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Gene information
General information
TC008603 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 15686643..15694826
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008603
    iBeetle screen iB_01369

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008603 RA
      MEPHDYIDK TVEILKNRF YFAVSKFSV YKRLKNSKN 
      YQFVCFDEE LRYRNFFHD FGPLNLACL YKYSCKITK 
      LLQNFRGRK KIVHYTSHD PNKKANAAF LVGCFAVVC 
      MKMEPFEVY KILLITEPY KQFVDALQR TSVHTIRLF 
      DCFSGVAKA LQYNLFDFT DFNVQEYDV CDQVEKGNM 
      NWIIPRKFL AFLGPTEPR VGVGHTPGF YLEYFLQHD 
      VKTVIRLND KLYDSSVFT RMGIEHHDL FFDDGSVPS 
      MDILLSFLR ITETAPAAI AVHCKAGLG RTGTLIGAY 
      LMKHYSMTA KEAVAWLRV CRPGSVTGA QQAYLEDNE 
      GWLWRAGSE YRIRHYGDE TRMPQHKYG VYSKQWPVD 
      RSRIINEAR RKLRWNDLN SHYRALRQL DEKQYLRHV 
      SKLLASVKG YEAEKLRDK VGKVLTGMP KY
    CDS sequence
    • >TC008603 RA
      atggaacca cacgactac atcgacaag actgttgaa 
      atcctcaag aaccgtttt tatttcgcc gtctcaaaa 
      ttctctgtt tacaagcgt ttgaaaaat tccaaaaac 
      tatcaattc gtgtgcttc gacgaagag ctcagatac 
      cggaacttt ttccacgat tttgggcca ctgaacctc 
      gcctgtctg tacaaatat tcatgcaaa attaccaag 
      ttgttgcag aatttcaga gggaggaag aaaattgtc 
      cattacacg agccatgat cccaataaa aaagccaac 
      gcggccttt cttgtcggc tgtttcgcc gtcgtttgc 
      atgaaaatg gaaccgttt gaagtgtac aaaattcta 
      ctcatcaca gaaccatac aaacaattt gttgacgcc 
      cttcagcgc acttcagtt catacaatt cgacttttt 
      gactgtttt tcaggagtt gcaaaagcg ttacaatac 
      aatttgttc gatttcacc gattttaat gttcaagaa 
      tacgatgtg tgtgatcaa gtggagaaa ggaaatatg 
      aactggatc atccctcgg aaatttctc gcttttttg 
      gggcccact gaaccaagg gtcggagtt ggccacact 
      cccgggttt tatttggag tatttcctt caacacgat 
      gtcaagact gtgattcgg ctcaatgac aaactttac 
      gactcatcg gtttttact cggatgggg atagagcat 
      cacgattta ttttttgac gacggttca gttccatca 
      atggacatt ttgctcagc tttttgcga attactgaa 
      accgctcca gcagcaatc gcagttcat tgcaaagct 
      gggcttggg agaaccggg actttgata ggggcttac 
      ctcatgaaa cattattca atgactgcg aaggaggca 
      gtggcttgg ttgagggtt tgtcggcct gggtctgtg 
      acgggggcc caacaggcc tacttggaa gacaacgag 
      ggctggctt tggagggca gggagtgaa tacaggatc 
      agacattat ggggatgag actagaatg cctcagcat 
      aaatacgga gtttacagc aagcaatgg cccgttgat 
      cgaagtcgg attataaat gaggctagg agaaaattg 
      aggtggaac gatcttaat tcccattat cgtgcttta 
      agacaactg gacgaaaaa cagtatttg agacatgta 
      tccaaatta ttggcttca gtaaagggc tacgaggcg 
      gagaagttg agagataag gttgggaaa gtccttaca 
      ggaatgccg aaatattaa
    mRNA sequence
    • >TC008603 RA
      gtgtgcttc tgtcttcag tcaaaaaat gcaaaattc 
      ataaaatga agcaaatca ctcatcaaa taatcggtt 
      tttctttgg cattactta taaattaca cgttacagt 
      ttctctttt tcgcggcat tgtgcagtg attcaaatt 
      attgctatt tatttcctt aaagaaaca aaatggaac 
      cacacgact acatcgaca agactgttg aaatcctca 
      agaaccgtt tttatttcg ccgtctcaa aattctctg 
      tttacaagc gtttgaaaa attccaaaa actatcaat 
      tcgtgtgct tcgacgaag agctcagat accggaact 
      ttttccacg attttgggc cactgaacc tcgcctgtc 
      tgtacaaat attcatgca aaattacca agttgttgc 
      agaatttca gagggagga agaaaattg tccattaca 
      cgagccatg atcccaata aaaaagcca acgcggcct 
      ttcttgtcg gctgtttcg ccgtcgttt gcatgaaaa 
      tggaaccgt ttgaagtgt acaaaattc tactcatca 
      cagaaccat acaaacaat ttgttgacg cccttcagc 
      gcacttcag ttcatacaa ttcgacttt ttgactgtt 
      tttcaggag ttgcaaaag cgttacaat acaatttgt 
      tcgatttca ccgatttta atgttcaag aatacgatg 
      tgtgtgatc aagtggaga aaggaaata tgaactgga 
      tcatccctc ggaaatttc tcgcttttt tggggccca 
      ctgaaccaa gggtcggag ttggccaca ctcccgggt 
      tttatttgg agtatttcc ttcaacacg atgtcaaga 
      ctgtgattc ggctcaatg acaaacttt acgactcat 
      cggttttta ctcggatgg ggatagagc atcacgatt 
      tattttttg acgacggtt cagttccat caatggaca 
      ttttgctca gctttttgc gaattactg aaaccgctc 
      cagcagcaa tcgcagttc attgcaaag ctgggcttg 
      ggagaaccg ggactttga taggggctt acctcatga 
      aacattatt caatgactg cgaaggagg cagtggctt 
      ggttgaggg tttgtcggc ctgggtctg tgacggggg 
      cccaacagg cctacttgg aagacaacg agggctggc 
      tttggaggg cagggagtg aatacagga tcagacatt 
      atggggatg agactagaa tgcctcagc ataaatacg 
      gagtttaca gcaagcaat ggcccgttg atcgaagtc 
      ggattataa atgaggcta ggagaaaat tgaggtgga 
      acgatctta attcccatt atcgtgctt taagacaac 
      tggacgaaa aacagtatt tgagacatg tatccaaat 
      tattggctt cagtaaagg gctacgagg cggagaagt 
      tgagagata aggttggga aagtcctta caggaatgc 
      cgaaatatt aattattgt aactatgac tttggaaat 
      aaatatttt tcggttttt gtataaata aatttcttt 
      atgactg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY