| General information | |||
| TC008640 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 13517323..13531805 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC008640 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
For help on how to submit larger datasets or non experimental data please contact us.
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01377 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 50
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
- >TC008640 RA
MAEDDTKSI KSTKSTKST KSTKSKKDD KAVEDEEDE RLEFILAYF TKSYRLKQE KWNKMIAID ENKKLILNF LNNPQEKFM IITITSAGL LTPFKQYNS AIRSKYTYF IKKKEEVVT MENFADVIL FGDMAAKPI DELAVLLDT ILIPLLANP GNQKGWPQV VADDVVAHV RNFKNSVEQ IRGAMKSQT ILPMPQGMS RLFEAISQF EETEGTNID LVLKASLES GVTKWSNVC SEILKQSST IAFAGGNHP SPSAEVEFW KARLKNLEF IYDQLRDPR VKQVGKYLQ MTKSAYFST FKNMFKDIV AGVIEARDI CLYLKPMVT HFSNFEDGE LFENEHNIK PLLHCMGLI WANSKYYCA NEKMVILFR EIANMLIDA SNRMLDPSS IFQGEPDEM FEKIDKTLH ILELFMDSF EFVRDNLAT YFKREDEEM PALWTFHKR TVFQRLLDF IDRLRLIKA ILATNLEFS KLEKVEFGG IKGRLLSQK CEQIFDEFG TAYNVFNSI QYDPCDTSD LNILKDNND FHAKCADFD KRLAAIFSQ AFDDCYNLE SIFKFLAII GNLLERSVI RDEITPKYP RIIDIFNEE LDTVKIIFD EGKKNGIPI DKNFPPVAG TLMWISKLR QRIEKPGDD LKLLEDDII KSEEAHHSF EKYDHMMRI LDDEGKEVF GNWCKSIPQ QIETSMAKF ELIRKPENL IHLNFDPEL VAALREIRY MKFLEIPDV PQEALDLFE KVELLFECQ QKFNRVVNW YNHLKKNTL KVEYNLIEP EMKQIDELL QSVLDAITW STHDEATLS AIYEKVKDL SDRVVQCQK NMTAIEATL DAWVEAPLF ERREGKKEN LLYLEDRSE RTTKRFAQI ENSHEEIKK LLEVNYRLF FNIPEPVEE PVDESSPEE EVAEPPPTP KKGKKGKKP VETPKEKEK EKEKEKGKK EKGKKGGKG KKKKQKALE DAEAEMQEE PADPYENYR KEHWSKYLE YVDQLVKNK LVAAVHNSL VYLLNESGD LAPITPLFE LSLELHDPD IVFQPSINL TDDNNFVEE ITECVEDIF KMGEVMKRV NPESEDQHY FYEVKSDKN LAKMTEELL SRIMLMRED AFEYLKAFD EFTHIWTDD RHEYLRQFL LFGRLLSPE EHDRIAELK ETPPSVQQF KEQIDFYDD MYKKVEAMS NEKILEGDW MKIDVRPLK QAILNTICK WGNLFKQHL YDHVITSLN DLDSFIVEA IQAMQVTLE EDDYDGLLK VMGYLFKVK ERQLETDNM FQPLKEIMD LLFEYGMEF PEDVHVQLQ EIPDRWQQC KKVAATTKQ NVAPLQALQ VNAIKRRIN LFEIRQAMY RDMFKKLPF FNWSCKDVY SLLDKTNFE LTELETEMA KLQEQANLF ELTLPEFKP MNQARKEIR QIKQLWDYV HIFRSCIDE WKKTPWKKI DVEAMEMEC KKFGKEVRA MDKELRGWD VYIQLEAVI KNMLTSLRA VTELQNPAI RERHWKQLM QATKVKFVM DDSTTLSEL LALNLHEYE DEVKNIVDK SVKEMAMEK VLKELNATW TNMMFATEV HERTNLRLL VASEELIET LEENQVALQ NMMTSKFID FFLEEVSDW QKKLSNADQ VIQVFFEVQ RKWCYLESI FIGSEDIRS QLPEDSRRF EKIDRDFKE VLSEMLKDL NVVRSTNKP GLYDKLDGI LGQLILCEK ALNDYLETK RLAFPRFYF VSSADLLDI LSNGNQPDL IQRHLTKLF DSLAKLKFE KEGGKNSKR AYGMISKEN EEHVQFSQI CDCTGKVEV WLNRVIDVM RKTLQSCFA EAISTYDEK PREVWIYDY PAQPALCGT QIWWTTEVN IAFARLEEG YENALKDYQ RKQINQLNA LINLLLGDL TAGERQKIM TICTIDVHS RDVVAKMIA IKVENSQAF QWQSQLRHR WDDKIKDCF GNICDAQFR YQYEYLGNT PRLVITPLT DRCYITLTQ SLHLIMGGA PAGPAGTGK TETTKDLGK ALGMMVYVF NCSEQMDYK SIGNIYKGL SQTGCWGCF DEFNRISIE VLSVVAVQV KVIQDAIKD KKAKFNFLG QDIVLVPTV GLFITMNPG YAGRTELPE NLKALFRPC AMVVPDFAL ICEIMLVAE GFQEARLLA RKFITLYTL CRELLSKQD HYDWGLRAI KSVLVVAGS LKRGDRQRP EDQVLMRAL RDFNIPKIV TDDVPVFMG LIGDLFPAL DVPRKRDPD FEKTIKKSA VDLKLQPED GFVLKVVQL EELFAVRHS VFVIGNTGT GKTMVWKTL NKTYQNQKR KPIYNDINP KAVTNDELF GVINPATRE WKDGLFSVI MRDQANLTG DGPKWIVLD GDIDPMWIE SLNTLMDDN KVLTLASNE RIALTSAMR LLFEIASLR TATPATVSR AGILYINPA DLGWNPYVA SWIDTRTQQ NEKANLMIL FDKYVPPCL EILRHKLKK ITPISEVAH ISMLCFLLD CFLTPQNVP PDCPKEWYE IYFVFCTVW AFGSALFQD QIVDWRNEF SKWFVNEFK TIKFPVTGG NVFSYYVEE ETKRFAPWT ELVPEFELD PDIPLQATL VNTAETTRI RFFMDHLME KRIPVMLVG PAGSGKSVI VAEKLTSLS EHYAVTNVP FNFYTTSEM LQRILEKPL EKKAGRVFG PPGNKTMIY FVDDMNMPE VDKYFTVQP HTLIRQYMD YQHWYDRQK LTLKDIHNV QFVSCMNPT AGSFTINPR LQRHFCVFA VSFPSQEAS NHIYYSIIS QHFESEHNR FGKNIFKLS ASLVDAAVF LHQKVAASF LPTAVKFHY TFTLRDLSN IFQGMLFTT GDAIKTQTD LIRLWMHEC GRVYGDKLV EKRDLDTYK KLVIESVKK SFEELDENE VFEEPLIYC HFAEGMGDP KYFPIHEFD HLSKLLHEA LAGYNDLVA AMNLVLFED AMYHICRIN RIMEAPRGN ALLIGVGGS GKQSLTRLA SFISSFEVF QVQLKKGYS MTDLKADLA QLYLKAGLK SVGIVFLMT DAQVPQEIF LVAINDMLA SGEISELLP DEDVENVIN AVRGEVKQS GLQDTRENC WKFFIDRVR RLLKTVLCF SPVGSTLRV RARKFPALV NCTSIDWFH EWPREALES VSKRFLAEI EVLPKDLVP SVSNFMSDV HGVVNTMSE LYLQNEKRY NYTTPKSFL EQIALYSKL LYEKTKNLE DNIERLESG LLKLASTSE EVDGLKEVL AVQEVELNE KNEAAGKLI AVLSAENEK VAKEQAIAS EEEAKVKLI EEDVSVKQK ICADDLAKA EPALIAAQQ ALNTLNKNN LTELKSFTQ PPEAVVAVT AAVLILFSP KGRIPKDRS WKACKGLMT KVDKFLEDL INYDKENMQ PEVVKATLE YTKNPEFNP EKILSKSVA AAGLCAWVI NILKFYDVF VVVEPKRKA LQKANQELA DARAKLAEL NEKLNYLES QLAILKADF EEATAAKMK CQAEADATT QMIDLANRL VNGLASENI RWRELVKLY RKMIITLPG DVLMITCFI SYVGCFTRR YRTEMVEHH WIPSLKKIE PKIPMSEGL DPLSLLTDD AMVAGWNNE GLPNDRMST ENACILTNS ARWPLMIDP QLQGVKWIK TKYGDSLTV IRLSMKNYL DTIERCIQN GEIILVENI GETVDAVLD PVLGRVLVK KGKAIKIGD KEVDYNPNF RMIMHTKLA NPHYKPEIQ AQTTLINFT VTRDGLEEQ LLAEVVKAE RPDLESLKA NLTKQQNDF KIILKRCED NLLFRLSSA TGNILGDVE LVENLETTK KMSTEIEEK VKEAKITSV KIDEAREQY RPCSARASL LYFILNDLC RINAIYQFS LKAFSVVFK DALSKAVPA EKLKDRVVN LLDSITFSV FMYTTRGLF ECDKLIFMA QMAFQILLH AKEIDHAEL DFLLRYPVV PNLTTPVDF LTNQSWGGI KALSEFDDF RGLDKDLEG SAKRWRKFV DSECPEKEK FPGEWKNKT ALQHLCMMR ALRPDRMTY AVRCFIEEK LGAKYITAR SVEFAKSYE ETSSSTPVF FILSPGVNP LKDVEKLGK KLGFTFDSG NFHSISLGQ GQEIVAENA MDLASERGD WVILQNIHL VERWLSTLE KKMEQCAES AHQKFRLYL SAEPNADPQ VSVIPQGIL ESSIKITNE PPTGMYANL HKALDNFSQ ETLDMCTKE AEFKLILFA LCYFHAVVA ERRKFGPQG WNRNYPFNV GDLTISVNV LYNYLESNS KVPYEDLRY LFGEIMYGG HITDDWDRR LCRNYLMEY LQPELLEGD LQFAPGFFA PPNTDYVGY HRYIDDRLP PESPYLYGL HPNAEIGFL AYTSEVMFR TIFEMQPRD AGASVGHGF SREDKVKST LEDIQDRLP EEFPVAEMM AKVEDRTPY IIVAFQECD RMNSLTREM KRSLRELDL GLKGELTIT SDMEVLEEA LFMDQVPDS WSVKAYPSM LPLGQWVAD LGARIKELE GWVTDFSMP PTVWLGGFF NPQSFLTAI MQQTARKNE WPLDKMCLL TDVTKKQKE EFGAAPREG AYVHGLFME GARWDTQLG SIADAKLKE LFPVMPVIF IKAITQDKQ DLRNMYDCP VYKTRTRGP TFVWTFNLK TKEKASKWT LAGVAILLQ I
- >TC008640 RA
atggccgaa gatgatacc aagtctatc aaatcgacc aagtcgaca aagtcgacc aagtccacc aagtccaag aaagatgat aaggctgtc gaagacgag gaggatgaa cgactcgag ttcatcctc gcttatttt acaaaatcc tacagactc aagcaggaa aagtggaat aaaatgatc gcaatagac gaaaataag aaacttatt ttaaatttt ctgaacaac cctcaagag aaatttatg attattacg ataacatca gccggtctc ctgacccct tttaagcaa tacaacagc gctataaga tccaaatac acttatttc atcaaaaag aaggaggaa gttgtgact atggaaaac ttcgccgat gttattctt tttggcgac atggccgca aaacccatc gacgagttg gccgttctc ttggacaca attttgatc cctttgctt gcaaacccc gggaatcag aaaggatgg ccacaggtg gttgcagac gacgttgtc gcgcatgtc cggaatttt aaaaattca gtcgaacaa attcgggga gcaatgaaa agccaaaca atcttgcca atgccgcaa ggcatgtcc cggcttttc gaagcaata agccagttt gaagaaaca gaaggcact aatattgat ttggtcctg aaagcgtct ctcgaaagt ggtgtcacg aaatggtca aacgtttgc agcgagatt ctgaaacaa tcgtcaact attgctttc gccggcgga aatcatccg tcaccaagt gccgaagtg gaattctgg aaagctcgc ctcaagaat ctagaattt atttacgat caactgcga gatcccaga gtgaagcaa gttgggaaa tatttgcaa atgaccaaa agtgcctat ttctcaact tttaagaac atgttcaaa gacattgtt gctggcgtt atcgaagcg agagacatt tgtttgtat ttgaaacca atggtgact cacttctcg aatttcgaa gacggcgag ttgttcgaa aacgaacac aacatcaag cctttgctg cactgcatg ggcctgatt tgggccaac tcgaagtac tactgcgcc aacgagaaa atggtgatc ctgtttagg gaaatagca aatatgttg atcgacgcc agtaatcgt atgctagac cccagctca attttccaa ggcgaacct gacgaaatg ttcgaaaag attgacaag actctgcac atcttggag ctttttatg gactcgttc gagttcgtt cgcgacaat ttggccact tattttaag agagaggat gaagaaatg ccggcactt tggacgttt cataaaagg acggtgttt caaagactg ctcgacttt attgaccgt ctgaggctg attaaagca atcttggcg actaatcta gagttcagc aagttggag aaggttgag tttgggggt atcaaaggc cggttgttg tcccaaaag tgtgaacaa atttttgat gaattcggt accgcctac aatgttttt aattcgatt cagtatgat ccttgcgac accagtgat ttgaatatc ctcaaggat aacaacgat tttcacgca aaatgtgcc gactttgac aaaaggtta gcagcgatt ttctcccag gccttcgat gattgctac aacctcgaa agcatcttc aaatttctc gcaatcatc gggaatttg ctcgaaagg tccgttatc agagacgaa ataactcca aaataccca agaattatt gacatattc aacgaggaa cttgatacg gtgaaaatc atttttgac gaaggcaaa aagaatggt attcccatc gataaaaat ttccccccg gtcgctgga acccttatg tggatatct aaattgagg caaaggatt gaaaaacca ggcgatgat ttgaaatta ttagaagat gatattatc aagtccgag gaggctcat cattcgttt gaaaaatac gaccacatg atgcgaatt ttggacgat gagggcaag gaagtgttt ggaaattgg tgcaagtca attccgcaa caaatcgag actagtatg gccaagttc gaacttata cgtaaacct gagaacttg attcatttg aattttgat ccggaattg gtagcggcc ttgcgggaa attcggtac atgaagttc ttagaaatt ccggatgtg cctcaagag gcgttagat ctgttcgaa aaggttgaa cttttgttc gaatgtcaa cagaaattt aacagagtt gtgaattgg tataaccat ctcaagaag aacacgctt aaggtggaa tacaactta attgagccg gaaatgaaa caaatagat gagttgctc caatcagtg cttgatgct attacgtgg agcactcat gatgaggca actcttagt gccatttat gaaaaagtt aaagatctg tcggatcgt gttgtgcaa tgtcagaag aatatgact gctatcgaa gccacgctg gatgcctgg gttgaggct ccactgttt gaaaggaga gaaggcaaa aaagagaat ctgctatat ctcgaagac cggtctgaa cgtactact aaaagattt gctcaaatt gagaatagc cacgaagaa ataaaaaaa ctcctagaa gttaactat cgattgttc tttaatatt cctgagccg gttgaagaa ccggttgat gagtcgtcg cctgaagaa gaagttgct gagccacca cccacccca aaaaaaggt aaaaaaggg aaaaagcca gtcgaaaca cccaaagaa aaggagaaa gagaaagaa aaagaaaaa gggaagaag gagaaaggt aagaaaggt ggaaaaggc aaaaagaag aagcaaaaa gcccttgaa gacgctgaa gctgagatg caggaagaa cctgcagat ccttacgag aattatcgt aaagaacac tggagcaaa tatttggaa tatgttgat caactagtc aaaaacaaa cttgtagct gcagtccat aatagtttg gtttacttg ctgaatgag tctggcgat ttagctcct attacacct ttgtttgaa ctgtcttta gaacttcat gatcctgac attgtgttt caaccttcg attaatcta acagatgat aataatttc gttgaagaa ataaccgaa tgcgtggaa gatattttc aaaatgggt gaagttatg aaacgggtg aatccggaa agtgaagac cagcattac ttttacgaa gtcaaatca gataaaaat ttggccaaa atgactgaa gagcttctg agcagaatt atgttgatg agagaagat gcgtttgaa tatcttaaa gcatttgac gaatttacg catatttgg accgacgat aggcacgag tatttgcgc cagttcctg ctttttggc cggttgttg agtcccgag gagcacgac agaatcgcg gaactaaaa gagacccca ccgtctgtg caacagttt aaggagcaa attgatttt tacgacgat atgtacaaa aaggtcgaa gctatgagc aatgaaaag attcttgaa ggtgactgg atgaaaatt gatgtccgg cccttgaaa caagctatc cttaacacg atttgcaaa tggggcaat cttttcaag cagcatctt tacgatcac gttattact agcctcaat gatctcgac agtttcatt gtggaggca attcaagcc atgcaagtc acattagag gaggacgac tacgacggc ttattaaaa gtgatggga tatttgttt aaagtgaag gaaagacag ttggaaacc gataatatg ttccagcca ttgaaggaa atcatggat ctgctgttc gaatatggg atggaattt ccggaagac gtgcacgtc caattacag gagattcca gacaggtgg caacaatgc aagaaagtt gccgcaact actaaacaa aacgttgca ccacttcaa gcccttcaa gtgaatgcg atcaaaaga cgaattaat ttgttcgaa atccgacaa gcgatgtat agagacatg tttaagaaa cttcccttc tttaactgg agctgtaaa gatgtgtac tctttgtta gataaaact aatttcgaa ctgactgaa cttgagaca gaaatggcg aaattgcaa gagcaagcc aatttattc gaactaacg ctgcctgag tttaaacca atgaatcag gcgaggaag gaaattagg caaattaaa cagttgtgg gactacgtc catattttc cggtcgtgt atcgacgaa tggaagaaa accccctgg aaaaaaatt gacgttgag gcgatggaa atggaatgt aaaaaattc ggcaaggag gtgcgagcc atggataag gaattaagg ggttgggat gtttacatc cagctggaa gcggtaata aagaatatg ttaacgtcg ttacgagcc gtcaccgaa ttgcaaaat ccggcgatt agggagagg cactggaag cagttgatg caagctacg aaggtgaaa tttgtaatg gacgattcg accacactc agcgaattg ctcgccttg aatttgcac gagtatgaa gatgaagtt aagaacatt gttgataaa tcagtcaaa gaaatggcg atggagaaa gtcctaaaa gaattaaac gcaacctgg actaacatg atgtttgcg accgaagtc cacgaacgc acaaatttg agacttttg gtggcttcc gaagagtta attgagact cttgaagag aaccaagtt gctctccaa aacatgatg acgtccaaa tttatcgat ttcttcctt gaagaggtt tccgactgg cagaaaaaa ttatcgaat gctgatcaa gtcatacaa gtgtttttc gaagtgcag cgcaagtgg tgctacttg gaaagtatt ttcataggg tctgaagac atcagaagc caattacct gaagattca agacggttt gagaaaatc gatagggat tttaaagag gttttgtcc gaaatgctg aaggattta aatgtggtg aggtccacc aacaagccc ggactttac gacaagctt gacggtatt ttggggcaa ttaattttg tgcgaaaaa gccctcaac gactatttg gaaacgaaa agattggca tttcctcgc ttctacttc gtatcgtct gccgattta ttagatatt ttgtctaac ggaaatcaa ccagatctc attcagaga catttgacc aaactattt gattctctg gctaaactg aaatttgaa aaagaaggt gggaaaaat tcaaagaga gcctatgga atgatttcc aaagaaaat gaagaacat gtgcaattc agccaaatt tgtgactgc actggcaaa gtggaagtt tggttaaat cgtgttatt gatgtaatg agaaaaacc cttcaaagt tgtttcgct gaggctata tcaacctac gatgaaaaa cctagagag gtttggatt tacgactat ccagcacaa ccggcactt tgcggcacg caaatttgg tggacaact gaggtaaac attgctttc gcaagactg gaagaaggt tacgaaaac gccctaaaa gactatcaa agaaaacaa atcaatcag ttaaatgcc cttattaat ctcttgttg ggtgatttg actgcaggc gagcgccaa aagatcatg accatttgt accatcgac gtgcactcg agagacgta gtcgcgaaa atgattgca atcaaagtg gagaattca caagccttt cagtggcaa agtcagttg cgacatcgc tgggacgat aaaatcaaa gactgtttt ggtaatatc tgtgacgcc cagttcaga taccaatac gaatatttg ggcaacacc ccccgactg gtgataaca cccttaact gatcgttgc tacataact ctgactcaa agcttgcat ttaattatg gggggagca cctgccggg cctgcagga accgggaaa actgagaca accaaagat ttggggaaa gctttaggg atgatggtt tacgtgttc aactgttcg gaacaaatg gactataag tctattggg aacatttac aaagggtta tcacagacg ggttgttgg ggttgtttt gacgaattt aaccgaatt tccattgag gtactgtct gtggtagcc gtccaggtg aaagtcatc caggacgct atcaaggac aagaaagct aagtttaat ttcttgggg caagatatt gtcttagtc cccacggtc ggccttttc attaccatg aacccgggc tacgccggc cgtactgaa ctcccggaa aatctaaaa gccctattc aggccatgc gctatggtt gtgcctgac ttcgcccta atttgcgaa atcatgttg gtcgccgaa ggtttccaa gaggctcgt cttctggcg cgcaaattc atcactctc tacacgttg tgtcgcgaa ttattgagc aaacaagac cattacgat tggggtttg agggctatt aagtctgtt ttggtcgtt gcagggtcg ttgaaacgt ggcgataga caacggccg gaagaccaa gtcttaatg cgcgctttg cgtgatttc aacatccct aaaatcgtt acagacgat gtcccggtt tttatggga ctgataggt gatttgttt ccggccttg gacgtgccg agaaagcgt gacccggac tttgaaaaa acgataaaa aaatctgcg gttgatttg aaattgcaa cctgaggac ggtttcgtc ttgaaagtg gtgcaattg gaggagctt ttcgctgtg cgacattca gtattcgtg attggtaac acgggaact ggtaaaact atggtttgg aagactttg aacaagact tatcagaac cagaaaaga aagccgatt tataacgat attaatccc aaagctgtg actaatgat gaattgttt ggggtgata aacccggcc actagagag tggaaagat ggtttgttt tctgtaatc atgagagat caggctaat ttaaccggc gatgggcct aaatggatt gttcttgat ggtgacatt gatcccatg tggattgaa tcattaaac acacttatg gatgataat aaagtgcta actttggcc agcaatgaa cgaattgct ttaacttca gctatgcga ttattattt gaaattgcc tctttacgt actgcaacc ccagccact gtttcaaga gccggtatt ttatacata aacccggcc gatctgggt tggaaccct tacgtagct tcctggatt gataccaga acccaacaa aatgaaaaa gccaatctt atgatactc tttgacaag tatgttcct ccttgtttg gaaatactt cgtcacaaa ttgaaaaaa ataacacca atctctgaa gtagcgcat atttcgatg ctttgcttc ctcttggac tgtttccta acaccccag aatgtccct cctgattgt cccaaagag tggtacgag atttacttc gtattttgc acagtctgg gcgtttggt tcggctttg ttccaagac caaattgtc gactggcgc aatgaattt agcaaatgg tttgtgaat gaattcaaa actatcaaa tttcccgtc actggaggc aacgtcttc agttattac gttgaagag gaaactaaa aggtttgct ccatggacg gaactagta cccgaattt gagcttgat cccgatatt cccttgcaa gccactttg gtcaatacg gccgaaact accaggatt cggtttttc atggatcat ttaatggaa aaacgaatt cctgtcatg ttggtaggg ccggcagga agtggcaaa agtgtgatt gttgcggaa aaactcacc agcttatcc gaacattac gccgttaca aatgtccct ttcaatttt tacactacc tctgaaatg ttgcaaaga attttggaa aaaccctta gaaaagaaa gctgggcgt gtgtttggc cctccgggt aataagact atgatttat ttcgtggac gatatgaac atgcccgaa gttgacaag tattttact gtacaacct cacacgtta attcgccag tatatggac taccagcat tggtatgac cgtcagaaa ctgacttta aaggacatt cacaatgtt cagtttgtc tcctgcatg aacccaacc gccggctct ttcaccata aacccacgg ttgcagaga cacttttgc gtctttgcc gtgagtttt ccgagccaa gaggcgtcg aaccatatc tattacagt attatatcg caacatttt gagtcggaa cataacagg tttggcaaa aatattttc aaactgagt gcttctcta gtggatgcg gcggttttt ctgcaccag aaagtagcg gctagtttt ctgccaacc gctgtcaag tttcattac acatttact ttgcgagac ctttcgaat atttttcaa ggaatgctt ttcacgacc ggagacgct attaaaact cagaccgat ttgatccgg ctttggatg cacgaatgt ggccgcgtt tacggtgac aaactggtc gaaaaacgc gatttggac acttacaaa aagctggta attgagtca gtgaagaaa agtttcgag gagttggac gaaaatgag gtgtttgag gaacctttg atttattgt cattttgct gagggcatg ggggatccg aaatacttc cccattcat gaatttgac catttgagt aaactcttg cacgaagct ttggccggt tataatgat ctagtggcg gcgatgaat ttggttttg tttgaagat gcaatgtat cacatttgc cgcattaac agaattatg gaagctcca agaggtaac gcgctgctc attggagtt ggaggttca ggaaagcag tctttgacg agacttgct tccttcatc tcctcgttt gaagtgttt caagtccag ttgaagaaa ggttactct atgactgac ctaaaggcc gatttggcc caattatat ctaaaagcg ggtttgaag agcgttgga attgtgttt ctgatgact gatgcccaa gtccctcaa gagattttc ctggttgcg attaacgat atgttggca tcgggcgaa atcagtgag ttactgccc gacgaagac gttgaaaat gttatcaat gcagttaga ggtgaggtg aagcaaagt gggttacaa gacacacgg gagaattgt tggaaattt ttcattgat cgtgtccgg agattatta aaaacggtg ttgtgtttc tcaccagtt ggttcgact ctacgagtg cgagcgaga aaatttccg gctcttgtt aactgtact tctatcgac tggttccat gaatggccg agggaagcc ctggaatca gtatcaaag agatttttg gcggaaatt gaagttctc cccaaagat ttagttcct tcagtttcc aacttcatg tcagatgtt cacggagtg gtgaacacc atgtccgag ttgtatcta caaaatgag aaacgctac aactacacc actcctaaa tctttccta gaacagatc gccttgtat tctaaactt ctttacgaa aagactaaa aatttggag gacaatatc gaacgcctc gagtctggt ttactcaaa ctggcatca acttcggaa gaagtggac ggtttgaaa gaagtgttg gcagtccaa gaggtagaa ttgaatgaa aagaatgaa gccgccggt aaactcatt gccgtccta agtgccgaa aacgaaaaa gttgcaaaa gaacaggca atcgctagc gaggaagaa gccaaagtt aaactaatc gaggaggat gttagtgtc aaacaaaaa atctgtgcc gatgatttg gccaaagcc gagccagcg ctaatcgca gcccaacaa gctttaaac accctgaat aaaaacaac ttaactgaa ctgaaatcc ttcacgcaa ccgcctgaa gcggtagtc gctgtaaca gccgctgtt ttaatcctg ttttcgcca aaaggacga attccgaaa gatcgtagc tggaaagct tgtaagggg ttgatgaca aaagtcgat aaattttta gaagattta ataaactat gataaggaa aacatgcaa ccggaagta gtgaaagca actctggaa tatacgaaa aatcccgag ttcaatcca gaaaaaatt ttgagcaag tcggtggca gctgctgga ttgtgtgcg tgggtcatt aatattttg aaattttac gacgtgttt gtcgtggtc gaacccaag aggaaagct ttgcaaaaa gccaatcag gaactggca gatgcgaga gctaaactg gccgagctt aacgagaaa ttgaattat ctcgagtca caattggcg attttgaag gccgatttt gaggaagct acagccgcg aagatgaaa tgtcaggcc gaagctgat gctacgact caaatgatc gatttagct aatcgactt gttaatggt ttggcgtcg gaaaatatc aggtggcga gagttggtc aagttgtac cggaaaatg ataattaca ctgccaggg gatgtgttg atgataact tgctttatt tcgtacgtt ggatgcttc acgagacgg tacagaacc gagatggtt gaacatcac tggataccc tcgctgaaa aaaatcgag ccgaaaatt ccgatgagt gaagggctg gatccctta tccctgctc acagacgat gcaatggtc gcaggatgg aataatgag gggcttcct aatgatcgt atgtcaacc gagaatgct tgtatttta acaaattcg gcaagatgg cccctgatg attgatccc caattgcaa ggagttaag tggatcaaa actaaatac ggcgatagc ttgacagtt attcgactg agcatgaaa aattacttg gacactatt gaaagatgt atccaaaat ggtgaaatt atccttgtt gaaaacata ggcgagacc gtggacgcg gtcttagac cctgttctg ggtcgagtc ttagtcaaa aaaggcaag gcgataaaa atcggtgat aaggaagtg gattacaac cccaacttt aggatgatc atgcacacg aaactggca aatccgcat tacaaaccg gaaattcaa gcacaaact accctcatc aacttcaca gtaacgcga gatggttta gaggagcag cttttggca gaagtcgtc aaagctgaa cgcccagac ttggaaagt ttgaaagcg aatttaacg aaacaacaa aacgatttc aaaattatt ttgaaaaga tgtgaggat aatttattg tttagactt tcctcggcc acgggtaac atactgggc gatgtggaa ctagtcgag aatttggaa acaaccaag aaaatgtcc acggaaatt gaagagaaa gtcaaagaa gctaaaata acttcagtc aaaatcgac gaagcgcgt gaacagtac cgaccatgt tcggctaga gcttcgctc ctttatttc attttgaac gatctttgc cgcatcaac gcaatctat caattctca ttgaaggcc ttcagtgtg gtgtttaag gacgcgtta tctaaagca gtaccggcc gaaaagtta aaagatcgg gtagttaac ttattggac agtataact ttttcggta tttatgtac acaactcga ggtctgttc gaatgtgac aagttgatt ttcatggcc caaatggca tttcaaatt ttgctgcat gctaaagaa atcgatcac gctgaactt gactttctg ttacggtac cctgtagta ccgaacttg acaacccct gtggacttc ctcacaaat cagtcctgg ggcggcatt aaagccctg tcagagttt gacgatttc agaggttta gacaaagac ttggaaggc tcggcgaaa cgttggcga aaattcgtc gatagtgaa tgccccgaa aaagaaaag ttccccggc gagtggaaa aacaaaacc gctttgcaa cacttgtgc atgatgcgt gccctccgc cccgaccgt atgacctac gctgttcgt tgtttcatt gaagagaaa ctcggagcc aaatacata acagccaga tccgtcgaa tttgcaaaa tcttacgaa gaaacaagc tcttcaacc cccgtgttc tttattcta tcgccaggc gtaaatccg ctcaaggat gtagaaaag ctgggcaaa aagctgggt ttcaccttt gattctggt aacttccac agtatttcc ttgggccaa ggccaggaa atcgtggct gaaaacgca atggatttg gcgtcggag cggggcgat tgggtcatt ttgcaaaac attcattta gtcgagcgg tggttgtcc actttggag aaaaaaatg gaacaatgc gccgaaagc gcccatcaa aagtttagg ttgtatttg agcgctgag cctaacgct gatccccaa gtgtccgta atcccgcaa gggattcta gaatcgtcg atcaaaatc acaaacgag ccgccgact ggcatgtat gccaattta cacaaagct ttggacaat ttcagccag gaaacgtta gacatgtgc acgaaagaa gcggagttt aagctgatt ttgttcgct ttgtgctat ttccatgcc gtggttgcc gaaagacgg aaatttggg ccacaaggg tggaacagg aattacccc tttaatgtt ggcgatctc acaattagc gtaaacgtg ttgtacaat tatctggaa agtaacagt aaggtcccc tacgaagat ttgcgctat ctgtttggg gaaataatg tacgggggg catattacg gacgattgg gatcgacgg ttgtgccga aattatcta atggagtat ctccagccc gaattgctg gaaggagac ttgcagttt gcgccggga ttttttgcc ccacctaat acagactat gttgggtat catcgctat attgacgac cgtttgccg cctgaaagc ccctacctt tacggccta cacccaaac gcggaaatt ggcttcttg gcctacact tctgaagtt atgtttaga acaattttc gaaatgcaa ccgcgagat gcgggagct tcggtaggc catggattt tctcgagag gataaggtt aaatcaacc cttgaagat atccaagac cggctcccc gaggagttt ccggtcgcc gaaatgatg gcaaaagtc gaagacagg accccgtac ataattgtg gcgttccag gagtgtgac agaatgaac agtttaaca cgggagatg aagagatcg cttcgggag ctcgacttg ggattaaag ggtgaactt acgattacg tcggacatg gaggtgttg gaagaggcc ctatttatg gaccaagtc cctgactcg tggtccgta aaggcctac cccagcatg ctgcctttg ggtcagtgg gtggccgac ttgggggct agaattaag gagctggag gggtgggtt accgatttc tcgatgcct cccaccgtc tggctgggg ggtttcttc aacccccag tcgttcctc actgccatt atgcaacaa acggctcgc aaaaacgaa tggcctttg gacaaaatg tgtctgttg accgacgtc accaaaaag cagaaagag gagttcggg gcggctcct cgggaaggc gcatatgtg cacggttta tttatggag ggcgcaagg tgggatacg cagctcgga agtattgcc gatgctaag cttaaagaa ttgttccct gtaatgccg gttatattc atcaaggca atcacgcag gataagcag gatttacgg aatatgtac gactgtcct gtttacaaa acaaggact cggggaccg actttcgtc tggactttt aatttaaaa acgaaggag aaggcgtcc aagtggacc cttgcagga gtagcgatt ctgttacag atttaa
- >TC008640 RA
agctgttta ttattagca aacagaccg ttaaggtct gacaagtgt tcaaacttt ttaaaaaac tcctgtaat taaatggcc gaagatgat accaagtct atcaaatcg accaagtcg acaaagtcg accaagtcc accaagtcc aagaaagat gataaggct gtcgaagac gaggaggat gaacgactc gagttcatc ctcgcttat tttacaaaa tcctacaga ctcaagcag gaaaagtgg aataaaatg atcgcaata gacgaaaat aagaaactt attttaaat tttctgaac aaccctcaa gagaaattt atgattatt acgataaca tcagccggt ctcctgacc ccttttaag caatacaac agcgctata agatccaaa tacacttat ttcatcaaa aagaaggag gaagttgtg actatggaa aacttcgcc gatgttatt ctttttggc gacatggcc gcaaaaccc atcgacgag ttggccgtt ctcttggac acaattttg atccctttg cttgcaaac cccgggaat cagaaagga tggccacag gtggttgca gacgacgtt gtcgcgcat gtccggaat tttaaaaat tcagtcgaa caaattcgg ggagcaatg aaaagccaa acaatcttg ccaatgccg caaggcatg tcccggctt ttcgaagca ataagccag tttgaagaa acagaaggc actaatatt gatttggtc ctgaaagcg tctctcgaa agtggtgtc acgaaatgg tcaaacgtt tgcagcgag attctgaaa caatcgtca actattgct ttcgccggc ggaaatcat ccgtcacca agtgccgaa gtggaattc tggaaagct cgcctcaag aatctagaa tttatttac gatcaactg cgagatccc agagtgaag caagttggg aaatatttg caaatgacc aaaagtgcc tatttctca acttttaag aacatgttc aaagacatt gttgctggc gttatcgaa gcgagagac atttgtttg tatttgaaa ccaatggtg actcacttc tcgaatttc gaagacggc gagttgttc gaaaacgaa cacaacatc aagcctttg ctgcactgc atgggcctg atttgggcc aactcgaag tactactgc gccaacgag aaaatggtg atcctgttt agggaaata gcaaatatg ttgatcgac gccagtaat cgtatgcta gaccccagc tcaattttc caaggcgaa cctgacgaa atgttcgaa aagattgac aagactctg cacatcttg gagcttttt atggactcg ttcgagttc gttcgcgac aatttggcc acttatttt aagagagag gatgaagaa atgccggca ctttggacg tttcataaa aggacggtg tttcaaaga ctgctcgac tttattgac cgtctgagg ctgattaaa gcaatcttg gcgactaat ctagagttc agcaagttg gagaaggtt gagtttggg ggtatcaaa ggccggttg ttgtcccaa aagtgtgaa caaattttt gatgaattc ggtaccgcc tacaatgtt tttaattcg attcagtat gatccttgc gacaccagt gatttgaat atcctcaag gataacaac gattttcac gcaaaatgt gccgacttt gacaaaagg ttagcagcg attttctcc caggccttc gatgattgc tacaacctc gaaagcatc ttcaaattt ctcgcaatc atcgggaat ttgctcgaa aggtccgtt atcagagac gaaataact ccaaaatac ccaagaatt attgacata ttcaacgag gaacttgat acggtgaaa atcattttt gacgaaggc aaaaagaat ggtattccc atcgataaa aatttcccc ccggtcgct ggaaccctt atgtggata tctaaattg aggcaaagg attgaaaaa ccaggcgat gatttgaaa ttattagaa gatgatatt atcaagtcc gaggaggct catcattcg tttgaaaaa tacgaccac atgatgcga attttggac gatgagggc aaggaagtg tttggaaat tggtgcaag tcaattccg caacaaatc gagactagt atggccaag ttcgaactt atacgtaaa cctgagaac ttgattcat ttgaatttt gatccggaa ttggtagcg gccttgcgg gaaattcgg tacatgaag ttcttagaa attccggat gtgcctcaa gaggcgtta gatctgttc gaaaaggtt gaacttttg ttcgaatgt caacagaaa tttaacaga gttgtgaat tggtataac catctcaag aagaacacg cttaaggtg gaatacaac ttaattgag ccggaaatg aaacaaata gatgagttg ctccaatca gtgcttgat gctattacg tggagcact catgatgag gcaactctt agtgccatt tatgaaaaa gttaaagat ctgtcggat cgtgttgtg caatgtcag aagaatatg actgctatc gaagccacg ctggatgcc tgggttgag gctccactg tttgaaagg agagaaggc aaaaaagag aatctgcta tatctcgaa gaccggtct gaacgtact actaaaaga tttgctcaa attgagaat agccacgaa gaaataaaa aaactccta gaagttaac tatcgattg ttctttaat attcctgag ccggttgaa gaaccggtt gatgagtcg tcgcctgaa gaagaagtt gctgagcca ccacccacc ccaaaaaaa ggtaaaaaa gggaaaaag ccagtcgaa acacccaaa gaaaaggag aaagagaaa gaaaaagaa aaagggaag aaggagaaa ggtaagaaa ggtggaaaa ggcaaaaag aagaagcaa aaagccctt gaagacgct gaagctgag atgcaggaa gaacctgca gatccttac gagaattat cgtaaagaa cactggagc aaatatttg gaatatgtt gatcaacta gtcaaaaac aaacttgta gctgcagtc cataatagt ttggtttac ttgctgaat gagtctggc gatttagct cctattaca cctttgttt gaactgtct ttagaactt catgatcct gacattgtg tttcaacct tcgattaat ctaacagat gataataat ttcgttgaa gaaataacc gaatgcgtg gaagatatt ttcaaaatg ggtgaagtt atgaaacgg gtgaatccg gaaagtgaa gaccagcat tacttttac gaagtcaaa tcagataaa aatttggcc aaaatgact gaagagctt ctgagcaga attatgttg atgagagaa gatgcgttt gaatatctt aaagcattt gacgaattt acgcatatt tggaccgac gataggcac gagtatttg cgccagttc ctgcttttt ggccggttg ttgagtccc gaggagcac gacagaatc gcggaacta aaagagacc ccaccgtct gtgcaacag tttaaggag caaattgat ttttacgac gatatgtac aaaaaggtc gaagctatg agcaatgaa aagattctt gaaggtgac tggatgaaa attgatgtc cggcccttg aaacaagct atccttaac acgatttgc aaatggggc aatcttttc aagcagcat ctttacgat cacgttatt actagcctc aatgatctc gacagtttc attgtggag gcaattcaa gccatgcaa gtcacatta gaggaggac gactacgac ggcttatta aaagtgatg ggatatttg tttaaagtg aaggaaaga cagttggaa accgataat atgttccag ccattgaag gaaatcatg gatctgctg ttcgaatat gggatggaa tttccggaa gacgtgcac gtccaatta caggagatt ccagacagg tggcaacaa tgcaagaaa gttgccgca actactaaa caaaacgtt gcaccactt caagccctt caagtgaat gcgatcaaa agacgaatt aatttgttc gaaatccga caagcgatg tatagagac atgtttaag aaacttccc ttctttaac tggagctgt aaagatgtg tactctttg ttagataaa actaatttc gaactgact gaacttgag acagaaatg gcgaaattg caagagcaa gccaattta ttcgaacta acgctgcct gagtttaaa ccaatgaat caggcgagg aaggaaatt aggcaaatt aaacagttg tgggactac gtccatatt ttccggtcg tgtatcgac gaatggaag aaaaccccc tggaaaaaa attgacgtt gaggcgatg gaaatggaa tgtaaaaaa ttcggcaag gaggtgcga gccatggat aaggaatta aggggttgg gatgtttac atccagctg gaagcggta ataaagaat atgttaacg tcgttacga gccgtcacc gaattgcaa aatccggcg attagggag aggcactgg aagcagttg atgcaagct acgaaggtg aaatttgta atggacgat tcgaccaca ctcagcgaa ttgctcgcc ttgaatttg cacgagtat gaagatgaa gttaagaac attgttgat aaatcagtc aaagaaatg gcgatggag aaagtccta aaagaatta aacgcaacc tggactaac atgatgttt gcgaccgaa gtccacgaa cgcacaaat ttgagactt ttggtggct tccgaagag ttaattgag actcttgaa gagaaccaa gttgctctc caaaacatg atgacgtcc aaatttatc gatttcttc cttgaagag gtttccgac tggcagaaa aaattatcg aatgctgat caagtcata caagtgttt ttcgaagtg cagcgcaag tggtgctac ttggaaagt attttcata gggtctgaa gacatcaga agccaatta cctgaagat tcaagacgg tttgagaaa atcgatagg gattttaaa gaggttttg tccgaaatg ctgaaggat ttaaatgtg gtgaggtcc accaacaag cccggactt tacgacaag cttgacggt attttgggg caattaatt ttgtgcgaa aaagccctc aacgactat ttggaaacg aaaagattg gcatttcct cgcttctac ttcgtatcg tctgccgat ttattagat attttgtct aacggaaat caaccagat ctcattcag agacatttg accaaacta tttgattct ctggctaaa ctgaaattt gaaaaagaa ggtgggaaa aattcaaag agagcctat ggaatgatt tccaaagaa aatgaagaa catgtgcaa ttcagccaa atttgtgac tgcactggc aaagtggaa gtttggtta aatcgtgtt attgatgta atgagaaaa acccttcaa agttgtttc gctgaggct atatcaacc tacgatgaa aaacctaga gaggtttgg atttacgac tatccagca caaccggca ctttgcggc acgcaaatt tggtggaca actgaggta aacattgct ttcgcaaga ctggaagaa ggttacgaa aacgcccta aaagactat caaagaaaa caaatcaat cagttaaat gcccttatt aatctcttg ttgggtgat ttgactgca ggcgagcgc caaaagatc atgaccatt tgtaccatc gacgtgcac tcgagagac gtagtcgcg aaaatgatt gcaatcaaa gtggagaat tcacaagcc tttcagtgg caaagtcag ttgcgacat cgctgggac gataaaatc aaagactgt tttggtaat atctgtgac gcccagttc agataccaa tacgaatat ttgggcaac accccccga ctggtgata acaccctta actgatcgt tgctacata actctgact caaagcttg catttaatt atgggggga gcacctgcc gggcctgca ggaaccggg aaaactgag acaaccaaa gatttgggg aaagcttta gggatgatg gtttacgtg ttcaactgt tcggaacaa atggactat aagtctatt gggaacatt tacaaaggg ttatcacag acgggttgt tggggttgt tttgacgaa tttaaccga atttccatt gaggtactg tctgtggta gccgtccag gtgaaagtc atccaggac gctatcaag gacaagaaa gctaagttt aatttcttg gggcaagat attgtctta gtccccacg gtcggcctt ttcattacc atgaacccg ggctacgcc ggccgtact gaactcccg gaaaatcta aaagcccta ttcaggcca tgcgctatg gttgtgcct gacttcgcc ctaatttgc gaaatcatg ttggtcgcc gaaggtttc caagaggct cgtcttctg gcgcgcaaa ttcatcact ctctacacg ttgtgtcgc gaattattg agcaaacaa gaccattac gattggggt ttgagggct attaagtct gttttggtc gttgcaggg tcgttgaaa cgtggcgat agacaacgg ccggaagac caagtctta atgcgcgct ttgcgtgat ttcaacatc cctaaaatc gttacagac gatgtcccg gtttttatg ggactgata ggtgatttg tttccggcc ttggacgtg ccgagaaag cgtgacccg gactttgaa aaaacgata aaaaaatct gcggttgat ttgaaattg caacctgag gacggtttc gtcttgaaa gtggtgcaa ttggaggag cttttcgct gtgcgacat tcagtattc gtgattggt aacacggga actggtaaa actatggtt tggaagact ttgaacaag acttatcag aaccagaaa agaaagccg atttataac gatattaat cccaaagct gtgactaat gatgaattg tttggggtg ataaacccg gccactaga gagtggaaa gatggtttg ttttctgta atcatgaga gatcaggct aatttaacc ggcgatggg cctaaatgg attgttctt gatggtgac attgatccc atgtggatt gaatcatta aacacactt atggatgat aataaagtg ctaactttg gccagcaat gaacgaatt gctttaact tcagctatg cgattatta tttgaaatt gcctcttta cgtactgca accccagcc actgtttca agagccggt attttatac ataaacccg gccgatctg ggttggaac ccttacgta gcttcctgg attgatacc agaacccaa caaaatgaa aaagccaat cttatgata ctctttgac aagtatgtt cctccttgt ttggaaata cttcgtcac aaattgaaa aaaataaca ccaatctct gaagtagcg catatttcg atgctttgc ttcctcttg gactgtttc ctaacaccc cagaatgtc cctcctgat tgtcccaaa gagtggtac gagatttac ttcgtattt tgcacagtc tgggcgttt ggttcggct ttgttccaa gaccaaatt gtcgactgg cgcaatgaa tttagcaaa tggtttgtg aatgaattc aaaactatc aaatttccc gtcactgga ggcaacgtc ttcagttat tacgttgaa gaggaaact aaaaggttt gctccatgg acggaacta gtacccgaa tttgagctt gatcccgat attcccttg caagccact ttggtcaat acggccgaa actaccagg attcggttt ttcatggat catttaatg gaaaaacga attcctgtc atgttggta gggccggca ggaagtggc aaaagtgtg attgttgcg gaaaaactc accagctta tccgaacat tacgccgtt acaaatgtc cctttcaat ttttacact acctctgaa atgttgcaa agaattttg gaaaaaccc ttagaaaag aaagctggg cgtgtgttt ggccctccg ggtaataag actatgatt tatttcgtg gacgatatg aacatgccc gaagttgac aagtatttt actgtacaa cctcacacg ttaattcgc cagtatatg gactaccag cattggtat gaccgtcag aaactgact ttaaaggac attcacaat gttcagttt gtctcctgc atgaaccca accgccggc tctttcacc ataaaccca cggttgcag agacacttt tgcgtcttt gccgtgagt tttccgagc caagaggcg tcgaaccat atctattac agtattata tcgcaacat tttgagtcg gaacataac aggtttggc aaaaatatt ttcaaactg agtgcttct ctagtggat gcggcggtt tttctgcac cagaaagta gcggctagt tttctgcca accgctgtc aagtttcat tacacattt actttgcga gacctttcg aatattttt caaggaatg cttttcacg accggagac gctattaaa actcagacc gatttgatc cggctttgg atgcacgaa tgtggccgc gtttacggt gacaaactg gtcgaaaaa cgcgatttg gacacttac aaaaagctg gtaattgag tcagtgaag aaaagtttc gaggagttg gacgaaaat gaggtgttt gaggaacct ttgatttat tgtcatttt gctgagggc atgggggat ccgaaatac ttccccatt catgaattt gaccatttg agtaaactc ttgcacgaa gctttggcc ggttataat gatctagtg gcggcgatg aatttggtt ttgtttgaa gatgcaatg tatcacatt tgccgcatt aacagaatt atggaagct ccaagaggt aacgcgctg ctcattgga gttggaggt tcaggaaag cagtctttg acgagactt gcttccttc atctcctcg tttgaagtg tttcaagtc cagttgaag aaaggttac tctatgact gacctaaag gccgatttg gcccaatta tatctaaaa gcgggtttg aagagcgtt ggaattgtg tttctgatg actgatgcc caagtccct caagagatt ttcctggtt gcgattaac gatatgttg gcatcgggc gaaatcagt gagttactg cccgacgaa gacgttgaa aatgttatc aatgcagtt agaggtgag gtgaagcaa agtgggtta caagacaca cgggagaat tgttggaaa tttttcatt gatcgtgtc cggagatta ttaaaaacg gtgttgtgt ttctcacca gttggttcg actctacga gtgcgagcg agaaaattt ccggctctt gttaactgt acttctatc gactggttc catgaatgg ccgagggaa gccctggaa tcagtatca aagagattt ttggcggaa attgaagtt ctccccaaa gatttagtt ccttcagtt tccaacttc atgtcagat gttcacgga gtggtgaac accatgtcc gagttgtat ctacaaaat gagaaacgc tacaactac accactcct aaatctttc ctagaacag atcgccttg tattctaaa cttctttac gaaaagact aaaaatttg gaggacaat atcgaacgc ctcgagtct ggtttactc aaactggca tcaacttcg gaagaagtg gacggtttg aaagaagtg ttggcagtc caagaggta gaattgaat gaaaagaat gaagccgcc ggtaaactc attgccgtc ctaagtgcc gaaaacgaa aaagttgca aaagaacag gcaatcgct agcgaggaa gaagccaaa gttaaacta atcgaggag gatgttagt gtcaaacaa aaaatctgt gccgatgat ttggccaaa gccgagcca gcgctaatc gcagcccaa caagcttta aacaccctg aataaaaac aacttaact gaactgaaa tccttcacg caaccgcct gaagcggta gtcgctgta acagccgct gttttaatc ctgttttcg ccaaaagga cgaattccg aaagatcgt agctggaaa gcttgtaag gggttgatg acaaaagtc gataaattt ttagaagat ttaataaac tatgataag gaaaacatg caaccggaa gtagtgaaa gcaactctg gaatatacg aaaaatccc gagttcaat ccagaaaaa attttgagc aagtcggtg gcagctgct ggattgtgt gcgtgggtc attaatatt ttgaaattt tacgacgtg tttgtcgtg gtcgaaccc aagaggaaa gctttgcaa aaagccaat caggaactg gcagatgcg agagctaaa ctggccgag cttaacgag aaattgaat tatctcgag tcacaattg gcgattttg aaggccgat tttgaggaa gctacagcc gcgaagatg aaatgtcag gccgaagct gatgctacg actcaaatg atcgattta gctaatcga cttgttaat ggtttggcg tcggaaaat atcaggtgg cgagagttg gtcaagttg taccggaaa atgataatt acactgcca ggggatgtg ttgatgata acttgcttt atttcgtac gttggatgc ttcacgaga cggtacaga accgagatg gttgaacat cactggata ccctcgctg aaaaaaatc gagccgaaa attccgatg agtgaaggg ctggatccc ttatccctg ctcacagac gatgcaatg gtcgcagga tggaataat gaggggctt cctaatgat cgtatgtca accgagaat gcttgtatt ttaacaaat tcggcaaga tggcccctg atgattgat ccccaattg caaggagtt aagtggatc aaaactaaa tacggcgat agcttgaca gttattcga ctgagcatg aaaaattac ttggacact attgaaaga tgtatccaa aatggtgaa attatcctt gttgaaaac ataggcgag accgtggac gcggtctta gaccctgtt ctgggtcga gtcttagtc aaaaaaggc aaggcgata aaaatcggt gataaggaa gtggattac aaccccaac tttaggatg atcatgcac acgaaactg gcaaatccg cattacaaa ccggaaatt caagcacaa actaccctc atcaacttc acagtaacg cgagatggt ttagaggag cagcttttg gcagaagtc gtcaaagct gaacgccca gacttggaa agtttgaaa gcgaattta acgaaacaa caaaacgat ttcaaaatt attttgaaa agatgtgag gataattta ttgtttaga ctttcctcg gccacgggt aacatactg ggcgatgtg gaactagtc gagaatttg gaaacaacc aagaaaatg tccacggaa attgaagag aaagtcaaa gaagctaaa ataacttca gtcaaaatc gacgaagcg cgtgaacag taccgacca tgttcggct agagcttcg ctcctttat ttcattttg aacgatctt tgccgcatc aacgcaatc tatcaattc tcattgaag gccttcagt gtggtgttt aaggacgcg ttatctaaa gcagtaccg gccgaaaag ttaaaagat cgggtagtt aacttattg gacagtata actttttcg gtatttatg tacacaact cgaggtctg ttcgaatgt gacaagttg attttcatg gcccaaatg gcatttcaa attttgctg catgctaaa gaaatcgat cacgctgaa cttgacttt ctgttacgg taccctgta gtaccgaac ttgacaacc cctgtggac ttcctcaca aatcagtcc tggggcggc attaaagcc ctgtcagag tttgacgat ttcagaggt ttagacaaa gacttggaa ggctcggcg aaacgttgg cgaaaattc gtcgatagt gaatgcccc gaaaaagaa aagttcccc ggcgagtgg aaaaacaaa accgctttg caacacttg tgcatgatg cgtgccctc cgccccgac cgtatgacc tacgctgtt cgttgtttc attgaagag aaactcgga gccaaatac ataacagcc agatccgtc gaatttgca aaatcttac gaagaaaca agctcttca acccccgtg ttctttatt ctatcgcca ggcgtaaat ccgctcaag gatgtagaa aagctgggc aaaaagctg ggtttcacc tttgattct ggtaacttc cacagtatt tccttgggc caaggccag gaaatcgtg gctgaaaac gcaatggat ttggcgtcg gagcggggc gattgggtc attttgcaa aacattcat ttagtcgag cggtggttg tccactttg gagaaaaaa atggaacaa tgcgccgaa agcgcccat caaaagttt aggttgtat ttgagcgct gagcctaac gctgatccc caagtgtcc gtaatcccg caagggatt ctagaatcg tcgatcaaa atcacaaac gagccgccg actggcatg tatgccaat ttacacaaa gctttggac aatttcagc caggaaacg ttagacatg tgcacgaaa gaagcggag tttaagctg attttgttc gctttgtgc tatttccat gccgtggtt gccgaaaga cggaaattt gggccacaa gggtggaac aggaattac ccctttaat gttggcgat ctcacaatt agcgtaaac gtgttgtac aattatctg gaaagtaac agtaaggtc ccctacgaa gatttgcgc tatctgttt ggggaaata atgtacggg gggcatatt acggacgat tgggatcga cggttgtgc cgaaattat ctaatggag tatctccag cccgaattg ctggaagga gacttgcag tttgcgccg ggatttttt gccccacct aatacagac tatgttggg tatcatcgc tatattgac gaccgtttg ccgcctgaa agcccctac ctttacggc ctacaccca aacgcggaa attggcttc ttggcctac acttctgaa gttatgttt agaacaatt ttcgaaatg caaccgcga gatgcggga gcttcggta ggccatgga ttttctcga gaggataag gttaaatca acccttgaa gatatccaa gaccggctc cccgaggag tttccggtc gccgaaatg atggcaaaa gtcgaagac aggaccccg tacataatt gtggcgttc caggagtgt gacagaatg aacagttta acacgggag atgaagaga tcgcttcgg gagctcgac ttgggatta aagggtgaa cttacgatt acgtcggac atggaggtg ttggaagag gccctattt atggaccaa gtccctgac tcgtggtcc gtaaaggcc taccccagc atgctgcct ttgggtcag tgggtggcc gacttgggg gctagaatt aaggagctg gaggggtgg gttaccgat ttctcgatg cctcccacc gtctggctg gggggtttc ttcaacccc cagtcgttc ctcactgcc attatgcaa caaacggct cgcaaaaac gaatggcct ttggacaaa atgtgtctg ttgaccgac gtcaccaaa aagcagaaa gaggagttc ggggcggct cctcgggaa ggcgcatat gtgcacggt ttatttatg gagggcgca aggtgggat acgcagctc ggaagtatt gccgatgct aagcttaaa gaattgttc cctgtaatg ccggttata ttcatcaag gcaatcacg caggataag caggattta cggaatatg tacgactgt cctgtttac aaaacaagg actcgggga ccgactttc gtctggact tttaattta aaaacgaag gagaaggcg tccaagtgg acccttgca ggagtagcg attctgtta cagatttaa ttatttatt ttattgtag ctgttaata aaattttat tttaatt
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