Status
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Gene information
General information
TC008638 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 13501683..13509920
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008638
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01376

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008638 RA
      MFTGTVRIK ICEAQGLRP TDCSKRHNM TFGKVADDQ 
      LMDPYVTVD VDDTHVAQT TTKPKTLDP VWNEYFEQS 
      VDNAKTLTL TVFHDAAIP PDVFVANCS IPFEDLLNR 
      EKDEADFWV NLEPKGRLH VRIDLKLTT KDYHGAKPR 
      EFKERQGFN RRRGAMRRR VHQVNGHKF MATFLRQPT 
      FCSHCRDFI WGLGKQGYQ CQVCTCVVH KRCHLLVVT 
      KCPGMKDES SQQSTRFNV NVPHRFVVH NYKRFTFCD 
      HCGSLLYGL IRQGLQCEV CSLNVHKRC QKNVANNCG 
      INTKQMAEI LSAIGVSPD KQTPRRSKY VSGSGANGA 
      NFEGQSENQ APEDEMENT DERLTKMME EKMANNGGD 
      GAERKSKSK IGLEDFNFI KVLGKGSFG KVMLAEKKD 
      TDEVYAVKV LKKDVIIQD DDVDCTMTE KRILALAAK 
      HPFLTALHS CFQTKDRLF FVMEYVNGG DLMFQIQRA 
      RKFDEPRAR FYAAEVTLA LQFLHKHGV VYRDLKLDN 
      ILLDAEGHC KLADFGMCK EGILDGITT TTFCGTPDY 
      IAPEILQEL EYGASVDWW ALGVLMYEM MAGQPPFES 
      DNEDDLFES ILHDDVLYP VWLSKDAVS ILKGFMTKN 
      PSRRLGCVT AHGGEAAIR VHPFFRDID WNALEQRKV 
      KPPFKPKIK SKKDALNFD AEFTKEEPV LTPVSNEVL 
      HSINQEEFG GFSFINRDF NPSRFVAE
    CDS sequence
    • >TC008638 RA
      atgttcacg gggaccgtc cgcatcaaa atatgcgag 
      gctcagggc ttacggccg acggactgc tcgaagcgc 
      cacaacatg acgttcggg aaggtggcc gacgaccag 
      ctgatggac ccctacgtg accgtcgac gtcgacgac 
      actcacgtc gcccagacc accaccaag ccgaagacg 
      ctcgatccc gtctggaac gagtacttc gagcagagc 
      gtcgacaac gccaagacg ctcaccttg actgtcttt 
      cacgacgct gccatcccg cccgacgtc ttcgtcgcc 
      aattgcagc attcccttc gaggatctg ctcaatcgc 
      gagaaagac gaggccgac ttttgggta aatttagaa 
      cctaaaggc cgtcttcac gttagaata gacttgaag 
      ttgacaacg aaagattat catggagct aaacctcgc 
      gagttcaag gaacgccaa ggcttcaac cgaagacgg 
      ggtgccatg cggcggagg gtccaccaa gtcaatggt 
      cacaaattc atggcgacc ttcctccga cagcccacc 
      ttctgctcg cactgtcgc gattttatt tggggtttg 
      ggcaagcaa ggctatcaa tgtcaagtt tgcacctgc 
      gttgttcat aaacgatgc catcttcta gttgttaca 
      aagtgtcca ggaatgaaa gatgagtct agtcaacaa 
      agtacaaga tttaatgtg aatgtgccg caccgtttc 
      gtagtccac aattacaag agattcaca ttttgtgat 
      cattgtggt tcgttgttg tacggttta atccgccaa 
      ggactccag tgtgaagtt tgttcctta aacgtgcac 
      aaacgttgt caaaaaaat gtagcaaat aactgtggg 
      attaacacg aagcagatg gctgaaatt ctctcggcg 
      atcggtgtt tccccggat aaacaaacg cctagaagg 
      agtaaatac gtgtcagga tcaggggca aatggcgcg 
      aatttcgaa ggccagtcg gaaaatcag gccccggaa 
      gacgaaatg gaaaacact gacgaacgg ttaactaaa 
      atgatggag gagaaaatg gccaacaat gggggagac 
      ggcgccgaa cgtaaaagc aagtcgaag ataggttta 
      gaagacttc aacttcatc aaagtcctg ggcaagggg 
      agtttcggg aaagtgatg ctggcggag aagaaagac 
      accgatgaa gtatacgct gtgaaagta ttaaaaaag 
      gatgtgatt attcaagac gacgacgtc gattgcaca 
      atgaccgag aaacgtatt ctggctttg gccgccaaa 
      catcccttc ctaacagca ctccactcg tgctttcag 
      acaaaagat cggttattt tttgtcatg gagtacgta 
      aacggcggc gatttgatg ttccaaatc caaagggcg 
      cgaaaattt gacgagccg cgagccaga ttttacgcc 
      gctgaggtt actttagct ttacaattt ttgcacaaa 
      catggagtt gtttataga gatctcaag ttagataat 
      attttacta gacgctgaa gggcactgt aaactggcc 
      gatttcgga atgtgtaag gaagggatc ctggacgga 
      atcaccact acgaccttc tgcggcact cctgattac 
      atcgcgccc gaaattctt caagaactg gagtatggc 
      gcgagcgtg gactggtgg gctttgggc gtcttgatg 
      tacgagatg atggcagga caaccccct ttcgagtcc 
      gacaatgag gacgacttg ttcgagtct atcttgcac 
      gatgatgtg ctgtatccg gtctggttg agcaaagac 
      gctgttagt atattgaag ggcttcatg acgaagaac 
      cccagtcgg aggctgggc tgtgtgaca gcgcacggg 
      ggcgaggct gcgatcagg gtgcacccc ttttttaga 
      gacatcgac tggaacgcg ttagaacag agaaaagtg 
      aaaccaccc ttcaaacct aaaattaag agtaagaag 
      gacgccttg aactttgac gccgagttc acgaaggaa 
      gagccggtc ctgacgcca gtcagcaat gaagtgctg 
      cactctatc aaccaagaa gaattcgga ggtttctcc 
      tttatcaac agagacttc aatccttcg cgtttcgtc 
      gctgaataa
    mRNA sequence
    • >TC008638 RA
      gggctgaaa aataatact actccggtg ccccatagc 
      ggagagcac cattttttc tttgggaat cagtaaatt 
      gaggaaagt gtgaagtgt ttttaagca cacctcgta 
      cgcagagcg cgtctttgg acggtgggt gcatggtag 
      tcggtggcg tcatctgtg cttgggtcg atggtgcgg 
      ttgtttgtg gaggcgctg aagactgtg ggagaggtg 
      tctcctcgg tcgggaatt cgggagcgg cgtcgtcgt 
      cgctcgtcg tccatggct gctgtcaag aagtggtgg 
      tgtttgttt cgctccgtg tgcgtatgc agaagcagg 
      tgttgatgt ggtgtgtgg ccgagtgaa taagacggt 
      gaattcggt ggtggcgtc gtgcgttcc caatgttca 
      cggggaccg tccgcatca aaatatgcg aggctcagg 
      gcttacggc cgacggact gctcgaagc gccacaaca 
      tgacgttcg ggaaggtgg ccgacgacc agctgatgg 
      acccctacg tgaccgtcg acgtcgacg acactcacg 
      tcgcccaga ccaccacca agccgaaga cgctcgatc 
      ccgtctgga acgagtact tcgagcaga gcgtcgaca 
      acgccaaga cgctcacct tgactgtct ttcacgacg 
      ctgccatcc cgcccgacg tcttcgtcg ccaattgca 
      gcattccct tcgaggatc tgctcaatc gcgagaaag 
      acgaggccg acttttggg taaatttag aacctaaag 
      gccgtcttc acgttagaa tagacttga agttgacaa 
      cgaaagatt atcatggag ctaaacctc gcgagttca 
      aggaacgcc aaggcttca accgaagac ggggtgcca 
      tgcggcgga gggtccacc aagtcaatg gtcacaaat 
      tcatggcga ccttcctcc gacagccca ccttctgct 
      cgcactgtc gcgatttta tttggggtt tgggcaagc 
      aaggctatc aatgtcaag tttgcacct gcgttgttc 
      ataaacgat gccatcttc tagttgtta caaagtgtc 
      caggaatga aagatgagt ctagtcaac aaagtacaa 
      gatttaatg tgaatgtgc cgcaccgtt tcgtagtcc 
      acaattaca agagattca cattttgtg atcattgtg 
      gttcgttgt tgtacggtt taatccgcc aaggactcc 
      agtgtgaag tttgttcct taaacgtgc acaaacgtt 
      gtcaaaaaa atgtagcaa ataactgtg ggattaaca 
      cgaagcaga tggctgaaa ttctctcgg cgatcggtg 
      tttccccgg ataaacaaa cgcctagaa ggagtaaat 
      acgtgtcag gatcagggg caaatggcg cgaatttcg 
      aaggccagt cggaaaatc aggccccgg aagacgaaa 
      tggaaaaca ctgacgaac ggttaacta aaatgatgg 
      aggagaaaa tggccaaca atgggggag acggcgccg 
      aacgtaaaa gcaagtcga agataggtt tagaagact 
      tcaacttca tcaaagtcc tgggcaagg ggagtttcg 
      ggaaagtga tgctggcgg agaagaaag acaccgatg 
      aagtatacg ctgtgaaag tattaaaaa aggatgtga 
      ttattcaag acgacgacg tcgattgca caatgaccg 
      agaaacgta ttctggctt tggccgcca aacatccct 
      tcctaacag cactccact cgtgctttc agacaaaag 
      atcggttat tttttgtca tggagtacg taaacggcg 
      gcgatttga tgttccaaa tccaaaggg cgcgaaaat 
      ttgacgagc cgcgagcca gattttacg ccgctgagg 
      ttactttag ctttacaat ttttgcaca aacatggag 
      ttgtttata gagatctca agttagata atattttac 
      tagacgctg aagggcact gtaaactgg ccgatttcg 
      gaatgtgta aggaaggga tcctggacg gaatcacca 
      ctacgacct tctgcggca ctcctgatt acatcgcgc 
      ccgaaattc ttcaagaac tggagtatg gcgcgagcg 
      tggactggt gggctttgg gcgtcttga tgtacgaga 
      tgatggcag gacaacccc ctttcgagt ccgacaatg 
      aggacgact tgttcgagt ctatcttgc acgatgatg 
      tgctgtatc cggtctggt tgagcaaag acgctgtta 
      gtatattga agggcttca tgacgaaga accccagtc 
      ggaggctgg gctgtgtga cagcgcacg ggggcgagg 
      ctgcgatca gggtgcacc cctttttta gagacatcg 
      actggaacg cgttagaac agagaaaag tgaaaccac 
      ccttcaaac ctaaaatta agagtaaga aggacgcct 
      tgaactttg acgccgagt tcacgaagg aagagccgg 
      tcctgacgc cagtcagca atgaagtgc tgcactcta 
      tcaaccaag aagaattcg gaggtttct cctttatca 
      acagagact tcaatcctt cgcgtttcg tcgctgaat 
      aactttacc acatccatt tatcctttc ttgttgtcc 
      ccttagttg aaataacca cgactcggg taaaccaga 
      agcttgaaa gctttaatt ttgtaaaaa ttgaggagg 
      aaatgcgct agtactcat tatccaatg ttattatta 
      tgatgttta ttttatgga acagagttt atttaaatt 
      tgatctgat taagtttgt aattgttaa ggaagacaa 
      agtatcagt ggaccccaa gtcactgca gctaaatta 
      tcagaattt tgcgtatcg tttaggggt agttcgaat 
      attgtgaag tggggttac ttggagttt ctgtgattt 
      aacgctgag acgccagat ttacttgtc ttaactcta 
      gtcactgaa tggcctatg ctcctaata tcaaagagt 
      aaacacgca agtcggtcg aggttggcc actataatg 
      ttaagtatt tgtaatttt aattaatgc actttctgc 
      aggagcctt attgcagtt cgttcttcc ccggccgta 
      cttgctttc acgaggact cgttcatac ggtatcgtc 
      attatttga aattatttt tcgtcttgt gatattgtg 
      ggaatttgc aatatattt agtgtaggc cgttgtgcc 
      taattttgt ttacagttt tttaaagag ttcaaagga 
      tttcgcttg gacggcgac ccgcactag acagaaata 
      taatcactt tcaacgtaa cacacacac gttgatata 
      cgtgttagg gaaatgtgc attatttta agagagctt 
      acgatttac caaagaatt ttatttatt actttttaa 
      cgagatacg tgtgtaaat gttaataat tatgatagt 
      ttacgtagg agaataatt tattttgtt ggctgcgtg 
      cgagcggat ttcgcattg tctcaggag tggtctctg 
      caagatttg agtgtgtgc gagcccccc aacacatag 
      ttgagagtt tatcacaaa cgacacaaa gtgttggtt 
      tgagtttta tctgccata taactaagc tatctagag 
      aacattcag ctacatttc gaggaaatg tacatattg 
      ttaaaagta aaagaaaag tatataacg ttttagata 
      tttattaat gtggagctt agagtagta ggtaaatta 
      ataaaatta acccatgct aattttagt gtatgtcta 
      gataatatc tatatcaca ggtctgtgt tagcaccga 
      gctgttcaa gaagtacct acttgaggt tggcagtgt 
      agagttatt tagttctct agtagagcc tcagtctct 
      cactcgttc gttcggttt ttgccgtga aggcgcgtg 
      cttgcatac ggtgttgtg ccaaaccat gtccaccct 
      aacccgcag cctttccgc acccccacc actgtctaa 
      accccactg tcgcattgt tattgttta tatcatatt 
      tgtactagc aatgtgttg taaatggtg tacctattt 
      ctctgacgg tgtactaaa aattataaa taaacatta 
      tttaatgta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY