Status
Please wait ...
Gene information
General information
TC008569 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 1481056..1490141
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008569
    iBeetle screen iB_01366

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008569 RA
      MVVTSIARR LLFFIFYNA VLATAFHWD YKHEDEWPD 
      QCQKGKQQS PINLSRQMA TETLFPSFI FEYYDEIYR 
      AEIKNSGHT AQIQLKVAV KPKVSGGGL LSTYVLDNM 
      HFHWNSEHT INEERFPLE LHLVHYDST KKSLAEALK 
      VKGGVAVLA VFFYLSPDS DSEFDSLFD TLDQLNKVK 
      LNEVKPIER LDLGDFLPR DRAGFYRYE GSLTTPNCT 
      EGVIWTVFT NGLPISSKQ LKIFSNLKT ESGGNIIKN 
      YRTLQELND RKVQIKISP IESSTASHV AIPSVLLLY 
      VGFFIVGDK L
    CDS sequence
    • >TC008569 RA
      atggttgtg acttcgata gcgcgtcgg cttttattt 
      ttcatcttt tataatgca gttttggcg actgctttc 
      cattgggat tacaaacat gaagacgaa tggcctgat 
      caatgtcaa aagggaaaa cagcagtct cccattaat 
      ttgtcacgt caaatggcc accgaaact ctctttcca 
      tcatttatt tttgaatac tacgatgaa atatacaga 
      gcggaaatc aagaacagt ggacataca gctcaaatt 
      cagcttaaa gtagcagta aagccgaag gtgagcggg 
      ggaggatta ctgtctact tatgtatta gacaacatg 
      cattttcac tggaactca gagcacacc ataaatgaa 
      gaaagattt cctctggaa ctacattta gtccattat 
      gattctact aagaaaagt ttagctgaa gccttaaaa 
      gtaaaggga ggagttgct gttttggct gtatttttc 
      tatctttcc cctgatagt gacagcgaa ttcgactct 
      ttatttgat accttggat cagctaaat aaagtcaag 
      ctaaacgaa gttaaacct attgaaaga ttagattta 
      ggggacttt cttccaaga gatagagct gggttttat 
      cgatacgaa ggatcctta accacaccg aattgtaca 
      gaaggagta atttggacc gtttttaca aatggtttg 
      cctatttcc agtaaacag ctgaaaatc ttctccaat 
      ttgaaaacg gaatcaggc ggcaacatt attaaaaat 
      tatagaaca ttacaagag ctcaatgac agaaaagtt 
      caaattaaa ataagtcct attgaatcc tcaactgca 
      tctcatgtt gctattcct agtgtttta ttattatat 
      gttggattt tttattgta ggtgataag ctctga
    mRNA sequence
    • >TC008569 RA
      gagacctct gtatttcgt aaacgaaga acaaaagat 
      ctacttctt tttgcattt ttgccatct ccattgctg 
      ttaccacaa gcacctttc ccgtggaaa ggattaggt 
      tgtgactta aattatcac gaataaaca acacagagc 
      catggcgtt atcaagaaa attaatttt gaaaatgtc 
      tgaacaaat aatttgagg tcacagaat agccatagg 
      tcacataag taatattta atatcgatg ctttgttta 
      tataaatta tgttaattt ctccgtatt gaagtagag 
      ataatttta ctatattta cattttgca agagatagt 
      ttgaagcag cacttacgt gaattttgt ttaacgttt 
      ttggtcaaa ataagttcc attttattt cagttctac 
      gtgacagtg attttacat gccacgagg ccaaatatc 
      attaacttt aataggtac gattgtaga atatgaagc 
      aggcactca aaacgttat aacattgaa gccacaata 
      cagtgacaa aattttaat gtgtaaaat ttttcgttt 
      tgatattag tttgttgaa aatataatt gaagacgat 
      aataataat taatgatta gaatgttga catgtaata 
      aataattat tgcgaccta tttttcatg tttattagc 
      aaaaattta cattttttg cgttatcta gtaaaacca 
      aaagatatc gcatgtatg tgttcatta gttattgtt 
      attttccat aataactag ccttgaaat tacattaat 
      atgatacat tttcaaaaa atgtcaaca attggaaac 
      ttccctttg atgggtact aatataatg ctaggtttc 
      aaaagcaaa gacggttta agcgctttg ataatacag 
      agattatat gcaataaag actcaagac attatgtaa 
      ataaattaa taaggaaga caatttatt aggagagtt 
      agtcttagt tatgtcgtt acaacaacg acaataatt 
      tattcattg tcaaattta caaatgtat agttttcgg 
      ttttgtgaa ttaatattt ttaaaattt attgcactt 
      ggtgaccat tggctgcag ttaggcatt gaaatgtaa 
      gctaaattt atcttgacg aatttattg ccgcatcgg 
      ataagtgag ttaaattta aattgggga gaccaaatg 
      gtagtactc ttcttaaag atttctttg ttagtaaca 
      aactaaatt aagctaaaa ttaaggtat cattgttgt 
      ctccaaaaa aaaacgtca aaatgacat taaaaattc 
      tagtttact ttgtactgt aacttatat taataaatt 
      gttatcttt attgctatt catttaaat aacaagtgt 
      acaaaaagg atcaccgct agttcagtt tatttttca 
      attgagaca ttggggggc agtttgcca acacagtga 
      gatttcttt ggaggtagc aacgactgt gaaatttag 
      ttttattag ttgaaacat ggttgtgac ttcgatagc 
      gcgtcggct tttattttt catctttta taatgcagt 
      tttggcgac tgctttcca ttgggatta caaacatga 
      agacgaatg gcctgatca atgtcaaaa gggaaaaca 
      gcagtctcc cattaattt gtcacgtca aatggccac 
      cgaaactct ctttccatc atttatttt tgaatacta 
      cgatgaaat atacagagc ggaaatcaa gaacagtgg 
      acatacagc tcaaattca gcttaaagt agcagtaaa 
      gccgaaggt gagcggggg aggattact gtctactta 
      tgtattaga caacatgca ttttcactg gaactcaga 
      gcacaccat aaatgaaga aagatttcc tctggaact 
      acatttagt ccattatga ttctactaa gaaaagttt 
      agctgaagc cttaaaagt aaagggagg agttgctgt 
      tttggctgt atttttcta tctttcccc tgatagtga 
      cagcgaatt cgactcttt atttgatac cttggatca 
      gctaaataa agtcaagct aaacgaagt taaacctat 
      tgaaagatt agatttagg ggactttct tccaagaga 
      tagagctgg gttttatcg atacgaagg atccttaac 
      cacaccgaa ttgtacaga aggagtaat ttggaccgt 
      ttttacaaa tggtttgcc tatttccag taaacagct 
      gaaaatctt ctccaattt gaaaacgga atcaggcgg 
      caacattat taaaaatta tagaacatt acaagagct 
      caatgacag aaaagttca aattaaaat aagtcctat 
      tgaatcctc aactgcatc tcatgttgc tattcctag 
      tgttttatt attatatgt tggattttt tattgtagg 
      tgataagct ctgacaaga ttttttact aaccataag 
      ctgaatatt atttattat tacaagtgt acctgtttg 
      aatctaaat acattggtg tctaatgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY