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Gene information
General information
TC008615 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 16083298..16106081
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008615
    iBeetle screen iB_01373

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008615 RA
      MRLRRRKRP EESAWRAVR RCVVFFTTI CIVPVLLVL 
      IVTTDSYMR PVKQTPYHR PQFDSVEHL EPNLQNLTG 
      VEKRLERRR LHLQKACKL LGLNVPGND SLHKPNPWE 
      FLIDTKHNL VWCNVFKAA STSWMYNFN ILAGYSPYF 
      LKKSKVVPL SLARQKYPR PSLPFLKKA FNNSVSFLI 
      TRHPLERLL SAYRDKLMF ALPHTHHRK LGNEIIVKY 
      RAKVKHQKG FKAEKWPTF PEFVLYLLD CVKQSQTLD 
      MHWTPIAEF CTPCMFNFD IIAHTETLQ EDQQYIIKK 
      AHLEGLVEP QWRNAGHGT TARQVRKYY SQLTRSQIL 
      QLYHIYRYD FELFNYSLK GYLELGQLD DDPSTLLAA 
      ITLKDFPQS KNKPRLY
    CDS sequence
    • >TC008615 RA
      atgaggttg cggaggcgc aagagaccg gaagagagc 
      gcgtggagg gccgttcgc aggtgcgtc gtcttcttc 
      accaccatc tgcattgtg ccggtgctg ttggtgctc 
      attgtcacc acagatagc tacatgaga ccggtcaaa 
      caaactccc tatcacagg ccgcagttc gacagcgtg 
      gagcacctc gagcccaac ctccagaac ctaactggc 
      gtggaaaag cgcctcgaa cgtcgccgc ctacacttg 
      caaaaagct tgtaaatta ctgggtttg aacgttcct 
      ggcaatgat tcccttcac aaacccaac ccttgggaa 
      tttctcatc gacaccaaa cacaatctc gtgtggtgc 
      aatgttttc aaagctgcg agcacctcg tggatgtac 
      aacttcaac attcttgca gggtactct ccatatttt 
      ctcaaaaag agtaaagtg gtgcctctt agcttggcc 
      cggcagaag tatcctcgg ccgtctctt ccgttttta 
      aaaaaagcg ttcaataat tcagtttca tttttaata 
      acacgacat ccactggag agactctta tcagcctac 
      agagacaag ctaatgttc gctctgcct cacacgcac 
      cacagaaaa ttggggaac gagatcatc gttaaatac 
      cgagcgaaa gtgaagcac caaaagggg ttcaaagcc 
      gagaagtgg cccacattt cctgaattt gtgctttac 
      ctcctcgat tgcgtcaag caaagtcaa actctggac 
      atgcactgg actcctata gctgaattt tgcacaccc 
      tgtatgttc aatttcgac ataatcgcg catactgag 
      acacttcaa gaagaccaa cagtacatc atcaagaag 
      gcgcatctt gaaggattg gtggaaccc caatggagg 
      aacgcaggc catgggaca accgctaga caagtccgg 
      aaatactac tcccaatta acgcggtcg caaatttta 
      caattgtat cacatatat agatacgac tttgaattg 
      ttcaattac tcgctgaaa ggctactta gagttagga 
      caacttgac gatgatccg tctactcta ctcgcggcc 
      atcacactc aaggatttt cctcagagt aaaaacaag 
      ccgcgactc tattga
    mRNA sequence
    • >TC008615 RA
      gtacaactg cagtgcggt tcaaaacca cggtcatgt 
      tggagcaga agtgaggct tgttccgac atgacaccg 
      ccaaaatcc ggtgtgtga taaccgaaa aacctaacc 
      aattcaatc acgagtctt ccactaaac catgtgaat 
      aattatcaa aatccgagc cgacaaatc agatagatc 
      ctagtaaaa tgaatggag cgttcatat catgaggtt 
      gcggaggcg caagagacc ggaagagag cgcgtggag 
      ggccgttcg caggtgcgt cgtcttctt caccaccat 
      ctgcattgt gccggtgct gttggtgct cattgtcac 
      cacagatag ctacatgag accggtcaa acaaactcc 
      ctatcacag gccgcagtt cgacagcgt ggagcacct 
      cgagcccaa cctccagaa cctaactgg cgtggaaaa 
      gcgcctcga acgtcgccg cctacactt gcaaaaagc 
      ttgtaaatt actgggttt gaacgttcc tggcaatga 
      ttcccttca caaacccaa cccttggga atttctcat 
      cgacaccaa acacaatct cgtgtggtg caatgtttt 
      caaagctgc gagcacctc gtggatgta caacttcaa 
      cattcttgc agggtactc tccatattt tctcaaaaa 
      gagtaaagt ggtgcctct tagcttggc ccggcagaa 
      gtatcctcg gccgtctct tccgttttt aaaaaaagc 
      gttcaataa ttcagtttc atttttaat aacacgaca 
      tccactgga gagactctt atcagccta cagagacaa 
      gctaatgtt cgctctgcc tcacacgca ccacagaaa 
      attggggaa cgagatcat cgttaaata ccgagcgaa 
      agtgaagca ccaaaaggg gttcaaagc cgagaagtg 
      gcccacatt tcctgaatt tgtgcttta cctcctcga 
      ttgcgtcaa gcaaagtca aactctgga catgcactg 
      gactcctat agctgaatt ttgcacacc ctgtatgtt 
      caatttcga cataatcgc gcatactga gacacttca 
      agaagacca acagtacat catcaagaa ggcgcatct 
      tgaaggatt ggtggaacc ccaatggag gaacgcagg 
      ccatgggac aaccgctag acaagtccg gaaatacta 
      ctcccaatt aacgcggtc gcaaatttt acaattgta 
      tcacatata tagatacga ctttgaatt gttcaatta 
      ctcgctgaa aggctactt agagttagg acaacttga 
      cgatgatcc gtctactct actcgcggc catcacact 
      caaggattt tcctcagag taaaaacaa gccgcgact 
      ctattgagc attttttgc aatactttg agattgtga 
      tacatagac atacgttta cgttgtttt taacttgtt 
      atcaattct tggcattag cactgttat attgtgata 
      ggtaggatg ttactacac cttaaatat agatttgct 
      ttattttgg tacttacga ttcgctttc gttcttcac 
      ttcgaaccc caacacgta tttcccgtt tatcgattt 
      cttccaaac tttcacaaa ttttacaaa attttggct 
      gtacttctt tgtaatttc tcgtcgcaa atttgatgc 
      tatttcttg acggtgttg taaagatga ctttaagtg 
      agcattcac tgatttaac ttcatccaa ttttgaacg 
      aagaaatgc actgattcc atccgagca atcgaattc 
      taaatcaat atccatcat ctcaaatga acatacagg 
      ttaacacta ggtatctaa agctgattt gtaagttat 
      ccgtccact ttctgaaac aaacataaa taagtaaac 
      taatttcgc tttttccga taaacataa aattattaa 
      ttacgtttt atcgtaagt gtactaaat aaaaaagtg 
      gtcacaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY