Status
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Gene information
General information
TC033883
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008608
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 15923937..15931809
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033883
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01372

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands colour darker - Number of animals showing the phenotype: 4 (41 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033883 RA
      MGTPLIGVR CYPIEEYPF YHCEGCPPG STGNGTNCH 
      DLDECDLAQ PCDENVRCT NLSPGYRCD PCPPGFTGS 
      RGSQGVGLE EARRNKQRC EDIDECRQA APCGQNAYC 
      VNTVGSYDC VECANGRYD PSIGSCLGE NYCPGGIQC 
      DANAKCVQH GPNSYSCEC KTGWAGNGL YCGPDHDLD 
      GWSDQRLPC RDPRCKLDN CLDVPNSGQ EDSDGDGVG 
      DACDNDADK DRIPNDKDN CPFVPNFDQ KDSDGDRVG 
      DACDNCPYD SNPDQKDTD HDRKGDRCD DDIDNDGIR 
      NERDNCPLI ANRDQSDRD HDGIGDVCD NCPHNYNPK 
      QKDSDFDNI GDECDVGPD QDHDGIPDQ IDNCPTVPN 
      GNQLDTDGD NIGDACDDD KDNDGHKDH QDNCYLVYN 
      PDQLDSDGD GRGDACQCD TDNDTRCDN VDNCPNNSL 
      IYQTDFSKY QTVALDPEG SSQMDPVWE IYNQGAEIT 
      QTVNSDPGL AVGNDKFDG VDFEGTFYV GTDVDDDYA 
      GFIFSYQSN KQFYVVMWK QNQQTYWDS NPFRAVGEA 
      GIQIKLVDS QTGPGKMLR NSLWHTGDT PNQVKLLWK 
      DARNMGWRP KTSYRWYLI HRPRIGLIR LQIYEKDKK 
      ITDSGNIFD RTLHGGRLG VFCFSQETI VWSDLAYRC 
      NERLPESMW NELPNNLRG SINKDFNRA HRISSGNPF 
      LQ
    CDS sequence
    • >TC033883 RA
      atgggcact ccacttata ggagtgaga tgttatccc 
      atcgaagaa tatcccttt taccattgc gaaggttgt 
      cctccagga tccaccgga aatggaacg aactgtcat 
      gacctggac gagtgcgac ttggctcaa ccttgtgac 
      gaaaatgta cggtgtaca aacctctcg cctggctat 
      aggtgcgac ccttgccct cctgggttc accggaagt 
      cgcggaagc caaggagtg ggcttagag gaagcccga 
      cggaacaag caacgctgt gaagacatc gatgagtgc 
      cgccaagca gccccttgc gggcagaac gcctactgc 
      gtcaacact gtgggttcc tacgactgt gtggagtgt 
      gccaacggg cgatacgac cctagcata ggatcatgt 
      cttggggaa aactactgt cctggcggc atccaatgc 
      gatgcaaac gctaaatgt gtccaacac ggcccgaat 
      tcctactct tgcgagtgt aagactgga tgggcagga 
      aatgggtta tattgtggg cctgaccat gatcttgac 
      ggatggtca gatcagcga ttaccttgt cgtgaccca 
      agatgcaag cttgataat tgtttggat gttccaaat 
      tctggacag gaagacagt gatggtgat ggggtagga 
      gatgcgtgt gacaatgat gcagataaa gacaggatt 
      cccaacgat aaggataac tgtcctttt gtccctaat 
      ttcgatcag aaggattca gatggggat agagtcggg 
      gatgcgtgt gataattgt ccttatgac tcgaatccg 
      gatcagaag gatacggat catgacaga aagggtgac 
      cgttgtgac gatgatatt gataatgac gggataagg 
      aatgagagg gataattgc ccattgatt gctaacagg 
      gatcaaagc gatagagat catgacggt attggggat 
      gtttgtgat aattgtcct cataactat aatcccaaa 
      caaaaggat agtgatttc gataacatt ggagatgag 
      tgtgatgta ggccctgat caggatcat gatggtatc 
      cccgaccaa attgacaac tgtccaaca gttcccaat 
      ggaaatcaa ttggataca gatggggac aatattgga 
      gatgcctgt gatgacgat aaagataat gacgggcac 
      aaggaccat caagataac tgttatcta gtttacaac 
      ccagatcaa ctcgattct gatggcgac ggtcgcggc 
      gacgcctgt caatgcgac actgacaac gatactcgc 
      tgcgacaat gtcgacaac tgtcccaac aactccctc 
      atctatcaa acggacttt agcaaatac caaacggtg 
      gcccttgac ccggaaggc tcttcccaa atggaccca 
      gtctgggag atctacaac caaggagcc gaaatcact 
      cagaccgta aacagcgac cctggtctc gccgtcggc 
      aacgacaag ttcgacgga gttgacttc gaaggcacc 
      ttttacgta ggcaccgat gtcgacgac gattacgcc 
      ggttttata ttcagttat cagagcaat aaacaattc 
      tatgtcgtg atgtggaag caaaaccaa cagacttac 
      tgggacagc aaccctttc cgtgctgtt ggagaagca 
      ggtattcag ataaaacta gtcgattcg cagaccggt 
      cctggaaaa atgcttcga aattcctta tggcacacg 
      ggagatact ccaaatcaa gtgaaattg ttgtggaag 
      gatgcaagg aatatggga tggcgaccg aaaacgtcg 
      taccgatgg tacctcatc cacaggccc agaattgga 
      ttaatccga ttgcagatt tacgaaaag gataagaaa 
      attacagat tcggggaat atttttgac aggactctt 
      cacggtgga cgactggga gtgttctgc ttctcacaa 
      gaaacgatc gtttggtct gacttggcc tacaggtgt 
      aacgaacgt ttgcctgaa tccatgtgg aacgaattg 
      cccaacaat ctgagaggg tcgatcaac aaagatttt 
      aacagagca catcgcatc tcttcggga aatccgttc 
      ttacaatag
    mRNA sequence
    • >TC033883 RA
      ttccacttt atagaacta ctgataggc attaagaca 
      cacaccttg cacagtaaa gaactatta tttcaataa 
      ctgagaaaa caaattttg tttaatttt gtcgactcc 
      cacttgtta tagatgtaa gaaaggcac ttttaggaa 
      acagttgtt tatattgtc cagcggtaa agcacacgt 
      tttcagaaa attaatata cgagtatgt atgttacat 
      gtgaaaaat attagttta actaataat aatgtaaac 
      taatgatgt tgtcccttt tagtcgtaa ggtcattaa 
      tgtagactt aatttttcc gacaagatt ccaaaaagt 
      ttgccgaat agtttagct ttccttgtc aatttgtgc 
      gtatttaac atgtgacta aagctacag taagacgtg 
      gttcttgtc aaaactact aaaaacgct acagtggga 
      cgtagttag agcatctgg ctgctgatt ggttcagtt 
      ttttgtatt ccagggttc tgtagatgt tattagctg 
      cgtgtgatg tgaaatacg attaattct atgtaataa 
      tcctgtatg tcttaaata tagttgatt taaacaaaa 
      gtttttgtt ttatttgtt tttcttttt attagggtg 
      cgtgccaat ttggactca ccaaaatcc acaatccag 
      tcccagagt tacgacacc ttacgccag atacccgtt 
      ttactaaat gaactacat tatatatgt atcgaagca 
      cgaattaac cctcccaaa atttttatt caaaaaagt 
      ggtaactca cacacaacg caccaaaaa gacttagta 
      ttattttag catagtttt ttatttctt attattata 
      taatatttg gaatgtcta tatattttt ttaataaat 
      ttagctaaa aatgagtga ttgtcgtta ttcatgggc 
      actccactt ataggagtg agatgttat cccatcgaa 
      gaatatccc ttttaccat tgcgaaggt tgtcctcca 
      ggatccacc ggaaatgga acgaactgt catgacctg 
      gacgagtgc gacttggct caaccttgt gacgaaaat 
      gtacggtgt acaaacctc tcgcctggc tataggtgc 
      gacccttgc cctcctggg ttcaccgga agtcgcgga 
      agccaagga gtgggctta gaggaagcc cgacggaac 
      aagcaacgc tgtgaagac atcgatgag tgccgccaa 
      gcagcccct tgcgggcag aacgcctac tgcgtcaac 
      actgtgggt tcctacgac tgtgtggag tgtgccaac 
      gggcgatac gaccctagc ataggatca tgtcttggg 
      gaaaactac tgtcctggc ggcatccaa tgcgatgca 
      aacgctaaa tgtgtccaa cacggcccg aattcctac 
      tcttgcgag tgtaagact ggatgggca ggaaatggg 
      ttatattgt gggcctgac catgatctt gacggatgg 
      tcagatcag cgattacct tgtcgtgac ccaagatgc 
      aagcttgat aattgtttg gatgttcca aattctgga 
      caggaagac agtgatggt gatggggta ggagatgcg 
      tgtgacaat gatgcagat aaagacagg attcccaac 
      gataaggat aactgtcct tttgtccct aatttcgat 
      cagaaggat tcagatggg gatagagtc ggggatgcg 
      tgtgataat tgtccttat gactcgaat ccggatcag 
      aaggatacg gatcatgac agaaagggt gaccgttgt 
      gacgatgat attgataat gacgggata aggaatgag 
      agggataat tgcccattg attgctaac agggatcaa 
      agcgataga gatcatgac ggtattggg gatgtttgt 
      gataattgt cctcataac tataatccc aaacaaaag 
      gatagtgat ttcgataac attggagat gagtgtgat 
      gtaggccct gatcaggat catgatggt atccccgac 
      caaattgac aactgtcca acagttccc aatggaaat 
      caattggat acagatggg gacaatatt ggagatgcc 
      tgtgatgac gataaagat aatgacggg cacaaggac 
      catcaagat aactgttat ctagtttac aacccagat 
      caactcgat tctgatggc gacggtcgc ggcgacgcc 
      tgtcaatgc gacactgac aacgatact cgctgcgac 
      aatgtcgac aactgtccc aacaactcc ctcatctat 
      caaacggac tttagcaaa taccaaacg gtggccctt 
      gacccggaa ggctcttcc caaatggac ccagtctgg 
      gagatctac aaccaagga gccgaaatc actcagacc 
      gtaaacagc gaccctggt ctcgccgtc ggcaacgac 
      aagttcgac ggagttgac ttcgaaggc accttttac 
      gtaggcacc gatgtcgac gacgattac gccggtttt 
      atattcagt tatcagagc aataaacaa ttctatgtc 
      gtgatgtgg aagcaaaac caacagact tactgggac 
      agcaaccct ttccgtgct gttggagaa gcaggtatt 
      cagataaaa ctagtcgat tcgcagacc ggtcctgga 
      aaaatgctt cgaaattcc ttatggcac acgggagat 
      actccaaat caagtgaaa ttgttgtgg aaggatgca 
      aggaatatg ggatggcga ccgaaaacg tcgtaccga 
      tggtacctc atccacagg cccagaatt ggattaatc 
      cgattgcag atttacgaa aaggataag aaaattaca 
      gattcgggg aatattttt gacaggact cttcacggt 
      ggacgactg ggagtgttc tgcttctca caagaaacg 
      atcgtttgg tctgacttg gcctacagg tgtaacgaa 
      cgtttgcct gaatccatg tggaacgaa ttgcccaac 
      aatctgaga gggtcgatc aacaaagat tttaacaga 
      gcacatcgc atctcttcg ggaaatccg ttcttacaa 
      tagtcaaat tactggtgt taaccacac tttttgtgt 
      tcttctaga ttgaaactt tgccatttt gtgtactta 
      ccatgtttt aaataacgt taatgtgtt gacagtgtg 
      aatctttga caataaagt tatatatta aca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY