagcc gtgcaagtg ccagtgata 
      gacaatcgc gtgtgcgag cggtggcac aggtcgaag 
      ggcatcggg gtgacgatc tacgacgag atgatgtgc 
      gcggggtac aagaacggc ggcagggac agctgccaa 
      ggcgactcc gggggcccc ctcatgctc cagaagcag 
      gggcggtgg ttcctcatc gggatcgtc tcggcgggg 
      tacagctgc gcccagccg ggccagccc gggatctac 
      caccgggtg gcgcatacc gttgactgg atcacaagg 
      gccatcggt gtttag
    mRNA sequence
    • >TC008653 RA
      ctccataaa taattcttc ggcgtttag caaatttgt 
      cccaatcag cgtttgcaa tttttctga agcgccatc 
      atttattat gcacttttg aagagtcgt gaatctttt 
      acttgcatg ttgtttgta tggggagcg aagtctctt 
      gaagtgaaa gtggtgagt gtgtgaaag ttaactctc 
      tagcatgga cgtctttgt tgcttcagt cggccccta 
      gtgttcatc ggtgcctgt gatgtgttc aagtgattt 
      gtgttttgt tttgtaacc gaacaaaac cgattaacc 
      aaacaatac tcgaaaaat gcattaagt tcaaacact 
      tgcaagaaa aggaaagaa ctgtgatat tagattatt 
      agaagcgta ggatgataa tttaggata agggacagg 
      gcgtttgac agagcgagg gttgaaatt aaatggaac 
      agagtcaga atgaacaaa atacattcg cgcattttg 
      gcactggtt ttgagcata tttctgata cacgcagct 
      gacccgcca gtccagaga ccgtcaggg gccttcata 
      cagtacctc cccaagagc aaccaaacc gacgacttt 
      caggaaacc atcgagcaa aacacggaa aaactcaaa 
      ctctttcac gattatttg attagcatc aaaaatgag 
      tcacccgaa aattccggt gataaagac acttcgaat 
      catcacgcc ataaaccga tttctccaa agagaaaga 
      aaaacggac tacgagaag ttgctcgag aaggatttc 
      ataagaccc ttattaaag ctttacgag cagcacatc 
      catcaagag cccgattac acaatagga aattcctcc 
      acgttatct cgaatttcc aaagaagat cacgtcgag 
      gatttcaaa gtgttgtcg aaaaccgac ttggacgtg 
      ctgatccat gggggtgaa acctaccag cagaaccac 
      aaccaagat accgatcaa catatcgcc cgaagggat 
      gatacagtt gatgggagt cacgatgaa cacattggg 
      gagattttg ccccctggg taccagccc cccaagatg 
      catcgactc agccccgtg aggcgattc tactaccaa 
      ccctcggcc agaagtcgg aagtcgtac gatgagggt 
      tttaagacg ttccgcaag ccggtgatt tttccgggg 
      gagcgcttc cgaccggag tatgaacct agcgggttc 
      tcatcggaa ggattcgag tctgagtat agaccggaa 
      tatgatatt aacaccgag gaaagtcgg aacatgttc 
      acgagacct acgaggttg ggtttacca cctaacgaa 
      gtacctggc ttccgatta ccgagacgt ttgcggggc 
      ttcccggag aattttcct ccaacggct agagaaccg 
      tactggcag accccttgg gctagttct agacgacca 
      cgagtgatc tttccttcg gatttagtc gcgtttaga 
      gagccaaac ggtaatcag gaagaacct gactggtta 
      gctggagat aacaacttg caagacatc caggaacag 
      gatactcga gatagaggg acacagttg gggtgcgct 
      tccggtgtg acgtgcgag tttttccta acttgttgg 
      ctgtcgggg ggcctgctg gagtccccc tgcgacggc 
      ctgttgcgg ggctgctgc cagaggggg gcctctaag 
      gccggtcaa agcgcaaac ctggcgatc ggtaccctc 
      gaggcaccc cgggaatcg cccaaggca ggcctggtc 
      gactccgat tatcgatgc gggatttcg acgaatcgg 
      cagcaggct cagcggagg attgtcgga ggcgaggaa 
      gcaggattt gggactttt ccttggcag gcttacatc 
      cgaatcgga tcctccaga tgcggaggt tctctagtc 
      agcaggcgc cacgtcgtt acagcaggt cattgtgta 
      gcacgagcc acccccaga caggtgcat gtcaccctt 
      ggagattat gttattaac tcagcagtc gagccgcta 
      ccagcttac acatttggg gtttctcag atacaggtg 
      catcccttt ttcaagttc accccccag gccgaccgc 
      ttcgacgtg gccgtcctt cgcctggac cgcaccgct 
      caccagctg ccccacatc acccccatc tgccttccc 
      ccgcgcggg gagagcttc ctgggggag gtgggcgtc 
      gcggcgggc tggggcgcc ctgagcccc ggctccaga 
      ctgagaccg cagacgctg caagccgtg caagtgcca 
      gtgatagac aatcgcgtg tgcgagcgg tggcacagg 
      tcgaagggc atcggggtg acgatctac gacgagatg 
      atgtgcgcg gggtacaag aacggcggc agggacagc 
      tgccaaggc gactccggg ggccccctc atgctccag 
      aagcagggg cggtggttc ctcatcggg atcgtctcg 
      gcggggtac agctgcgcc cagccgggc cagcccggg 
      atctaccac cgggtggcg cataccgtt gactggatc 
      acaagggcc atcggtgtt tagttacct ctagagtcg 
      ctacggaaa tgtaatctg ttcccggaa tcgggggag 
      tgtcgggat gagttccaa ccaaacagt atttcatgt 
      aggtggttg aagcattgc gacaaagtt cataaaacg 
      gctgaaagg acttttaaa acctctttc tgttattag 
      atttccaaa agttgtgct tttacaaaa aacgttaaa 
      aaagtagga caaaaaata atcgtagcg tctatgtta 
      taaataacg ccatttctc agtcatata gtgttgtaa 
      ttcagtgtt aatttattt tatacttaa taagtcaac 
      taccagaac cacaaatct gacaagcaa tgatcattt 
      actcgtatg tacatacag aaatgaaac ttttatttt 
      gctccggtg gaaaagttt taatttctc cttactctt 
      atagccaat aaaaatgta tattttgtg atttgtcaa 
      tcaatacat gagaaagta atttaagta ccctgtaat 
      taactgtaa attaaattt taaaacagg tttagtaaa 
      ttatttaca taaattatt aaattcttg tatatgtac 
      gattataaa actggatgt tatttatgt tattttgtt 
      gtaggatta ttttaagat gatgtactt gaacgatat 
      gactaaata aaatttatt ttgaatt
    • >TC008653 RB
      ctccataaa taattcttc ggcgtttag caaatttgt 
      cccaatcag cgtttgcaa tttttctga agcgccatc 
      atttattat gcacttttg aagagtcgt gaatctttt 
      acttgcatg ttgtttgta tggggagcg aagtctctt 
      gaagtgaaa gtggtgagt gtgtgaaag ttaactctc 
      tagcatgga cgtctttgt tgcttcagt cggccccta 
      gtgttcatc ggtgcctgt gatgtgttc aagtgattt 
      gtgttttgt tttgtaacc gaacaaaac cgattaacc 
      aaacaatac tcgaaaaat gcattaagt tcaaacact 
      tgcaagaaa aggaaagaa ctgtgatat tagattatt 
      agaagcgta ggatgataa tttaggata agggacagg 
      gcgtttgac agagcgagg gttgaaatt aaatggaac 
      agagtcaga atgaacaaa atacattcg cgcattttg 
      gcactggtt ttgagcata tttctgata cacgcagct 
      gacccgcca gtccagaga ccgtcaggg gccttcata 
      cagtacctc cccaagagc aaccaaacc gacgacttt 
      caggaaacc atcgagcaa aacacggaa aaactcaaa 
      ctctttcac gattatttg attagcatc aaaaatgag 
      tcacccgaa aattccggt gataaagac acttcgaat 
      catcacgcc ataaaccga tttctccaa agagaaaga 
      aaaacggac tacgagaag ttgctcgag aaggatttc 
      ataagaccc ttattaaag ctttacgag cagcacatc 
      catcaagag cccgattac acaatagga aattcctcc 
      acgttatct cgaatttcc aaagaagat cacgtcgag 
      gatttcaaa gtgttgtcg aaaaccgac ttggacgtg 
      ctgatccat gggggtgaa acctaccag cagaaccac 
      aaccaagat accgatcaa catatcgcc cgaagggat 
      gatacagtt gatgggagt cacgatgaa cacattggg 
      gagattttg ccccctggg taccagccc cccaagatg 
      catcgactc agccccgtg aggcgattc tactaccaa 
      ccctcggcc agaagtcgg aagtcgtac gatgagggt 
      tttaagacg ttccgcaag ccggtgatt tttccgggg 
      gagcgcttc cgaccggag tatgaacct agcgggttc 
      tcatcggaa ggattcgag tctgagtat agaccggaa 
      tatgatatt aacaccgag gaaagtcgg aacatgttc 
      acgagacct acgaggttg ggtttacca cctaacgaa 
      gtacctggc ttccgatta ccgagacgt ttgcggggc 
      ttcccggag aattttcct ccaacggct agagaaccg 
      tactggcag accccttgg gctagttct agacgacca 
      cgagtgatc tttccttcg gatttagtc gcgtttaga 
      gagccaaac ggtaatcag gaagaacct gactggtta 
      gctggagat aacaacttg caagacatc caggaacag 
      gatactcga gatagagga tgcgggatt tcgacgaat 
      cggcagcag gctcagcgg aggattgtc ggaggcgag 
      gaagcagga tttgggact tttccttgg caggcttac 
      atccgaatc ggatcctcc agatgcgga ggttctcta 
      gtcagcagg cgccacgtc gttacagca ggtcattgt 
      gtagcacga gccaccccc agacaggtg catgtcacc 
      cttggagat tatgttatt aactcagca gtcgagccg 
      ctaccagct tacacattt ggggtttct cagatacag 
      gtgcatccc tttttcaag ttcaccccc caggccgac 
      cgcttcgac gtggccgtc cttcgcctg gaccgcacc 
      gctcaccag ctgccccac atcaccccc atctgcctt 
      cccccgcgc ggggagagc ttcctgggg gaggtgggc 
      gtcgcggcg ggctggggc gccctgagc cccggctcc 
      agactgaga ccgcagacg ctgcaagcc gtgcaagtg 
      ccagtgata gacaatcgc gtgtgcgag cggtggcac 
      aggtcgaag ggcatcggg gtgacgatc tacgacgag 
      atgatgtgc gcggggtac aagaacggc ggcagggac 
      agctgccaa ggcgactcc gggggcccc ctcatgctc 
      cagaagcag gggcggtgg ttcctcatc gggatcgtc 
      tcggcgggg tacagctgc gcccagccg ggccagccc 
      gggatctac caccgggtg gcgcatacc gttgactgg 
      atcacaagg gccatcggt gtttagtta cctctagag 
      tcgctacgg aaatgtaat ctgttcccg gaatcgggg 
      gagtgtcgg gatgagttc caaccaaac agtatttca 
      tgtaggtgg ttgaagcat tgcgacaaa gttcataaa 
      acggctgaa aggactttt aaaacctct ttctgttat 
      tagatttcc aaaagttgt gcttttaca aaaaacgtt 
      aaaaaagta ggacaaaaa ataatcgta gcgtctatg 
      ttataaata acgccattt ctcagtcat atagtgttg 
      taattcagt gttaattta ttttatact taataagtc 
      aactaccag aaccacaaa tctgacaag caatgatca 
      tttactcgt atgtacata cagaaatga aacttttat 
      tttgctccg gtggaaaag ttttaattt ctccttact 
      cttatagcc aataaaaat gtatatttt gtgatttgt 
      caatcaata catgagaaa gtaatttaa gtaccctgt 
      aattaactg taaattaaa ttttaaaac aggtttagt 
      aaattattt acataaatt attaaattc ttgtatatg 
      tacgattat aaaactgga tgttattta tgttatttt 
      gttgtagga ttattttaa gatgatgta cttgaacga 
      tatgactaa ataaaattt attttgaat t
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