Status
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Gene information
General information
TC031216
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC004042,TC008595,TC008597,TC008596
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 16194159..16263160
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031216
    iBeetle screen iB_00657

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 3
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    iBeetle screen iB_01368

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Fertility
    egg production: number no/almost no eggs -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031216 RA
      MYKLLVLVG ALASAYGFL LSSKASNCT DNDFFCQNF 
      ECVPSKMQC DGNPDCSDG SDEHDCDMF HCASPDFFR 
      CKNSRCISS AFVCDLEND CDDFSDEEN CEEFKKKLE 
      KNSTCTRDQ WQCTDKLCI PLEWVCNGE PDCLDGSDE 
      ALGCSHTME CNDGFKCKN GHCIFKEWR CDGQDDCRD 
      NSDEEDCES HFELKECTL ENQRFLCSD TKTCIKLSE 
      VCDEVNHCP DKSDEGFSC KKTCENCSH NCVQLPTGP 
      KCLCPKGYR NIDEKHCQD INECEIYGI CDQKCKNTP 
      GSFECYCED NYNLQEDKK SCKAHFGEA LMFFSSKDQ 
      IRAYLLRSE LYFPVAKNL NQVVGVDFD GLYVYWTDI 
      LSEHESIVR SLKDGEQKE LLVTAGLGA PEDLHVDYI 
      TRNIYFTDA ERQHIGVCT NDGSYCTVL VTKDVQKPR 
      AIVLNSLEG EMYWTDWGH KPHIARALM DGTNGSSFI 
      SSDIHWPNG LTIDYPNSR LYWTDAKLM TLESIRLDG 
      TDRRIVLNE IVKHPYAIA VFENRLYWS DWSTHSIQS 
      CDKFTGKNH HTIIKEQKE YIYGITIYH PNNHKTPHL 
      NPCVHKPCS DICLLSGPQ GFTCACPQN KELSSNGFT 
      CRDLEKKQK LVLGAGNLL IEVEHQVLG RHEVNAMPI 
      TVKKMGALT YSSVENVIY VSDLELRKI ISVNLHTEM 
      AKPIDVGSL GHVTAMDYD YLGNNLYWC DSLRDTIEV 
      YNFNTNSRK ILLHDTNGE TPESIALVP EEGIMFVAF 
      RRFGQRDGH VDRFLMDGT SRTHPIENG LLGPISLYY 
      DSDIHRILI ADSGTRNIE STSVEGDDR HGFRSLQTN 
      PIGITSLKS EVFWVNRNS KELYWADKY NSGDDDYNK 
      KITLDLPDD IDRMYLVSV TPRKIGLSP CQRSNGGCS 
      HLCLQSHKT IVCACPVDM SLDSTNKTC IKRIDCTSH 
      EFFCSGSNS CIFKKFRCD GERNCPNGE DETDCKELN 
      HCPWNNFQC HDGECIDER FKCDYKFDC RDKSDERNC 
      SIDAKKCPP GHFMCKSGQ CINERLVCD GVKDCLEEE 
      DEANCVSTV CKDYEFRCQ SGACIPKNW ECDHDYDCP 
      DFSDEHSGC ASCDASTFT CNNGKCIDK SFVCDKEND 
      CSDNSDELS CVMENSCDL SEFSCSLHT HICLPDSAR 
      CNGTSECPH HEDEQNCSN CQVDEFSCN NTKCIPREW 
      ICDHSDDCG DGSDEVPSL CNHTIPEHA TNFSCIDRF 
      RCRNGNCID FSLVCNKEP NCYDGSDEE GLCNTSCSA 
      LNNPCSQIC VKTPTGPTC KCQGGYQLR GDGHTCEDI 
      NECAEHPPV CSQICHNSE GSYSCDCYE GFALRTDRT 
      SCKAKGTAM SLIFSSNNQ IREISQLEN SLKMLYSEE 
      TPRITSLDV GLTSGAIYF TVENSNAIL KINKGDTKR 
      EYLENVGQP CKITLDWRT NNIYYFNNA PTTKSIGLC 
      NFNDKTCAN LIPVDTHRQ VSALVVDPI NNVLFYSLV 
      SWWIFNSPS YIVYKTNLD GTGGQELIK TSPGYITGL 
      SYDLYKKEL YFISQHQLS KIKYDGSDR TVLLSNLTQ 
      SVGLNFFEN HLYFKSGNF MQKCRLYSP ITCETFGLK 
      GDVFAIAQE SRQPEFGNN PCQNHTCNQ LCIPAKPQY 
      RCLCENGKF VEPEEDCDT LMREANDDG RRVKALHYV 
      NNYASSNLG PVIAGVMIS ICLVTFGAV LYYLIKRRN 
      SGTFNVISM KFHNPGYGL HTDEFDRSI LKPGQHEYE 
      NPVEFYGSE NDNHTNIQV NV
    CDS sequence
    • >TC031216 RA
      atgtacaag ctcctggtt ctcgtagga gctctagct 
      tctgcctac ggttttctc ctctcttct aaagcctca 
      aattgcacc gataacgat tttttctgc caaaatttc 
      gaatgcgtc ccttcgaaa atgcaatgt gacggtaat 
      cccgattgc agcgatggt tccgatgag catgattgc 
      gatatgttt cactgtgct tcgccggat ttttttcgc 
      tgcaaaaac tcgcgatgt atcagttct gcgttcgtg 
      tgcgacttg gaaaacgac tgcgatgac ttttccgat 
      gaggaaaat tgtgaagag tttaaaaaa aagcttgag 
      aagaactcc acgtgtaca agggaccaa tggcaatgt 
      actgataag ttgtgtatt ccactggaa tgggtgtgc 
      aatggggaa cctgattgc ttggatggg tcagatgaa 
      gcgctgggt tgtagccat accatggaa tgcaatgat 
      ggtttcaag tgcaagaac ggacattgc atttttaag 
      gaatggagg tgtgatgga caagatgat tgcagagat 
      aacagcgat gaagaagat tgcgaaagt cattttgaa 
      cttaaagaa tgtactttg gaaaaccag agatttctc 
      tgttctgat actaaaact tgtatcaaa ttgagtgaa 
      gtgtgcgac gaggtgaac cactgtcct gataaatcc 
      gatgaaggg ttttcctgc aagaaaacc tgcgaaaat 
      tgttcgcac aactgcgtc cagctacca acagggcct 
      aaatgcttg tgtcctaaa gggtaccgc aacattgat 
      gagaaacac tgtcaagac atcaacgag tgcgaaata 
      tacggaatt tgtgaccag aaatgtaaa aacacacca 
      ggctcgttt gagtgttat tgtgaggac aattacaac 
      ctccaggag gataaaaaa agttgcaag gctcatttt 
      ggcgaagct ttgatgttt ttcagttca aaagaccaa 
      atccgggct tatttgttg cgttccgag ttgtatttt 
      ccagtagct aaaaatctg aatcaagtg gttggagtt 
      gattttgat ggattgtat gtctactgg actgatatt 
      ttatcggaa catgagagt atagtcagg tcgttgaaa 
      gacggggaa cagaaagag ttgttggta actgccggt 
      ttgggtgcc cctgaagac ctccacgta gactacatt 
      accagaaat atctacttc actgatgcg gagcgtcaa 
      catatcgga gtttgtacc aacgatggg tcgtactgt 
      accgttcta gtgaccaaa gatgtgcaa aaacctaga 
      gctattgtc ctcaattca ttagaaggg gaaatgtac 
      tggacggat tggggtcac aagccccat atcgcccgc 
      gccctcatg gatggtacc aatggtagt tcgttcatc 
      tcaagtgat atccattgg cccaatggt ctaaccatc 
      gattatcca aactctcgt ctctactgg actgatgcc 
      aaactcatg acccttgaa agcatacga cttgatggt 
      actgaccgc aggattgtc ctcaatgaa atcgttaaa 
      cacccatac gccatcgct gttttcgaa aaccgtctg 
      tattggtca gattggtcc actcactcc atccaatcg 
      tgcgataaa ttcaccgga aagaaccac cataccata 
      atcaaggaa cagaaggag tatatttat ggcatcacc 
      atctatcat cctaacaac cataagaca ccacatttg 
      aacccatgt gtacacaaa ccttgcagc gatatttgt 
      ctactgagt ggcccccag ggtttcact tgtgcttgt 
      ccccaaaac aaggaacta agctcaaat ggattcacg 
      tgtagagat ttggaaaag aaacaaaaa ttagtactt 
      ggagccgga aaccttcta atagaagtt gaacatcag 
      gttttgggt cgacatgaa gtcaatgct atgccgata 
      acggtcaaa aaaatggga gcattgact tacagcagt 
      gtggaaaat gtcatctac gttagcgat ttggaatta 
      agaaaaatt ataagtgtt aatctgcac acagaaatg 
      gccaaacca atcgatgtt ggaagtctg ggtcatgtt 
      acagctatg gattacgat tatctgggg aataacctg 
      tactggtgc gactctcta cgtgatacc atcgaagtc 
      tacaatttt aacaccaac tcccggaaa attttgcta 
      cacgatacg aatggcgaa actcccgaa tctattgct 
      ctagtgcct gaagaaggg atcatgttt gtagctttt 
      cgaagattt gggcaacga gatggtcac gtggatagg 
      tttcttatg gatggtacg agcagaacc catcccatt 
      gaaaatgga cttcttggc cccatatct ctttattac 
      gatagtgac attcatcgt atcctcatt gccgattct 
      ggtacccga aatattgaa agtacgagt gttgaaggt 
      gatgacaga catggattc cgatcactt caaaccaac 
      ccaattggg ataacttca cttaaatca gaggttttc 
      tgggttaac cgcaattcg aaggagttg tattgggcc 
      gataaatac aacagtggg gatgatgat tacaacaaa 
      aaaatcact ttagatctc cccgatgat attgatcga 
      atgtattta gtatcagtc actcctcgt aaaattggg 
      ttaagtcca tgtcagcga agcaacgga ggttgttca 
      cacttatgt ctccagtcg cacaagaca atagtttgt 
      gcttgccca gtcgatatg agtcttgat tccaccaac 
      aaaacttgc atcaaacga attgattgt acttcgcat 
      gagtttttc tgtagtggt tccaacagt tgcatcttc 
      aagaaattc agatgcgat ggtgaaagg aattgtcca 
      aatggagaa gacgaaacc gattgcaag gaattaaat 
      cattgtcct tggaacaac ttccagtgc catgacggg 
      gagtgtatt gatgagaga ttcaaatgc gattacaaa 
      tttgactgc agggataaa tcagatgaa cgcaactgt 
      agcattgat gcgaaaaaa tgccctcct ggacatttc 
      atgtgcaaa agtgggcaa tgtatcaat gaacgcctc 
      gtctgcgat ggtgttaaa gactgtctc gaggaggaa 
      gacgaagct aattgtgtc tcaacagtg tgcaaagac 
      tacgaattc agatgtcaa tcaggtgct tgcatcccg 
      aaaaactgg gaatgcgac catgactac gactgtcca 
      gacttttcc gatgaacat tccggttgt gcttcttgt 
      gacgcctct actttcact tgcaacaat ggcaaatgt 
      atcgacaaa agcttcgtc tgcgacaag gaaaatgat 
      tgtagtgac aactcggat gaactttca tgcgtcatg 
      gaaaattcc tgcgatttg agcgaattt tcctgctct 
      ttgcacacc cacatttgt cttccggac tcagcgcgt 
      tgtaatgga acgtccgaa tgcccacac catgaagac 
      gaacaaaac tgttccaat tgtcaagtc gatgaattt 
      tcctgcaat aacacaaag tgtatcccg agggaatgg 
      atttgtgac cattcggat gattgtggc gacggttca 
      gatgaagtc ccttcgttg tgtaaccac acaattccg 
      gaacatgcc acgaatttt tcttgtatt gatcgcttc 
      cggtgcagg aatgggaat tgtatcgat ttctccttg 
      gtgtgtaat aaggaacct aattgttac gacggatcg 
      gatgaagaa gggctttgt aacacatct tgtagcgct 
      ctgaataac ccttgctca caaatttgt gtcaaaaca 
      ccaacagga cccacctgt aaatgtcaa ggaggatac 
      cagctccga ggagatggt catacctgt gaagatatc 
      aatgagtgt gcggagcat cccccggtt tgcagccaa 
      atttgccac aactcggaa ggatcatac tcgtgtgat 
      tgttacgaa ggatttgct ctaagaaca gataggaca 
      agctgtaag gccaaaggc acggcaatg tcactcatt 
      ttttcgtcg aataatcaa attcgagaa atatcacaa 
      ctagaaaat tccctcaag atgctttac tcggaggaa 
      actcccaga attacaagt ctagatgta gggctcacg 
      tctggagcc atttatttc acagttgag aattcaaat 
      gctatactt aaaataaac aaaggtgat acgaaacga 
      gagtacttg gaaaatgtg gggcaacca tgcaaaatc 
      actcttgat tggaggaca aacaacata tactacttt 
      aataacgcc ccgacgacc aaatcaatc ggactttgc 
      aattttaat gataaaacg tgtgcaaat ctcatccca 
      gttgacact cacagacag gtctcagcc ttagttgtg 
      gaccctatc aacaacgtc ctcttctat tctcttgtc 
      agttggtgg attttcaat tccccaagt tacatcgtc 
      tacaagaca aaccttgac ggaacagga ggccaagaa 
      ctgatcaaa acctctccc ggttacatc acaggactt 
      tcctacgat ttatacaag aaagaactc tatttcatc 
      agccaacat caactgagc aaaatcaaa tacgacggt 
      agtgataga accgttctg ttgtcaaac ttgacccaa 
      tcagttggt ttaaatttc ttcgagaat catttgtac 
      ttcaaaagt ggcaatttc atgcaaaag tgtcgtttg 
      tactcgccg attacttgt gaaacattc ggtctgaaa 
      ggtgacgta tttgctatc gcccaagag tcacgacag 
      ccggagttt gggaataat ccatgtcaa aatcatact 
      tgtaaccag ctttgtatc cctgcaaag ccacagtat 
      cggtgtttg tgcgaaaat ggcaaattt gtagaacca 
      gaggaggat tgcgacact ttgatgagg gaagcaaat 
      gatgatgga agaagagta aaagctctc cattacgta 
      aataattac gcaagttca aatttgggg cctgtgatt 
      gccggtgtg atgatttcc atctgtttg gtcactttt 
      ggtgctgtt ctttactat ttgataaaa agacgcaac 
      agtggaacc tttaacgtg attagcatg aagtttcat 
      aatccgggc tatggtttg cacacagat gaattcgat 
      aggtcgatt cttaaaccc ggacaacat gaatatgaa 
      aatccggtt gaattttac ggttctgaa aatgataac 
      cacaccaat atccaagtt aacgtttaa
    mRNA sequence
    • >TC031216 RA
      catctcaac aactacaaa gcacacatc gatcacaca 
      ctttagcat gtacaagct cctggttct cgtaggagc 
      tctagcttc tgcctacgg ttttctcct ctcttctaa 
      agcctcaaa ttgcaccga taacgattt tttctgcca 
      aaatttcga atgcgtccc ttcgaaaat gcaatgtga 
      cggtaatcc cgattgcag cgatggttc cgatgagca 
      tgattgcga tatgtttca ctgtgcttc gccggattt 
      ttttcgctg caaaaactc gcgatgtat cagttctgc 
      gttcgtgtg cgacttgga aaacgactg cgatgactt 
      ttccgatga ggaaaattg tgaagagtt taaaaaaaa 
      gcttgagaa gaactccac gtgtacaag ggaccaatg 
      gcaatgtac tgataagtt gtgtattcc actggaatg 
      ggtgtgcaa tggggaacc tgattgctt ggatgggtc 
      agatgaagc gctgggttg tagccatac catggaatg 
      caatgatgg tttcaagtg caagaacgg acattgcat 
      ttttaagga atggaggtg tgatggaca agatgattg 
      cagagataa cagcgatga agaagattg cgaaagtca 
      ttttgaact taaagaatg tactttgga aaaccagag 
      atttctctg ttctgatac taaaacttg tatcaaatt 
      gagtgaagt gtgcgacga ggtgaacca ctgtcctga 
      taaatccga tgaagggtt ttcctgcaa gaaaacctg 
      cgaaaattg ttcgcacaa ctgcgtcca gctaccaac 
      agggcctaa atgcttgtg tcctaaagg gtaccgcaa 
      cattgatga gaaacactg tcaagacat caacgagtg 
      cgaaatata cggaatttg tgaccagaa atgtaaaaa 
      cacaccagg ctcgtttga gtgttattg tgaggacaa 
      ttacaacct ccaggagga taaaaaaag ttgcaaggc 
      tcattttgg cgaagcttt gatgttttt cagttcaaa 
      agaccaaat ccgggctta tttgttgcg ttccgagtt 
      gtattttcc agtagctaa aaatctgaa tcaagtggt 
      tggagttga ttttgatgg attgtatgt ctactggac 
      tgatatttt atcggaaca tgagagtat agtcaggtc 
      gttgaaaga cggggaaca gaaagagtt gttggtaac 
      tgccggttt gggtgcccc tgaagacct ccacgtaga 
      ctacattac cagaaatat ctacttcac tgatgcgga 
      gcgtcaaca tatcggagt ttgtaccaa cgatgggtc 
      gtactgtac cgttctagt gaccaaaga tgtgcaaaa 
      acctagagc tattgtcct caattcatt agaagggga 
      aatgtactg gacggattg gggtcacaa gccccatat 
      cgcccgcgc cctcatgga tggtaccaa tggtagttc 
      gttcatctc aagtgatat ccattggcc caatggtct 
      aaccatcga ttatccaaa ctctcgtct ctactggac 
      tgatgccaa actcatgac ccttgaaag catacgact 
      tgatggtac tgaccgcag gattgtcct caatgaaat 
      cgttaaaca cccatacgc catcgctgt tttcgaaaa 
      ccgtctgta ttggtcaga ttggtccac tcactccat 
      ccaatcgtg cgataaatt caccggaaa gaaccacca 
      taccataat caaggaaca gaaggagta tatttatgg 
      catcaccat ctatcatcc taacaacca taagacacc 
      acatttgaa cccatgtgt acacaaacc ttgcagcga 
      tatttgtct actgagtgg cccccaggg tttcacttg 
      tgcttgtcc ccaaaacaa ggaactaag ctcaaatgg 
      attcacgtg tagagattt ggaaaagaa acaaaaatt 
      agtacttgg agccggaaa ccttctaat agaagttga 
      acatcaggt tttgggtcg acatgaagt caatgctat 
      gccgataac ggtcaaaaa aatgggagc attgactta 
      cagcagtgt ggaaaatgt catctacgt tagcgattt 
      ggaattaag aaaaattat aagtgttaa tctgcacac 
      agaaatggc caaaccaat cgatgttgg aagtctggg 
      tcatgttac agctatgga ttacgatta tctggggaa 
      taacctgta ctggtgcga ctctctacg tgataccat 
      cgaagtcta caattttaa caccaactc ccggaaaat 
      tttgctaca cgatacgaa tggcgaaac tcccgaatc 
      tattgctct agtgcctga agaagggat catgtttgt 
      agcttttcg aagatttgg gcaacgaga tggtcacgt 
      ggataggtt tcttatgga tggtacgag cagaaccca 
      tcccattga aaatggact tcttggccc catatctct 
      ttattacga tagtgacat tcatcgtat cctcattgc 
      cgattctgg tacccgaaa tattgaaag tacgagtgt 
      tgaaggtga tgacagaca tggattccg atcacttca 
      aaccaaccc aattgggat aacttcact taaatcaga 
      ggttttctg ggttaaccg caattcgaa ggagttgta 
      ttgggccga taaatacaa cagtgggga tgatgatta 
      caacaaaaa aatcacttt agatctccc cgatgatat 
      tgatcgaat gtatttagt atcagtcac tcctcgtaa 
      aattgggtt aagtccatg tcagcgaag caacggagg 
      ttgttcaca cttatgtct ccagtcgca caagacaat 
      agtttgtgc ttgcccagt cgatatgag tcttgattc 
      caccaacaa aacttgcat caaacgaat tgattgtac 
      ttcgcatga gtttttctg tagtggttc caacagttg 
      catcttcaa gaaattcag atgcgatgg tgaaaggaa 
      ttgtccaaa tggagaaga cgaaaccga ttgcaagga 
      attaaatca ttgtccttg gaacaactt ccagtgcca 
      tgacgggga gtgtattga tgagagatt caaatgcga 
      ttacaaatt tgactgcag ggataaatc agatgaacg 
      caactgtag cattgatgc gaaaaaatg ccctcctgg 
      acatttcat gtgcaaaag tgggcaatg tatcaatga 
      acgcctcgt ctgcgatgg tgttaaaga ctgtctcga 
      ggaggaaga cgaagctaa ttgtgtctc aacagtgtg 
      caaagacta cgaattcag atgtcaatc aggtgcttg 
      catcccgaa aaactggga atgcgacca tgactacga 
      ctgtccaga cttttccga tgaacattc cggttgtgc 
      ttcttgtga cgcctctac tttcacttg caacaatgg 
      caaatgtat cgacaaaag cttcgtctg cgacaagga 
      aaatgattg tagtgacaa ctcggatga actttcatg 
      cgtcatgga aaattcctg cgatttgag cgaattttc 
      ctgctcttt gcacaccca catttgtct tccggactc 
      agcgcgttg taatggaac gtccgaatg cccacacca 
      tgaagacga acaaaactg ttccaattg tcaagtcga 
      tgaattttc ctgcaataa cacaaagtg tatcccgag 
      ggaatggat ttgtgacca ttcggatga ttgtggcga 
      cggttcaga tgaagtccc ttcgttgtg taaccacac 
      aattccgga acatgccac gaatttttc ttgtattga 
      tcgcttccg gtgcaggaa tgggaattg tatcgattt 
      ctccttggt gtgtaataa ggaacctaa ttgttacga 
      cggatcgga tgaagaagg gctttgtaa cacatcttg 
      tagcgctct gaataaccc ttgctcaca aatttgtgt 
      caaaacacc aacaggacc cacctgtaa atgtcaagg 
      aggatacca gctccgagg agatggtca tacctgtga 
      agatatcaa tgagtgtgc ggagcatcc cccggtttg 
      cagccaaat ttgccacaa ctcggaagg atcatactc 
      gtgtgattg ttacgaagg atttgctct aagaacaga 
      taggacaag ctgtaaggc caaaggcac ggcaatgtc 
      actcatttt ttcgtcgaa taatcaaat tcgagaaat 
      atcacaact agaaaattc cctcaagat gctttactc 
      ggaggaaac tcccagaat tacaagtct agatgtagg 
      gctcacgtc tggagccat ttatttcac agttgagaa 
      ttcaaatgc tatacttaa aataaacaa aggtgatac 
      gaaacgaga gtacttgga aaatgtggg gcaaccatg 
      caaaatcac tcttgattg gaggacaaa caacatata 
      ctactttaa taacgcccc gacgaccaa atcaatcgg 
      actttgcaa ttttaatga taaaacgtg tgcaaatct 
      catcccagt tgacactca cagacaggt ctcagcctt 
      agttgtgga ccctatcaa caacgtcct cttctattc 
      tcttgtcag ttggtggat tttcaattc cccaagtta 
      catcgtcta caagacaaa ccttgacgg aacaggagg 
      ccaagaact gatcaaaac ctctcccgg ttacatcac 
      aggactttc ctacgattt atacaagaa agaactcta 
      tttcatcag ccaacatca actgagcaa aatcaaata 
      cgacggtag tgatagaac cgttctgtt gtcaaactt 
      gacccaatc agttggttt aaatttctt cgagaatca 
      tttgtactt caaaagtgg caatttcat gcaaaagtg 
      tcgtttgta ctcgccgat tacttgtga aacattcgg 
      tctgaaagg tgacgtatt tgctatcgc ccaagagtc 
      acgacagcc ggagtttgg gaataatcc atgtcaaaa 
      tcatacttg taaccagct ttgtatccc tgcaaagcc 
      acagtatcg gtgtttgtg cgaaaatgg caaatttgt 
      agaaccaga ggaggattg cgacacttt gatgaggga 
      agcaaatga tgatggaag aagagtaaa agctctcca 
      ttacgtaaa taattacgc aagttcaaa tttggggcc 
      tgtgattgc cggtgtgat gatttccat ctgtttggt 
      cacttttgg tgctgttct ttactattt gataaaaag 
      acgcaacag tggaacctt taacgtgat tagcatgaa 
      gtttcataa tccgggcta tggtttgca cacagatga 
      attcgatag gtcgattct taaacccgg acaacatga 
      atatgaaaa tccggttga attttacgg ttctgaaaa 
      tgataacca caccaatat ccaagttaa cgtttaatg 
      tgttagtga ttattttaa tgatgtttt atactaaat 
      tgttgtatc gttgttagt cgcagtcgt tttatttat 
      cttattaat ttatattta tagtaaaat attaaattt 
      tatgaatgg tt
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