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Gene information
General information
TC034816 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid50: 248016..253730
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034816
    iBeetle screen iB_01370
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034816 RA
      MKQASNEKS SVSELKPWP SFIQTRLDI WDKLKAEKE 
      AEIASKPLQ GIKVTLPDG KVVEGVAWK TTAYDIAKG 
      ISQGLADNC VISKVNGVL WDLDRPLEG DCKLELLKF 
      DNPEAQSVF WHSTAHILG EALERVYGG CLCYGPPIE 
      SGFYYDMFL EDKGISAGD FPYLENLMK QITKEKQPF 
      ERLEMKKED LLEMFKYNP FKVRILNEK VDTPTTTVY 
      RCGPLIDLC RGPHVRHTG KIKALKVTK NSSTYWEGK 
      ADAETLQRV YGISFPDNK QLKEWEKFQ EEAAKRDHR 
      KIGRDQELF FFHELSPGS CFFQPRGAH IYNTLINFI 
      KKEYRKRGF QEVISPNIF NAKLWQVSG HWAHYADNM 
      FSFDVEKDK FALKPMNCP GHCLIFDNR TRSWRELPL 
      RLADFGVLH RNELSGALT GLTRVRRFQ QDDAHIFCA 
      LEHIKQEIK GALDFLSHV YSVFGFTFN VVLSTRPEK 
      YLGDLAMWE QAEKALSEG LDDFGAPWS VNPGDGAFY 
      GPKIDITVH DALKRAHQC GTIQLDFQL PIRFNLSYV 
      SESGEKKRP VIIHRAILG SVERMIAIL TESCGGKWP 
      FWISPRQAM VIPVGPTYD EYAQDVKKK LYEAGFMAE 
      VDTDAGDTM NKKIRNAQL AQFNFILVV GEKERSSQT 
      VNVRTRDNI VHGEISVEG LIEKFKVLQ ENYTRAEDS 
      F
    CDS sequence
    • >TC034816 RA
      atgaaacag gcaagtaac gaaaagtct tcagtgagt 
      gaactgaaa ccttggccc tcgtttatc caaactcgc 
      ttggacatt tgggataag ctcaaagct gaaaaagaa 
      gcggaaatt gcaagtaag cccctacaa ggcatcaaa 
      gtcacactg cctgatggt aaggtggtt gagggtgtt 
      gcatggaag accacagct tatgatatt gcaaaaggg 
      atcagtcaa gggctggct gataattgc gtgatttcg 
      aaagttaat ggtgttttg tgggacttg gatcgacct 
      ttggaagga gattgcaag ttggaacta ttgaaattt 
      gataatcct gaagcccag tcagtgttt tggcactca 
      acggctcat attctgggg gaagccctt gaaagggtg 
      tacgggggg tgtctctgt tatggccct ccgatcgag 
      tcaggattt tactatgac atgttttta gaagataag 
      gggatttcg gctggtgat tttccctac ttggaaaat 
      ctgatgaaa caaatcacg aaggaaaaa caaccattt 
      gaacgtttg gaaatgaag aaagaagat ttattggaa 
      atgttcaaa tacaatcct tttaaagtg aggatttta 
      aatgaaaaa gtggacaca cctactact actgtatat 
      cgctgtgga cctctaatt gatctgtgt agaggcccc 
      catgtaaga cacaccgga aaaattaag gctttaaaa 
      gttaccaaa aattcttca acttattgg gaaggtaaa 
      gccgatgct gaaacttta caaagggtg tatggtatc 
      agttttccc gacaataaa caattgaag gaatgggaa 
      aaattccaa gaagaggct gcaaaacgc gatcatcgg 
      aaaatcgga cgagaccaa gaattattc tttttccac 
      gaactctct cctggctca tgctttttc caacctcgt 
      ggcgctcac atctacaac accttgata aatttcata 
      aagaaagag tatcggaaa cgtggcttc caggaagta 
      atttctcct aacatcttt aacgcgaaa ttgtggcaa 
      gtttccggt cattgggct cattatgct gacaatatg 
      ttttctttc gacgtcgag aaagataaa tttgcttta 
      aaaccgatg aactgtccc ggccattgt ttgattttc 
      gataaccgt acgcgttcc tggcgcgag ctgcctctt 
      cgtctcgct gatttcggt gttttgcac cgtaatgag 
      ctctcagga gcccttacc ggcttaact cgtgtccgt 
      cgtttccaa caagacgac gctcacatt ttctgtgcc 
      ctcgagcac atcaagcag gagattaaa ggcgctttg 
      gatttcctg agccacgtt tattcagtt tttggtttc 
      accttcaac gtagttttg tccacaaga ccggagaaa 
      tatctcgga gacttggca atgtgggag caagcggaa 
      aaagccctc agcgagggt ttggacgat tttggggct 
      ccctggtcg gtgaatccc ggcgatggg gctttttac 
      ggccccaaa atcgacatt acggtccac gatgcgctc 
      aaaagggca catcagtgt ggtactatt caactggat 
      ttccaacta ccaatcagg tttaatctg tcgtatgtg 
      agcgagagt ggggaaaag aagagacct gtaattatc 
      catcgggct attttggga tcggtggag aggatgatt 
      gcgattttg actgaatcg tgtgggggg aaatggccg 
      ttttggata tcgccgaga caggcgatg gtaattcca 
      gtggggcca acttatgat gagtacgcg caggatgtg 
      aagaagaaa ttgtatgag gctggtttt atggccgag 
      gtggatacg gatgcgggg gatacgatg aataagaaa 
      attagaaat gcacagttg gcacaattt aattttatt 
      ttggttgtt ggagagaag gagagaagt agtcaaacg 
      gtcaacgtg agaactcga gataacatc gtccatggg 
      gaaatatct gttgaagga ttaattgaa aagtttaaa 
      gtgttgcaa gagaattat acacgagct gaggatagt 
      ttttaa
    mRNA sequence
    • >TC034816 RA
      tggttgaaa atctcgaaa atataaata tcgacgaaa 
      aggcgacgt tgaagaaaa tgaaacagg caagtaacg 
      aaaagtctt cagtgagtg aactgaaac cttggccct 
      cgtttatcc aaactcgct tggacattt gggataagc 
      tcaaagctg aaaaagaag cggaaattg caagtaagc 
      ccctacaag gcatcaaag tcacactgc ctgatggta 
      aggtggttg agggtgttg catggaaga ccacagctt 
      atgatattg caaaaggga tcagtcaag ggctggctg 
      ataattgcg tgatttcga aagttaatg gtgttttgt 
      gggacttgg atcgacctt tggaaggag attgcaagt 
      tggaactat tgaaatttg ataatcctg aagcccagt 
      cagtgtttt ggcactcaa cggctcata ttctggggg 
      aagcccttg aaagggtgt acggggggt gtctctgtt 
      atggccctc cgatcgagt caggatttt actatgaca 
      tgtttttag aagataagg ggatttcgg ctggtgatt 
      ttccctact tggaaaatc tgatgaaac aaatcacga 
      aggaaaaac aaccatttg aacgtttgg aaatgaaga 
      aagaagatt tattggaaa tgttcaaat acaatcctt 
      ttaaagtga ggattttaa atgaaaaag tggacacac 
      ctactacta ctgtatatc gctgtggac ctctaattg 
      atctgtgta gaggccccc atgtaagac acaccggaa 
      aaattaagg ctttaaaag ttaccaaaa attcttcaa 
      cttattggg aaggtaaag ccgatgctg aaactttac 
      aaagggtgt atggtatca gttttcccg acaataaac 
      aattgaagg aatgggaaa aattccaag aagaggctg 
      caaaacgcg atcatcgga aaatcggac gagaccaag 
      aattattct ttttccacg aactctctc ctggctcat 
      gctttttcc aacctcgtg gcgctcaca tctacaaca 
      ccttgataa atttcataa agaaagagt atcggaaac 
      gtggcttcc aggaagtaa tttctccta acatcttta 
      acgcgaaat tgtggcaag tttccggtc attgggctc 
      attatgctg acaatatgt tttctttcg acgtcgaga 
      aagataaat ttgctttaa aaccgatga actgtcccg 
      gccattgtt tgattttcg ataaccgta cgcgttcct 
      ggcgcgagc tgcctcttc gtctcgctg atttcggtg 
      ttttgcacc gtaatgagc tctcaggag cccttaccg 
      gcttaactc gtgtccgtc gtttccaac aagacgacg 
      ctcacattt tctgtgccc tcgagcaca tcaagcagg 
      agattaaag gcgctttgg atttcctga gccacgttt 
      attcagttt ttggtttca ccttcaacg tagttttgt 
      ccacaagac cggagaaat atctcggag acttggcaa 
      tgtgggagc aagcggaaa aagccctca gcgagggtt 
      tggacgatt ttggggctc cctggtcgg tgaatcccg 
      gcgatgggg ctttttacg gccccaaaa tcgacatta 
      cggtccacg atgcgctca aaagggcac atcagtgtg 
      gtactattc aactggatt tccaactac caatcaggt 
      ttaatctgt cgtatgtga gcgagagtg gggaaaaga 
      agagacctg taattatcc atcgggcta ttttgggat 
      cggtggaga ggatgattg cgattttga ctgaatcgt 
      gtgggggga aatggccgt tttggatat cgccgagac 
      aggcgatgg taattccag tggggccaa cttatgatg 
      agtacgcgc aggatgtga agaagaaat tgtatgagg 
      ctggtttta tggccgagg tggatacgg atgcggggg 
      atacgatga ataagaaaa ttagaaatg cacagttgg 
      cacaattta attttattt tggttgttg gagagaagg 
      agagaagta gtcaaacgg tcaacgtga gaactcgag 
      ataacatcg tccatgggg aaatatctg ttgaaggat 
      taattgaaa agtttaaag tgttgcaag agaattata 
      cacgagctg aggatagtt tttaagctt gtcattcct 
      tagctgtat tatttattt taaattatg aaaagtacc 
      taccgtaga tttttgtaa tttcgtttg aaatgaata 
      aagaaattt gaattcgaa gtgatttta atgagagtc 
      tctttaata tacatatta tattgtcgg caaaaagta 
      acataccat gaaatggtt tcgtcaata ttacattac 
      tataataat aaaaattct ctagcaata acaatctac 
      tagatacac cattaacaa tcttaatct ctggtattt 
      atgtcttaa aaatgactt tacaaaaag aaaagttca 
      acaaatgca aaatacact tggcgtctt taatgtggt 
      ttgtgtggc ttgaaatta aagtagcga cattaaaga 
      cccaaaaat atttctttt tgccatctg tcttacaat 
      taaagatct acaaaaagt acattcttc ctatttatt 
      ttacagtga ataagtata ttggtggca tcggtttga 
      agagattgt ataatgttt acagactag ttacaaatt 
      aatcactcc tttatttat gatgaatat tacaaaata 
      cttcattgc tctcgaact cataagtgc aaacttctg 
      aatcaacag gaatatgtt atatttatt caacaacga 
      caactcaag aaaatggcg acgaaaata cgaacgagg 
      ttgatgaaa tggaaaaat cgataacaa aaccctaac 
      gataaatat gtcaagttt caacaagat ataagtata 
      tatccataa tattataac a